Novel potential tumor biomarkers: Circular RNAs and exosomal circular RNAs in gastrointestinal malignancies.

BACKGROUND: Circular RNAs (circRNAs) are structural ubiquitous RNA molecules. Accumulating evidences have elucidated that circRNAs play essential roles in the pathogenesis of diseases including cancers. Exosomal circRNAs are those circRNAs stably existing in exosomes and having high clinical values as novel potential diagnostic biomarkers of many diseases. Gastrointestinal (GI) malignancies, including pancreatic cancer, colorectal cancer, hepatocellular carcinoma (HCC), and gastric cancer, are leading causes of mortality worldwide and a major global health burden. However, no ideal tumor biomarkers of screening early GI cancers are currently available. METHODS: We collected data through Web of Science. The search terms used were as follows: circular RNA, circRNA, exosomes, exosomal circRNAs, biomarkers, gastrointestinal malignancies, pancreatic cancer, hepatocellular carcinoma, HCC, gastric cancer, colorectal cancer, physiological functions, biogenesis, molecular mechanism. Only articles published in English were included. RESULTS: We found that several circRNAs and exosomal circRNAs have been used as potential biomarkers to screen GI cancers including pancreatic cancer (hsa_circ_0001649, circ_0007534, circ_0030235, circRHOT1, circZMYM2, circ-LDLRAD3, chr14:101402109-101464448C, chr4:52729603-52780244C, circ-IARS, and circ-PDE8A), HCC (circSETD3, circADAMTS13, hsa_circ_0007874, hsa_circ_104135, circFBLIM1, cSMARCA5, circRNA-100338, and circPTGR1), colorectal cancer (hsa_circ_0001178, hsa_circ_0000826, hsa_circ_0004771, circDDX17, circITGA7, and circHIPK3), and gastric cancer (hsa_circ_0074362, circNRIP1, circAKT3, circ-DONSON, circPSMC3, circ-KIAA1244, circPVRL3, circPVT1, hsa_circ_0000096, ciRS-133, hsa_circ_0001017, and hsa_circ_0061276). CONCLUSION: CircRNAs and exosomal circRNAs have the potential high clinical diagnostic values for GI malignancies.

Identification of hsa_circ_0005654 as a new early biomarker of gastric cancer.

Gastric cancer is one of the most common cancers in the world. However, current medical technologies have not identified a reliable method to cure advanced gastric cancer, and early gastric cancer is difficult to diagnose. Therefore, we focused on circular RNAs (circRNAs) that have been proven to be involved in the carcinogenesis of gastric cancer. We first used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to evaluate the expression levels of hsa_circ_0005654 in 301 tissues, including 122 healthy gastric mucosa samples, 68 paired tissues from early gastric cancer and adjacent nontumor mucosae obtained by submucosal dissection, and 43 chronic gastritis tissues. Then, we analyzed the relationship between the expression levels of hsa_circ_0005654 and the clinicopathological characteristics of patients with early gastric cancer. We ultimately confirmed the clinical diagnostic value of hsa_circ_0005654 through generating receiver operating characteristic (ROC) curves and comparing the areas under the ROC curves (AUCs).Our data revealed that hsa_circ_0005654 was significantly downregulated in early gastric cancer tissues compared with matched normal mucosae (P< 0.001). Meanwhile, the expression levels of hsa_circ_0005654 in early gastric cancer tissues were also obviously lower than those in chronic gastritis tissues (P< 0.001). The AUCs of early gastric cancer tissues vs. paired normal adjacent mucosae, and that of early gastric cancer vs. healthy controls, were 0.927 and 0.924, respectively. These results clearly demonstrated that hsa_circ_0005654 may serve as a new and promising diagnostic biomarker for screening early gastric cancer. The AUC, sensitivity and specificity of hsa_circ_0005654 are significantly higher than those of present gastric cancer associated-biomarkers.

Hyaluronic acid (HA)-based hydrogels for full-thickness wound repairing and skin regeneration.

In this work, two kinds of hyaluronic acid (HA)-based hydrogels were fabricated: one is made from physical freezing-thawing of HA solution (HA1), and the other is from chemical cross-linking of HA and polysaccharide (HA2). They were applied to repair full-thickness skin defects with New Zealand rabbits as the test animals, using powder HA and cotton dress as the references. The wound starts to heal after wounds were disinfected with iodine followed by coating with HA2, HA1, HA and cotton dress (the control), respectively. They were recorded as 4 treatments (groups), HA2, HA1, HA and the control. The healing progress was followed and tested in the duration of 56 days, and the biological repairing mechanism was explored. From the wound area alteration, white blood cell (WBC) measurements and H&E staining, HA2 was the most promising treatment in promoting the wound healing with least serious scar formation. Immunochemistry analyses and real-time PCR tests of the bio-factors involved in the wound healing, vascular endothelial growth factor (VEGF), alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1 (TGF-ß1), exhibited that HA2 enhanced VEGF and α-SMA secretion but reduced TGF-ß1 expression at early stage, which alleviated the wound inflammation, improved the skin regeneration and relieved the scar formation.

[Relation between coronary artery disease and polymorphism of angiotensin converting enzyme gene].

This study was aimed to determine the relationship between coronary artery disease(CAD) and insertion/deletion(I/D) polymorphism of the angiotensin coverting enzyme(ACE) gene. The ACE genotypes of 105 patients with CAD (50 of them were accompanied by essential hypertension (EH)) and 102 healthy people were detected by polymerase chain reaction. The results showed that in terms of ACE genotypes, no significant difference was noted between CAD group and control group, nor was it observed between CAD group and CAD accompanied by EH group, and nor was it seen between CAD/CAD accompanied by EH group and control group. These findings suggest that there is no relationship between the polymorphism of ACE gene and CAD (including those accompanied by EH).