RESUMEN
OBJECTIVE: In order to evaluate the potential zoonotic transmission of Giardia duodenalis, isolates from humans and dogs in the Northwestern region of the São Paulo State, Brazil were characterized based on the ß-giardin gene. METHODS: The samples were analyzed by sequencing of the Nested-PCR products. RESULTS: The A1 and A2 subgenotypes were detected in human and dogs. Cysts of assemblage B, C and D have not been found in any isolates studied. CONCLUSIONS: These results are consistent with the view that giardiasis in the largest endemic region of the Brazil should not be seen as a single entity.
Asunto(s)
Heces/parasitología , Giardia/genética , Giardiasis/transmisión , Proteínas Protozoarias/genética , Zoonosis/parasitología , Animales , Brasil , Enfermedades de los Perros/parasitología , Enfermedades de los Perros/transmisión , Perros , Genotipo , Giardia/aislamiento & purificación , Giardiasis/diagnóstico , Giardiasis/veterinaria , Humanos , Reacción en Cadena de la PolimerasaRESUMEN
OBJECTIVE: In order to evaluate the potential zoonotic transmission of Giardia duodenalis, isolates from humans and dogs in the Northwestern region of the São Paulo State, Brazil were characterized based on the β-giardin gene. METHODS: The samples were analyzed by sequencing of the Nested-PCR products. RESULTS: The A1 and A2 subgenotypes were detected in human and dogs. Cysts of assemblage B, C and D have not been found in any isolates studied. CONCLUSIONS: These results are consistent with the view that giardiasis in the largest endemic region of the Brazil should not be seen as a single entity.