RESUMEN
Heatstroke not only directly induces cell injury, but also causes large amounts of inflammatory mediators release and cells with extensive biological activities to induce a systemic inflammatory response and immune dysfunction. This study aimed to observe the effects of JAK2 inhibitor AG490 on the brain injury and inflammatory responses of rats with systemic heatstroke. Under the light microscope, the hippocampus tissues of rat with heatstroke were edema and apoptotic rate was increased. Up-regulation of malondialdehyde (MDA), nitric oxide synthase (iNOS), reactive oxygen species (ROS) and down-regulation of superoxide dismutase (SOD) were also found after heatstroke in rats, which compared with that of the control group. Heatstroke induced inflammation factors secretions and up-regulated levels of matrix metallopeptidase 2 and 9 (MMP2 and MMP-9) and systemic inflammatory response molecules including intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor-beta 1 (TNF-ß1) and cyclooxygenase-2 (COX-2). However, the JAK2 inhibitor AG490 was significantly attenuated the brain injury and inflammatory responses induced by heatstroke in rats. The survival time of heatstroke rats showed that AG490 notably lived longer than heatstroke rats without AG490 treatment. These findings suggest that AG490 may prevent the occurrence of heatstroke via inhibiting the JAK2/STAT3 pathway and the systemic inflammatory responses.
Asunto(s)
Antiinflamatorios/farmacología , Golpe de Calor/tratamiento farmacológico , Hipocampo/efectos de los fármacos , Inflamación/prevención & control , Janus Quinasa 2/antagonistas & inhibidores , Fármacos Neuroprotectores/farmacología , Inhibidores de Proteínas Quinasas/farmacología , Factor de Transcripción STAT3/metabolismo , Tirfostinos/farmacología , Animales , Apoptosis/efectos de los fármacos , Edema Encefálico/enzimología , Edema Encefálico/inmunología , Edema Encefálico/patología , Edema Encefálico/prevención & control , Ciclooxigenasa 2/metabolismo , Golpe de Calor/enzimología , Golpe de Calor/inmunología , Golpe de Calor/patología , Hipocampo/enzimología , Hipocampo/inmunología , Hipocampo/patología , Inflamación/enzimología , Inflamación/inmunología , Inflamación/patología , Molécula 1 de Adhesión Intercelular/metabolismo , Janus Quinasa 2/metabolismo , Masculino , Malondialdehído/metabolismo , Metaloproteinasa 2 de la Matriz/metabolismo , Metaloproteinasa 9 de la Matriz/metabolismo , Óxido Nítrico Sintasa de Tipo II/metabolismo , Estrés Oxidativo/efectos de los fármacos , Ratas Sprague-Dawley , Especies Reactivas de Oxígeno/metabolismo , Transducción de Señal/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Factores de Tiempo , Factor de Crecimiento Transformador beta1/metabolismoRESUMEN
The detection of the infertile eggs and fertile eggs by the near infrared diffuse reflectance spectra was proposed. Models based on different band regions range, different principal component numbers and the different spectral pre-processing methods were compared and the optimal calibration model was established. The results show that qualitative forecasting model of hatching eggs is established by Mahalanobis Distance, which is with band regions range being 4 119.20-9 881.46 cm(-1), principal component number being 19 and spectral pre-processing method being SNV + first derivative + Norris differential filter. The precision rate of calibration set is 92.5% and that of validation set is 91.67%. The study provides a new way for nondestructive testing of the fertile eggs and infertile eggs prior to incubation.