RESUMEN
PURPOSE: To provide a simple alternative acute ocular toxoplasmosis model with great reproducibility for experimental tests that demand monitoring of the ocular lesion. METHODS: ME49-wt and ME49-GFP tachyzoites from cell culture were used to infect male C57BL6 mice by intraperitoneal injection. B1 expression by real-time polymerase chain reaction (qPCR) assay was used to detect the presence of T. gondii in ocular tissue at the beginning of the infection. Fluorescence microscopy and histopathology analysis were carried out to assess the evolution of the acute infection up to 20 days in both eyes of infected mice. RESULTS: All mice infected with the 104 tachyzoites showed B1 expression in the retina of both eyes, in the RPE (retinal pigment epithelium), and choroid structures, after 5 days of infection. Tachyzoites of the ME49-GFP strain were easily detected by fluorescence microscopy in the retina tissue of mice after 5 days post-infection. After 20 days, mice inflammatory cell infiltrates and a disorganized morphology of the retinal laminar architecture were observed. CONCLUSION: Infection of C57BL6 mice via intraperitoneal with 104 tachyzoites of the ME49-GFP strain from cell culture is a suitable model for acute ocular toxoplasmosis. This model has great reproducibility in establishing the ocular lesion since day 5 post-infection. This model can be suitable for experimental tests of chemotherapy and the investigation of the role of the immune response on the development of uveitis.
Asunto(s)
Toxoplasmosis Ocular , Masculino , Animales , Ratones , Toxoplasmosis Ocular/diagnóstico , Reproducibilidad de los Resultados , Ratones Endogámicos C57BL , Retina , Epitelio Pigmentado de la RetinaRESUMEN
In mice, oral Toxoplasma gondii infection induces severe ileitis. The aim of the present study was to investigate the impact of the P2X7 receptor (P2X7) on the inflammatory response to T. gondii-induced ileitis. Cysts of the ME49 strain of T. gondii were used to induce ileitis. The infected mice were euthanized on day 8 and ileal tissue and peripheral blood were collected for histopathological and immunohistochemical analyses. Ileal contractility, inflammatory mediators, inflammasome activation, quantitative PCR analysis of gene expression, and fecal microbiota were assessed using appropriate techniques, respectively. The infected P2X7-/- mice had greater disease severity, parasitic burden, liver damage, and intestinal contractility than the infected wild-type (WT) mice. Infection increased serum IL-6 and IFN-γ and tissue caspase-1 but not NLRP3 in P2X7-/- mice compared to WT mice. Bacteroidaceae, Rikenellaceae, and Rhodospirillales increased while Muribaculaceae and Lactobacillaceae decreased in the infected WT and P2X7-/- mice. Bacteroidia and Tannerellaceae increased in the P2X7-/- mice with ileitis. By contrast, Clostridiales and Mollicutes were absent in the P2X7-/- mice but increased in the WT mice. P2X7 protects mice against T. gondii infection by activating the inflammasome and regulating the local and systemic immune responses. Specific gut bacterial populations modulated by P2X7 determine disease severity.
RESUMEN
BACKGROUND: Clostridioides difficile is the most common cause of nosocomial diarrhea associated with antibiotic use. The disease's symptoms are caused by enterotoxins, but other surface adhesion factors also play a role in the pathogenesis. These adhesins will bind to components of extracellular matrix. OBJECTIVE: There is a lack of knowledge on MSCRAMM, this work set-out to determine the adhesive properties of several C. difficile ribotypes (027, 133, 135, 014, 012) towards laminin-1 (LMN-1). METHODS: A binding experiment revealed that different ribotypes have distinct adhesion capabilities. To identify this adhesin, an affinity chromatography column containing LMN-1 was prepared and total protein extracts were analysed using mass spectrometry. FINDINGS: Strains from ribotypes 012 and 027 had the best adhesion when incubated with glucose supplementations (0.2%, 0.5%, and 1%), while RT135 had a poor adherence. The criteria were not met by RT014 and RT133. In the absence of glucose, there was no adhesion for any ribotype, implying that glucose is required and plays a significant role in adhesion. MAIN CONCLUSIONS: These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein.
