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The characteristics of lake dissolved organic matter (DOM) pool and lake ecosystem interact, and studying the responses between sediment DOM characteristics and lake ecosystem changes may shed light on the inherent connection between ecosystem evolution and carbon biogeochemical cycles. Lakes in cold and arid regions are sensitive to changes and accumulate large amounts of carbon as DOM, which may provide a window into more explicit relationships between ecosystem evolution and changes in sediment DOM characteristics in time dimension. However, considerable blind spots exist in the responses between the sediment DOM and ecosystem evolution on time scale and the underlying mechanisms. In this study, multiple approaches were combined to investigate the relationship between the variation trend of sediment DOM characteristics and the evolution of fragile lake ecosystems across three different lake ecosystems in cold and arid regions of China. A strong positive relationship between sediment DOM stabilities, especially humification, and ecosystem degradation was found, consistent for the three lakes. Ultra-high-resolution mass spectrometry and structural equation modeling revealed that the changes of ecosystems affected sediment DOM stability through direct pathways (0.24), such as the contents of terrestrial DOM in lake DOM pool, and indirect pathways, including algae-mediated (0.43) and salinity-mediated pathways (0.22), which all increased the contents of refractory DOM in the lake DOM pool and sediments. Based on the fact that DOM stability changes could act on the ecosystem in turn, a possible positive feedback mechanism between ecosystem degradation and increased DOM stability was further inferred. These results suggested that the continuous increased stability of sediment DOM in may implies ecosystem degradation of lakes in the cold and arid regions. This study provides a new perspective for recognizing ecosystem evolution through sediment DOM and improves the understanding of the interaction of lake ecosystem evolution and the biogeochemical cycle of DOM.
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Lake ecological processes and nutrient patterns are increasingly affected by water level variation around the world. Still, the long-term effects of water level change on lake ecosystems and their implications for suitable lake level management have rarely been studied. Here, we studied the ecosystem dynamics of a mesotrophic lake located in the cold and arid region of northern China based on long-term paleo-diatom and fishery records. Utilizing a novel Copula-Bayesian Network model, possible hydrological-driven ecosystem evolution was discussed. Results show that increased nutrient concentration caused by the first water level drop in the early 1980s incurred a transition of sedimental diatoms towards pollution-resistant species, and the following water level rise in the mid-1980s brought about considerable external loading, which attributed to eutrophication and caused the miniaturization of fishery structure. In the 21st century, a continuous water level plummet further reduced the sediment diatom biomass and the fish biomass by altering nutrient concentration. However, with the implementation of the water diversion project in 2011, oligotrophic species increased, and the ecosystem developed for the better. From the perspective of water quality protection requirements and the ecological well-being of Lake Hulun, the appropriate water level should be around 542.42-544.15 m. In summary, our study highlights the coupling effect of water level and water quality on Lake Hulun ecosystem and gives shed to lake water level operation and management under future climate change and human activities.
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Teorema de Bayes , Diatomeas , Ecosistema , Monitoreo del Ambiente , Peces , Lagos , Lagos/química , Animales , China , EutrofizaciónRESUMEN
Herein, we have used the "on-the-fly" ring-polymer surface-hopping simulation method with the centroid approximation (RPSH-CA), in combination with the multireference OM2/MRCI electronic structure calculations to study the photoinduced dynamics of a green fluorescent protein (GFP) chromophore analogue in the gas phase, i.e., o-HBI, at 50, 100, and 300 K with 1, 5, 10, and 15 beads (3600 1 ps trajectories). The electronic structure calculations identified five new minimum-energy conical intersection (MECI) structures, which, together with the previous one, play crucial roles in the excited-state decay dynamics of o-HBI. It is also found that the excited-state intramolecular proton transfer (ESIPT) occurs in an ultrafast manner and is completed within 20 fs in all the simulation conditions because there is no barrier associated with this ESIPT process in the S1 state. However, the other excited-state dynamical results are strongly related to the number of beads. At 50 and 100 K, the nuclear quantum effects (NQEs) are very important; therefore, the excited-state dynamical results change significantly with the bead number. For example, the S1 decay time deduced from time-dependent state populations becomes longer as the bead number increases. Nevertheless, an essentially convergent trend is observed when the bead number is close to 10. In contrast, at 300 K, the NQEs become weaker and the above dynamical results converge very quickly even with 1 bead. Most importantly, the NQEs seriously affect the excited-state decay mechanism of o-HBI. At 50 and 100 K, most trajectories decay to the S0 state via perpendicular keto MECIs, whereas, at 300 K, only twisted keto MECIs are responsible for the excited-state decay. The present work not only comprehensively explores the temperature-dependent photoinduced dynamics of o-HBI, but also demonstrates the importance and necessity of NQEs in nonadiabatic dynamics simulations, especially at relatively low temperatures.
