Potential Protective Effect of SeleniumEnriched Lactobacillus plantarum on Cadmium-Induced Liver Injury in Mice.

Cadmium (Cd) is a prevalent environmental contaminant that poses a potential hazard to the health of both humans and animals. In this study, biosynthesized selenium-enriched Lactobacillus plantarum and selenium nanoparticles (SeNPs) were developed and evaluated for their protective effects against Cd-induced hepatic injury in mice through oral administration for 4 weeks. Cadmium exposure resulted in severe impairment of liver function, as evidenced by increased levels of serum markers of liver injury and, oxidative stress and significant damage to liver tissue, and a notable decrease in the diversity of the intestinal microbiota. Oral administration of Se-enriched L. plantarum (LS) reduced cadmium accumulation in the liver by 49.5% and, restored other cadmium-induced damage markers to normal levels. A comparison of the effects with those of L. plantarum (L) and SeNPs isolated from LS revealed that LS could more effectively alleviate hepatic oxidative stress and reduce the intrahepatic inflammatory responses of the liver, further protecting against cadmiuminduced liver injury. These findings suggest that the development of LS may be effective at protecting the liver and intestinal tract from cadmium-induced damage.

Phytoremediation Competence of Composite Heavy-Metal-Contaminated Sediments by Intercropping Myriophyllum spicatum L. with Two Species of Plants.

A variety of remediation approaches have been applied to reduce the harm and diffusion of heavy metals in aquatic sediments; however, phytoremediation in co-contaminated soils is still not clear. In order to explore the phytoremediation of sediments contaminated by Cu and Pb, two submerged plants with different characteristics, Vallisneria natans and Hydrilla verticillata, were interplanted with Myriophyllum spicatum. By simulating a submerged plant ecological environment, medium-scale-simulated ecological remediation experiments were carried out. The results showed that the two planting patterns were effective in repairing the sediments in the Cu and Pb contaminated sediments. The intercropping of Myriophyllum spicatum and Vallisneria natans can be used as the plant stabilizer of Cu because of the TF > 1 and BCF < 1, and the intercropping with Hydrilla verticillata can regulate the enrichment efficiency of Myriophyllum spicatum. The removal rates of Cu and Pb in sediments reached 26.1% and 68.4%, respectively, under the two planting patterns. The risk grade of the restored sediments was RI < 150, indicating a low risk.

Seasonal and long-term trends of sulfate, nitrate, and ammonium in PM2.5 in Beijing: implication for air pollution control.

Particulate matter pollution in Beijing is a serious environmental problem. In response to this, the Beijing government has implemented comprehensive emission reduction measures in recent years. To assess the effectiveness of these measures, the seasonal and long-term trends in chemical compositions of PM2.5 in Beijing have been studied based on daily samples collected from July 2015 to April 2016 and literature investigation (2000-2014). Results show that the concentrations of sulfate, nitrate, and ammonium in PM2.5 have significant seasonal variations, which are related to the changes in meteorological conditions and emission intensities. In addition, the long-term data display that the concentrations of sulfate, nitrate, and ammonium have significantly decreased between 2013 and 2016, which are consistent with the reduction in PM2.5 levels (~ 11.2 µg/m3 per year). The declines could not be interpreted by the meteorological factors. It suggests that the air pollution control measures in Beijing (2013-2016), especially the decreasing consumption of coal, can effectively decrease the mass concentration of fine particles. To further improve the air quality, similar measures should be adopted in the areas around Beijing. These air pollution control measures taken in Beijing can provide invaluable guidance for mega-cities in China and other developing countries to decrease their PM2.5 concentration and reduce health risk from particulate pollution.

Tanshinone II A stabilizes vulnerable plaques by suppressing RAGE signaling and NF-κB activation in apolipoprotein-E-deficient mice.

