A Novel Numerical Model of Gelant Inaccessible Pore Volume for In Situ Gel Treatment.

Inaccessible pore volume (IAPV) can have an important impact on the placement of gelant during in situ gel treatment for conformance control. Previously, IAPV was considered to be a constant factor in simulators, yet it lacked dynamic characterization. This paper proposes a numerical simulation model of IAPV. The model was derived based on the theoretical hydrodynamic model of gelant molecules. The model considers both static features, such as gelant and formation properties, and dynamic features, such as gelant rheology and retention. To validate our model, we collected IAPV from 64 experiments and the results showed that our model fit moderately into these lab results, which proved the robustness of our model. The results of the sensitivity test showed that, considering rheology and retention, IAPV in the matrix dramatically increased when flow velocity and gelant concentration increased, but IAPV in the fracture maintained a low value. Finally, the results of the penetration degree showed that the high IAPV in the matrix greatly benefited gelant placement near the wellbore situation with a high flow velocity and gelant concentration. By considering dynamic features, this new numerical model can be applied in future integral reservoir simulators to better predict the gelant placement of in situ gel treatment for conformance control.

Pattern Recognition for Steam Flooding Field Applications Based on Hierarchical Clustering and Principal Component Analysis.

Steam flooding is a complex process that has been considered as an effective enhanced oil recovery technique in both heavy oil and light oil reservoirs. Many studies have been conducted on different sets of steam flooding projects using the conventional data analysis methods, while the implementation of machine learning algorithms to find the hidden patterns is rarely found. In this study, a hierarchical clustering algorithm (HCA) coupled with principal component analysis is used to analyze the steam flooding projects worldwide. The goal of this research is to group similar steam flooding projects into the same cluster so that valuable operational design experiences and production performance from the analogue cases can be referenced for decision-making. Besides, hidden patterns embedded in steam flooding applications can be revealed based on data characteristics of each cluster for different reservoir/fluid conditions. In this research, principal component analysis is applied to project original data to a new feature space, which finds two principal components to represent the eight reservoir/fluid parameters (8D) but still retain about 90% of the variance. HCA is implemented with the optimized design of five clusters, Euclidean distance, and Ward's linkage method. The results of the hierarchical clustering depict that each cluster detects a unique range of each property, and the analogue cases present that fields under similar reservoir/fluid conditions could share similar operational design and production performance.

Direct Pore-Level Visualization and Verification of In Situ Oil-in-Water Pickering Emulsification during Polymeric Nanogel Flooding for EOR in a Transparent Three-Dimensional Micromodel.

Different from inorganic nanoparticles, nanosized cross-linked polymeric nanoparticles (nanogels) have been demonstrated to generate more stable Pickering emulsions under harsh conditions for a long term owing to their inherent high hydrophilicity and surface energy. In both core and pore scales, the emulsions are found to be able to form in situ during the nanofluid flooding process for an enhanced oil recovery (EOR) process. Due to the limitation of direct visualization in core scale or deficient pore geometries built by two-dimensional micromodels, the in situ emulsification by nanofluids and emulsion transport are still not being well understood. In this work, we use a three-dimensional transparent porous medium to directly visualize the in situ emulsification during the nanogel flooding process for EOR after water flooding. By synthesizing the nanogel with a fluorescent dye, we find the nanogels adsorbed on the oil-water interface to lower the total interfacial energy and emulsify the large oil droplets into small Pickering oil-in-water emulsions. A potential mechanism for in situ emulsification by nanogels is proposed and discussed. After nanogel flooding, the emulsions trapped in pore throats and those in the effluents are all found encapsulated by the nanogels. After nanogel flooding under different flow rates, the sphericity and diameter changes of remaining oil droplets are quantitatively compared and analyzed using grouped boxplots. It is concluded that in situ emulsification happens during nanogel injection due to the reduction of interfacial tension, which helps to increase the oil recovery rate under different flow rates and pore geometries.