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Dissolved organic matter (DOM) constitutes the most active fraction in global carbon pools, with estuarine sediments serving as significant repositories, where DOM is susceptible to dynamic transformations. Anthropogenic nitrogen (N) and sulfur (S) inputs further complicate DOM by creating N-bearing DOM (DON) and S-bearing DOM (DOS). This study delves into the spatial gradients and transformation mechanisms of DOM, DON, and DOS in Pearl River Estuary (PRE) sediments, China, using combined techniques of UV-visible spectroscopy, Excitation-emission matrix (EEM) fluorescence spectroscopy, Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS), and microbial high-throughput sequencing. Results uncovered a distinct spatial gradient in DOM concentration, aromaticity (SUVA254), hydrophobicity (SUVA260), the content of substituent groups including carboxyl, carbonyl, hydroxyl and ester groups (A253/A203) of chromophoric DOM (CDOM), and the abundances of tyrosine/tryptophan-like protein and humic-like substances in fluorophoric DOM (FDOM). These all decreased from upper to lower PRE, accompanied by a decrease in O3S and O5S components, indicating seaward reduction in the contribution of terrestrial OM, especially anthropogenic inputs. Additionally, sediments exhibited a reduction in molecular diversity (number of formulas) of DOM, DON, and DOS from upper to lower PRE, with molecules tending towards a lower nominal oxidation state of carbon (NOSC) and higher bio-reactivity (MLBL), molecular weight (m/z) and saturation (H/C). While molecular composition of DOM remained similar in PRE sediments, the relative abundance of lignin-like substances decreased, with a concurrent increase in protein-like and lipid-like substances in DON and DOS from upper to lower PRE. Mechanistic analysis identified the joint influence of terrestrial OM, anthropogenic N/S inputs, and microbial processes in shaping the spatial gradients of DOM, DON, and DOS in PRE estuarine sediments. This study contributes valuable insights into the intricate spatial gradients and transformations of DOM, DON, and DOS within human-impacted estuarine sediments.
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Materia Orgánica Disuelta , Ríos , Humanos , Ríos/química , Espectrometría de Masas , Estuarios , CarbonoRESUMEN
The concentrations, spatial-temporal distribution, and influencing factors of 16 polycyclic aromatic hydrocarbons (PAHs) in the sediments of Poyang Lake were studied, and a quantitative source analysis of PAHs in different areas of the lake was conducted. PAHs were widespread within the sediments. The concentrations of ∑16PAHs in the surface sediments of all sites ranged from 203 to 2 318 µg·kg-1. The concentrations of PAHs in the surface sediments of the lake body were higher than those in the surface sediments of the inlet rivers. The ratio of PAHs in Poyang Lake was 4 rings > 5 rings > 6 rings > 3 rings > 2 rings; the composition of 4-ring PAHs was dominant, and its content accounted for 86.11% of ∑16PAHs. The 2- and 3-ring and some 4-ring PAHs, including Flua and Pyr, were more susceptible to SOM, and the 4 through 6-ring PAHs were more susceptible to ORP and heavy metals and other environmental factors. Spatially, the higher concentration of ∑16PAHs occurred in the area of the lake adjacent to Duchang County and Poyang County, where the terrain was relatively closed, and the water exchange with the surrounding area was less than that in other sections, which was not conducive to the migration, transformation, and degradation of pollutants. In the temporal distribution, the changes in PAHs concentration level and the development of GDP in Jiangxi Province showed high consistency, and the influence of economic development and human activities might have been the main reason for the increasing PAHs concentration level. The main sources of PAHs in surface sediments of Poyang Lake included petroleum pollution and oil and coal and biomass combustion sources, and there were some spatial differences in PAHs sources in different regions. This study can provide a reference for PAHs pollution in surface sediments of Poyang Lake, which is important for the ecological environmental protection and management of Poyang Lake.
