[Application of lytic enzymes for disruption of microbial cell walls to isolate DNA]. / Primenenie preparata liticheskikh fermentov dlia razrusheniia kletochiykh stenok mikroorganizmov s-tsel'iu vydeleniia iz nikh DNK.

The possibility of isolating DNA from yeast and actinomycete cells, previously exposed to enzymic disruption, was explored. The enzyme was produced from Actinomyces griseinus II. The highest yield of DNA from microbial cells was obtained as a result of detergent pretreatment of the yeast and actinomycetes and subsequent disruption of cell walls with the above enzyme.

[Study of lytic enzymes and prospects of their use]. / Issledovanie liticheskikh fermentov i perspektiby ikh ispol'zovaniia.

The paper reviews different procedures of preparation of lytic enzymes of microbiol origin, their properties and areas of application. It discusses the capacity of different microorganisms, actinomycetes and bacteria including (for instance, Actinomyces griseinus 11 and Bacillus subtilis 12), to produce lytic enzymes. The paper describes the conditions of disruption of yeast cells by lytic enzymes and demonstrates experimentally possible preparation of yeast lysates and protein isolates that can be used as food products.

[Thermogenesis of mesophilic, thermotolerant and thermophilic strains of microorganisms--producers of fungal cell wall--destroying enzymes]. / Termogenez mezofil'nogo, termotolerantnogo i termofil'nogo shtammov mikroorganizmov--produtsentov fermentov, razrushaiushchikh kletochnye stenki drozhzhei

Investigations were carried out to clarify the relationship between thermogenesis and production of yeast wall lyzing enzymes by the mesophilic strain of Bacillus subtilis, thermotolerant strain of Actinomyces sp. II and thermophilic strain of Actinomyces sp. 10. The enzymic lyzing activity was measured in the culture liquid filtrate of those microorganisms. The thermophilic strain of Actinomyces sp. 10 showed the highest enzymic activity. The thermogenetic curves of the cultures had several inflections. The mesophilic culture of Bacillus subtilis whose enzymic lyzing activity was the lowest displayed the highest heat release.

[Enzynatic method of disrupting yeast cell walls]. / Fermentativnyi metod razrusheniia kletochnykh stenok drozhzhei

The influence of the preparation of mucolytic enzymes obtained from the culture liquid filtrate of Actinomyces griseinus on the yeast Candida guilliermondii was investigated. Lipids, hydrocarbons and proteins were extracted and free amino acids were separated from the biomass made of disrupted yeast cells. The extract contained 73% of cytoplasmic substances whereas normal yeast (enzymically untreated) contain on the average 13.7%. Lipids and hydrocarbons were measured.

[Study of the yeast dissolving enzymic complex by isoelectric focusing]. / Issledovanie fermentnogo kompleksa, rastvoriaiushychego kletki drozhzhei, metodom izolektricheskogo fokusirovaniia

By isoelectric focussing the lyzing complex produced by Actinomyces griseinus-11 has been fractionated. The sole neutral protease which is active at pH 7.0 does not participate in lysis induced by other enzymes. The lyzing activity of this complex is associated with the carbohydrase enzymes that are at least three in number. Various carbohydrases may exert a synergistic effect upon their combined action on the protein-vitamin concentrate.

[Synthesis of mucolytic enzymes by thermophilic strains of microorganisms]. / Sintez mukoliticheskikh fermentov termofil'nymi shtammami mikroorganizmov

The paper presents data on the accumulation of biomass and the rate of enzyme synthesis on nutrient media of different composition by two thermophilic microorganisms isolated from soil--Bacterium sp. 1641 and Actinomyces sp. 10.