RIN1 promotes renal cell carcinoma malignancy by activating EGFR signaling through Rab25.

We previously identified the important role of RIN1 expression in the prognosis of clear cell renal cell carcinoma (ccRCC). The role of RIN1 in ccRCC malignancy and underlying molecular mechanisms remain unclear. Here we report that ccRCC cells and tissues expressed more RIN1 than normal controls. Gain-of-function and loss-of-function studies demonstrated that RIN1 enhanced ccRCC cell growth, migration and invasion abilities in vitro and promoted tumor growth and metastasis in vivo. Mechanistic studies revealed that RIN1 has an activating effect on EGFR signaling in ccRCC. In addition, we unveil Rab25, a critical GTPase in ccRCC malignancy, as a functional RIN1 interacting partner. Knockdown of Rab25 eliminated the augmentation of carcinoma cell proliferation, migration and invasion by ectopic RIN1. We also confirmed that RIN1 and Rab25 expression correlates with the overall-survival of ccRCC patients from TCGA. These findings suggest that RIN1 plays an important oncogenic role in ccRCC malignancy by activation of EGFR signaling through interacting with Rab25, and RIN1 could be employed as an effective therapeutic target for ccRCC.

Beneficial Effect of Educational and Nutritional Intervention on the Nutritional Status and Compliance of Gastric Cancer Patients Undergoing Chemotherapy: A Randomized Trial.

Surgery combined with chemotherapy is the standard treatment for gastric cancer (GC); however, chemotherapy-relative adverse effects are common and result in malnutrition and a poor prognosis. In addition, compliance to postoperative chemotherapy remains a problem. This study aimed to prospectively investigate the effect of educational and nutritional interventions on the nutritional status and compliance of GC patients undergoing postoperative chemotherapy. A total of 144 GC patients were randomized into an intervention group that received intensive individualized nutritional and educational interventions during the entire course of chemotherapy and control group that received basic nutrition care and health education during hospitalization. The nutritional status and compliance between the two groups were compared. The interventions significantly improved calorie and iron intake within 24 h after the first chemotherapy session, and improved patients' weight, hemoglobin, total serum protein, and albumin levels during the entire course of chemotherapy. The compliance rate with chemotherapy was significantly higher in the intervention group than in the control group (73.61% vs. 55.56%, P = 0.024). A combination of nutritional and educational interventions provided beneficial effect on the nutrition status and compliance of gastric patients undergoing postoperative chemotherapy, which is worthy of clinical application.

miR-106b-5p promotes renal cell carcinoma aggressiveness and stem-cell-like phenotype by activating Wnt/ß-catenin signalling.

PURPOSE: To examine the role of miR-106b-5p in regulating the cancer stem-cell-like phenotype in clear cell renal cell carcinomas (ccRCC). EXPERIMENTAL DESIGN: Real-time PCR was performed to evaluate miR-106b-5p levels in ccRCC cell lines and patients specimens. A series of in vivo and in vitro assays were performed to confirm the effect of miR-106b-5p on ccRCC stemness phenotype. RESULTS: ccRCC cells and tissues expressed more miR-106b-5p than normal controls. Gain- and loss-of-function studies demonstrated that overexpression of miR-106b-5p in ccRCC cells increased the spheres formation ability and the proportion of side population cells. Ectopic expression of miR-106b-5p in ccRCC cells increased tumour growth rates and the number of metastatic colonies in the lungs by using an orthotopic kidney cancer model and a tail vein injection model, respectively. Mechanistic studies revealed that, miR-106b-5p has an activating effect on Wnt/ß-catenin signalling. miR-106p-5p overexpression simultaneously targets multiple negative regulators of the Wnt/ß-catenin pathway, namely, LZTFL1, SFRP1 and DKK2. In addition, we also confirmed that miR-106b-5p and its targets expression correlates with the overall-survival of ccRCC patients from TCGA. CONCLUSIONS: These findings suggest that miR-106b-5p mediates the constitutive activation of Wnt/ß-catenin signalling, likely serving as a potential therapeutic target for ccRCC.

MicroRNA-320 inhibits cell proliferation in glioma by targeting E2F1.