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PURPOSE: The present study aimed to assess the clinical efficacy and imaging results of reconstruction of the medial patellofemoral ligament through a double bundle of single patellar tract and quadriceps tendons combined with medial displacement of lateral hemi-tibial tuberosity for treating low-grade recurrent patella dislocation. METHODS: Twenty-three patients with recurrent patellar dislocation, including ten males and 13 females, with 23 knee joints were enrolled according to the relevant criteria. Reconstruction of the medial patellofemoral ligament was performed through a double bundle of a single patellar tract and quadriceps tendons combined with medial displacement of lateral hemi-tibial tuberosity. Knee function was evaluated using visual analog scale (VAS) score, International Knee Documentation Committee (IKDC) score, Lysholm score, Tegner score, and Kujala score at pre- and postoperative stages. Patellar stability was assessed by CT scans measuring tibial tuberosity-trochlear groove (TT-TG) distance, lateral patella displacement (LPD), congruence angle (CA), and patellar tilt angle (PTA). RESULTS: All 23 patients were effectively followed up for 13-28 months (mean: 21.91 ± 4.14 months). At the last follow-up, the postoperative VAS score, IKDC score, Lysholm score, Tegner score, and Kujala score of 23 patients were 1.13 ± 0.82, 87.35 ± 3.17, 90.22 ± 1.28, 4.35 ± 0.65, and 89.26 ± 1.96, respectively, as compared to the preoperative values of 5.91 ± 1.13, 30.96 ± 5.09, 30.30 ± 2.98, 1.26 ± 0.62, and 27.87 ± 3.46, respectively, and these differences were statistically significant (P < 0.001). At the last follow-up, the postoperative TT-TG, LPD, CA, and PTA values of the 23 patients were 8.80 ± 1.85 mm, 6.01 ± 1.77 mm, 11.32 ± 6.18°, and 9.35 ± 2.88°, respectively, compared to the preoperative values of 18.77 ± 1.74 mm, 14.90 ± 4.07 mm, 37.82 ± 5.71°, and 23.58 ± 3.24°, respectively, and the differences were statistically significant (P < 0.001). No relevant complications were observed in the 23 patients. CONCLUSIONS: Reconstruction of the medial patellofemoral ligament through a double bundle of a single patellar tract and quadriceps tendons combined with medial displacement of lateral hemi-tibial tuberosity for treating low-grade recurrent patella dislocation showed satisfactory medium-term efficacy, and further investigations are required to confirm the long-term efficacy of this approach.
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Luxaciones Articulares , Inestabilidad de la Articulación , Luxación de la Rótula , Articulación Patelofemoral , Masculino , Femenino , Humanos , Rótula/cirugía , Luxación de la Rótula/diagnóstico por imagen , Luxación de la Rótula/cirugía , Articulación Patelofemoral/cirugía , Articulación de la Rodilla/diagnóstico por imagen , Articulación de la Rodilla/cirugía , Ligamentos Articulares/diagnóstico por imagen , Ligamentos Articulares/cirugía , Tendones , Inestabilidad de la Articulación/diagnóstico por imagen , Inestabilidad de la Articulación/cirugíaRESUMEN
Identification of key As mobilization processes in estuarine sediments is challenging due to the transitional hydrodynamic condition and the technical restriction of obtaining fine-scale results. Herein, high-resolution (µm to mm) and in situ profiling of As with associated elements (Fe, Mn, and S) by the diffusive gradients in thin-film (DGT) technique were applied and coupled with pore water and solid phase analysis as well as microbial high-throughput sequencing, to ascertain the driving mechanisms of As mobilization in the sediments of Pearl River Estuary (PRE). Significant diffusion fluxes of As from sediment to water were observed, particularly in the upper estuary. With the seaward increase of salinity, the driving mechanism of As mobilization gradually shifted from microbial-induced dissimilatory Fe reduction to saltwater-induced ion exchange. Correspondingly, the dominant Fe-reducing bacteria (FeRB) in sediments changed from the genera Clostridium_sensu_stricto_1 and Bacillus to Ferrimonas and Deferribacter. The presence of dissolved sulfide in deeper sediments contributes to As removal through the formation of As-S precipitates as supported by theoretical calculations. Fine-scale findings revealed seaward changes of As mobilization mechanism in the sediments of a human-impacted estuary and may benefit the understanding of As biogeochemical behavior in estuaries worldwide.
