RESUMEN
One hundred and one imported falciparum malaria cases in Wuhan City were confirmed by microscopy and Nest-PCR, and the blood samples were collected. The Pf60.1 gene was amplified by PCR. Among 101 blood samples, three Pf60.1 fragments [313 bp (56.5%, 52/92), 340 bp (37.0%, 34/92), 313 bp+340 bp (6.5%, 6/92)] were amplified from 92 samples. Among 83 blood samples from patients returning from Africa, 313 bp fragment were found in 46 samples (55.4%, 46/83), 340 bp fragment were found in 31 samples (37.1%, 31/83), and 7.2% (6/83) was mixed-fragment (313 bp+340 bp). Among 9 samples from southeast Asia, 6 samples were with 313 bp fragment and 3 samples with 340 bp fragment. The results indicated that the most common genotype was 313 bp-genotype, and there would be polyclonal P. falciparum infections.
Asunto(s)
Malaria Falciparum/parasitología , Plasmodium falciparum/genética , Polimorfismo Genético , Proteínas Protozoarias/genética , Humanos , Microscopía , Reacción en Cadena de la PolimerasaRESUMEN
The process of diagnosis, epidemiological survey and treatment of two cases of African imported quartan malaria is reported in this paper.
Asunto(s)
Malaria/diagnóstico , Malaria/tratamiento farmacológico , Plasmodium malariae/fisiología , África , Humanos , Malaria/sangre , Malaria/transmisión , Masculino , Persona de Mediana Edad , Plasmodium malariae/genética , Plasmodium malariae/aislamiento & purificación , ViajeRESUMEN
OBJECTIVE: To identify the incidence of the K76T mutation in Pfcrt genes of imported Plasmodium falciparum. METHODS: The blood samples were collected from returnees infected with P. falciparum in endemic areas of Southeast Asia and Africa from 2008 to 2012. According to the Pfcrt gene sequence of P. falciparum, nested PCR primers were designed. Nested PCR-RFLP was applied with falciparum DNA in the blood samples as templates. RESULTS: Among 92 blood samples of P. falciparum, the mutant Pfcrt alleles were found in 50 samples (54.3%), and the wild type Pfcrt alleles were found in 42 samples (45.7%). There were 33 samples (47.1%) with mutant Pfcrt alleles and 37 samples (52.9%) with wild type, respectively, from Africa. There were 17 samples (77.3%) with mutant Pfcrt alleles and 5 samples (22.7%) with wild type, respectively, from Southeast Asia. There was a significant difference between the Africa group and the Southeast Asia group (Chi(2) = 6.12, P < 0.05). CONCLUSION: The incidence of Pfcrt gene mutation is different among P. falciparum isolates from different regions. Therefore, Pfcrt K76T has an application value in the surveillance of the imported falciparum chloroquine-resistance.
Asunto(s)
Malaria Falciparum/parasitología , Proteínas de Transporte de Membrana/genética , Mutación Missense , Plasmodium falciparum/genética , Polimorfismo Genético , Proteínas Protozoarias/genética , Humanos , Malaria Falciparum/transmisión , Plasmodium falciparum/aislamiento & purificación , ViajeRESUMEN
OBJECTIVE: To identify the incidence of the K76T mutation in Pfcrt gene of imported Plasmodium falciparum and study the Pfcrt gene polymorphism in Plasmodium falciparum. METHODS: Seventy-two blood samples were collected from patients infected with P. falciparum returning from Africa (Nigeria, Equatorial Guinea, Congo, Liberia, Angora and Mali) and Southeast Asia (Myanmar and Indonesia) from 2008 to 2012. According to Pfcrt gene sequence of P. falciparum, nested PCR primers were designed, and the reaction was applied with P. falciparum DNA in the blood samples as templates. PCR products were identified by Apo I digestion. RESULTS: Among 72 blood samples of P. falciparum, mutant Pfcrt alleles were found in 41 samples (57.7%, 41/71) and wild type Pfcrt alleles were found in 30 samples (42.3%, 30/71). There were 25 samples (50%, 25/50) each with mutant Pfcrt alleles or wild type that were from Africa, while 16 samples (76.2%, 16/21) with mutant Pfcrt alleles and 5 samples (23.8%, 5/21) with wild type that were from Southeast Asia, respectively. CONCLUSION: The incidence of Pfcrt gene mutation is different in P. falciparum isolates from different regions.
Asunto(s)
Proteínas de Transporte de Membrana/genética , Plasmodium falciparum/genética , Proteínas Protozoarias/genética , ADN Protozoario/genética , ADN Protozoario/aislamiento & purificación , Humanos , Malaria Falciparum/parasitología , Plasmodium falciparum/aislamiento & purificación , Mutación Puntual , Polimorfismo GenéticoRESUMEN
OBJECTIVE: To evaluate the usefulness of nested PCR method in the diagnosis of imported malaria. METHODS: A total of 210 blood smears and blood samples on filter paper were taken from persons returned from highly malaria endemic countries. The results of both nested PCR and microscopy for 210 samples were compared. RESULTS: Among the 210 persons, 43 were hospitalized due to malaria, and positive by nested PCR test Among the rest 157 people at high risk of getting malaria, 3 were found plasmodium-positive by microscope (1.91%), and 5 were positive by nested PCR (3.18%). In four samples with discrepancy between the two methods, 1 was microscopy positive and PCR negative, and 3 were microscopy negative and PCR positive. Positive and negative coincidence rate between the two tests was 66.7% and 98.1%, respectively. The coincidence between the two methods was 97.5%. CONCLUSION: Nested PCR is useful for monitoring, identification and diagnosis of imported malaria.
