RESUMEN
OBJECTIVE: To explore the relationship between HLA-A, -B, -C, -DRB1, -DQB1 gene polymorphism and aplastic anemia ï¼AAï¼of 65 cases in Northern China. METHODS: The high resolution genotyping of HLA-A, -B, -C, -DRB1, -DQB1 alleles in 65 AA patients and 772 healthy controls was performed with polymerase chain reaction-sequence specific oligonucleotide (PCR-SSO), the relationship between HLA-A, -B, -C, -DRB1, -DQB1 gene polymorphism and aplastic anemia was analyzed by Pearson Chi-squareï¼Continuity Correctionï¼ Two-sided Fisher's Exact Test and Odds Ratio. RESULTS: The HLA-B*1302ï¼10% vs 4.21%ï¼, B*3501ï¼7.69% vs 3.89%ï¼, DRB1* 0701ï¼10% vs 4.73%ï¼, DRB1*0901ï¼19.23% vs 7.58%ï¼, DQB1*0202ï¼9.23% vs 3.76%ï¼ gene frequency in AA patients was higher than those in health controls, the difference was statistically significant (P<0.05), the χ2 were 9.049, 4.336, 6.838, 20.974 and 8.968, OR ratio was 2.528, 2.061, 2.239, 2.904 and 2.605. However, the HLA-A*3303ï¼1.54% vs 6.93%ï¼, DQB1*0302ï¼1.54% vs 6.02%ï¼ gene frequency in AA patients was lower than those in healthy controls, the difference was statistically significant (P<0.05), the χ2 was 5.726 and 4.505, the OR ratio were 0.210 and 0.244. CONCLUSION: The polymorphism of HLA-A, -B, -DRB1, -DQB1 alleles is associate with AA in these patient cases, the HLA-B*1302, HLA-B*3501, HLA-DRB1*0701, HLA-DRB1*0901 and HLA-DQB1*0202 may be sensitive genes to AA, while the HLA-A*3303 and HLA-DQB1*0302 may be protective genes on AA.
