An Investigation on Internal Material Loads and Modifications in Precision Turning of Steel 42CrMo4.

The functional properties of a workpiece are determined by a modification of the surface and subsurface materials. In this work, the correlation between thermo-mechanical material loads and the modification of the residual stresses is presented. While the resulting residual stresses were measured by X-ray diffraction after machining experiments, the material loads were determined using a process simulation. The experimental data (measured process forces and results from previous experiments) are used to validate the simulation, which is then applied to calculate the internal thermo-mechanical loads of the maximal temperature and the equivalent von-Mises-stresses per volume element during the machining experiments. In conclusion, a higher depth impact of mechanical loads compared to a lower depth impact of thermal loads in precision machining is observed. For the sake of novelty, the thermo-mechanical loads were plotted and interpreted in a three-dimensional fashion. Finally, cross sections of this mutual representation at certain constant material loads-thermal and mechanical-result in a process signature, which can prospectively improve the prediction of functional workpiece properties.

An Investigation on High-Resolution Temperature Measurement in Precision Fly-Cutting.

The loads acting on a workpiece during machining processes determine the modification of the surface of the final workpiece and, thus, its functional properties. In this work, a method that uses thermocouples to measure the temperature in precision fly-cutting machining with high spatial and temporal resolution is presented. Experiments were conducted for various materials and machining parameters. We compare experimental measurement data with results from modern and advanced machining process simulation and find a good match between experimental and simulation results. Therefore, the simulation is validated by experimental data and can be used to calculate realistic internal loads of machining processes.

Molecular mechanisms of the non-coenzyme action of thiamin in brain: biochemical, structural and pathway analysis.

Thiamin (vitamin B1) is a pharmacological agent boosting central metabolism through the action of the coenzyme thiamin diphosphate (ThDP). However, positive effects, including improved cognition, of high thiamin doses in neurodegeneration may be observed without increased ThDP or ThDP-dependent enzymes in brain. Here, we determine protein partners and metabolic pathways where thiamin acts beyond its coenzyme role. Malate dehydrogenase, glutamate dehydrogenase and pyridoxal kinase were identified as abundant proteins binding to thiamin- or thiazolium-modified sorbents. Kinetic studies, supported by structural analysis, revealed allosteric regulation of these proteins by thiamin and/or its derivatives. Thiamin triphosphate and adenylated thiamin triphosphate activate glutamate dehydrogenase. Thiamin and ThDP regulate malate dehydrogenase isoforms and pyridoxal kinase. Thiamin regulation of enzymes related to malate-aspartate shuttle may impact on malate/citrate exchange, responsible for exporting acetyl residues from mitochondria. Indeed, bioinformatic analyses found an association between thiamin- and thiazolium-binding proteins and the term acetylation. Our interdisciplinary study shows that thiamin is not only a coenzyme for acetyl-CoA production, but also an allosteric regulator of acetyl-CoA metabolism including regulatory acetylation of proteins and acetylcholine biosynthesis. Moreover, thiamin action in neurodegeneration may also involve neurodegeneration-related 14-3-3, DJ-1 and ß-amyloid precursor proteins identified among the thiamin- and/or thiazolium-binding proteins.

Highly selective reactions of C60Cl6 with thiols for the synthesis of functionalized [60]fullerene derivatives.

Chlorofullerene C(60)Cl(6) undergoes highly selective reactions with thiols forming compounds C(60)[SR](5)H with high yields. These reactions open up straightforward synthetic routes to many functionalized fullerene derivatives, e.g. water-soluble compounds showing interesting biological activities.