RESUMEN
Cells with a resemblance to Gaucher cells, sometimes called pseudo-Gaucher cells, are seen in the bone marrow of some patients with hematologic malignancy or anemia. These cells are derived from cells of the monocytic lineage but do not show the characteristic inclusions of true Gaucher cells when examined by electron microscopy. Large numbers of Gaucher-like cells were found in the livers at autopsy of 2 patients with hematologic malignancy treated with chemotherapy and bone marrow transplant. Knowledge of this phenomenon may be useful in the interpretation of liver biopsy done on a patient with bone marrow transplant.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Trasplante de Médula Ósea , Histiocitos/patología , Leucemia Prolinfocítica/patología , Hígado/patología , Leucemia-Linfoma Linfoblástico de Células Precursoras/patología , Anciano , Autopsia , Terapia Combinada , Resultado Fatal , Enfermedad de Gaucher/diagnóstico , Humanos , Cuerpos de Inclusión/ultraestructura , Macrófagos del Hígado/patología , Leucemia Prolinfocítica/terapia , Masculino , Persona de Mediana Edad , Leucemia-Linfoma Linfoblástico de Células Precursoras/terapiaRESUMEN
PAX2 plays an important role in kidney development; although small studies have demonstrated PAX2 expression in Wilms tumors (WT), comprehensive studies on formalin-fixed tissue are lacking. Thus, we systematically evaluated PAX2 immunohistochemical staining in a retrospective study of pediatric WT, as compared with other pediatric tumors. We stained formalin-fixed, paraffin-embedded sections from 39 WT, 6 nephrogenic rests, 8 non-Wilms renal tumors, and 43 nonrenal pediatric small round cell tumors with 2 different PAX2 polyclonal antibodies. PAX2 demonstrated strong, diffuse staining of epithelial and blastema components of WT (97% of cases). PAX2 stained WT stroma in fewer cases (23%), but 80% of anaplastic foci were positive. Nephrogenic rests, 1 case of metanephric adenoma, and 1 pediatric renal cell carcinoma were also PAX2 positive; other pediatric renal tumors were negative. Neuroblastoma, primitive neuroectodermal tumor/Ewings, and T-cell acute lymphoblastic lymphoma (ALL) were PAX2 negative. However, PAX2 weakly stained some cases of B-cell ALL rhabdomyosarcoma (RMS) was also stained, especially alveolar RMS (83%), with less staining of embryonal RMS (13%). One of the antibodies also stained maturing myoid cytoplasm of WT and RMS. This study shows that PAX2 is a sensitive marker of WT (sensitivity 97%), but PAX2 shows weak-to-moderate-intensity nuclear staining of RMS and B-cell ALL, somewhat limiting its utility. However, PAX2 may be a helpful marker in certain diagnostic situations. We speculate that RMS and B-cell ALL staining could be due to antibody cross-reactivity with PAX family members with known expression in RMS and B-cell ALL.
Asunto(s)
Biomarcadores de Tumor/análisis , Neoplasias Renales/química , Factor de Transcripción PAX2/análisis , Tumor de Wilms/química , Adolescente , Neoplasias Óseas/química , Neoplasias Encefálicas/química , Niño , Preescolar , Células Epiteliales/química , Humanos , Inmunohistoquímica , Neoplasias Renales/patología , Neuroblastoma/química , Tumores Neuroectodérmicos Primitivos/química , Leucemia-Linfoma Linfoblástico de Células Precursoras/metabolismo , Valor Predictivo de las Pruebas , Estudios Retrospectivos , Rabdomiosarcoma/química , Sarcoma de Ewing/química , Células del Estroma/química , Tumor de Wilms/patologíaRESUMEN
OBJECTIVE: To determine the event-free survival (EFS) and overall survival (OS) of children with very low risk Wilms tumor (VLRWT) treated with surgery only. BACKGROUND: Previous studies suggested that postoperative chemotherapy had not improved the prognosis of children with VLRWT. A total of 77 children <24 months of age with small (<550 g) Stage I favorable histology Wilms tumors were treated with surgery only. This study was closed based on stopping rules to ensure that the 2-year EFS was > or =90%. METHODS: A total of 77 children were assessed for EFS and OS. Of these patients, 21 enrolled at the time of closure were recalled, treated with dactinomycin and vincristine (regimen EE4A), and censored for analysis thereafter. About 111 children subsequently treated with EE4A were available for comparison. RESULTS: Median follow-up of surviving patients was 8.2 years for surgery only (range, 1.9-11.8 years) and 5.2 years for the EE4A group (range, 1.6-8.9 years). The estimated 5-year EFS for surgery only was 84% (95% confidence interval [CI]: 73%, 91%); for the EE4A patients it was 97% (95% CI: 92%, 99%, P = 0.002). One death was observed in each treatment group. The estimated 5-year OS was 98% (95% CI: 87%, 99%) for surgery only and 99% (95% CI: 94%, 99%) for EE4A (P = 0.70). CONCLUSION: The surgery-only EFS was lower than anticipated but, coupled with a much higher than anticipated salvage rate of the chemotherapy naive patients whose disease recurred, led to an observed long-term OS equivalent to that seen with 2-drug chemotherapy. This approach to the treatment of patients with VLRWT eliminates the toxic side-effects of chemotherapy for a large majority of patients. A follow-up study is underway to confirm these findings.