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OBJECTIVE: To explore clinical effect of attaching locking plate with bone grafting based on retaining the original intramedullary nail in treating non-union after intramedullary nail fixation of long shaft fractures of lower limbs. METHODS: A retrospective study was conducted on 20 patients treated with non-union fractures after intramedullary nailing of long shaft fractures of lower limbs from June 2015 to June 2020. All patients were treated with the original intramedullary nailing and bone grafting from the iliac bone, and were underwent open reduction plate internal fixation and bone grafting for old fractures. Among them, 14 were males and 6 were females, aged from 35 to 56 years old with an average of (42.2±9.6) years old. Nine patients were femoral shaft fracture and 11 patients were tibial shaft fracture. According to characteristics of fracture end nonunion, 6 patients were stable/atrophic, 9 patients were unstable/large, and 5 patients were unstable/atrophic. The nonunion time ranged from 8 to 12 months with an average of(9.8±2.0) months after the initial surgery. Visual analogue scale (VAS), knee range of motion, bone healing time, complications and fracture-end healing were recorded before and at the latest follow-up. RESULTS: All patients were followed up for 18 to 48 months with an average of (36.3±10.5) months. The incision of all patients were healed at stageâ without complications such as infection or internal fixation ruptur. Healing time of femur and tibia was (8.5±2.6) months and (9.5±2.2) months. Knee joint motion increased from preoperative (101.05±8.98) ° to postoperative (139.35±8.78) ° at the latest follow-up (t=-12.845, P<0.001). VAS decreased from preoperative (5.15±1.72) to postoperative (0.75±0.96) at the latest follow-up (t=11.186, P<0.001). CONCLUSION: On the basis of retaining the original intramedullary nail, the addition of locking plate internal fixation and autogenous iliac bone grafting have advantages of simple operation, less trauma, fewer complications and high fracture healing rate. It is one of the effective surgical schemes for the treatment of nonunion after intramedullary nail fixation of long bone fracture of lower extremity.
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Fracturas del Fémur , Fijación Intramedular de Fracturas , Fracturas no Consolidadas , Fracturas de la Tibia , Masculino , Femenino , Humanos , Adulto , Persona de Mediana Edad , Trasplante Óseo , Estudios Retrospectivos , Placas Óseas , Fijación Intramedular de Fracturas/efectos adversos , Fracturas del Fémur/cirugía , Fracturas del Fémur/complicaciones , Extremidad Inferior , Fracturas no Consolidadas/cirugía , Curación de Fractura , Fracturas de la Tibia/complicaciones , Clavos Ortopédicos , Resultado del TratamientoRESUMEN
Estuaries are significant sources of the ozone-depleting greenhouse gas N2O. However, owing to large spatial heterogeneity and discrete measurements, N2O emissions from estuaries are considerably uncertain. Microbial processes are disputed in terms of the dominant N2O production under severe human disturbance. Herein, combining real-time and high-resolution measurements with bioinformatics analysis, we accurately mapped the consecutive two-dimensional N2O distribution in the Pearl River Estuary (PRE), China, and revealed its underlying microbial mechanisms. Both the horizontal and vertical distributions of N2O concentrations varied greatly at fine scales. Supersaturated N2O concentrations (9.1 to 132.2 nmol/L) in the surface water decreased along the estuarine salinity gradient, with several emission hotspots scattering upstream. The vertical N2O distribution showed marked differences from complete mixing upstream to incomplete mixing downstream, with constant or changeable concentrations with increasing depth. Furthermore, spatially varied denitrifying and nitrifying microorganisms controlled the N2O production and distribution in the PRE, with denitrification playing the dominant role. The nirK-type and nirS-type denitrifying bacteria were the primary producers of N2O in the water and sediment columns, respectively. In addition, substrate concentration (NO3- and DOC) regulated N2O production by affecting key microbial processes, while physical influences (water-mass mixing and salt wedges) reshaped N2O distribution. With these information, a conceptual model of estuarine N2O production and distribution was constructed to generalize the possible biochemical processes under environmental constraints, which could provide insights into the N2O biogeochemical cycle and emission mitigation from a mechanistic perspective.
