Quantum simulator to emulate lower-dimensional molecular structure.

Bottom-up quantum simulators have been developed to quantify the role of various interactions, dimensionality, and structure in creating electronic states of matter. Here, we demonstrated a solid-state quantum simulator emulating molecular orbitals, based solely on positioning individual cesium atoms on an indium antimonide surface. Using scanning tunneling microscopy and spectroscopy, combined with ab initio calculations, we showed that artificial atoms could be made from localized states created from patterned cesium rings. These artificial atoms served as building blocks to realize artificial molecular structures with different orbital symmetries. These corresponding molecular orbitals allowed us to simulate two-dimensional structures reminiscent of well-known organic molecules. This platform could further be used to monitor the interplay between atomic structures and the resulting molecular orbital landscape with submolecular precision.

Intestinal and hepatic contributions to the pharmacokinetic interaction between gamithromycin and rifampicin after single-dose and multiple-dose administration in healthy foals.

BACKGROUND: Standard treatment of foals with severe abscessing lung infection caused by Rhodococcus equi using rifampicin and a macrolide antibiotic can be compromised by extensive inhibition and/or induction of drug metabolising enzymes (e.g. CYP3A4) and transport proteins (e.g. P-glycoprotein), as has been shown for rifampicin and clarithromycin. The combination of rifampicin with the new, poorly metabolised gamithromycin, a long-acting analogue of azithromycin and tulathromycin with lower pharmacokinetic interaction potential, might be a suitable alternative. OBJECTIVES: To evaluate the pharmacokinetic interactions and pulmonary distribution of rifampicin and gamithromycin in healthy foals, and to investigate the cellular uptake of gamithromycin in vitro. STUDY DESIGN: Controlled, four-period, consecutive, single-dose and multiple-dose study. METHODS: Pharmacokinetics and lung distribution of rifampicin (10 mg/kg) and gamithromycin (6 mg/kg) were measured in nine healthy foals using LC-MS/MS. Enzyme induction was confirmed using the 4ß-OH-cholesterol/cholesterol ratio. Affinity of gamithromycin to drug transport proteins was evaluated in vitro using equine hepatocytes and MDCKII-cells stably transfected with human OATP1B1, OATP1B3 and OATP2B1. RESULTS: Rifampicin significantly (P<0.05) increased the plasma exposure of gamithromycin (16.2 ± 4.77 vs. 8.57 ± 3.10 µg × h/mL) by decreasing the total body clearance. Otherwise, gamithromycin significantly lowered plasma exposure of single- and multiple-dose rifampicin (83.8 ± 35.3 and 112 ± 43.1 vs. 164 ± 96.7 µg × h/mL) without a change in metabolic ratio and half-life. Gamithromycin was identified as an inhibitor of human OATP1B1, OATP1B3 and OATP2B1 and as a substrate of OATP2B1. In addition, it was extracted by equine hepatocytes via a mechanism which could be inhibited by rifampicin. MAIN LIMITATIONS: Influence of gamithromycin on pulmonary distribution of rifampicin was not evaluated. CONCLUSION: The plasma exposure of gamithromycin is significantly increased by co-administration of rifampicin which is most likely caused by inhibition of hepatic elimination.

[Urolithiasis in the Long-Term GnRH Agonist Treatment of Patients with Paraphilia: 3 Case Studies]. / Urolithiasis bei der Langzeitherapie mit GnRH-Agonisten bei Paraphilien ­ 3 Fallberichte.

The outpatient forensic aftercare department of the Charité Berlin treated 32 paraphilic sex offenders with GnRH analogues within the past 5 years. Out of those patients, three men suffered from urolithiasis and were in need of treatment. All 3 patients had previously developed osteopenia/osteoporosis while on antiandrogen treatment.This article describes these 3 cases and suggests an intense consideration of the possible occurrence of urolithiasis in sex offenders on antiandrogen treatment.

Respiratory failure in a term infant with cis and trans mutations in ABCA3.

A full-term female neonate presented with persistent respiratory failure and radiologic studies consistent with surfactant deficiency. Sequencing of the ATP-binding cassette transporter A3 gene (ABCA3) revealed three mutations: R280C, V1399M and Q1589X. The infant underwent bilateral lung transplantation at 9 months of age and is alive at 3 years of age. Parental sequencing demonstrated that two of the mutations (R280C and Q1589X) were oriented on the same allele (cis), whereas V1399M was oriented on the opposite allele (trans). As more than one mutation in ABCA3 can be present on the same allele, parental studies are needed to determine allelic orientation to inform clinical decision making and future reproductive counseling.

Electronic structure and dynamics of quantum-well states in thin Yb metal films.