Asunto(s)
Neoplasias Renales/cirugía , Tumor de Wilms/cirugía , Humanos , Lactante , Neoplasias Renales/mortalidad , Factores de Riesgo , Tasa de Supervivencia , Factores de Tiempo , Resultado del Tratamiento , Tumor de Wilms/mortalidadRESUMEN
Both maternal glucocorticoid administration and maternal dietary protein or food restriction in pregnancy cause fewer nephrons and hypertension in the adult offspring. The purpose of these studies was to determine the extent to which nutritional factors contribute to programming of offspring hypertension by maternal glucocorticoids. Pregnant rats were treated with dexamethasone (100 microg x kg(-1) x d(-1) sc) on days 1-10 (ED) or days 15-20 (LD) of pregnancy. Additional groups of pregnant animals were pair fed to the early (EDPF) and late (LDPF) dexamethasone-treated groups, and another group was untreated or given vehicle (C). The dams treated with dexamethasone reduced their food intake and lost or failed to gain a normal amount of weight during treatment; body weights of ED dams caught up to normal after the treatment period, whereas those of LD dams did not. In adulthood ( approximately 21 wks), chronically instrumented male offspring of ED had normal blood pressures (125 +/- 2 mmHg vs. 126 +/- 1 mmHg in C), whereas LD offspring were hypertensive (136 +/- 3 mmHg). However, LDPF offspring were equally hypertensive (134 +/- 2 mmHg). Glomerular filtration rates normalized to body weight were not significantly different among groups. Qualitatively similar results were found in female offspring. Thus the long-term effects of maternal glucocorticoid administration at this dose on offspring's blood pressure may, in large part, be accounted for by the reduction in maternal food intake. These data suggest that maternal glucocorticoids and maternal food or protein restriction may, at least in part, share a common mechanism in programming offspring for hypertension. The window of sensitivity of future offspring blood pressure to either maternal insult coincides with nephrogenesis in the rat, suggesting that impaired renal development could play an important role in this programming.
Asunto(s)
Presión Sanguínea/efectos de los fármacos , Peso Corporal/efectos de los fármacos , Dexametasona/farmacología , Ingestión de Alimentos/efectos de los fármacos , Riñón/embriología , Riñón/fisiología , Efectos Tardíos de la Exposición Prenatal , Corticoesteroides/administración & dosificación , Animales , Femenino , Masculino , Exposición Materna , Embarazo , Preñez , Ratas , Ratas Sprague-DawleyRESUMEN
In humans, infants who are born small have been reported to have higher blood pressure in adulthood than do larger infants. This suggests that factors in the intrauterine environment that affect fetal growth can program the individual for hypertension later in life. The present study determined whether there is a similar, naturally occurring relationship between birth weight and adult blood pressure in rats. Female Sprague-Dawley rats bred in our colony were fed a normal diet during pregnancy. On the day of delivery, any pups that weighed <90% of the mean pup weight for the litter were identified as runts. For each runt, a sex-matched littermate of normal weight was also identified and assigned to this study. These pairs were chronically instrumented at approximately 20 wk of age. Mean arterial pressure was significantly higher in runt male and female offspring compared with their normal birth weight littermates (males: 149 +/- 7, runts versus 129 +/- 4 mmHg, controls; females: 128 +/- 1, runts versus 119 +/- 2 mmHg, controls). Although the runts had smaller body weights at study than did their littermate controls, the kidney-to-body weight ratio and renal function normalized to kidney or body weight were not different. These studies indicate that adult blood pressure is related to birth weight in rats, as it is in humans. The relative hypertension in runt animals is not due to gross differences in renal function but may be related to more subtle renal structural and/or functional differences.
