Workplace ostracism and employee wellbeing: A conservation of resource perspective.

Introduction: As a common phenomenon of workplace ostracism in corporate management, it is urgent to clarify how it affects employee well-being. Methods: Based on Conservation of Resource Theory, this study investigates the mechanisms of workplace ostracism on employee well-being and examines the mediating role of emotional exhaustion and the moderating role of team forgiveness climate by surveying 282 employees from 68 companies in mainland China. Results: The results show that (1) workplace ostracism negatively affects employee well-being; (2) emotional exhaustion plays a mediating role between workplace ostracism and employee well-being; (3) team forgiveness climate weakens the negative effect of workplace ostracism on emotional exhaustion and negatively moderates the indirect effect of workplace ostracism on employee well-being through emotional exhaustion. Discussion: It tries to provide theoretical basis and practical guidance for eliminating the negative effects of workplace ostracism and focusing on employee well-being.

[Isolation, Identification and Characterization of the Filamentous Microorganisms from Bulking Sludge].

To illustrate the species and characteristics of filamentous microorganisms in bulking sludge, culture method, microscopic examination and molecular biological analysis were conducted to isolate and identify the filamentous microorganisms existing in bulking sludge collected from municipal wastewater treatment plant. Filamentous microorganisms isolated by Gause's synthetic agar medium and starch agar fell into 18 genera, among which Streptomyces and Microbacterium belong to bacterial phylum Actinobacteria, all other isolates were classified into fungi. Penicillium, Cladosporium, Alternaria, Aspergillus and Trichosporon were the fungi with high occurrence frequency in culture medium. Trichosporon, Streptomyces, Penicillium and Alternaria could grow well at neutral pH or meta-acid condition. High concentration of NaCl could inhibit the growth of Trichosporon and Streptomyces, but had no obvious inhibitory effect on Penicillium and Alternaria. Except of Trichosporon, Streptomyces, Penicillium and Alternaria all could utilize saccharose, starch and cellulose. The increase of carbon source concentration could promote their growth. The results of high throughput sequencing of rDNA-ITS showed that a large number of unidentified fungi existed in bulking sludge.

Inhibition of proliferation and induction of apoptosis in RB116 retinoblastoma cells by afatinib treatment.

The present study investigates the effect of afatinib on the growth, induction of apoptosis in RB116 cells, and reduction of carcinoma growth in the mice transplanted with RB116 cells. The results from MTT assay revealed that afatinib inhibited the growth of RB116 cells in a dose-dependent manner. Proliferation of RB116 cells was reduced to 64 % on treatment with 200 µM concentration of afatinib after 48 h. Afatinib treatment of RB116 cells at 200 µM concentration induced apoptosis and necrosis in 49.7 and 9.4 %, respectively, after 48 h. In the RB116-transplanted mice, treatment with afatinib at 10-mg/kg doses for 45 days caused a significant (p < 0.005) reduction in the tumor volume compared to the control group. The tissue lysates of the mice containing RB116 transplant showed a significant decrease in the expressions of Ki67 and p53 in the afatinib treatment group after 45 days. However, the expression of caspase-3 was increased and of Bcl-2 remained unaltered on treatment with afatinib. Measurement of the body weight of afatinib-treated animals showed no reduction during the study. Thus, afatinib can be of therapeutic value for the treatment of retinoblastoma.