Strong gelation capacity of a pectin-like polysaccharide in the presence of K+ ion.

In the present study, a pectin-like apple polysaccharide (AP) obtained by metal precipitation technique was demonstrated to show strong gelling capacity in the presence of K+ ion upon cooling. Increasing amount of K+ addition monotonically promoted the gelation of AP, as characterized by the increased gelation temperature (Tgel), gel melting temperature (Tmelt) and the gel strength. Compared with K+ ion, Na+ was unable to induce AP gelation even at high ionic concentrations, but other monovalent cations (Rb+, Cs+) can induce the gelation as in the case of K+ addition. At room temperature, the minimum cationic concentration as required to induce AP gelation followed the order of K+ ≈ Cr+ (8 mM) > Rb+ (3.5 mM), indicating that cationic radius (Na+ < K+ < Rb+ < Cs+) played a dominant role in inducing AP gelation, but other factors may also be involved. Finally, the gelation behavior of AP in the presence of K+ was explained as the suppressed intermolecular electrostatic repulsion between AP chains due to the strong electrostatic shielding effect of K+, which led to the formation of a gel network mediated by intermolecular hydrogen bonding. This reported gelation property may allow AP to find application as a new gelling polysaccharide.

Gelation behaviors and mechanism of a new pectic polysaccharide from apple pomace as a potential gelatin substitute.

In this study, we reported a pectic polysaccharide industrially obtained from apple pomace by metal ion precipitation technique showing an unexpected gelation behavior. Structurally, this apple pectin (AP) is a macromolecular polymer with a weight-average molecular weight (Mw) of 361.7 kDa, and DM (degree of methoxylation) of 12.5 %, comprising 60.38 % glucose, 19.41 % mannose, 17.60 % galactose and 1.00 % rhamnose and 1.61 % glucuronic acid. The low acidic sugar percentage relative to the total monosaccharide amount indicated a high branching structure of AP. On addition of Ca2+ ions, AP exhibited a remarkable gelling ability upon cooling its heat solution to low temperature (e.g., 4 °C). However, at room temperature (e.g., 25 °C) or in the absence of Ca2+, no gel was formed. At a fixed pectin concentration (0.5 %, w/v), AP showed increasing gel hardness and gelation temperature (Tgel) with CaCl2 concentration increasing to 0.05 % (w/v); however, further addition of CaCl2 weakened AP gels and even abolished the gelation. On reheating, all gels melted below 35 °C, which suggests the potential use of AP as a gelatin substitute. The gelation mechanism was explained as an intricate balance of the synchronous formation of hydrogen bond and Ca2+ crosslinks between AP molecules during cooling.