Asunto(s)
Clostridioides difficile , Clostridioides , Matriz Extracelular , Glucosa , Laminina , RibotipificaciónRESUMEN
BACKGROUND Clostridioides difficile is the most common cause of nosocomial diarrhea associated with antibiotic use. The disease's symptoms are caused by enterotoxins, but other surface adhesion factors also play a role in the pathogenesis. These adhesins will bind to components of extracellular matrix. OBJECTIVE There is a lack of knowledge on MSCRAMM, this work set-out to determine the adhesive properties of several C. difficile ribotypes (027, 133, 135, 014, 012) towards laminin-1 (LMN-1). METHODS A binding experiment revealed that different ribotypes have distinct adhesion capabilities. To identify this adhesin, an affinity chromatography column containing LMN-1 was prepared and total protein extracts were analysed using mass spectrometry. FINDINGS Strains from ribotypes 012 and 027 had the best adhesion when incubated with glucose supplementations (0.2%, 0.5%, and 1%), while RT135 had a poor adherence. The criteria were not met by RT014 and RT133. In the absence of glucose, there was no adhesion for any ribotype, implying that glucose is required and plays a significant role in adhesion. MAIN CONCLUSIONS These findings show that in the presence of glucose, each C. difficile ribotype interacts differently with LMN-1, and the adhesin responsible for recognition could be SlpA protein.
RESUMEN
Surgical site infections in instrumented posterior lumbar interbody fusion surgery are normally due to gram-positive bacteria, but gram-negative bacteria can cause infections in cases involving lower lumbar interventions as its closer to the perianal area. Here we report an uncommon fatal wound infection caused by a multidrug-resistant Klebsiella pneumoniae after an elective spine surgery. In silico analysis revealed that LWI_ST16 belonged to ST16, an emergent international clone notable for its increased virulence potential. We also observed that this strain carried a conjugative IncF plasmid encoding resistance genes to beta-lactams (blaKPC-2 and blaOXA-1), tetracycline (tetA), aminoglycosides and fluoroquinolones (aac(6')-Ib-cr). The carbapenemase encoding gene blaKPC-2 was located on a Tn4401e transposon previously characterized to increase blaKPC expression. LWI_ST16 is a strong biofilm producer on polystyrene and capable of forming tower-like structures on a titanium device like the one inserted in the patient's spine. Our findings strengthen the valuable contribution of continuous surveillance of multidrug-resistant and high-risk K. pneumoniae clones to avoid unfavourable clinical outcomes.
Asunto(s)
Farmacorresistencia Bacteriana Múltiple , Infecciones por Klebsiella/microbiología , Klebsiella pneumoniae/efectos de los fármacos , Columna Vertebral/cirugía , Infección de la Herida Quirúrgica/microbiología , Infección de Heridas/microbiología , Anciano , Resultado Fatal , Femenino , Humanos , Infecciones por Klebsiella/tratamiento farmacológico , Infección de la Herida Quirúrgica/tratamiento farmacológico , Infección de Heridas/tratamiento farmacológicoRESUMEN
Toxoplasmosis is a neglected disease that affects millions of individuals worldwide. Toxoplasma gondii infection is an asymptomatic disease, with lethal cases occurring mostly in HIV patients and organ transplant recipients. Nevertheless, atypical strains of T. gondii in endemic locations cause severe pathology in healthy individuals. Toxoplasmosis has no cure but it can be controlled by the proinflammatory immune response. The purinergic receptor P2X7 (P2X7) is involved in many inflammatory events and has been associated with genes that confer resistance against toxoplasmosis in humans. In vitro studies have reported parasite death after P2X7-receptor activation in various cell types. To understand the contribution of P2X7 during cerebral toxoplasmosis, wild-type and P2rx7 knockout mice were infected orally with T. gondii and their pathologic profiles were analyzed. We found that all P2rx7-/- mice died 8 weeks after infection with an increased number of cysts and fewer inflammatory infiltrates in their brains. The cytokines interleukin-1ß, interleukin-12, tumor necrosis factor-α, and reactive oxygen species were absent or reduced in P2rx7-/- mice. Taken together, these data suggest that the P2X7 receptor promotes inflammatory infiltrates, proinflammatory cytokines, and reactive oxygen species production in the brain, and that P2X7 signaling mediates major events that confer resistance to cerebral toxoplasmosis.
