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Anal Bioanal Chem ; 408(22): 6045-52, 2016 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-27481170

RESUMEN

This research outlines the application of an enzyme-linked immunosorbent assay (ELISA) for the analysis of clenbuterol in animal products. Our assay showed good sensitivity for clenbuterol (0.4 ng/g or 0.4 ppb) and low detection limit (0.09 ng/g or 0.09 ppb). A low cross-reactivity for other ß2-agonist drugs such as salbutamol, terbutaline, and epinephrine led to formatting an ELISA kit considered to have a high specificity for clenbuterol. A survey of Ho Chi Minh City pork market was conducted as part of the validation of our ELISA. ELISA results showed a surprisingly high value of contamination. However, it will be necessary to conduct a more statistically valid replicated survey with evaluation by other instrumental methods to obtain a definite conclusion. This ELISA kit will be used to monitor growth promoter residues in Vietnam's animal products.


Asunto(s)
Agonistas Adrenérgicos beta/análisis , Clenbuterol/análisis , Ensayo de Inmunoadsorción Enzimática/métodos , Contaminación de Alimentos/análisis , Haptenos/química , Carne Roja/análisis , Agonistas Adrenérgicos beta/inmunología , Animales , Formación de Anticuerpos , Clenbuterol/inmunología , Femenino , Haptenos/inmunología , Límite de Detección , Conejos , Porcinos
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