A Mendelian randomization study on the causal relationship between smoking, alcohol consumption, and the development of myopia and astigmatism.

The influence of environmental factors like smoking and alcohol on myopia and astigmatism is controversial. However, due to ethical concerns, alternative study designs are urgently needed to assess causal inference, as mandatory exposure to cigarettes and alcohol is unethical. Following comprehensive screenings, 326 single nucleotide polymorphisms (SNPs) related to myopia and astigmatism were included in the dataset. To validate the causal association between exposures such as cigarette smoking, alcohol consumption, and coffee intake, and outcomes namely astigmatism and myopia, five regression models were employed. These models encompassed MR-Egger regression, random-effects inverse-variance weighted (IVW), weighted median estimator (WME), weighted model, and simple model. The instrumental variables utilized in these analyses were the aforementioned SNPs. Apply Cochran's Q test to determine heterogeneity of SNPs; if heterogeneity exists, focus on IVW model results. The IVW model showed a 1.379-fold increase in the risk of astigmatism (OR = 1.379, 95%CI 0.822~2.313, P = 0.224) and a 0.963-fold increase in the risk of myopia (OR = 0.963, 95%CI 0.666~1.393, P = 0.841) for each unit increase in smoking. For each unit increase in coffee intake, the risk of astigmatism increased 1.610-fold (OR = 1.610, 95%CI 0.444~5.835, P = 0.469) and the risk of myopia increased 0.788-fold (OR = 0.788, 95%CI 0.340~1.824, P = 0.578). For each additional unit of alcohol consumption, the risk of astigmatism increased by 0.763-fold (OR = 0.763, 95%CI 0.380~1.530, P = 0.446), and none of the differences were statistically significant. However, for each unit of alcohol consumption, the risk of myopia increased by 1.597 times, and the difference was statistically significant (OR = 1.597, 95%CI 1.023~2.493, P = 0.039). The findings indicate that alcohol consumption is a risk factor for myopia but smoking and coffee intake do not affect its development. Additionally, there is no association between smoking, alcohol consumption, coffee intake, and the risk of astigmatism.

Prevalence of mobile phone addiction among medical students: a systematic review.

The incidence and factors related to mobile phone addiction among Chinese medical students were analyzed through meta-analysis. Chinese literature databases (such as China Knowledge Network and VIP Information Resource System) and English literature databases (such as PubMed and Web of Science) were searched for cross-sectional studies on the incidence and factors related to mobile phone addiction, and the required data were extracted. Meta-analysis was performed using a random effects model with RevMan 5.3 statistical software, and publication bias was tested with Stata 12.0. A total of 20 studies were included, including 36,365 study subjects. Among them, there were 10,597 cases of mobile phone addiction with an incidence of 29.14%. The results of the meta-analysis showed that the combined OR values (95% CI) of the factors were: gender 1.070 (1.030-1.120), residence 1.118 (1.090-1.146), school type 1.280 (1.241-1.321), mobile phone use time 1.098 (1.068-1.129), sleep quality 1.280 (1.288-1.334), self-perception of learning 0.737 (0.710-0.767), and family relationship 0.821 (0.791-0.852). The study showed that being a male student from cities and towns, being at a vocational college, excessive use of mobile phones, and poor sleep quality were the risk factors for mobile phone addiction among medical students in China. Positive self-perception of learning and family relationships were protective factors, and more related factors are still controversial and need to be further explored and confirmed.

Performance of permeable pavement systems on stormwater permeability and pollutant removal.

Permeable pavement is an effective means for stormwater runoff control and pollutant removal. However, relatively few studies have examined the characteristics of permeable brick and corresponding permeable pavement system (PPS). In this work, the permeable pavement systems consisted of surface permeable brick layer (concrete or ceramic) with structural layer (including a cement mortar layer, a permeable concrete layer, and a gravel layers) were selected as typical cases to assess their permeability and runoff pollutant removal performance by laboratory experiments. The results indicated that PPS had obvious outflow hysteresis effect. The PPS with ceramic brick layer reached the saturation flow rate earlier and showed larger outflow rate than that with concrete brick layer. Both types of PPSs had a relatively high efficiency (83.8-95.2%) in removing suspended solids (SS) in stormwater runoff mainly due to the interception and filtration of the surface brick layer, whereas the structural layer of the PPS played a vital role in the removal of total phosphorus (TP). The percentage of total nitrogen (TN) removal efficiency via ceramic brick layer accounted for via corresponding PPS was obviously larger than that of concrete brick layer. The PPS also displayed a certain chemical oxygen demand (COD) removal ability: around 14.0-27.0% for concrete type and 20.9-28.9% for ceramic type. Subsequently, a multi-objective evaluation model was implemented based on the analytic hierarchy process (AHP) method to identify the optimal scheme in relation to four indices: permeability, environmental benefit, compressive strength, and comprehensive economic cost. The results showed, insofar, the ceramic PPS is preferred with a better economic performance. Our study attempts to select optimal designs of PPS and provides insight into the permeable capacity and the efficiency of pollutant removal in PPS.