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Desnitrificación , Estuarios , Humanos , Óxido Nitroso/análisis , Ríos/química , Agua/análisisRESUMEN
Along with rapid urbanization and intensive human activities, polycyclic aromatic hydrocarbon (PAH) pollution in the Pearl River Estuary (PRE) and its effects on the microbial community have attracted extensive attention. However, the potential and mechanism of microbial degradation of PAHs across water and sediment habitats remain obscure. Herein, the estuarine microbial community structure, function, assembly process and co-occurrence patterns impacted by PAHs were comprehensively analyzed using environmental DNA-based approaches. The contamination and distribution of PAHs were jointly affected by anthropogenic and natural factors. Some of the keystone taxa were identified as PAH-degrading bacteria (i.e., genera Defluviimonas, Mycobacterium, families 67-14, Rhodobacteraceae, Microbacteriaceae and order Gaiellales in water) or biomarkers (i.e., Gaiellales in sediment) that were significantly correlated with PAH levels. The proportion of deterministic process in the high PAH-polluted water (76%) was much higher than that in the low pollution area (7%), confirming the significant effect of PAHs on the microbial community assembly. In sediment, the communities with high phylogenetic diversity demonstrated a great extent of niche differentiation, exhibited a stronger response to environmental variables and were strongly influenced by deterministic processes (40%). Overall, deterministic and stochastic processes are closely related to the distribution and mass transfer of pollutants, and substantially affect the biological aggregation and interspecies interaction within communities in the habitats.
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Microbiota , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Humanos , Hidrocarburos Policíclicos Aromáticos/análisis , Ríos/química , Estuarios , Agua , Filogenia , Contaminantes Químicos del Agua/análisis , Sedimentos Geológicos/química , Bacterias/genética , Bacterias/metabolismo , Contaminación del Agua , ChinaRESUMEN
In this work, we implemented an approximate algorithm for calculating nonadiabatic coupling matrix elements (NACMEs) of a polyatomic system with ab initio methods and machine learning (ML) models. Utilizing this algorithm, one can calculate NACMEs using only the information of potential energy surfaces (PESs), i.e., energies, and gradients as well as Hessian matrix elements. We used a realistic system, namely CH2NH, to compare NACMEs calculated by this approximate PES-based algorithm and the accurate wavefunction-based algorithm. Our results show that this approximate PES-based algorithm can give very accurate results comparable to the wavefunction-based algorithm except at energetically degenerate points, i.e., conical intersections. We also tested a machine learning (ML)-trained model with this approximate PES-based algorithm, which also supplied similarly accurate NACMEs but more efficiently. The advantage of this PES-based algorithm is its significant potential to combine with electronic structure methods that do not implement wavefunction-based algorithms, low-scaling energy-based fragment methods, etc., and in particular efficient ML models, to compute NACMEs. The present work could encourage further research on nonadiabatic processes of large systems simulated by ab initio nonadiabatic dynamics simulation methods in which NACMEs are always required.
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Lakes in cold and arid regions have weak hydrological connectivity to their watersheds and serious soil erosion by wind, and are particularly sensitive to changes in underlying surface process and global climate changes, which may result in unique carbon cycles across the terrestrial-aquatic interface and ecological impacts. However, the roles of input pathways of terrestrial dissolved organic matter (TDOM) to lakes in cold and arid regions, especially the effects of possible TDOM input caused by wind erosion, has not been fully understood. Taking a typical lake in cold and arid regions as an example, this study comprehensively investigated the characteristics and contributions of dissolved organic matter (DOM) input from different TDOM input pathways, ultimately highlighted the impacts of wind erosion from composition characteristics, historical changes and universal proofs. The results showed that DOM introduced by wind erosion contributed 37.34 % to all TDOM input, and exhibited the strongest humification and aromaticity, and greatest molecular weight and stability. The considerable input and refractory characteristics led to the differences in the TDOM distribution and DOM composition between the near-wind and far-wind shores of the lake. Moreover, historical analysis showed that wind erosion became the main way driving the changes in buried terrestrial organic matter in the lake through a combined action of precipitation and land cover changes after 2008. The pervasive importance of wind erosion pathways on TDOM inputs in the cold and arid regions was further demonstrated through the proof from two other representative lakes. The findings also shed light on the possible impacts of wind erosion on material distribution, aquatic productivity, and energy input in lake ecosystems. The study provides new insights to broaden the content of global lake-landscape interactions and regional ecosystem conservation.
