Asunto(s)
Conducta de Elección , Toma de Decisiones , Seguridad del Paciente , Parto Vaginal Después de Cesárea/ética , Parto Vaginal Después de Cesárea/legislación & jurisprudencia , Consejo , Documentación , Femenino , Humanos , Consentimiento Informado , Partería/ética , Partería/legislación & jurisprudencia , Complicaciones del Trabajo de Parto , Aceptación de la Atención de Salud , Autonomía Personal , Relaciones Médico-Paciente , Embarazo , Atención Primaria de Salud , Reino UnidoRESUMEN
Serpula lacrymans, the causative agent of dry rot timber decay in buildings, is a Basidiomycete fungus in the Boletales clade. It owes its destructiveness to a uniquely well-developed capacity to colonize by rapid mycelial spread from sites of initial spore infection, coupled with aggressive degradation of wood cellulose. Genomic methods have recently elucidated the evolution and enzymic repertoire of the fungus, suggesting that it has a distinctive mode of brown rot wood decay. Using novel methods to image nutrient translocation, its mycelium has been modeled as a highly responsive resource-supply network. Dry rot is preventable by keeping timber dry. However, in established outbreaks, further mycelial spread can be arrested by inhibitors of translocation.
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Basidiomycota , Madera , Basidiomycota/metabolismo , Hongos , Lignina/metabolismo , MicelioRESUMEN
The herpes zoster virus is a common neurocutaneous infection. When the eye is involved, the virus is referred to as herpes zoster ophthalmicus. This article discusses the role of the nurse in managing the care of patients with herpes zoster ophthalmicus. It provides an overview of the condition and its associated pathophysiology. The article examines risk factors, clinical features, diagnosis, treatment, complications and prevention. It also discusses the care required to support patients during their treatment and subsequent rehabilitation.
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Herpes Zóster Oftálmico/enfermería , Herpes Zóster Oftálmico/diagnóstico , Herpes Zóster Oftálmico/fisiopatología , Humanos , Rol de la Enfermera , Factores de Riesgo , Reino UnidoRESUMEN
Transport networks are vital components of multi-cellular organisms, distributing nutrients and removing waste products. Animal cardiovascular and respiratory systems, and plant vasculature, are branching trees whose architecture is thought to determine universal scaling laws in these organisms. In contrast, the transport systems of many multi-cellular fungi do not fit into this conceptual framework, as they have evolved to explore a patchy environment in search of new resources, rather than ramify through a three-dimensional organism. These fungi grow as a foraging mycelium, formed by the branching and fusion of threadlike hyphae, that gives rise to a complex network. To function efficiently, the mycelial network must both transport nutrients between spatially separated source and sink regions and also maintain its integrity in the face of continuous attack by mycophagous insects or random damage. Here we review the development of novel imaging approaches and software tools that we have used to characterise nutrient transport and network formation in foraging mycelia over a range of spatial scales. On a millimetre scale, we have used a combination of time-lapse confocal imaging and fluorescence recovery after photobleaching to quantify the rate of diffusive transport through the unique vacuole system in individual hyphae. These data then form the basis of a simulation model to predict the impact of such diffusion-based movement on a scale of several millimetres. On a centimetre scale, we have used novel photon-counting scintillation imaging techniques to visualize radiolabel movement in small microcosms. This approach has revealed novel N-transport phenomena, including rapid, preferential N-resource allocation to C-rich sinks, induction of simultaneous bi-directional transport, abrupt switching between different pre-existing transport routes, and a strong pulsatile component to transport in some species. Analysis of the pulsatile transport component using Fourier techniques shows that as the colony forms, it self-organizes into well demarcated domains that are identifiable by differences in the phase relationship of the pulses. On the centimetre to metre scale, we have begun to use techniques borrowed from graph theory to characterize the development and dynamics of the network, and used these abstracted network models to predict the transport characteristics, resilience, and cost of the network.