MicroRNAs (miRs) are a class of small non-coding RNAs that are involved in the regulation of gene expression, and in cancer development and progression. In the present study, miR-320 expression was found to be significantly reduced in glioma tissue in comparison with that in adjacent healthy tissues. In the present study, in vitro analyses demonstrated that overexpression of miR-320 inhibited cell proliferation and metastasis, while antisense miR-320 oligonucleotides enhanced cell proliferation and migration in U251 and SHG-44 glioma cell lines, compared with that in negative control cells. Protein expression of E2F1, a cell-cycle regulator, was negatively regulated by miR-320. Therefore, the present study provides novel insights into the association between miR-320 and glioma development.

Relationships between PTEN gene mutations and prognosis in glioma: a meta-analysis.

We conducted a meta-analysis in order to investigate the relationships between PTEN gene mutations and the prognosis in glioma. The following electronic databases were searched for relevant articles without any language restrictions: Web of Science (1945 ~ 2013), the Cochrane Library Database (Issue 12, 2013), PubMed (1966 ~ 2013), EMBASE (1980 ~ 2013), CINAHL (1982 ~ 2013), and the Chinese Biomedical Database (CBM) (1982 ~ 2013). Meta-analyses were conducted using the STATA software (Version 12.0, Stata Corporation, College Station, Texas USA). Hazard ratio (HR) with its corresponding 95 % confidence interval (95%CI) was calculated. Six independent cohort studies with a total of 357 glioma patients met our inclusion criteria. Our meta-analysis results indicated that glioma patients with PTEN gene mutations exhibited a significantly shorter overall survival (OS) than those without PTEN gene mutations (HR = 3.66, 95%CI = 2.02 ~ 5.30, P < 0.001). Ethnicity-stratified subgroup analysis demonstrated that PTEN gene mutations were closely linked to poor prognosis in glioma among Americans (HR = 3.72, 95%CI = 1.72 ~ 5.73, P < 0.001), while similar correlations were not observed among populations in Sweden, Italy, and Malaysia (all P > 0.05). Our meta-analysis provides direct and strong evidences for the speculation of PTEN gene mutations' correlation with poor prognosis of glioma patients.

[Functional evaluation of a "two in one" urethroplasty for hypospadias].

OBJECTIVE: To assess the functional result of a "Two in One" urethroplasty which combined oral mucosa graft and local flap. METHODS: 17 patients with hypospadias underwent a "Two in One" urethroplasty, which combined buccal mucosa and local flap for urethral reconstruction. Uroflowmetry was performed 1 day before and 1 year after operation. The urine flow rate, voided volume and urine flow curves were detected using a rotating sensor. The results of maximum urine flow rate (Qmax) were expressed as percentiles and compared to the Toguri value from normal children. RESULTS: Before corrective operation, 12 of 17 patients (70.6%) produced a plateau urine flow curve. 5 patients (29.4%) produced a very low flow curve. The average maximum flow rate was (7.89 +/- 2.29) ml/s per second compared to Toguri values, 12 of 17 patients (70.6%) had a Qmax below the normal 5th percentile. After a "Two in One" urethroplasty, a hell-shaped curve was obtained in 10 patients (58.8%). The maximum flow rate was (11.30 +/- 3.01) mL/s per second. 7 of 17 patients (41.2%) had a Qmax above the normal 25th percentile, 8 patients (47.1%) had a Qmax between the normal 25th percentile and 5th percentile, only 2 patients (11.8%) had a Qmax below the 5th percentile. CONCLUSIONS: The functional result of the "Two in One" urethroplasty is ideal. The maximum urine flow rate of the patients increases after the operation.

[Muscles reposition in correction of nasal deformity after unilateral cleft lip].

OBJECTIVE: To investigate the effective method for correction of nasal deformity after unilateral cleft lip. METHODS: 50 cases with secondary nasal deformity after unilateral cleft lip were retrospectively analyzed. All the patients underwent nasal and lip muscle reposition operation to restore the symmetry of nasal alar. RESULTS: The nasal deformity was greatly improved in all the 50 cases. The malposition of nasal column and nasal alar was corrected. The symmetry was markedly improved. The measurement before and after operation showed significant difference ( P<0.05). CONCLUSIONS: The reasons of secondary nasal deformity after unilateral cleft lip are complicated. The muscle reposition operation can effectively improve the deformity.

[Protective effects of Chinese herbal medicine Zuogui Pill on retina ganglion cells after optical nerve clipping injury in rats].

