RESUMEN
Tumor cells not only show a vigorous metabolic state, but also reflect the disease progression and prognosis from their metabolites. To judge the progress and prognosis of ovarian cancer is generally based on the formation of ascites, or whether there is ascites recurrence during chemotherapy after ovarian cancer surgery. To explore the relationship between the production of ascites and ovarian cancer tissue, metabolomics was used to screen differential metabolites in this study. The significant markers leading to ascites formation and chemoresistance were screened by analyzing their correlation with the formation of ascites in ovarian cancer and the clinical indicators of patients, and then provided a theoretical basis. The results revealed that nine differential metabolites were screened out from 37 ovarian cancer tissues and their ascites, among which seven differential metabolites were screened from 22 self-paired samples. Sebacic acid and 20-COOH-leukotriene E4 were negatively correlated with the high expression of serum CA125. Carnosine was positively correlated with the high expression of serum uric acid. Hexadecanoic acid was negatively correlated with the high expression of serum γ-GGT and HBDH. 20a,22b-Dihydroxycholesterol was positively correlated with serum alkaline phosphatase and γ-GGT. In the chemotherapy-sensitive and chemotherapy-resistant ovarian cancer tissues, the differential metabolite dihydrothymine was significantly reduced in the chemotherapy-resistant group. In the ascites supernatant of the drug-resistant group, the differential metabolites, 1,25-dihydroxyvitamins D3-26, 23-lactonel and hexadecanoic acid were also significantly reduced. The results indicated that the nine differential metabolites could reflect the prognosis and the extent of liver and kidney damage in patients with ovarian cancer. Three differential metabolites with low expression in the drug-resistant group were proposed as new markers of chemotherapy efficacy in ovarian cancer patients with ascites.
RESUMEN
Changbaishan diatomite was treated under the conditions of temperature from 100 to 1330 degrees C, and examined by infrared spectroscopy applying the KBr-technique. In the range from 250 to 1500 cm(-1), the spectra of the samples at 100 to 1100 degrees C show the same three characteristic broad bands at 1100, 801 and 471 cm(-1), which are similar to amorphous silica. Besides the above three broad bands, the spectra of 1200 and 1330 degrees C treated samples exhibit three new bands at 618, 386 and 301 cm(-1), which indicate that the diatornite transforms into cristobalite. While all of the eleven samples show an asymmetric broad band at 3440 cm(-1) in the range from 3000 to 4000 cm(-1), the spectrum of 500 degrees C treated sample begins to exhibit a 3745 cm(-1) band assigning to isolated Si-OH group stretching vibration. The 3745 cm(-1) band shows the highest intensity at 900 degrees C, and disappears at 1200 degrees C. According to the absorbance of the 3745 cm(-1) band of different temperature treated samples, the H2O content in the form of Si-OH group was calculated semi-quantitatively, which indicates that the Si-OH group exists at the internal structure defects in addition to existing at the surface. The transform mechanism of diatomite into cristobalite was also discussed.