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Alimentos , Hongos/metabolismo , Procesamiento de Imagen Asistido por Computador/métodos , Microscopía Confocal/métodos , Microscopía Fluorescente/métodos , Microscopía por Video/métodos , Micelio/metabolismoRESUMEN
Saprotrophic woodland fungi forage for mineral nutrients and woody resources by extension of a mycelial network across the forest floor. Different species explore at different rates and establish networks with qualitatively differing architecture. However, detailed understanding of fungal foraging behaviour has been hampered by the absence of tools to quantify resource allocation and growth accurately and non-invasively. To solve this problem, we have used photon-counting scintillation imaging (PCSI) to map and quantify nutrient allocation and localised growth simultaneously in heterogeneous resource environments. We show that colonies spontaneously shift to an asymmetric growth pattern, even in the absence of added resources, often with a distinct transition between the two growth phases. However, the extent of polarisation was much more pronounced and focussed in the presence of an additional cellulose resource. In this case, there was highly localised growth, often at the expense of growth elsewhere in the colony, and marked accumulation of (14)C-AIB in the sector of the colony with the added resource. The magnitude of the response was greatest when resource was added around the time of the endogenous developmental transition. The focussed response required a metabolisable resource, as only limited changes were seen with glass fibre discs used to mimic the osmotic and thigmotropic stimuli upon resource addition. Overall the behaviour is consistent with an adaptive foraging strategy, both to exploit new resources and also to redirect subsequent foraging effort to this region, presumably with an expectation that the probability of finding additional resources is increased.
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Modelos Estadísticos , Phanerochaete/citología , Phanerochaete/crecimiento & desarrollo , Transporte Biológico , Radioisótopos de Carbono/metabolismo , Cámaras gamma , Hifa/química , Hifa/citología , Hifa/crecimiento & desarrollo , Hifa/fisiología , Modelos Biológicos , Phanerochaete/química , Phanerochaete/fisiologíaRESUMEN
Diabetes is a progressive disease that may adversely affect healthy functioning of the body. The eyes are often indicators of systemic disease and are particularly vulnerable to the pathophysiological changes that occur as a result of diabetes. This article provides an overview of five main ocular complications associated with diabetes, cataract, diabetic retinopathy, central retinal vein occlusion, central retinal artery occlusion and neovascular glaucoma. The assessment and treatment of such complications and the role of the nurse in the care and management of patients are discussed.
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Catarata/terapia , Complicaciones de la Diabetes/terapia , Retinopatía Diabética/terapia , Glaucoma Neovascular/terapia , Rol de la Enfermera , Oclusión de la Arteria Retiniana/terapia , Catarata/diagnóstico , Catarata/etiología , Extracción de Catarata/enfermería , Complicaciones de la Diabetes/diagnóstico , Complicaciones de la Diabetes/etiología , Retinopatía Diabética/clasificación , Retinopatía Diabética/diagnóstico , Retinopatía Diabética/etiología , Glaucoma Neovascular/diagnóstico , Glaucoma Neovascular/etiología , Humanos , Terapia por Láser/enfermería , Evaluación en Enfermería , Educación del Paciente como Asunto , Atención Perioperativa/métodos , Atención Perioperativa/enfermería , Oclusión de la Arteria Retiniana/diagnóstico , Oclusión de la Arteria Retiniana/etiología , Selección Visual , Vitrectomía/enfermeríaRESUMEN
Fungi play a central role in the nutrient cycles of boreal and temperate forests. In these biomes, the saprotrophic wood-decay fungi are the only organisms that can completely decompose woody plant litter. In particular, cord-forming basidiomycete fungi form extensive mycelial networks that scavenge scarce mineral nutrients and translocate them over long distances to exploit new food resources. Despite the importance of resource allocation, there is limited information on nutrient dynamics in these networks, particularly for nitrogen, as there is no suitable radioisotope available. We have mapped N-translocation using photon-counting scintillation imaging of the non-metabolised amino acid analogue, (14)C-aminoisobutyrate. We describe a number of novel phenomena, including rapid, preferential N-resource allocation to C-rich sinks, induction of simultaneous bi-directional N-transport, abrupt switching between different pre-existing transport routes, and emergence of locally synchronised, oscillatory phase domains. It is possible that such self-organised oscillatory behaviour is a mechanism to achieve global co-ordination in the mycelium.