The maize SUMO conjugating enzyme ZmSCE1b protects plants from paraquat toxicity.

Paraquat (PQ) herbicide causes damage to green plant tissues by inducing the production of toxic reactive oxygen species (ROS). SUMOylation is an important post-translational modification that enables plants to defend against multiple stresses. However, it is still unknown whether the SUMOylation is involved in PQ resistance response in crops. Herein, we showed that a maize SUMO conjugating enzyme gene (ZmSCE1b) functioned in PQ resistance. The quantitative real-time PCR (qRT-PCR) analysis revealed that this gene was significantly up-regulated upon PQ exposure. The overexpression of ZmSCE1b increased the levels of SUMO conjugates and improved PQ resistance in transgenic Arabidopsis. The ZmSCE1b-transgenic plants showed lower levels of ROS and lipid peroxidation, as well as higher antioxidant enzyme activities, upon PQ exposure. Furthermore, Western blotting showed that levels of SUMOylation in these transgenic plants were significantly elevated. In addition, the abundance of transcripts of several defense-related genes was apparently up-regulated in the over-expressing lines using qRT-PCR. Collectively, our results manifested the effect of overexpression of ZmSCE1b in improving resistance to PQ, possibly by regulating the levels of SUMO conjugates, antioxidant machinery, and expression of defense genes. Findings of this study can facilitate the understanding of the regulatory mechanisms underlying the involvement of SCE-mediated SUMOylation in PQ resistance response in crop plants. Meanwhile, ZmSCE1b could be utilized for engineering PQ-resistant crops in phytoremediation.

Factors associated with contracted services of Chinese family doctors from the perspective of medical staff and consumers: a cross-sectional study.

BACKGROUND: The family doctor system has developed rapidly all over the world, and in the past few years, China has actively explored family doctor-type contracted services. This study aimed to explore the related factors of Contracted Family Doctors Services (CFDS) from the perspectives of medical staff and consumers, and to provide a stronger basis for the development and promotion of CFDS. METHODS: A combination of quantitative and qualitative methods were used in this study. A self-reported questionnaire was designed through a literature analysis, group discussions, expert consultations and a pre-investigation, and conducted among community health service providers in 12 community health service centres across four provinces of China. A total of 389 participants participated, and 320 valid questionnaires were obtained, with an effective response rate of 82.3%. A total of 36 consumers participated in in-depth interviews, and the effective rate was 100.0%. An exploratory factor analysis, a confirmatory factor analysis, inductive methods, and expert consultations were used to analyse the related factors of CFDS. RESULTS: The related factors of CFDS from the perspectives of medical staff were divided into four dimensions, with the following weighting coefficients: national government factors (31.9%), community health service agency factors (24.7%), consumer-related factors (22.6%), and contracted doctor-related factors (20.8%). The related factors of CFDS from the perspectives of consumers were divided into four dimensions, with the following frequency sequence: national government factors, contract doctor-related factors, community health service agency factors, and consumers-related factors. National government factors played an important role in CFDS from the perspectives of medical staff and consumers. CONCLUSIONS: The related factors of CFDS were the same from the perspective of medical staff and consumers, but the weight of each factor was different. The development of CFDS is inseparable from the support of policies. It is suggested that the government should strengthen the publicity of CFDS, expand the coverage, introduce personalised contract programs that meet the needs of different groups, and promote the rapid development of CFDS.

The Maize Class-I SUMO Conjugating Enzyme ZmSCE1d Is Involved in Drought Stress Response.

Post-translational modification of cellular proteins by sumoylation plays a vital role in stress responses of plants. However, the mechanisms underlying the sumoylation's involvement in stress responses in crop species remain largely unknown. Herein, a maize class-I SUMO conjugating enzyme gene (ZmSCE1d) was identified, whose expression was upregulated upon drought stress. Over-expression of ZmSCE1d in transgenic Arabidopsis plants increased SUMO conjugates and improved drought tolerance. The ZmSCE1d-transgenic plants showed higher antioxidant enzyme activities, but lower reactive oxygen species and lipid peroxidation upon drought stress. Furthermore, transcripts of several drought-responsive genes were significantly elevated, as revealed by qPCR in the transgenic lines. Taken together, these data have demonstrated that ZmSCE1d overexpression improved drought tolerance likely by regulating sumoylation levels, antioxidant capability, and drought-responsive gene expression in transgenic plants. This study may facilitate our understanding of the mechanisms underlying SCE-mediated sumoylation under drought stress and accelerate genetic improvement of crop plants tolerant to drought stress by manipulating the SUMO system.

The maize bHLH transcription factor bHLH105 confers manganese tolerance in transgenic tobacco.