Quantum-well states above the Fermi energy in thin Yb(111) metal films deposited on a W(110) single crystal were studied by low-temperature scanning tunneling spectroscopy. These states are laterally highly localized and give rise to sharp peaks in the tunneling spectra. A quantitative analysis of the spectra yields the bulk-band dispersion in the Gamma-L direction as well as quasiparticle lifetimes. The quadratic energy dependence of the lifetimes is in quantitative agreement with Fermi-liquid theory.

Magneto-optical near-field microscopy of ultrathin films in ultrahigh vacuum.

We demonstrate that magnetic domains of ultrathin films can be imaged in situ in ultrahigh vacuum (UHV) by scanning near-field optical microscopy in combination with a Sagnac interferometer (Sagnac-SNOM). In the present set-up, the Sagnac interferometer is not only used for measuring the magneto-optical Kerr effect but also for tip-to-sample distance control. The high sensitivity of the instrument enabled us to study the magnetization reversal processes in externally applied magnetic fields in ultrathin films of Fe and Ni grown on Cu(100) single crystals.

A simple technique for testing the viability of cross-finger pedicle flaps.

A simple test to determine the viability of cross-finger pedicle flaps is described. This test can be performed in the office or operating theatre to help determine the optimal timing for division of the flap pedicle.

Prevalence of anemia in elderly subjects living at home: role of micronutrient deficiency and inflammation.

OBJECTIVE: Aging is associated with increased risk of developing anemia and micronutrient deficiencies. Wheat-based staple foods are iron fortified in Chile. We aimed to establish the prevalence and etiology of anemia in apparently healthy free-living elderly subjects. DESIGN AND SETTING: A cross-sectional study was performed in an outpatient clinic of Santiago, Chile. SUBJECTS AND METHODS: A group of 274 subjects (93 men, 181 women)>/=60 y old living at home and apparently healthy was studied. Clinical and anthropometric evaluations and dietary survey were performed. Complete blood count, and status of iron, copper, folate, vitamins B12 and A and C-reactive protein, and erythrocyte sedimentation rate were measured. RESULTS: Prevalence of anemia was 5.4% for men and 4.4% for women. Subjects with inflammatory process had a higher prevalence of anemia (22.2% men, 31.6% women). Abnormal serum retinol (<0.35 micromol/l) was found in 13.7% of men and 15.9% of women. Prevalence of folate deficiency (<7 nmol/l) was 50.5% in men and 33.1% in women. Low serum vitamin B12 (<148 pmol/l) was present in 51.1% of men and 30. 9% of women. Iron and copper deficiencies were infrequent. CONCLUSIONS: Anemia is not prevalent in free-living elderly subjects when iron intake is adequate. Inflammatory process is the main etiology of anemia in this age group. Vitamin A, folate and vitamin B12 deficiencies were found in a significant proportion of the study group. SPONSORSHIP: Sandoz Foundation for Gerontological Research.

Thought suppression.

Although thought suppression is a popular form of mental control, research has indicated that it can be counterproductive, helping assure the very state of mind one had hoped to avoid. This chapter reviews the research on suppression, which spans a wide range of domains, including emotions, memory, interpersonal processes, psychophysiological reactions, and psychopathology. The chapter considers the relevant methodological and theoretical issues and suggests directions for future research.

Covering up what can't be seen: concealable stigma and mental control.

In these studies the authors examined the effects of concealing a stigma in a social interaction relevant to the stigma. An interview paradigm called for undergraduate female participants who either did or did not have eating disordered characteristics to play the role of someone who did or did not have an eating disorder (ED) while answering stigma-relevant questions. The data suggest that the participants who concealed their stigmas become preoccupied with the control of stigma-relevant thoughts. In Study 1, participants with an ED who role-played not having an ED exhibited more secrecy, suppression, and intrusive thoughts of their ED and more projection of ED-related thoughts onto the interviewer than did those with an ED who role-played someone with an ED or those without an ED who role-played someone without an ED. This finding was replicated in Study 2, and the authors found both increasing accessibility of ED-related words among those participants with concealed stigmas during the interview and high levels of accessibility following the interview.

Apparent mental causation. Sources of the experience of will.

The experience of willing an act arises from interpreting one's thought as the cause of the act. Conscious will is thus experienced as a function of the priority, consistency, and exclusivity of the thought about the action. The thought must occur before the action, be consistent with the action, and not be accompanied by other causes. An experiment illustrating the role of priority found that people can arrive at the mistaken belief that they have intentionally caused an action that in fact they were forced to perform when they are simply led to think about the action just before its occurrence.

Magnetic domain imaging with a scanning near-field optical microscope using a modified Sagnac interferometer.

We report on the combination of a scanning near-field optical microscope and a modified Sagnac interferometer for magnetic-domain imaging in the reflection mode. The Sagnac interferometer is used for detection of the magneto-optical Kerr effect. Since the interferometer is inherently insensitive to polarization changes caused by topography effects, magnetic-domain imaging is not limited to samples with flat surfaces. In this way, it is possible to image magnetic bits written on the tracks of a magneto-optical disc that has a rather pronounced surface profile.

