RESUMEN
Grouted soil-concrete interfaces exist in bored piles with post-grouting in pile tip or sides and they have a substantial influence on pile skin friction. To study the effect of grouting volume on the shearing characteristics of the interface between cohesive soil and concrete piles with different roughness, grouting equipment and a direct shear apparatus were combined to carry out a total of 48 groups of direct shear tests on cohesive soil-concrete interfaces incorporating the grouting process. The test results showed that the shear behavior of the grouted cohesive soil-concrete interface was improved mainly because increasing the grouting volume and roughness increased the interfacial apparent cohesion. In contrast, increasing the grouting volume and roughness had no obvious increasing effects on the interfacial friction angle. Interfacial grouting contributed to the transition in the grouted cohesive soil from shrinkage to dilation: as the grouting volume increased, the shrinkage became weaker and the dilation became more obvious. The shear band exhibited a parabolic distribution rather than a uniform distribution along the shearing direction and that the shear band thickness was greater in the shearing direction, and it will become thicker with increasing grouting volume or roughness. The analysis can help to understand the shear characteristics of soil-pile interface in studying the vertical bearing properties of pile with post-grouting in tip or sides.
RESUMEN
OBJECTIVE: To investigate the association of human papillomavirus (HPV) in the pubic hair follicles of males with HPV infection in their female sexual partners. METHODS: We included in this study 21 female patients with HPV infection, including 8 cases of cervical cancer, 5 cases of atypical cervical hyperplasia, 5 cases of cervical condyloma, and 3 cases with unidentified causes. We also enlisted 52 men without visible condyloma acuminatum in the external genitalia as healthy controls. We detected HPV in the pubic hair follicles of the female patients' male sexual partners and the healthy male controls by PCR and reverse hybridization in situ. RESULTS: HPV positive was found in 6 (28.6%) of the 21 women's male partners, in whom the HPV types were correspondent situ. to those of the female patients. CONCLUSION: HPV in the pubic hair follicles of men might be one of the causes of HPV-related cervical lesions in their female sexual partners.
Asunto(s)
Folículo Piloso/virología , Infecciones por Papillomavirus/transmisión , Infecciones por Papillomavirus/virología , Enfermedades del Cuello del Útero/virología , Adolescente , Adulto , Estudios de Casos y Controles , Femenino , Genitales Masculinos/virología , Humanos , Masculino , Persona de Mediana Edad , Papillomaviridae , Parejas Sexuales , Neoplasias del Cuello Uterino/virología , Adulto JovenRESUMEN
OBJECTIVE: Human papillomavirus (HPV) commonly exists in healthy individuals, but its prevalence in the pubic hair follicles is not yet clear, nor is the relationship between HPV infection in the pubic hair follicles and the recurrence of genital warts in men. This study aimed to investigate HPV infection in the pubic hair follicles of healthy men and patients with genital warts, and to look into the correlation of HPV infection with recurrent genital warts. METHODS: We included in this study 122 healthy men aged 21-80 years and 86 male patients with genital warts aged 24-61 years, detected HPV in their pubic hair follicles by PCR, and made comparative analysis of the data obtained from the two groups. RESULTS: The positive rate of HPV in the pubic hair follicles of the healthy males was 17.21% (21/122), including 15 cases of HPV6, 4 HPV11, 1 non-HPV6/11 and 1 the mixed type (both HPV6 and HPV11), while that of the genital wart patients was 32.55% (28/86), including 17 cases of HPV6, 7 HPV11, 2 non-HPV6/11 and 2 the mixed type. CONCLUSION: The incidence of HPV infection is higher in patients with genital warts than in healthy men, while the types of HPV involved are basically the same in the two groups, mainly HPV6 and HPV11.
Asunto(s)
Condiloma Acuminado/virología , Folículo Piloso/virología , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/epidemiología , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Condiloma Acuminado/epidemiología , Humanos , Incidencia , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
A pot experiment was conducted to study the impact of Cu and Zn pollution on soil enzyme activities and rape seedlings growth. The results showed that Cu had a stronger inhibitory effect than Zn on soil urease activity, while Zn had more obvious impact on soil calatase activity. The damage on the growth and dry mass of rape was more serious under Cu than under Zn pollution. The inhibition of rape seedlings growth was mainly due to the inhibition of root growth and its material accumulation. Factor analysis indicated that root dry mass was more sensitive than other indices, which could be adopted to monitor soil Cu and Zn pollution.