Asunto(s)
Presión Sanguínea/fisiología , Retardo del Crecimiento Fetal/patología , Retardo del Crecimiento Fetal/fisiopatología , Hipertensión/patología , Hipertensión/fisiopatología , Glándulas Suprarrenales/patología , Factores de Edad , Animales , Peso al Nacer , Femenino , Riñón/patología , Riñón/fisiología , Masculino , Miocardio/patología , Tamaño de los Órganos , Embarazo , Ratas , Ratas Sprague-DawleyRESUMEN
BACKGROUND: Modest maternal protein restriction leads to hypertension and a reduced number of glomeruli in adult male but not female offspring. This study determined whether a more severe protein restriction has equivalent effects on male and female rat offspring, and examined the role of nephrogenesis in this programming. METHODS: Sprague-Dawley rats were fed a protein-restricted (5% protein) diet throughout (LLP), or during the first (LLP/NP) or second (NP/LLP) half of pregnancy. Controls ate a normal diet (NP, 19% protein). Adult offspring were chronically instrumented at 22 weeks; glomerular number and volume were estimated using stereologic techniques. RESULTS: Mean arterial pressures in male offspring were significantly higher in LLP (136 +/- 2 mm Hg) or NP/LLP (137 +/- 2 mm Hg) than in LLP/NP (125 +/- 1 mm Hg) or NP (125 +/- 2 mm Hg). Moreover, the hypertension was salt-sensitive (increase of 16 +/- 4 mm Hg in LLP on a high Na(+) diet compared to 2 +/- 2 mm Hg in NP). Glomerular number (per kidney) was reduced (15,400 +/- 2,411 in LLP vs. 27,208 +/- 1,534 in NP) but average individual glomerular volume was not different (1.98 +/- 0.18 106 micro(3) in LLP vs. 2.01 +/- 0.14 106 micro(3) in NP). Female offspring showed qualitatively similar results. CONCLUSION: Severe maternal dietary protein restriction reduces glomerular number and programs for salt-sensitive adult hypertension in both female and male offspring. The window of sensitivity of adult blood pressure to prenatal protein restriction falls within the period of nephrogenesis in the rat. These data are consistent with the hypothesis that maternal protein restriction causes adult hypertension in the offspring through impairment of renal development.
Asunto(s)
Presión Sanguínea , Dieta con Restricción de Proteínas , Riñón/embriología , Efectos Tardíos de la Exposición Prenatal , Envejecimiento , Animales , Desarrollo Embrionario y Fetal , Femenino , Tasa de Filtración Glomerular , Crecimiento , Riñón/patología , Glomérulos Renales/patología , Masculino , Embarazo , Ratas , Ratas Sprague-Dawley , Circulación Renal , Caracteres SexualesRESUMEN
We describe the case of a testicular juvenile granulosa cell tumor (JGCT) in a 4-year-old boy. The highly undifferentiated appearance and robust mitotic activity of the neoplasm led to an initial impression of an aggressive, small round cell tumor of childhood. Immunocytochemical and ultrastructural studies excluded the usual members of that group, and led to the correct diagnosis. To our knowledge, this is the oldest reported patient to present with this tumor in the testis, and the first with clinical evidence of hormonal activity. The benign behavior of testicular JGCT mandates that it be distinguished from other, much more aggressive, neoplasms which it may resemble.
Asunto(s)
Carcinoma de Células Pequeñas/patología , Tumor de Células de la Granulosa/patología , Neoplasias Testiculares/patología , Carcinoma de Células Pequeñas/ultraestructura , Preescolar , Diagnóstico Diferencial , Tumor de Células de la Granulosa/ultraestructura , Humanos , Inmunohistoquímica , Masculino , Microscopía Electrónica , Neoplasias Testiculares/ultraestructuraRESUMEN
Electron microscopy was used to examine 72 cases of medulloblastoma to better characterize the ultrastructural spectrum of this tumor. Twenty-four cases showed prominent neural differentiation. Twenty-three cases showed minimal (21) or no (2) recognizable neural differentiation, and the remainder of the cases (25) showed intermediate differentiation. All 42 cases tested stained for neuron-specific enolase, 28 for synaptophysin, and 12 for neurofilament protein. All cases showed strong reactivity for glial fibrillary acidic protein (GFAP) within reactive astrocytes. Three cases showed reactivity for GFAP within tumor cells. Medulloblastoma exhibits a broad spectrum of neural differentiation, with nearly all cases showing at least some degree of this change, and it universally exhibits participation of reactive astrocytes which can create a potential for diagnostic confusion.