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Ecosistema , Lagos , Materia Orgánica Disuelta , VientoRESUMEN
Ecological water replenishment is considered to be an important project to adjust river ecosystems with water shortages and degradation, and its impact on the water quality of the target river section deserves attention. By investigating the characteristics of inorganic ions and organic components of the Beijing section of the Yongding River (YDR) from upstream to downstream, the sources of inorganic ions and dissolved organic matter (DOM) during an ecological water replenishment event were analysed and discussed. This study illustrated the hydrochemical response to different supplemental water sources in three sections of the YDR (mountain gorge section (MGS), urban plain section (UPS), and suburb plain section (SPS)). The results showed that the total dissolved solids (TDS) and ion concentrations were significantly different (p < 0.001) in the three river sections due to different supplemental water sources. The highest concentration of TDS was found in the UPS (870.92 mg/L) replenished by reclaimed water, while the lowest concentration of TDS was found in the SPS (306.95 mg/L) replenished by the water of the South-to-North Water Diversion Project (SNWD). Despite the differences in the water sources of replenishment, the optical parameters of DOM and fluorescent components showed similar protein-like dominated endogenous source characteristics in the three river sections of the YDR, which was due to the influence of reservoir water (MGS and SPS) and reclaimed water (UPS). Our study emphasizes the importance of understanding the impact of different water sources on the water replenishment process, which provides a scientific reference for the management of ecological water replenishment.
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Ecosistema , Calidad del Agua , China , Beijing , Materia Orgánica Disuelta , IonesRESUMEN
The successful use of exosomes in therapy after myocardial infarction depends on an improved understanding of their role in cardiac signaling and regulation. Here, we report that exosomes circulating after myocardial infarction (MI) carry LncRNA TUG1 which downregulates angiogenesis by disablement of the HIF-1α/VEGF-α axis and that this effect can be counterbalanced by remote ischemic conditioning (RIC). Rats with MI induced through left coronary artery ligation without (MI model) and with reperfusion (ischemia/reperfusion I/R model) were randomized to RIC, or MI (I/R) or sham-operated (SO) control. Data from one cohort study and one randomized-controlled trial of humans with MI were also utilized, the former involving patients who had not received percutaneous coronary intervention (PCI) and the latter patients with PCI. Exosome concentrations did not differ between intervention groups (RIC vs. control) in rats (MI and I/R model) as well as humans (with and without PCI). However, MI and I/R exosomes attenuated HIF-1α, VEGF-α, and endothelial function. LncRNA TUG1 was increased in MI and I/R exosomes, but decreased in SO and RIC exosomes. HIF-1α expression was downregulated with MI and I/R exosomes but increased with RIC exosomes. Exosome inhibition suppressed HIF-1α upregulation through RIC exosomes. VEGF-α was identified as HIF-1α-regulated target gene. Knockdown of HIF-1α decreased VEGF-α, endothelial cell capability, and tube formation. Overexpression of HIF-1α exerted opposite effects. Transfection and co-transfection of 293 T cells with exosome-inhibitor GW4869 and HIF-1α inhibitor si-HIF-1α confirmed the exosomal-LncRNA TUG1/HIF-1α/VEGF-α pathway. LncRNA TUG1 is a potential therapeutic target after MI with or without reperfusion through PCI.
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Infarto del Miocardio , Intervención Coronaria Percutánea , ARN Largo no Codificante , Humanos , Ratas , Animales , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Estudios de Cohortes , Factor A de Crecimiento Endotelial Vascular/metabolismo , Subunidad alfa del Factor 1 Inducible por Hipoxia/genéticaRESUMEN
Estimation of large-scale and high-precision water quality parameters is critical in explaining the spatiotemporal dynamics and the driving factors of water quality variability, especially in areas with environmental complexity (e.g., crisscrossing waterways, high flood risk in rainy season and seawater invasion). Thus, in this study, a retrieval algorithm was developed to predict chlorophyll-a (Chl-a), total nitrogen (TN) and total phosphorus (TP) concentrations in the Pearl River Estuary (PRE) based on a large amount of in situ measurements and Landsat 8 remote sensing images. Random Forest (RF) machine learning was conducted to identify the relationship between environmental indicators (pH, turbidity, conductivity, total dissolved solids and water temperature), Chl-a, TN and TP. The results showed that the NIR/R Binomial algorithm for Chl-a estimation presented appreciable reliability with R2 of 0.7429, root mean square error (RMSE) of 1.2089 and mean absolute percent error (MAPE) of 15.33%. The water quality variation in the PRE showed a characteristic of overall improvement and regional deterioration with average concentrations of 7.28 µg/L, 1.15 mg/L and 0.12 mg/L for Chl-a, TN, and TP respectively. Turbidity and pH were identified as the most important indicators to explain Chl-a (52.86%, 39.91%), TN (52.38%, 40.57%) and TP (55.23%, 40.03%) variation. Agricultural pollution was the main pollution source due to the intensive application of fertilizer and increased field size. Besides, land use patterns (e.g., increasing farmland but decreasing forest) greatly influenced water quality from 2010 to 2020. Moreover, light limitation caused by high turbidity reduced the algae productivity and further lowered the Chl-a concentration. The driving factors for regional water quality variations were anthropologically dominated and supplemented by climate change. This study improved the monitoring accuracy of regional water environment and provided quantitative early warning of water pollution events for environmental practitioners, so as to achieve long-term monitoring, precise pollution management and efficient water resources management.