OBJECTIVE: To investigate the protective effects of Zuogui Pill (ZGP), a compound Chinese herbal medicine, on retina ganglion cells (RGCs) in rats after optical nerve clipping injury. METHODS: Optic nerves in Sprague-Dawley rats were crushed intraorbitally at 2 mm behind eyeball by using a forceps with a 140-gram chucking power for 30 s to induce unilateral injured model. The injured rats were fed with ZGP (ZGP group, 4.0 g/kg body weight each day) and equal volume of normal saline (injury group) respectively. Morphological changes in the retina were observed and the RGCs were quantified in the specimens. Nestin and glial fibrillary acidic protein (GFAP) expressions in the retina were detected by immunohistochemical fluorescence staining at different time points after nerve injury. RESULTS: After optical never crushing, the thickness of retina and the number of RGCs were significantly decreased in injury group as compared with the normal group. Nearly 50% of the total RGCs were disappeared at the 4th week in the injury group. The expression levels of nestin and GFAP in the retina were markedly increased after optical never crushing at the 2nd week and this lasted to the 8th week, and gradually decreased to the normal level at the 16th week. In contrast, the arrangement of retinal cells was regular in the ZGP group, and the number of survival RGCs was greater than that of the injury group. Meanwhile, the nestin and GFAP expressions in retina were significantly enhanced as compared with the injury group at all time points. CONCLUSION: ZGP may have protective effects on rat optic nerve and RGCs after contusion by promoting the nestin and GFAP expressions in Müller cells of the retina.

Correction of secondary bilateral complete cleft lip and whistling deformities: the advance and rearrangement of the bilateral lip tissues.

BACKGROUND: This report introduces a new method for correcting secondary deformities of bilateral complete cleft lip (BCCL) and assesses its short-term results. METHODS: In this series, 30 patients (21 males and 9 females) ages 4-27 years (mean age 14 years, 2 months) who had previously undergone surgery to correct BCCL using the lateral columellar flap method or the straight-line method were treated using the authors' new curved-line method. Points were marked, and curved lines were used to link the points, with subsequent rearrangement of the lip tissues. Photographic documentation of lip heights was obtained before and after surgery, and short-term patient satisfaction was evaluated using a questionnaire. RESULTS: At 6 months, the mean increases in central upper vermilion height and the vermilion heights of Cupid's bow points were 152 ± 20% (central upper vermilion), 87 ± 34% (left Cupid's bow point), and 91 ± 5% (right Cupid's bow point) (P ≤ 0.05). Of the 30 patients, 21 were very satisfied with their results, whereas seven patients graded their results as good. CONCLUSIONS: The technique of advancing and rearranging the bilateral lip tissues is a simple and effective method for correcting secondary deformities of BCCL.

Split-face comparison of intense pulsed light and nonablative 1,064-nm Q-switched laser in skin rejuvenation.

BACKGROUND: Multiple nonablative skin rejuvenation techniques have been used to improve facial aging. OBJECTIVE: To compare rejuvenation efficiency of intense pulsed light (IPL) with nonablative 1,064-nm Q-switched laser in Asian patients. MATERIALS & METHODS: Twelve female subjects were enrolled and received five sessions of treatments at 2-week intervals. A split-face study was performed, with IPL applied to the left side of the face and nonablative 1,064-nm Q-switched laser to the right side. RESULTS: All assessments showed significant skin rejuvenation. For the improvement of skin texture, pore size, and sebum secretion, similar efficiency from laser and IPL was observed. For lightening of skin tone and macula, the IPL was more efficient than the laser after the first treatment, although no further clinical improvement resulted after three treatments. The laser gradually lightened the skin tone and macula and was ultimately more efficient than the IPL after five treatments. CONCLUSION: A series of IPL and nonablative 1,064-nm Q-switched laser treatments were performed with similar efficiency and safety for the improvement in skin texture, pore size, and sebum secretion. IPL was faster, but nonablative 1,064-nm Q-switched laser was more effective in improving skin tone and macula.

[A rhombus shape excision of the soft palate to treat obstructive sleep apnea hypopnea syndrome].

OBJECTIVE: To explore a surgical treatment for obstructive sleep apnea hypopnea syndrome (OSAHS). METHODS: 12 cases were treated during the period from Jan 1998 to Aug 2006. Partial soft palate was resected in rhombus shape from the middle to shorten the soft palate and enlarge the pharyngeal cavity. The uvula was reserved. RESULTS: The patients were followed up for six months to five years. There was no complication. Good results were achieved in 9 patients. 2 cases got some kind of improvement. No improvement happened in one case who received a partial tongue resection later. CONCLUSIONS: A rhombus shape excision of the soft palate from the middle is effective for the treatment of OSAHS with few complication.