Manganese (Mn) toxicity is an important limiting factor for crop production in acidic soils. The basic helix-loop-helix (bHLH) transcription factors are involved in a variety of physiological processes. However, whether the bHLHs are involved in excess Mn stress response is largely unknown. Here, we report the functional characterization of ZmbHLH105 isolated from maize (Zea mays). The transcript levels of ZmbHLH105 were higher in leaves, and were markedly up-regulated under excess Mn stress in maize. ZmbHLH105 was localized in the nucleus with transactivation activity. Ectopic expression of ZmbHLH105 enhanced Mn tolerance in Saccharomyces cerevisiae cells. ZmbHLH105-overexpressing (OE) plants showed improved excess Mn tolerance in transgenic tobacco. The stress-tolerant phenotypes of these OE tobacco lines were accompanied by increases of key antioxidant enzyme activities, but decreases of reactive oxygen species (ROS) accumulations. Importantly, the OE plants had less increases than the wild-type in toxic Mn accumulation. Moreover, the transcript levels of Mn/Fe-related transporters in the OE lines displayed remarkable decreases compared with the wild-type under Mn stress, suggesting that ZmbHLH105 reduced Mn accumulation in plants largely by repressing expression of Mn/Fe-regulated transporter genes. Taken together, these results indicate that ZmbHLH105 confers improved Mn stress tolerance possibly by regulating antioxidant machinery-mediated ROS scavenging and expression of Mn/Fe-related transporters in plants. ZmbHLH105 could be exploited for developing drought-tolerant maize varieties.

Overexpression of a maize SUMO conjugating enzyme gene (ZmSCE1e) increases Sumoylation levels and enhances salt and drought tolerance in transgenic tobacco.

As an essential regulatory process of post-translational modifications, Sumoylation has been shown to play a central role in stress responses in higher plants. However, the mechanisms underlying the involvement of the Sumoylation in stress responses in crops are largely unknown. In this study, a putative SUMO conjugating enzyme ortholog from Zea mays (ZmSCE1e) was isolated. Sequence alignments and phylogenetic analysis showed that ZmSCE1e possesses a central active domain similar to known SCE1 proteins, but is the cereal-specific isoform.The transcript levels of ZmSCE1e were markedly up-regulated by salt or drought stress. Over-expression of ZmSCE1e in tobacco plants increased levels of SUMO conjugates and enhanced their tolerances to salt and drought stresses. ZmSCE1e-transgenic plants showed higher activities of key antioxidant enzymes but lower hydrogen peroxide (H2O2) and malondialdehyde (MDA) accumulations under salt or drought stress. Furthermore, expression of several stress defense genes was significantly elevated as revealed by qPCR in the ZmSCE1e-transgenic lines. Together, these data have demonstrated that ZmSCE1e improved salt and drought tolerance likely by modulating Sumoylation levels, antioxidant capability, and stress defense gene expression in transgenic plants. This study may facilitate our understanding of the biological roles of SCE-mediated Sumoylation under stress conditions in higher plants and accelerate genetic improvement of crop plants tolerant to environmental stresses.

Cold stress improves the production of artemisinin depending on the increase in endogenous jasmonate.

Previous publications reported that the artemisinin level was increased in Artemisia annua following a night-frost period. However, the molecular mechanism was not clear. In this study, we found that exogenous jasmonate (JA) effectively enhanced the freezing tolerance of A. annua. The JA biosynthetic genes (LOX1, LOX2, allene oxide cyclase [AOC], and jasmonate resistant 1 [JAR1]) were induced by cold stress, leading to an increase in endogenous JA in cold-treated A. annua. Increased endogenous JA enhanced the expression of three JA-responsive transcription factors, ethylene response factor 1, ethylene response factor 2, and octadecanoid-responsive AP2/ERF, all of which were reported to transcriptionally activate the expression of artemisinin biosynthetic genes, such as amorpha-4,11-diene synthase (ADS), CYP71AV1, DBR2, and aldehyde dehydrogenase 1 (ALDH1). Furthermore, the expression levels of the four artemisinin biosynthetic genes were also significantly increased under cold stress. Consequently, the levels of artemisinin and related secondary metabolites, such as dihydroartemisinic acid, artemisinin B, and artemisinic acid, were increased in A. annua under cold stress. Our study points to a molecular mechanism in which the production of artemisinin is regulated by cold stress in A. annua.

Molecular cloning and characterization of the promoter of aldehyde dehydrogenase gene from Artemisia annua.

In recent years, although several related genes had been cloned and characterized, the role of aldehyde dehydrogenase 1 (ALDH1), the newly cloned gene involved in artemisinin biosynthesis pathway, is still not clear. In this study, a 2,100-bp ALDH1 promoter region fused with GUS reporter gene was stably transferred into Arabidopsis thaliana. Histochemical staining showed the methyl jasmonate (MeJA) and wounding treatment induced the GUS gene expression specifically in the trichomes of transgenic A. thaliana, consistent with the results that the expression level of ALDH1 gene was increased in the A. annua under MeJA and wounding treatments. Two RAA motifs (AP2/ERF binding site) but no W box (WRKY binding site) motif were identified in the ALDH1 promoter by the analysis through PLACE and plantCARE. Through the dual luciferase reporter assay, we revealed that both AaORA and AaERF2, rather than AaWRKY1, could activate the expression of ALDH1 promoter. Our study shed light on the in-depth understanding of the role of ALDH1 in artemisinin biosynthesis.