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Fósforo , Ríos , Algoritmos , China , Clorofila/análisis , Clorofila A , Monitoreo del Ambiente , Estuarios , Eutrofización , Fertilizantes , Lagos , Nitrógeno/análisis , Fósforo/análisis , Reproducibilidad de los ResultadosRESUMEN
Brownification is an increasingly concerning phenomenon faced by aquatic ecosystems in the changing environments, and the microbiome plays an irreplaceable role in material circulation and food web construction. Insight into the influence of brownification on microbial communities is crucial from an ecological standpoint. In this study, we simulated brownification using a the mesocosm system and explored the relationship between the characteristics of microbial communities and brownification using excitation-emission matrix (EEM) fluorescence spectroscopy and ultraviolet (UV) spectroscopy combined with high-throughput amplicon sequencing techniques. The results showed that brownification reduced the richness of the microbial community and selectively promoted the growth of nitrogen-cycling microorganisms, including hgcI_clade, Microbacteriaceae, and Limnohabitans. Brownification affected microbial communities by altering the carbon source composition and underwater spectrum intensity; UV, blue, violet, and cyan light were significantly (p < 0.05) correlated with microbial community richness, and random forest analysis revealed that UV, C1 (microbial humic-like), and C3 (terrestrial humic-like) were the major factors significantly influencing microbiome variation. We found that brownification affected microorganisms in shallow lakes, especially nitrogen cycling microorganisms, and propose that controlling terrestrial material export is an effective strategy for managing freshwater brownification.
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Ecosistema , Rayos Ultravioleta , Lagos/química , Carbono , NitrógenoRESUMEN
Organic phosphorus (OP) is one of the main forms of phosphorus in lake ecosystems. Mounting evidence has shown that sediment OP has become a major but underestimated issue in addressing lake eutrophication and algal bloom. However, a holistic view of sediment OP remains missing. This review aims to provide an overview of progress on the studies of OP in lake sediments, focusing on the contribution of OP to internal P loading, its potential role in algal bloom, and the migration and transformation. In addition, this work systematically summarized current methods for characterizing OP content, chemical fraction, composition, bioavailability, and assessment of OP release in sediment, with the pros and cons of each method being discussed. In the end, this work pointed out following efforts needed to deepen the understanding of sediment OP, namely: (1) In-depth literature review from a global perspective regarding the contribution of sediment OP to internal P loading with further summary about its pattern of distribution, accumulation and historical changes; (2) better mathematical models for describing drivers and the linkages between the biological pump of algal bloom and the replenishment of sediment OP; (3) fully accounting the composition and molecular size of OP for better understanding its transformation process and mechanism; ; (4) developing direct, high-sensitivity and combined techniques to improve the precision for identifying OP in sediments; (5) establishing the response of OP molecular properties and chemical reactivity to OP biodegradability and designing a comprehensive and accurate composite index to deepen the understanding for the bioavailability of OP; and (6) integrating fundamental processes of OP in current models to better describe the release and exchange of P in sediment-water interface (SWI). This work is expected to provide critical information about OP properties and deliver perspectives of novel characterization methods.