A review of wettability alteration using surfactants in carbonate reservoirs.

The wettability of carbonate rocks is often oil-wet or mixed wet. A large fraction of oil is still remained in carbonate reservoirs, it is therefore of particular significance to implement effective methods to improve oil recovery from carbonate reservoirs. Altering wettability from oil-wet to more favorable water-wet has been proven successful to achieve this goal. Surfactants are widely investigated and served as wettability modifiers in this process. Yet a comprehensive review of altering wettability of carbonate reservoirs with surfactants is ignored in literature. This study begins with illustration of wettability evolution process in carbonate reservoirs. Techniques to evaluate wettability alteration extent or to reveal behind mechanisms are also presented. Several surfactant systems are analyzed in terms of their wettability alteration mechanisms, influential factors of performance, applicable conditions, and limitations. Mixture of different types of surfactants could obtain synergic effects, where applicable conditions are extended, and final performance is improved. Gemini surfactants have many desirable properties, which warrants further investigation for understanding their wettability alteration mechanisms and performance. At the end, this review discusses strategies related with surfactant cost, surfactant adsorption, and challenges at high temperature, high salinity reservoirs conditions. Also, some unclear issues are discussed.

Fabrication and verification of a glass-silicon-glass micro-/nanofluidic model for investigating multi-phase flow in shale-like unconventional dual-porosity tight porous media.

Unconventional shale or tight oil/gas reservoirs that have micro-/nano-sized dual-scale matrix pore throats with micro-fractures may result in different fluid flow mechanisms compared with conventional oil/gas reservoirs. Microfluidic models, as a potential powerful tool, have been used for decades for investigating fluid flow at the pore-scale in the energy field. However, almost all microfluidic models were fabricated by using etching methods and very few had dual-scale micro-/nanofluidic channels. Herein, we developed a lab-based, quick-processing and cost-effective fabrication method using a lift-off process combined with the anodic bonding method, which avoids the use of any etching methods. A dual-porosity matrix/micro-fracture pattern, which can mimic the topology of shale with random irregular grain shapes, was designed with the Voronoi algorithm. The pore channel width range is 3 µm to 10 µm for matrices and 100-200 µm for micro-fractures. Silicon is used as the material evaporated and deposited onto a glass wafer and then bonded with another glass wafer. The channel depth is the same (250 nm) as the deposited silicon thickness. By using an advanced confocal laser scanning microscopy (CLSM) system, we directly visualized the pore level flow within micro/nano dual-scale channels with fluorescent-dyed water and oil phases. We found a serious fingering phenomenon when water displaced oil in the conduits even if water has higher viscosity and the residual oil was distributed as different forms in the matrices, micro-fractures and conduits. We demonstrated that different matrix/micro-fracture/macro-fracture geometries would cause different flow patterns that affect the oil recovery consequently. Taking advantage of such a micro/nano dual-scale 'shale-like' microfluidic model fabricated by a much simpler and lower-cost method, studies on complex fluid flow behavior within shale or other tight heterogeneous porous media would be significantly beneficial.

DRAM1 deficiency affects the organization and function of the Golgi apparatus.

DRAM1 (DNA damage-regulated autophagy modulator 1) is a transmembrane protein that predominantly localizes to the lysosome but is also found in other membranous organelles; however, its function in these organelles remains largely unknown. We found that DRAM1 was partially located in the Golgi apparatus, and knockdown of DRAM1 caused fragmentation of the Golgi apparatus in cells. The phenomenon of fragmented Golgi was not related to microtubule organization, and there was no direct interaction between DRAM1 and Golgi structural proteins (ARF1, GM130, syntaxin 6 and GRASP55). Moreover, Golgi-targeting DRAM1 failed to rescue the fragmentation of Golgi in DRAM1-deficient cells. The transport of ts045-VSVG-GFP, an indicator of movement from the Golgi apparatus to the plasma membrane, was delayed in DRAM1-knockdown cells. Moreover, the trafficking of CI-MPR from the plasma membrane to the Golgi was also impeded in DRAM1-knockdown cells. These results indicated that DRAM1 regulated the structure of the Golgi apparatus and affected Golgi apparatus-associated vesicular transport.