Tanshinone II A (TSIIA) is a diterpene quinone extracted from the roots of Salvia miltiorrhiza with anti-inflammatory and anti­oxidant properties that is used to treat atherosclerosis. In the current study, morphological analyses were conducted to evaluate the effects of TSIIA on atherosclerotic vulnerable plaque stability. Additionally, receptor of advanced glycation end products (RAGE), adhesion molecule, and matrix­metalloproteinases (MMPs) expression, and nuclear factor-κB (NF­κB) activation were examined in apolipoprotein E (apoE)­deficient mice treated with TSIIA. Eight­week­old apoE-/- mice were administered TSIIA and fed an atherogenic diet for 8 weeks. TSIIA exhibited no effects on plaque size. Analysis of the vulnerable plaque composition demonstrated decreased numbers of macrophages and smooth muscle cells, and increased collagen content in apoE­deficient mice treated with TSIIA compared with untreated mice. Western blotting revealed that TSIIA downregulated the expression levels of vascular cellular adhesion molecule-1 (VCAM-1), intercellular adhesion molecule­1 (ICAM­1), and MMP­2, ­3, and ­9, suppressed RAGE, and inhibited NF­κB, JNK and p38 activation. The present study demonstrated that the underlying mechanism of TSIIA stabilization of vulnerable plaques involves interfering with RAGE and NF­κB activation, and downregulation of downstream inflammatory factors, including ICAM­1, VCAM­1, and MMP­2, ­3 and ­9 in apoE-/- mice.

Fractionation of Stable Cadmium Isotopes in the Cadmium Tolerant Ricinus communis and Hyperaccumulator Solanum nigrum.

Cadmium (Cd) isotopes provide new insights into Cd uptake, transport and storage mechanisms in plants. Therefore, the present study adopted the Cd-tolerant Ricinus communis and Cd-hyperaccumulator Solanum nigrum, which were cultured under controlled conditions in a nutrient solution with variable Cd supply, to test the isotopic fractionation of Cd during plant uptake. The Cd isotope compositions of nutrient solutions and organs of the plants were measured by multiple collector inductively coupled plasma mass spectrometry (MC-ICPMS). The mass balance of Cd isotope yields isotope fractionations between plant and Cd source (δ(114/110)Cdorgans-solution) of -0.70‰ to -0.22‰ in Ricinus communis and -0.51‰ to -0.33‰ in Solanum nigrum. Moreover, Cd isotope fractionation during Cd transport from stem to leaf differs between the Cd-tolerant and -hyperaccumulator species. Based on these results, the processes (diffusion, adsorption, uptake or complexation), which may induce Cd isotope fractionation in plants, have been discussed. Overall, the present study indicates potential applications of Cd isotopes for investigating plant physiology.

Cadmium accumulation and tolerance of two castor cultivars in relation to antioxidant systems.

To investigate the effects of Cd on tolerance and antioxidant activities of castor, two different castor (Ricinus communis L.) cultivars (Zibo No. 5 and Zibo No. 8) were used for a hydroponic experiment (0, 1 and 2mg/L Cd) and a pot experiment using Cd-contaminated soil (34mg/kg) with the addition of ethylenedinitrilotetraacetic acid (EDTA). The results indicated that there were significant differences between the two cultivars with respect to Cd uptake in shoots (113-248mg/kg for Zibo No. 5 and 130-288mg/kg Zibo No. 8), biomass tolerance indexes (64.9%-74.6% for Zibo No. 5 and 80.1%-90.9% for Zibo No. 8) in the hydroponic experiment and survival rates (0% for Zibo No. 5 and 100% for Zibo No. 8) determined by the addition of EDTA in the pot experiment, suggesting that Zibo No. 8 has higher tolerance than Zibo No. 5. Moreover, the castor cultivars have low bioconcentration factors (4.80% for Zibo No. 5 and 5.43% for Zibo No. 8) and low translocation factors (<1%). Consequently, Zibo No. 8 can participate in Cd phytostabilization in highly Cd-polluted areas. The results indicated that glutathione (GSH) as a non-enzymatic antioxidant, and antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT) and guaiacol peroxidase (GPX), were cultivar- and dose-dependent. The higher tolerance of Zibo No. 8 compared with Zibo No. 5 can be attributed to the higher GSH levels in the root and higher GPX activity in the leaf.

[Research on relationship between angiotensinogen gene M235T and TCM syndrome type in essential hypertension patients].

OBJECTIVE: To explore the relationship between angiotensinogen (AGT) gene M235T and TCM syndrome type in essential hypertension (ET) patients. METHODS: The gene mutation frequency of AGT M235T in 168 ET patients and 42 nomotensive (NT) subjects were determined using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. RESULTS: There was a significant difference in AGT M235T gene mutation between patients of Gan-fire exuberant type and those of yin-yang deficiency type (P < 0.01), homozygote type TT appeared with higher frequency. Multivariate regression linear analysis demonstrated that the genotypes of AGT M235T was correlated with the prognosis of ET to a certain degree. CONCLUSION: Gene mutation of AGT M235T may be associated with the genesis and development of ET, and the TCM syndrome type of ET has its own intrinsic molecular biological background.