Asunto(s)
Encéfalo/patología , Susceptibilidad a Enfermedades , Inflamación/etiología , Receptores Purinérgicos P2X7/fisiología , Toxoplasma/patogenicidad , Toxoplasmosis Cerebral/etiología , Animales , Encéfalo/metabolismo , Encéfalo/microbiología , Citocinas/metabolismo , Femenino , Inflamación/metabolismo , Inflamación/patología , Mediadores de Inflamación/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Transducción de Señal , Toxoplasmosis Cerebral/metabolismo , Toxoplasmosis Cerebral/patologíaRESUMEN
Toxoplasma gondii (T. gondii) is the protozoan parasite that causes toxoplasmosis, a potentially fatal disease to immunocompromised patients, and which affects approximately 30% of the world's population. Previously, we showed that purinergic signaling via the P2X7 receptor contributes to T. gondii elimination in macrophages, through reactive oxygen species (ROS) production and lysosome fusion with the parasitophorous vacuole. Moreover, we demonstrated that P2X7 receptor activation promotes the production of anti-parasitic pro-inflammatory cytokines during early T. gondii infection in vivo. However, the cascade of signaling events that leads to parasite elimination via P2X7 receptor activation remained to be elucidated. Here, we investigated the cellular pathways involved in T. gondii elimination triggered by P2X7 receptor signaling, during early infection in macrophages. We focused on the potential role of the inflammasome, a protein complex that can be co-activated by the P2X7 receptor, and which is involved in the host immune defense against T. gondii infection. Using peritoneal and bone marrow-derived macrophages from knockout mice deficient for inflammasome components (NLRP3-/-, Caspase-1/11-/-, Caspase-11-/-), we show that the control of T. gondii infection via P2X7 receptor activation by extracellular ATP (eATP) depends on the canonical inflammasome effector caspase-1, but not on caspase-11 (a non-canonical inflammasome effector). Parasite elimination via P2X7 receptor and inflammasome activation was also dependent on ROS generation and pannexin-1 channel. Treatment with eATP increased IL-1ß secretion from infected macrophages, and this effect was dependent on the canonical NLRP3 inflammasome. Finally, treatment with recombinant IL-1ß promoted parasite elimination via mitochondrial ROS generation (as assessed using Mito-TEMPO). Together, our results support a model where P2X7 receptor activation by eATP inhibits T. gondii growth in macrophages by triggering NADPH-oxidase-dependent ROS production, and also by activating a canonical NLRP3 inflammasome, which increases IL-1ß production (via caspase-1 activity), leading to mitochondrial ROS generation.
RESUMEN
Activation of the purinergic P2X7 receptor by extracellular ATP (eATP) potentiates proinflammatory responses during infections by intracellular pathogens. Extracellular ATP triggers an antimicrobial response in macrophages infected with Toxoplasma gondii in vitro, suggesting that purinergic signaling may stimulate host defense mechanisms against toxoplasmosis. Here, we provide in vivo evidence in support of this hypothesis, by showing that P2X7-/- mice are more susceptible than P2X7+/+ mice to acute infection by the RH strain of T. gondii, and that this phenomenon is associated with a deficient proinflammatory response. Four days post-infection, peritoneal washes from infected P2X7-/- mice had no or little increase in the levels of the proinflammatory cytokines IL-12, IL-1ß, IFN-γ, and TNF-α, whose levels increased markedly in samples from infected P2X7+/+ mice. Infected P2X7-/- mice displayed an increase in organ weight and histological alterations in some of the 'shock organs' in toxoplasmosis - the liver, spleen and mesenteric lymph nodes. The liver of infected P2X7-/- mice had smaller granulomas, but increased parasite load/granuloma. Our results confirm that the P2X7 receptor is involved in containing T. gondii spread in vivo, by stimulating inflammation.
