Poor outcomes of bone filling mesh container vertebroplasty for the treatment of osteoporotic vertebral compression fractures with vertebral body wall incompetence / 中国组织工程研究

BACKGROUND: Bone filling mesh container vertebroplasty can effectively correct the kyphotic deformity of the vertebral body, restore the height of the vertebral body, and effectively reduce the rate of bone cement leakage. However, there are few reports on the poor prognosis of bone filling mesh container vertebroplasty in thoracolumbar compression fractures with vertebral body wall incompetence. OBJECTIVE: To discuss adverse outcomes of bone filling mesh container vertebroplasty for the treatment of osteoporotic vertebral compression fractures with vertebral body wall incompetence. METHODS: Totally 19 cases with osteoporotic vertebral compression fracture with vertebral body wall incompetence, who were treated in the Tianjin First Central Hospital from April 2017 to October 2018, were enrolled in this study, including 3 males and 16 females, at the age of 60-86 years. The patients underwent bone filling mesh container vertebroplasty. Postoperative complications were recorded during follow up. Visual analogue scale score and Oswestry disability index were assessed. X-ray films were taken to measure the height of injured vertebral body and Cobb’s angle. This study was approved by the Ethics Committee of Clinical Research Project of Tianjin First Central Hospital (approval No. 2018N150KY). RESULTS And CONCLUSION: (1) All 19 patients were follow-up for 9-20 months. No death occurred during and after operation, and no severe complications such as pulmonary embolism, bone cement allergy or infection occurred. Among them, seven cases had poor prognosis, including five cases of bone cement leakage, four cases of above moderate pain and two cases of adjacent vertebral fractures. (2) Visual analogue scale score, Oswestry disability index, height of injured vertebral body and Cobb’s angle were significantly improved during the final follow-up in 19 patients (P < 0.05). (3) Results showed that poor outcomes of bone filling mesh container vertebroplasty for the treatment of osteoporotic vertebral compression fractures with vertebral body wall incompetence included bone cement leakage, adjacent vertebral body fracture, and postoperative pain. The operation should be carried out in strict accordance with the relevant operation specifications, and the relevant treatment and preventive measures should be made to minimize the occurrence of adverse reactions.

Impact of Kuntai Capsules on LIF, IGF-1 and EGF expression in the implantation window of endometrium in mice / Impacto de cápsulas de Kuntai en la expresión de LIF, IGF-1 y EGF en la ventana de implantación de endometrio en ratones

To investigate the influence of Kuntai capsules on the expression level of leukemia inhibitory factor (LIF), insulin-like growth factor-I (IGF-1)and epidermal growth factor (EGF) during the mouse's implantation window of superovulation period and controlled ovarian hyperstimulation period. 90 female mice were randomly divided into six groups in control, superovulation and controlled ovarian hyperstimulation (COH) conditions. The RNA expression of EGF, LIF and IGF-1 in the endometrium on the 4th day of pregnancy was detected, and the relative expression was compared. mRNA expression of these three factors in endometrium was significantly lower in superovulation and COH groups than control group (p<0.001). mRNA expression of these three factors in endometrium remained obviously lower in superovulation plus kuntai capsule group and COH plus kuntai capsule group than control group (p<0.01). mRNA expression of these three factors in endometrium was lower in control group than in the NS plus kuntai capsule group (p<0.05). Kuntai capsule cannot completely reverse the endometrial damages caused by superovulation and COH. Thus Kuntai capsule could partially improve a mouse's endometrial receptivity during the implantation window.

Para investigar la influencia de las cápsulas de Kuntai en el nivel de expresión del factor inhibidor de la leucemia (LIF), el factor de crecimiento similar a la insulina I (IGF-1) y el factor de crecimiento epidérmico (EGF) durante la ventana de implantación del ratón del período de superovulación y la hiperestimulación ovárica controlada período, se dividieron aleatoriamente 90 ratones hembra en seis grupos en condiciones de control, superovulación e hiperestimulación ovárica controlada (COH). Se detectó la expresión de ARN de EGF, LIF e IGF-1en el endometrio al cuarto día de embarazo, y se comparó la expresión relativa. La expresión de ARNm de estos tres factores en el endometrio fue significativamente menor en los grupos de superovulación y COH que en el grupo control (p<0,001). La expresión de ARNm de estos tres factores en el endometrio permaneció más baja en el grupo de cápsulas de superovulación más Kuntai y en el grupo de cápsulas de COH más Kuntai respecto del grupo control (p<0,01). La expresión de ARNm de estos tres factores en el endometrio fue menor en el grupo control que en el grupo de cápsula NS más Kuntai (p<0,05). La cápsula de Kuntai no pudo revertir completamente los daños endometriales causados por la superovulación y la COH. Por lo tanto, se sugiere que la cápsula de Kuntai podría mejorar parcialmente la receptividad endometrial de un ratón durante la ventana de implantación.

Zinc-mediated highly α-regioselective prenylation of imines with prenyl bromide.

A highly α-regioselective prenylation of imines has been successfully developed. The efficiency of this approach is confirmed by a wide range of imines including N- and C-aryl aldimines, N-alkyl aldimines, C-alkyl aldimines, and N- and C-aryl ketimines. The approach uses prenyl bromide as the prenyl source and inexpensive and convenient zinc as the mediator as well as environmentally benign 1,3-dimethyl-2-imidazolidinone (DMI) as the solvent.

Synthesis and antitumor activity of conjugates of 5-Fluorouracil and emodin.

A series of conjugates of 5-Fluorouracil (5-FU) and emodin were synthesized by coupling trimethyl emodin with N(1), N(3) dialkylated 5-FU. The 5-FU moiety contained various substituents at the N(3)-position were linked to the 2-position of trimethyl emodin via a methylene linkage. Their cytotoxicity against three cancer cell lines and one noncancerous cell were studied. The results revealed that some of conjugates exhibited better or comparable in vitro antitumor activity to 5-FU and emodin and low toxicity in the normal cell. The structure-activity relationship study showed N(3)-aromatic substituent was important for their cytotoxic activity.

General and highly α-regioselective zinc-mediated prenylation of aldehydes and ketones.

A simple, efficient, and general α-prenylation approach for the synthesis of a variety of α-prenylated alcohols has been successfully developed. A wide range of α-prenylated alcohol derivatives could be obtained in good yields by highly α-regioselective zinc-mediated prenylation of various aldehydes and ketones with prenyl bromide at 120 °C in HMPA. By simply altering the reaciton solvent and temperature, the method allows the achievement of a highly notable opposite regiocontrol, providing the expected regiochemical product. The method provides a convenient route for the direct α-prenylation of carbonyl compounds in a highly α-regioselective manner using a cheap and convenient mediator. Two possible pathways are proposed to account for the formation of these synthetically difficult-to-obtain molecules.