Asunto(s)
Biomarcadores de Tumor/análisis , Neoplasias Cerebelosas/patología , Neoplasias Cerebelosas/ultraestructura , Meduloblastoma/patología , Meduloblastoma/ultraestructura , Astrocitos/patología , Astrocitos/ultraestructura , Diferenciación Celular , Neoplasias Cerebelosas/metabolismo , Niño , Humanos , Inmunohistoquímica , Meduloblastoma/metabolismo , Microscopía Electrónica , Neuronas/patología , Neuronas/ultraestructuraRESUMEN
A 4-year-old boy with muscle weakness underwent skeletal muscle biopsies. Light microscopy showed distinct eosinophilic inclusions within the majority of muscle cell nuclei, but none in the cytoplasm. Electron microscopy revealed crystalline, round to rod-shaped inclusions in the muscle cell nuclei. The inclusions stained positively for alpha-actinin. Intranuclear inclusions identical to those seen here have been described in rare cases of nemaline rod myopathy, though almost always together with classic intracytoplasmic rods. This case illustrates the importance of electron microscopy in the diagnosis of rare myopathies and in the characterization of cellular inclusions of unknown origin.
Asunto(s)
Núcleo Celular/ultraestructura , Cuerpos de Inclusión Intranucleares/ultraestructura , Músculo Esquelético/patología , Miopatías Nemalínicas/patología , Biomarcadores/análisis , Preescolar , Cristalización , Humanos , Técnicas para Inmunoenzimas , Masculino , Debilidad Muscular/etiología , Debilidad Muscular/metabolismo , Debilidad Muscular/patología , Músculo Esquelético/metabolismo , Miopatías Nemalínicas/complicaciones , Miopatías Nemalínicas/metabolismoRESUMEN
A 3-month-old girl with congenital secretory diarrhea underwent a duodenal biopsy. Histologic study showed villous atrophy and large amounts of PAS-positive material within enterocyte cytoplasm. Despite a clinical suspicion of microvillous inclusion disease, 2 sessions of electron microscopy were unsuccessful in detecting the diagnostic inclusions. Instead, large aggregates of electron-lucent, vermiform membranous vesicles were observed in enterocyte cytoplasm, corresponding to the PAS-positive material. A third attempt at electron microscopy was successful in detecting small numbers of microvillous inclusions. These and other recently reported cases document an expanding spectrum of ultrastructural findings in this disease, including examples where the classic microvillous inclusions are overshadowed by other features.
Asunto(s)
Vesículas Citoplasmáticas/ultraestructura , Diarrea Infantil/patología , Enterocitos/ultraestructura , Enfermedades Intestinales/patología , Microvellosidades/ultraestructura , Diarrea Infantil/congénito , Femenino , Humanos , Cuerpos de Inclusión/ultraestructura , Lactante , Enfermedades Intestinales/complicaciones , Reacción del Ácido Peryódico de SchiffRESUMEN
A 17-year-old boy presented with a history of longstanding hematuria and non-nephrotic proteinuria without renal insufficiency, for which renal biopsy was performed. The findings by routine light microscopy and direct immunofluorescence study were mild and nonspecific. Electron microscopy, however, demonstrated the unexpected finding of distinct collagen fibrils within capillary wall basement membranes, typical of the nail-patella syndrome. Repeat physical examination following the biopsy confirmed the presence of normal nails and patellae, and radiographs of the knees were also normal. The boy's renal disease was stable at last follow-up. The authors briefly discuss the differential diagnosis, and suggest that this case represents an unusual manifestation of the nail-patella syndrome, in which the glomerular changes are present in the absence of the usual associated constitutional abnormalities.
Asunto(s)
Glomérulos Renales/ultraestructura , Síndrome de la Uña-Rótula/patología , Adolescente , Membrana Basal/ultraestructura , Colágeno/ultraestructura , Diagnóstico Diferencial , Técnica del Anticuerpo Fluorescente , Humanos , Masculino , Microscopía Electrónica , Síndrome de la Uña-Rótula/complicacionesRESUMEN
A young woman with a melanoma of the left forearm was found to have a right lung mass. This was initially interpreted as metastatic melanoma on the basis of clinical, radiographic, and light microscopic features, together with positive staining of tumor cells with antibody HMB-45. Electron microscopic examination performed for confirmation of the diagnosis revealed no evidence of melanocytic differentiation. Instead, there were features suggestive of the alternative diagnosis of sclerosing hemangioma (SH). This diagnosis was confirmed with additional immunocytochemical stains. To the authors' knowledge this is the first report of HMB-45 positivity in SH. This case illustrates a potentially disastrous diagnostic pitfall in interpreting lung tumors in patients with melanoma, and the vital role of electron microscopy in resolving conflicting and/or misleading immunocytochemical results.