Asunto(s)
Receptores Purinérgicos P2X7/metabolismo , Toxoplasma/inmunología , Toxoplasmosis/inmunología , Toxoplasmosis/metabolismo , Animales , Biopsia , Citocinas/metabolismo , Femenino , Inflamación/inmunología , Inflamación/metabolismo , Inflamación/parasitología , Mediadores de Inflamación/metabolismo , Hígado/inmunología , Hígado/metabolismo , Hígado/patología , Ganglios Linfáticos/inmunología , Ganglios Linfáticos/metabolismo , Ganglios Linfáticos/patología , Masculino , Ratones , Ratones Noqueados , Carga de Parásitos , Bazo/inmunología , Bazo/metabolismo , Bazo/patología , Toxoplasmosis/mortalidad , Toxoplasmosis/parasitología , Toxoplasmosis AnimalRESUMEN
Infection by the protozoan parasite Toxoplasma gondii is highly prevalent worldwide and may have serious clinical manifestations in immunocompromised patients. T. gondii is an obligate intracellular parasite that infects almost any cell type in mammalian hosts, including immune cells. The immune cells express purinergic P2 receptors in their membrane--subdivided into P2Y and P2X subfamilies--whose activation is important for infection control. Here, we examined the effect of treatment with UTP and UDP in mouse peritoneal macrophages infected with T. gondii tachyzoites. Treatment with these nucleotides reduced parasitic load by 90%, but did not increase the levels of the inflammatory mediators NO and ROS, nor did it modulate host cell death by apoptosis or necrosis. On the other hand, UTP and UDP treatments induced early egress of tachyzoites from infected macrophages, in a Ca2+-dependent manner, as shown by scanning electron microscopy analysis, and videomicroscopy. In subsequent infections, prematurely egressed parasites had reduced infectivity, and could neither replicate nor inhibit the fusion of lysosomes to the parasitophorous vacuole. The use of selective agonists and antagonists of the receptor subtypes P2Y2 and P2Y4 and P2Y6 showed that premature parasite egress may be mediated by the activation of these receptor subtypes. Our results suggest that the activity of P2Y host cell receptors controls T. gondii infection in macrophages, highlighting the importance of pyrimidinergic signaling for innate immune system response against infection. Finally the P2Y receptors should be considered as new target for the development of drugs against T. gondii infection.
Asunto(s)
Macrófagos Peritoneales/parasitología , Macrófagos/parasitología , Receptores Purinérgicos P2Y/metabolismo , Toxoplasma/fisiología , Animales , Apoptosis/efectos de los fármacos , Células Cultivadas , Femenino , Interacciones Huésped-Parásitos/efectos de los fármacos , Macrófagos/metabolismo , Macrófagos/ultraestructura , Macrófagos Peritoneales/metabolismo , Macrófagos Peritoneales/ultraestructura , Masculino , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Microscopía Electrónica de Rastreo , Microscopía Electrónica de Transmisión , Microscopía Fluorescente , Óxido Nítrico/metabolismo , Agonistas del Receptor Purinérgico P2Y/farmacología , Antagonistas del Receptor Purinérgico P2Y/farmacología , Especies Reactivas de Oxígeno/metabolismo , Suramina/farmacología , Uridina Difosfato/farmacología , Uridina Trifosfato/farmacologíaRESUMEN
We propose an innovative product based on the nanoencapsulation of pyrimethamine (PYR), aiming an improvement of drug efficacy for the treatment of toxoplasmosis. The in vitro cytotoxicity effect of encapsulated PYR and PYR-colloidal suspension was concomitantly evaluated against LLC-MK2 lineage and mouse peritoneal macrophage showing that the cells had similar tolerance for both PYR encapsulated or in the aqueous suspension. CF1 mice acutely infected with tachyzoites of Toxoplasma gondii RH strain treated with different doses (5.0-10 mg/kg/day) of PYR-nanocapsules had survival rate higher than the animals treated with the same doses of non-encapsulated PYR. Thus, encapsulation of PYR improved the efficacy of this drug against an acute model of toxoplasmosis in mice and can be considered an alternative for reducing the dose of PYR, which, in turn, could also reduce the side effects associated to the treatment.
Asunto(s)
Coccidiostáticos/administración & dosificación , Pirimetamina/administración & dosificación , Toxoplasma/efectos de los fármacos , Toxoplasmosis Animal/tratamiento farmacológico , Animales , Línea Celular , Supervivencia Celular/efectos de los fármacos , Coccidiostáticos/uso terapéutico , Coccidiostáticos/toxicidad , Femenino , Lípidos , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/fisiología , Ratones , Nanocápsulas , Pirimetamina/uso terapéutico , Pirimetamina/toxicidad , Toxoplasmosis Animal/parasitologíaRESUMEN
Toxoplasmosis is caused by the protozoan parasite Toxoplasma gondii, which is widespread throughout the world. After active penetration, the parasite is enclosed within a parasitophorous vacuole and survives in the host cell by avoiding, among other mechanisms, lysosomal degradation. A large number of studies have demonstrated the importance of ATP signalling via the P2X(7) receptor, as a component of the inflammatory response against intracellular pathogens. Here we evaluate the effects of extracellular ATP on T. gondii infection of macrophages. ATP treatment inhibits the parasite load and the appearance of large vacuoles in the cytoplasm of intracellular parasites. ROS and NO assays showed that only ROS production is involved with the ATP effects. Immunofluorescence showed colocalization of Lamp1 and SAG1 only after ATP treatment, suggesting the formation of phagolysosomes. The involvement of P2X(7) receptors in T. gondii clearance was confirmed by the use of P2X(7) agonists and antagonists, and by infecting macrophages from P2X(7) receptor-deficient mice. We conclude that parasite elimination might occur following P2X(7) signalling and that novel therapies against intracellular pathogens could take advantage of activation of purinergic signalling.