Asunto(s)
Brassica rapa/crecimiento & desarrollo , Cobre/análisis , Contaminantes del Suelo/análisis , Ureasa/metabolismo , Zinc/análisis , Catalasa/metabolismo , Ecosistema , Raíces de Plantas/crecimiento & desarrollo , Plantones/crecimiento & desarrolloRESUMEN
The study on the Cu forms in Cynodon dactylon rhizosphere soil of copper tailings yard in Tongling City, Anhui Province showed that among the test Cu forms, the amount of residual form occupied the majority, while that of exchangeable form was relatively low. Compared with non-rhizosphere soil, rhizosphere soil had a higher organic matter content but a lower pH. With the growth of C. dactylon, the contents of organically combined and exchangeable Cu in rhizosphere soil increased by 7.89% and 5%, respectively, while those of carbonate-combined and Fe-Mn oxides-combined Cu decreased. The growth of C. dactylon accelerated the transformation of Cu forms in rhizosphere soil, and decreased the rhizosphere soil Cu content through its absorption.
Asunto(s)
Cobre/metabolismo , Cynodon/metabolismo , Minería , Rhizobium/metabolismo , Biodegradación Ambiental , Cynodon/fisiología , Monitoreo del Ambiente , Residuos Industriales/análisis , Rhizobium/fisiología , Contaminantes del Suelo/análisis , Contaminantes del Suelo/metabolismoRESUMEN
Pot-culture experiments were used to examine the individual and combined effects of Cu and Cd pollutants on Trifolium repens L. seedlings, both on their growth and their active oxygen metabolism system, mainly superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. The results showed that the negative action took place at low concentrations of Cu (less than 500 ppm) and Cd (less than 0.5 ppm), which had no obvious effects on the seedlings' growth. However, as the concentrations of Cu and Cd increased (500-3000 ppm and 0.5-50 ppm respectively), synergistic activities was observed, showing obvious negative effects (P less than 0.05). Compared with the control samples, the seedlings affected by Cu and Cd pollutants were shorter and smaller, their fresh/dry weight and content of soluble protein decreased drastically, their leaf electric conductivity increased, and the contents of their leaf pigments decreased. Chlorophyll a was more sensitive than chlorophyll b to Cu and Cd pollutants, and chlorophyll b was more sensitive than carotenoid. It was also shown that the active oxygen metabolism of T. repens seedlings was destroyed by high amounts of Cu and Cd, the balance of the anti-oxidase system was broken, and the CAT and SOD activities noticeably decreased while POD activity evidently increased. Cd had a more noticeable effect on seedling growth than Cu.
Asunto(s)
Cadmio/toxicidad , Cobre/toxicidad , Oxígeno/metabolismo , Biodegradación Ambiental , Biomasa , Catalasa/efectos de los fármacos , Catalasa/metabolismo , Relación Dosis-Respuesta a Droga , Interacciones Farmacológicas , Peroxidasa/efectos de los fármacos , Peroxidasa/metabolismo , Eliminación de Residuos , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Superóxido Dismutasa/efectos de los fármacos , Superóxido Dismutasa/metabolismo , Trifolium/efectos de los fármacos , Trifolium/crecimiento & desarrolloRESUMEN
Using one or two primers respectively bound to the surface of Au nanoparticles (AuNPs) or magnetic nanoparticles (MNPs), polymerase chain reaction (PCR) based on nanoparticles was systemically studied, agarose gel electrophoresis and atomic force microscopy (AFM) were respectively used to detect and observe the PCR product. The results obtained indicated that with either one or two primers respectively bound to the nanoparticle surface, PCR can proceed successfully under optimized condition and is subject to certain rules, consequently a symmetric PCR technique and an asymmetric PCR technique based on nanoparticles have been developed. A kind of nanostructured aggregates can be constructed by a symmetric PCR using two nanoparticle-bound primers.