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Lagos , Contaminantes Químicos del Agua , China , Ecosistema , Monitoreo del Ambiente , Eutrofización , Sedimentos Geológicos/química , Lagos/química , Fósforo/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
To reveal the characteristics of organic phosphorus release from lake sediments and its potential impact on water quality, six lake sediments from Yunnan Plateau and the middle and lower reaches of the Yangtze River in China were selected. We studied the differences in the kinetics of dissolved organic phosphorus (DOP) and dissolved inorganic phosphorus (SRP) release from sediments. The effects of organic phosphorus morphology and dissolved organic matter (DOM) characteristics on sediment phosphorus release were investigated, and the water quality risks of sediment DOP release were discussed. The results showed that:â the release kinetics of sediment DOP and SRP were similar; both followed the second-order kinetic model, starting with a rapid release phase, followed by a slow release, and the release curve gradually leveled off and reached the maximum release. â¡ The release of organic phosphorus was related to organophosphorus morphology and organic matter. Active organic phosphorus (LOP) and medium active organic phosphorus (MLOP) were the DOP forms mainly released into the overlying water during the rapid release phase. The proportion of LOP and MLOP to total organic phosphorus (DTP) decreased in the late release stage, whereas the proportion of non-active organic phosphorus (NLOP) increased; further, the degree of humification and aromaticity of organic matter gradually increased with phosphorus release, and its activity decreased, resulting in a slower release rate at the later stage. ⢠Compared with that of SRP, the risk of DOP release was higher, accounting for 47%-77% of the total amount of DTP. It was also found that the higher the nutrient level of the lake, the greater the release of DOP and the higher the water quality risk. Therefore, not only the release of inorganic phosphorus but also that of organic phosphorus should be of concern in the process of phosphorus release from lake sediments to prevent the underestimation of phosphorus release and water quality risk.
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Fósforo , Contaminantes Químicos del Agua , China , Sedimentos Geológicos , Cinética , Lagos , Fósforo/análisis , Contaminantes Químicos del Agua/análisis , Calidad del AguaRESUMEN
The new coronavirus outbreak has been officially declared a global pandemic by the World Health Organization. To grapple with the rapid spread of this ongoing pandemic, most countries have banned indoor and outdoor gatherings and ordered their residents to stay home. Given the developing situation with coronavirus, mental health is an important challenge in our society today. In this paper, we discuss the investigation of social media postings to detect signals relevant to depression. To this end, we utilize topic modeling features and a collection of psycholinguistic and mental-well-being attributes to develop statistical models to characterize and facilitate representation of the more subtle aspects of depression. Furthermore, we predict whether signals relevant to depression are likely to grow significantly as time moves forward. Our best classifier yields F-1 scores as high as 0.8 and surpasses the utilized baseline by a considerable margin, 0.173. In closing, we propose several future research avenues.
Asunto(s)
COVID-19 , COVID-19/epidemiología , Depresión/diagnóstico , Depresión/epidemiología , Humanos , Salud Mental , Pandemias , SARS-CoV-2RESUMEN
How to maintain harmful algal blooms under phosphate-limitation is still an open question in mesotrophic/eutrophic lakes. Little evidence for the importance of suspended particulate matter (SPM) in mediating phosphorus cycling and contributing to eutrophication has been generated for aquatic ecosystems, especially in coupled river-lake systems. In this study, we examined phosphorus transport and redistribution in a river-lake system in the Lake Erhai basin by establishing the relations between phosphorus distribution and phosphorus sorption behavior on SPM, and predicted how changes in the quality and quantity of SPM might influence phosphorus cycling by laboratory experiments and modeling. During the wet seasons, TP pool shifted from being dominated by total dissolved phosphorus (TDP) in the Miju River and estuary regions (73±5%) to being dominated by total particulate phosphorus (TPP) (74±11%) in Lake Erhai. The detritus-SPM in the Miju River as a P-sink and phytoplankton-SPM in Lake Erhai as a P-source buffered TDP levels during the wet seasons, which attributed to P activity and phytoplankton-POC of SPM. Increasing SPM concentrations could enhance the P-buffering. When C0 ≤ 5 µmol/L and phytoplankton-SPM ≥ 16 mg/L, P release increased by 50%-300%; when C0 ≥ 5 µmol/L and detritus-SPM ≥ 16 mg/L, P removal could exceed 30%. This study highlights two distinctive roles of SPM in regulating P cycling during transport from rivers to lakes. Especially the phytoplankton-SPM to buffer phosphate-limitation during algal blooms should not be ignored, which could provide theoretical references for the mechanism of continued algal blooms in mesotrophic lakes.