Asunto(s)
Macrófagos Peritoneales/metabolismo , Macrófagos Peritoneales/parasitología , Receptores Purinérgicos P2/metabolismo , Toxoplasma/fisiología , Adenosina Trifosfato/análogos & derivados , Adenosina Trifosfato/metabolismo , Animales , Antígenos de Protozoos/metabolismo , Femenino , Inmunohistoquímica , Estadios del Ciclo de Vida/fisiología , Proteínas de Membrana de los Lisosomas/metabolismo , Macrófagos Peritoneales/inmunología , Masculino , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Óxido Nítrico/metabolismo , Proteínas Protozoarias/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Receptores Purinérgicos P2/inmunología , Receptores Purinérgicos P2X7 , Transducción de Señal , Toxoplasma/inmunología , Toxoplasma/metabolismo , Toxoplasmosis/inmunología , Toxoplasmosis/parasitología , Vacuolas/metabolismo , Vacuolas/parasitologíaRESUMEN
Objetivos: fazer uma revisão dos aspectos básicos da ultraestrutura do taquizoíto de Toxoplasma gondii, agente etiológico da toxoplasmose. Fonte de dados: os dados apresentados tomam como referência resultados recentes obtidos pelos principais grupos de pesquisadores no mundo, que se dedicam ao estudo do Toxoplasma gondii, incluindo-se dados do próprio grupo de autores. Síntese dos dados: os taquizoítos de Toxoplasma gondii são responsáveis pela fase aguda da infecção, penetrando ativamente, através do complexo apical, em células dos hospedeiros onde se multiplicam. São abordadas características ultraestruturais e moleculares particulares da película, do citoesqueleto, de organelas secretórias (róptrias, micronemas e grânulos densos) e não secretórias (apicoplasto) exclusivas do filo Apicomplexa, além das peculiaridades do núcleo, mitocôndria, acidocalcisomas, retículo endoplasmático e complexo de Golgi desses parasitos intracelulares. Conclusões: estas características confirmam que o sucesso nas etapas de adesão, invasão e multiplicação do parasito possui clara correlação com suas características morfofuncionais.
Aims: To review basic aspects on the ultrastructure of the tachyzoite of Toxoplasma gondii, the causative agent of toxoplasmosis. Source of data: The data presented are based on recent publications by the most distinguished research groups in the area dedicated to the study of Toxoplasma gondii, including studies from the present authors. Summary of findings: The tachyzoites are responsible for the acute phase of the infection by actively penetrating, through the parasites? apical complex, the host cells where they multiply. Both ultrastructural and molecular particularities of the pellicle, the cytoskeleton, secretory (rhoptries, micronemas and dense granules) and non secretory (apicoplast) organelles, specific to Apicomplexa phylum, besides peculiar features of the nucleus, mitochondrion, acidocalcisomes, endoplasmic reticulum and Golgi complex of these intracellular parasites. Conclusions: These characteristics confirm that the success in the process of adhesion, invasion and multiplication of this parasite is clearly correlated to its morphology.
Asunto(s)
Anticuerpos Antiprotozoarios , Antígenos de Protozoos , Apicomplexa , Reacción de Fase Aguda , Toxoplasma/ultraestructura , ToxoplasmosisRESUMEN
The Bacteroides fragilis ATCC strain was grown in a synthetic media with contrasting redox potential (Eh) levels [reduced (-60 mV) or oxidised (+100 mV)] and their adhesion capacity to extracellular matrix components was evaluated. The strain was capable of adhering to laminin, fibronectin, fibronectin + heparan sulphate and heparan sulphate. A stronger adherence to laminin after growing the strain under oxidising conditions was verified. Electron microscopy using ruthenium red showed a heterogeneous population under this condition. Dot-blotting analyses confirmed stronger laminin recognition by outer membrane proteins of cells cultured at a higher Eh. Using a laminin affinity column, several putative laminin binding proteins obtained from the cultures kept under oxidising (60 kDa, 36 kDa, 25 kDa and 15 kDa) and reducing (60 kDa) conditions could be detected. Our results show that the expression of B. fragilis surface components that recognise laminin are influenced by Eh variations.