Effect of proximal phalangeal fracture deformity on extensor tendon function.

Extensor lag is a common sequella of proximal phalangeal fractures. Proximal phalangeal fractures frequently lead to extensor tendon dysfunction via adhesions and phalanx malunion with angulation and/or shortening. The primary purpose of this study was to quantify the influence of proximal phalangeal angulation and shortening on proximal interphalangeal (PIP) joint extension by examining the effect of isolated proximal phalangeal bone to extensor digitorum communis tendon discrepancy on PIP joint lag. The secondary goal was to determine the reserve of the extensor digitorum communis to overcome the PIP joint lag. A proximal phalangeal bone-extensor tendon discrepancy results in either a PIP lag or a potential lag that must be compensated for by another system, such as by overpull of the extrinsic extensor or by the pull of the intrinsics. Six cadaveric middle fingers were used, proximal phalangeal malunions were simulated, and the extensor lag was measured radiographically. A linear relationship between extensor tendon lengthening and the resulting PIP lag was observed. Similarly, a linear relationship between proximal phalangeal shortening and the lag was observed. For both, the average slope was 12 degrees of lag/mm of bone-tendon discrepancy. For average apex palmar angulations of 16 degrees, 27 degrees, and 46 degrees, PIP lags of 10 degrees, 24 degrees, and 66 degrees, respectively, resulted. The extensor tendon reserve was 2 to 6 mm. The sagittal bands became tight at the limits of the extensor tendon reserve. The results of this study quantify the importance of re-establishing the bone-tendon relationship for proximal phalangeal fractures.

Baculovirus surface display: construction and screening of a eukaryotic epitope library.

The baculovirus expression system was utilized to serve as a tool for ligand selection, demonstrating the applicability of the system to the generation and screening of eukaryotic expression libraries. The HIV-1-gp41 epitope 'ELDKWA', specific for the neutralizing human mAb 2F5, was inserted into the antigenic site B of influenza virus hemagglutinin and expressed on the surface of baculovirus infected insect cells. In order to improve the antigenicity of the epitope within the hemagglutinin, and therefore enhance the specific binding of 2F5, we inserted three additional, random amino acids adjacent to the epitope. This pool of hemagglutinin genes was directly cloned into the baculovirus Ac-omega. To identify distinct proteins displayed on the cellular surface, we developed a screening protocol to select for specific binding capacity of individual viral clones. Using fluorescence activated cell sorting (FACS) we isolated a baculovirus clone displaying the epitope with markedly increased binding capacity out of a pool of 8000 variants in only one sorting step. Binding properties of the identified ligand were examined by FACS performing a competition assay.

Ironic effects of trying to relax under stress.

Two studies found that intentional relaxation under conditions of mental load or stress produces ironic increases in skin conductance level (SCL). In Experiment 1, participants instructed to relax under the high mental load of rehearsing a long number had higher SCL than those instructed to relax under low load, and tended to have higher SCL than those under high load not instructed to relax. In Experiment 2, participants were instructed to relax or were not so instructed while they answered questions described either as measures of IQ or as unimportant. Those in the more loading and stressful situation who were asked to relax had greater SCL during the questions than those not asked to relax.

Deep cognitive activation: a new approach to the unconscious.

Deep cognitive activation occurs when a thought is so accessible as to have measurable effects on behavior or judgement, but is yet not consciously reportable. This state of mind has unique properties mimicking some characteristics of the psychoanalytic unconscious, but following theoretically from a consideration of processes of cognitive activation. The sources and consequences of deep cognitive activation are examined, with a view toward understanding how this state is implicated in the assessment, etiology, and treatment of psychopathology.

Memories out of order: thought suppression and the disturbance of sequence memory.

Participants in 2 experiments watched a filmed story and then left the lab--with instructions not to think about the film, with instructions to think about the film, or with no instructions. Memories of the film, assessed on participants' return to the lab some 5 hr later, showed reliable effects of thought suppression on memory for the sequence of events in the film. Participants who suppressed thoughts of the film were less able to retrieve the order of events by several measures than were those in the other groups, even thought their retrieval of the events themselves as assessed by recognition, free recall, and cued recall was not generally impaired.

Ironic effects of sleep urgency.

Normal sleepers were instructed either to fall asleep as quickly as they could or to fall asleep whenever they desired, under a high mental load (listening to John Philip Sousa marches) or a low mental load (listening to sleep-conducive new age music). Under low load, participants trying to fall asleep quickly did so faster than those attempting only to fall asleep whenever they desired. Under high load, however, and consistent with the ironic process theory of mental control (Wegner, D. M., 1994, Psychological Review, 101, 34-52), sleep onset latency was greater for participants attempting to fall asleep quickly than for those not attempting to do so.