[Partial glossectomy assisted with temperature-controlled radiofrequency for treating macroglossia].

OBJECTIVE: To study the clinical efficacy of partial glossectomy assisted with temperature-controlled radiofrequency for treating macroglossia. METHODS: There were 4 patients performed this procedure. We took a rhombus shape incision in the middle of the tongue and performed a wedge excision. RESULTS: The mouth can close entirely in all of patients and there weren' t hemorrhage and obviously swollen; Tongue's sensory function hadn't disturbance. Masticate function were normal. One patient still had the symptom that tongue lied outside the oral cavity occasionally after operation. The symptom was disappeared after Temperature-controlled radiofrequency (TCRF) ablation. All of patients' parent were satisfied with the results. CONCLUSIONS: Partial glossectomy assisted with temperature-controlled radiofrequency for treating macroglossia is an effective, much safer and less invasive procedure without obvious adverse reactions. There are better prospects for applying.

[Treatment of the congenital exstrophy of bladder].

OBJECTIVE: To sum up the methods repairing the congenital bladder exstrophy, and to evaluate the value of the abdominal fascial flap and the lower abdominal skin flap, the tensor fascia lata musculocutaneous flap treating the bladder exstrophy. METHODS: The abdominal rectus sheath and the fascia flap of obliquus externus abdominal and the lower abdominal skin flap, or the tensor fascia lata musculocutaneous flap were used to repair the abdominal wall defection, meanwhile repair the epispadias. The maximum field of the musculocutaneous flaps was 8 cm x 10 cm. RESULTS: 12 cases were cured. The bladder exstrophy and the abdominal wall defection were repaired very well. The musculus sphincter bladder neck was reconstructed. After the second operation, the cases could urinate under self-control. 2 skin flaps of 8 musculocutaneous flaps were necrosis and cured by skin grafting. CONCLUSIONS: Using the abdominal fascial flap and the lower abdominal skin flap, or the tensor fascia lata musculocutaneous flap, is feasible for repairing the bladder exstrophy and the lower abdominal wall defection. The continuous of the musculus sphincter bladder were renewed, and then the patients could urinate under self-control. When the ischemia of the musculocutaneous flaps had occurred, the tensor fascial was survived well.

[The clinical application of acellular urinary bladder submucosa to reconstructive the urethral duct of hypospadias patients].

OBJECTIVE: Attended to apply the acellular urinary bladder submucosa in the operation of the urethral duct reconstruction of penile hypospadias. METHODS: In 2 cases, the urethral ducts were reconstructed by using the acellular urinary bladder submucosa as dorsal half of the urethra, while the preputial island flap as ventral side. In the other patient, the urethral plate was incised, similar to Snodgrass operation, the matrix was fixed to the corpora cavernosa beneath. RESULTS: Uroflowmetry and the urethroscopy were been performed in the one year follow up. A bell-shaped flow curve was obtained in the patient after urethroplasty using matrix graft combined with a local flap, and no fistula, stricture was found after a year or half a year follow-up. CONCLUSIONS: The freeze-dried acellular urinary bladder submucosa had three-dimensional network structure, and the acellular urinary bladder submucosa graft combined with preputial flap for hypospadias repair provided satisfactory functional results.

[An experimental study on the culture of human oral mucosa epithelium using autologous serum].

OBJECTIVE: To investigate the biological properties of cultured human oral mucosa epithelium using autologous serum in order to provide a new material for tissue engineering urethra. METHODS: The cultured oral mucosa epithelium was respectively transplanted beneath the skin, above the deep fascia and in the wound of the athymic mice. The specimens were taken at 2, 3, 4, and 6 weeks posttransplantation, and processed for (1) immunofluorescence anti-HLA staining to determine graft acceptance, and (2) anti-human IV collagen and antihuman laminin immunohistochemical staining procedures to indicate the basement membrane formation. RESULTS: All the grafts survived and grew very well. The grafts were positive to anti-HLA. Collagen type IV and laminin were detected at the dermo-epidermal junction in all groups from day 14, and increasing in density up to day 21. CONCLUSIONS: he cultured human oral mucosa epithelium by autologous serum could develop an excellent functional epithelium tissue, which would be used to reconstruct urethra and repair wound.