TIGAR regulates mitochondrial functions through SIRT1-PGC1α pathway and translocation of TIGAR into mitochondria in skeletal muscle.

TP53-induced glycolysis and apoptosis regulator (TIGAR), a glycolytic inhibitor, plays vital roles in regulating cellular metabolism and oxidative stress. However, the role of highly expressed TIGAR in skeletal muscle remains unexplored. In the present study, TIGAR levels varied in different skeletal muscles and fibers. An exhaustive swimming test with a load corresponding to 5% of body weight was utilized in mice to assess the effects of TIGAR on exercise-induced fatigue and muscle damage. The running time and metabolic indicators were significantly greater in wild-type (WT) mice compared with TIGAR knockout (KO) mice. Poor exercise capacity was accompanied by decreased type IIA fibers in TIGAR KO mice. Decreased mitochondrial number and mitochondrial oxidative phosphorylation were observed more in TIGAR KO mice than in WT mice, which were involved in sirtuin 1 (SIRT1)-mediated deacetylation of peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α), and resveratrol treatment in TIGAR KO mice can increase mitochondrial content and exercise time. Much more TIGAR was also detected in mitochondria during exhaustive exercise. In addition, TIGAR, rather than mitochondria-targeted TIGAR achieved by in vitro plasmid transfection, promoted SIRT1-PGC1α pathway. Glutathione S-transferase-TIGAR pull-down assay followed by liquid chromatography mass spectrometry found that TIGAR interacted with ATP synthase F1 subunit α (ATP5A1), and its binding to ATP5A1 increased during exhaustive exercise. Overexpression of mitochondrial-TIGAR enhanced ATP generation, maintained mitochondrial membrane potential and reduced mitochondrial oxidative stress under hypoxia condition. Taken together, our results uncovered a novel role for TIGAR in mitochondrial regulation in fast-twitch oxidative skeletal muscle through SIRT1-PGC1α and translocation into mitochondria, which contribute to the increase in exercise endurance of mice.-Geng, J., Wei, M., Yuan, X., Liu, Z., Wang, X., Zhang, D., Luo, L., Wu, J., Guo, W., Qin, Z.-H. TIGAR regulates mitochondrial functions through SIRT1-PGC1α pathway and translocation of TIGAR into mitochondria in skeletal muscle.

The diverse role of TIGAR in cellular homeostasis and cancer.

TP53-induced glycolysis and apoptosis regulator (TIGAR) is a p53 target protein that plays critical roles in glycolysis and redox balance. Accumulating evidence shows that TIGAR is highly expressed in cancer. TIGAR redirects glycolysis and promotes carcinoma growth by providing metabolic intermediates and reductive power derived from pentose phosphate pathway (PPP). The expression of TIGAR in cancer is positively associated with chemotherapy resistance, suggesting that TIGAR could be a novel therapeutic target. In this review, we briefly presented the function of TIGAR in metabolic homeostasis in normal and cancer cells. Finally, we discussed the future directions of TIGAR research in cancer.

Study of gas production from shale reservoirs with multi-stage hydraulic fracturing horizontal well considering multiple transport mechanisms.