Asunto(s)
Adhesión Bacteriana , Bacteroides fragilis/crecimiento & desarrollo , Proteínas Portadoras/metabolismo , Laminina/metabolismo , Anticuerpos Monoclonales , Proteínas de la Membrana Bacteriana Externa/metabolismo , Bacteroides fragilis/metabolismo , Bacteroides fragilis/ultraestructura , Matriz Extracelular/metabolismo , Immunoblotting , Microscopía Electrónica de Transmisión , Oxidación-Reducción , Polisacáridos Bacterianos/metabolismo , Factores de TiempoRESUMEN
The Bacteroides fragilis ATCC strain was grown in a synthetic media with contrasting redox potential (Eh) levels [reduced (-60 mV) or oxidised (+100mV)] and their adhesion capacity to extracellular matrix components was evaluated. The strain was capable of adhering to laminin, fibronectin, fibronectin + heparan sulphate and heparan sulphate. A stronger adherence to laminin after growing the strain under oxidising conditions was verified. Electron microscopy using ruthenium red showed a heterogeneous population under this condition. Dot-blotting analyses confirmed stronger laminin recognition by outer membrane proteins of cells cultured at a higher Eh. Using a laminin affinity column, several putative laminin binding proteins obtained from the cultures kept under oxidising (60 kDa, 36 kDa, 25 kDa and 15 kDa) and reducing (60 kDa) conditions could be detected. Our results show that the expression of B. fragilis surface components that recognise laminin are influenced by Eh variations.
Asunto(s)
Adhesión Bacteriana , Bacteroides fragilis/crecimiento & desarrollo , Proteínas Portadoras/metabolismo , Laminina/metabolismo , Anticuerpos Monoclonales , Proteínas de la Membrana Bacteriana Externa/metabolismo , Bacteroides fragilis/metabolismo , Bacteroides fragilis/ultraestructura , Matriz Extracelular/metabolismo , Immunoblotting , Microscopía Electrónica de Transmisión , Oxidación-Reducción , Polisacáridos Bacterianos/metabolismo , Factores de TiempoRESUMEN
The antifungal agent itraconazole is an effective drug against systemic mycoses inhibiting cytochrome P-450-mediated ergosterol synthesis, essential for fungal survival. In this work, we show the activity of this azole as a potential agent against Toxoplasma gondii, the causative agent of toxoplasmosis. Monolayers of LLC-MK2 epithelial cells infected with tachyzoites of RH strain were incubated with different concentrations of itraconazole for 24 and 48 h. The IC(50) values obtained were 114.0 and 53.6 nM for 24 and 48 h, respectively. Transmission electron microscopy (TEM) analysis of itraconazole-treated intracellular tachyzoites showed endoplasmic reticulum and nuclear envelope swelling. The drug also caused rupture of the parasite's surface membrane and affected the parasite's division by endodyogeny. This observation was confirmed both by fluorescence microscopy of cells labeled with diamidino-2-phenylindole and by three-dimensional reconstruction of serial thin sections analyzed by TEM. The treatment with itraconazole led to the formation of a mass of daughter cells, suggesting the interruption of the scission process during the parasite's cell division.
Asunto(s)
Células Epiteliales/parasitología , Itraconazol/farmacología , Toxoplasma/efectos de los fármacos , Animales , Itraconazol/uso terapéutico , Microscopía Electrónica de Transmisión , Toxoplasma/crecimiento & desarrollo , Toxoplasma/metabolismo , Toxoplasmosis/tratamiento farmacológicoRESUMEN
Toxoplasma gondii resides in a nonfusogenic parasitophorous vacuole (PV), which provides a safe environment for parasite survival and replication. In this work, we used the freeze-fracture technique to analyze the PV during different times of T. gondii infection in an epithelial cell line. After a short time of interaction with host cell, T. gondii PV membrane already showed a significant quantity of intramembranous particles (IMPs)-293IMPs/microm(2). The IMP density evaluated did not vary until 6h of interaction. As the PV area enlarged with the progression of infection, the density of these particles increased, reaching a stable quantity in the order of 1100particles/microm(2). The IMPs were heterogeneous in size and were found distributed without any special pattern throughout the time of infection studied. The membrane lining the PV presented circular figures, which resembled vesicle fusion areas or attachments of the membranous tubular network, regions free from particles and small depressions, demonstrating to be a dynamic structure. IMPs were found in tubulo-vesicular structures present in the intravacuolar matrix, although rarely observed in elements of the intravacuolar network.