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Transporte Biológico , Hongos/fisiología , Micelio/crecimiento & desarrollo , Relojes Biológicos/fisiología , Radioisótopos/metabolismoRESUMEN
Microorganisms display a range of oscillatory phenomena that operate over different temporal scales. Fourier analysis provides a compact description of such oscillations in terms of their frequency, magnitude and phase. However, in the majority of studies there is no explicit consideration of the spatial organisation of the oscillation. Here we describe procedures and a software package to map oscillatory phenomena in microorganisms in both the time and frequency domains. Key parameters of interest, such as frequency, phase or magnitude of the oscillations, are presented as pseudo-colour coded maps. This maintains the spatial information in the image and greatly facilitates understanding of potentially complex propagating waves or development of oscillatory domains with distinct behaviour. We illustrate the utility of this system with reference to spatial analysis of the pulsatile component to amino acid transport in mycelial systems of Phanerochaete velutina and Coniophora puteana, and actin-myosin based contractions in Physarum polycephalum.
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Análisis de Fourier , Hongos/fisiología , Actinas/metabolismo , Relojes Biológicos/fisiología , Hongos/citología , Miosinas/metabolismoRESUMEN
Mycelial fungi have a growth form which is unique among multicellular organisms. The data presented here suggest that they have developed a unique solution to internal solute translocation involving a complex, extended vacuole. In all filamentous fungi examined, this extended vacuole forms an interconnected network, dynamically linked by tubules, which has been hypothesized to act as an internal distribution system. We have tested this hypothesis directly by quantifying solute movement within the organelle by photobleaching a fluorescent vacuolar marker. Predictive simulation models were then used to determine the transport characteristics over extended length scales. This modeling showed that the vacuolar organelle forms a functionally important, bidirectional diffusive transport pathway over distances of millimeters to centimeters. Flux through the pathway is regulated by the dynamic tubular connections involving homotypic fusion and fission. There is also a strongly predicted interaction among vacuolar organization, predicted diffusion transport distances, and the architecture of the branching colony margin.
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Basidiomycota/metabolismo , Basidiomycota/fisiología , Transporte Biológico/fisiología , Vacuolas/fisiología , Basidiomycota/ultraestructura , Comunicación Celular , Compartimento Celular , Difusión , Eficiencia/fisiología , Fluoresceínas/farmacología , Técnica del Anticuerpo Fluorescente , Hifa/crecimiento & desarrollo , Hifa/fisiología , Modelos Biológicos , Transducción de SeñalRESUMEN
This article examines the paediatric ophthalmic nurse's role in treating and managing several common ocular conditions.
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Oftalmopatías , Catarata/congénito , Catarata/enfermería , Catarata/fisiopatología , Catarata/terapia , Niño , Oftalmopatías/diagnóstico , Oftalmopatías/enfermería , Oftalmopatías/fisiopatología , Oftalmopatías/terapia , Glaucoma/congénito , Glaucoma/diagnóstico , Glaucoma/enfermería , Glaucoma/terapia , Humanos , Retinoblastoma/diagnóstico , Retinoblastoma/enfermería , Retinoblastoma/terapia , Estrabismo/enfermería , Estrabismo/fisiopatología , Estrabismo/terapiaRESUMEN
The subject for the Nicholas Research Award 1983 was prompted by the author's earlier involvement in quality assurance and her realization that there was a need to investigate the economic aspects of the subject. In a comparative study, the paper shows that the running costs of a basic quality assurance programme are probably less than 1% of the overall running costs of an 'average' five roomed X-ray department. Future professional implications associated with quality assurance are also discussed.
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Garantía de la Calidad de Atención de Salud/economía , Radiografía/economía , Análisis Costo-Beneficio , Humanos , Garantía de la Calidad de Atención de Salud/tendencias , Servicio de Radiología en Hospital/economía , Servicio de Radiología en Hospital/normas , Reino UnidoRESUMEN
A reject analysis has been undertaken for a 26 week period in the radiology department of an oncological centre. The overall reject and repeat rates are presented here, as well as those arising from individual examinations and X-ray rooms. The variations in reject rates for different examinations as a function of time are highlighted and the role of reject analysis in quality assurance is discussed.