Development of unconventional shale gas reservoirs (SGRs) has been boosted by the advancements in two key technologies: horizontal drilling and multi-stage hydraulic fracturing. A large number of multi-stage fractured horizontal wells (MsFHW) have been drilled to enhance reservoir production performance. Gas flow in SGRs is a multi-mechanism process, including: desorption, diffusion, and non-Darcy flow. The productivity of the SGRs with MsFHW is influenced by both reservoir conditions and hydraulic fracture properties. However, rare simulation work has been conducted for multi-stage hydraulic fractured SGRs. Most of them use well testing methods, which have too many unrealistic simplifications and assumptions. Also, no systematical work has been conducted considering all reasonable transport mechanisms. And there are very few works on sensitivity studies of uncertain parameters using real parameter ranges. Hence, a detailed and systematic study of reservoir simulation with MsFHW is still necessary. In this paper, a dual porosity model was constructed to estimate the effect of parameters on shale gas production with MsFHW. The simulation model was verified with the available field data from the Barnett Shale. The following mechanisms have been considered in this model: viscous flow, slip flow, Knudsen diffusion, and gas desorption. Langmuir isotherm was used to simulate the gas desorption process. Sensitivity analysis on SGRs' production performance with MsFHW has been conducted. Parameters influencing shale gas production were classified into two categories: reservoir parameters including matrix permeability, matrix porosity; and hydraulic fracture parameters including hydraulic fracture spacing, and fracture half-length. Typical ranges of matrix parameters have been reviewed. Sensitivity analysis have been conducted to analyze the effect of the above factors on the production performance of SGRs. Through comparison, it can be found that hydraulic fracture parameters are more sensitive compared with reservoir parameters. And reservoirs parameters mainly affect the later production period. However, the hydraulic fracture parameters have a significant effect on gas production from the early period. The results of this study can be used to improve the efficiency of history matching process. Also, it can contribute to the design and optimization of hydraulic fracture treatment design in unconventional SGRs.

Modeling of Gas Production from Shale Reservoirs Considering Multiple Transport Mechanisms.

Gas transport in unconventional shale strata is a multi-mechanism-coupling process that is different from the process observed in conventional reservoirs. In micro fractures which are inborn or induced by hydraulic stimulation, viscous flow dominates. And gas surface diffusion and gas desorption should be further considered in organic nano pores. Also, the Klinkenberg effect should be considered when dealing with the gas transport problem. In addition, following two factors can play significant roles under certain circumstances but have not received enough attention in previous models. During pressure depletion, gas viscosity will change with Knudsen number; and pore radius will increase when the adsorption gas desorbs from the pore wall. In this paper, a comprehensive mathematical model that incorporates all known mechanisms for simulating gas flow in shale strata is presented. The objective of this study was to provide a more accurate reservoir model for simulation based on the flow mechanisms in the pore scale and formation geometry. Complex mechanisms, including viscous flow, Knudsen diffusion, slip flow, and desorption, are optionally integrated into different continua in the model. Sensitivity analysis was conducted to evaluate the effect of different mechanisms on the gas production. The results showed that adsorption and gas viscosity change will have a great impact on gas production. Ignoring one of following scenarios, such as adsorption, gas permeability change, gas viscosity change, or pore radius change, will underestimate gas production.

Study of displacement efficiency and flow behavior of foamed gel in non-homogeneous porous media.

Field trials have demonstrated that foamed gel is a very cost-effective technology for profile modification and water shut-off. However, the mechanisms of profile modification and flow behavior of foamed gel in non-homogeneous porous media are not yet well understood. In order to investigate these mechanisms and the interactions between foamed gel and oil in porous media, coreflooding and pore-scale visualization waterflooding experiments were performed in the laboratory. The results of the coreflooding experiment in non-homogeneous porous media showed that the displacement efficiency improved by approximately 30% after injecting a 0.3 pore volume of foamed gel, and was proportional to the pore volumes of the injected foamed gel. Additionally, the mid-high permeability zone can be selectively plugged by foamed gel, and then oil located in the low permeability zone will be displaced. The visualization images demonstrated that the amoeba effect and Jamin effect are the main mechanisms for enhancing oil recovery by foamed gel. Compared with conventional gel, a unique benefit of foamed gel is that it can pass through micropores by transforming into arbitrary shapes without rupturing, this phenomenon has been named the amoeba effect. Additionally, the stability of foam in the presence of crude oil also was investigated. Image and statistical analysis showed that these foams boast excellent oil resistance and elasticity, which allows them to work deep within formations.