[Prognostic values of chemokines in different clinical outcomes of influenza A (H1N1)-infected patients].

OBJECTIVE: To analyze the prognostic values of chemokines in different clinical outcomes of influenza A (H1N1)-infected patients. METHODS: A total of 60 cases with influenza A (H1N1) virus were enrolled. There were 32 mild cases, 16 healing cases from severe stage and 12 mortality cases resulting from severe stage. The serum levels of chemokines including CXCL8, CCL10, CCL2, CCL3, CCL4, CCL5 and CCL11 were detected by the Luminex technique. RESULTS: The levels of CXCL8, CCL2 and CCL10 in the mortality group were higher than those of the healing serve and mild cases. And the differences were significant. P value was 0.001, < 0.0001 and 0.027 respectively. The thresholds of CCL2 and CXCL8 for predicting clinical mortality were 45.99 ng/L (sensitivity 100%, specificity 89.58%) and 59.75 ng/L (sensitivity 75%, specificity 95.83%). CONCLUSION: The serum levels of chemokines are elevated in severe cases of influenza A (H1N1). And the rises of CXCL8 and CCL2 are more obvious in the mortality cases. Thus CXCL8 and CCL2 may serve as two prognostic indices for the clinical fatal outcomes.

[The analysis of effect of Th1/Th2 cytokine in the different prognosis in severe influenza A (H1N1)].

OBJECTIVE: To explore the effect of cytokine in the different prognosis of patients with severe influenza A (H1N1) infection. METHODS: 28 cases with severe influenza A (H1N1) were enrolled in the study including 16 cured cases and 12 dead cases. The cytokine level in serum was detected by Luminex technology. RESULTS: The levels of IL-2, IL-12 (P70) and IFN-gamma in dead group was lower than cured and normal control group and the difference were significant, P <0.05, respectively. IL-4 level in the dead group was significantly lower than cured group and normal control group, P value was 0.0310 and 0.0012, respectively. CONCLUSIONS: The Thl cytokine level in the severe 2009 epidemic H1N1 influeaze cases shows decreased trend, and the trend is more obvious in dead cases. The decrease of Th1 cytokine may be one of reasons leading to severe clinical situation and related withthe bad prognosis.