[Autologous buccal mucosal graft pre-transplantation for urethral reconstruction of complicated hypospadias].

OBJECTIVE: Applicate autologous buccal mucosal graft pre-transplantation for urethral reconstruction of complicated hypospadias by staging. METHODS: From 1999 to 2003, 40 patients have been treated by method of autologous buccal mucosal graft pre-placing. There were two models: graft onlay and graft-tube for urethral reconstruction, and the 2rd operation was accomplished half year later. RESULTS: All patients healed without urethral fistula. CONCLUSION: The method is valuable for treatment of complicated hypospadias.

[The inherent liability to infection of various tissue for urethra reconstruction].

OBJECTIVE: Observing the long-term results of reconstructed urethra to reduce the postop infection rate of hypospadias. To explore the standard of selecting tissue for urethra reconstruction by comparing the different inherent liability to infection in use of different kinds of tissue for urethra reconstruction. METHODS: Neo-urethra inner wall and normal urethra were observed through urography and urethroscope. The tissue section of neo-urethra, normal urethra and tissue for urethra reconstruction were observed through light microscope, scanning electron microscope and transmission electron microscope. RESULTS: The structure and liability to infection of neo-urethra made of bladder mucosa, buccal mucosa or skin never changed after long time. Metaplasia never took place in neo-urethra. CONCLUSIONS: The structure and liability to infection of neo-urethra were the same as those of the congeneric tissue. Bladder or buccal mucosa is the best tissue for urethra reconstruction considering urethra microenvironment. Neo-urethra made of skin is more liable to infection by nature. The best opportunity of hypospadias operation should be before puberty.

[Bacteriology study of hypospadias].

OBJECTIVE: This clinical study was to explore why the neo-urethra is liable to be infected after hypospadias operation, find the source and the common floras of infection, and accordingly, improve the peroperation procedures so as to reduce postoperative infection rate. METHODS: The pathogenic floras were examined and analyzed by germiculture and karyotype analysis. RESULTS: The bacteria in the neo-urethra mostly came from the orifice and the reconstruction material of the urethra. The most common floras that caused infection were gram-positive coccus. The most sensitive antibiotics for hypospadias infection were demethylvancomycin. CONCLUSION: The postoperative infection of hypospadias is incisional, not the urinary system infection. Because the microenvironment of the neo-urethra is more suitable for infection than that of the skin or mucosa, the reconstructed urethra is likely to be infected.

[Rabbit urethral defect repair with freeze-dried acellular bladder submucosa].

OBJECTIVE: To evaluate the result of urethral defect repair using freeze-dried acellular bladder submucosa. METHODS: The freeze-dried acellular bladder submucosa was obtained from human cadaveric bladder submucosa using freeze-thawed enzymatic treatment and the freeze-drying technique. 18 male New Zealand rabbits were used in this experiment. A defect of 1.0 cm x 0.5 cm was created in the ventral urethra of the animal. The acellular matrix was grafted to the urethral defect in 14 rabbits. The other 4 animals underwent a sham operation as the controls. From postoperative one to twenty-four weeks, the animals underwent retrograde urethrography at certain intervals. The grafts of the acellular matrix were taken and prepared for histological evaluation. RESULTS: No obvious urethral stricture was observed in the 14 matrix-grafted animals. Histological examinations of the specimens showed progressive infiltration of cells and vascular vessels. Complete epithelization was obtained at 2 weeks. The orientation of collagen fibers in the matrix-grafted area tended to be regular with time. CONCLUSIONS: Freeze-dried acellular bladder submucosa may be a suitable material for urethral defect repair, which induces urethral mucous cells to migrate, grow and epithelialize.

[Inner preputial skin grafts combined with preputial island flap for treatment of penile hypospadias].

OBJECTIVE: To investigate new methods of treatment for penile hypospadias. METHODS Urethroplasty was performed for penile hypospadias using inner preputial skin grafts combined with preputial island flap. RESULTS: A total of 10 patients have received this procedure. After a mean follow up of one year, no fistula or urethral stricture was found. All patients obtained excellent cosmetic results. CONCLUSIONS: This method allows for adequate correction of severe chordee. It is a useful procedure for selected cases of primary hypospadias with severe incurvation.