Analysing substitutions in recent World Cups and European Championships in male and female elite football - influence of new substitution rules.

Substitutions play a key role in modern football and can substantially affect the physical and overall performance of a team, and the recent substitution rule changes are worth investigating. This study explored the characteristics of substitutions, including different substitution rules, game results, sex, competition stages, tournaments and penalty shoot-outs success rates. We analysed data from a total of 3,738 substitutions from the last 10 years (2013-2023) of European Championships and World Cups, both men's and women's games. Non-parametric tests and chi-square tests were used for statistical analysis with the significance level set at p < 0.05. With the 5-substitution rule, 48% more substitutions occurred compared to the 3-substitution rule (4.26 ± 1.07 vs. 2.87 ± 0.43, p < 0.05) with a slight increase in the average substitution time (70.6 ± 14.3 vs. 69.2 ± 14.6 min, p < 0.05), and 10% more substitutions in the men's game compared to the women's game (p < 0.05). The timing of the first substitution was slightly different in the knock-out stage compared to group stage (59.8 ± 14.7 vs. 57.2 ± 13.3 min, p < 0.05), and the timing for the winning team and drawing team was later than for the losing team (p < 0.05). A total of 13.2% goals were scored by substitutes, with no significant difference between the 5-substitution rule (15.9%) vs the 3-substition rule (12.5%) (p > 0.05). Interestingly, substitute players had a lower success rate in penalty shoot-out compared to starters (61 vs. 74%, p < 0.05). Additionally, substitute player goal scorers entered the pitch later (p < 0.05) in male games compared to female games and in knock-out stage games compared to group games. This study highlights the importance of substitution rules and timing in modern elite football matches. The timing of the first substitution, introduction of substitutes in knock-out stages, and a lower success rate of substitute players in penalty shoot-outs are crucial factors to consider. Coaches can use this information to make strategic substitution decisions to improve team performance.

To characterize small renal cell carcinoma using diffusion relaxation correlation spectroscopic imaging and apparent diffusion coefficient based histogram analysis: a preliminary study.

PURPOSE: To study the capability of diffusion-relaxation correlation spectroscopic imaging (DR-CSI) on subtype classification and grade differentiation for small renal cell carcinoma (RCC). Histogram analysis for apparent diffusion coefficient (ADC) was studied for comparison. MATERIALS AND METHODS: A total of 61 patients with small RCC (< 4 cm) were included in the retrospective study. MRI data were reviewed, including a multi-b (0-1500 s/mm2) multi-TE (51-200 ms) diffusion weighted imaging (DWI) sequence. Region of interest (ROI) was delineated manually on DWI to include solid tumor. For each patient, a D-T2 spectrum was fitted and segmented into 5 compartments, and the volume fractions VA, VB, VC, VD, VE were obtained. ADC mapping was calculated, and histogram parameters ADC 90th, 10th, median, standard deviation, skewness and kurtosis were obtained. All MRI metrices were compared between clear cell RCC (ccRCC) and non-ccRCC group, and between high-grade and low-grade group. Receiver operator curve analysis was used to assess the corresponding diagnostic performance. RESULTS: Significantly higher ADC 90th, ADC 10th and ADC median, and significantly lower DR-CSI VB was found for ccRCC compared to non-ccRCC. Significantly lower ADC 90th, ADC median and significantly higher VB was found for high-grade RCC compared to low-grade. For identifying ccRCC from non-ccRCC, VB showed the highest area under curve (AUC, 0.861) and specificity (0.882). For differentiating high- from low-grade, ADC 90th showed the highest AUC (0.726) and specificity (0.786), while VB also displayed a moderate AUC (0.715). CONCLUSION: DR-CSI may offer improved accuracy in subtype identification for small RCC, while do not show better performance for small RCC grading compared to ADC histogram.

Relationship between team ranking and physical fitness in elite male handball players in different playing positions.

The aim of this study was to identify the key physical indicators that affect game performance of male team handball athletes in national team handball tournaments and explore them further in relation to playing positions. A total of 150 male handball athletes were tested before a national tournament, using squat, bench press, 30-m sprint, vertical jump, pull-ups and abdominal endurance testing. Correlation analysis was used to examine the potential relationship between fitness level and tournament ranking. The results revealed significant differences in fitness variables among different playing positions (p < 0.05), excepted abdominal endurance and vertical jump (p > 0.05). 1RM squad performance was associated with team rankings (r = 0.289, p < 0.05). For individual playing positions, bench press of backs correlate with rankings (r = 0.354, p < 0.05). For goalkeepers, ranking was significantly positively correlated with 30-m sprinting (r = 0.604, p < 0.05). No other correlations were found (p > 0.05). To conclude, fitness level is to some extent related to team ranking with higher ranked teams having better scores in some of the fitness tests for all playing positions. The key predictors of performance in sport vary from position to position and practitioners need to differentiate between them to organize strength and conditioning training more precisely. More specialized fitness tests are also needed to assess athletes' competition ability.

Investigation of clear cell renal cell carcinoma grades using diffusion-relaxation correlation spectroscopic imaging with optimized spatial-spectrum analysis.

OBJECTIVES: To differentiate high-grade from low-grade clear cell renal cell carcinoma (ccRCC) using diffusion-relaxation correlation spectroscopic imaging (DR-CSI) spectra in an equal separating analysis. METHODS: Eighty patients with 86 pathologically confirmed ccRCCs who underwent DR-CSI were enrolled. Two radiologists delineated the region of interest. The spectrum was derived based on DR-CSI and was further segmented into multiple equal subregions from 2*2 to 9*9. The agreement between the 2 radiologists was assessed by the intraclass correlation coefficient (ICC). Logistic regression was used to establish the regression model for differentiation, and 5-fold cross-validation was used to evaluate its accuracy. McNemar's test was used to compare the diagnostic performance between equipartition models and the traditional parameters, including the apparent diffusion coefficient (ADC) and T2 value. RESULTS: The inter-reader agreement decreased as the divisions in the equipartition model increased (overall ICC ranged from 0.859 to 0.920). The accuracy increased from the 2*2 to 9*9 equipartition model (0.68 for 2*2, 0.69 for 3*3 and 4*4, 0.70 for 5*5, 0.71 for 6*6, 0.78 for 7*7, and 0.75 for 8*8 and 9*9). The equipartition models with divisions >7*7 were significantly better than ADC and T2 (vs ADC: P = .002-.008; vs T2: P = .001-.004). CONCLUSIONS: The equipartition method has the potential to analyse the DR-CSI spectrum and discriminate between low-grade and high-grade ccRCC. ADVANCES IN KNOWLEDGE: The evaluation of DR-CSI relies on prior knowledge, and how to assess the spectrum derived from DR-CSI without prior knowledge has not been well studied.

Are EFI data valuable? Evidence from the 2022 FIFA World Cup group stage.

The 2022 World Cup used new Enhanced Football Intelligence (EFI) data, stoppage time calculation methods and substitution rules that were introduced by FIFA. The aim of this study is to explore the effectiveness of EFI in match analysis and to identify the key indicators that influence the match and provide a reference for coaches' tactical design and training. Data were derived from the FIFA website, including EFI data for 48 matches at the group stage of the Qatar World Cup. A total of 46 indicators were used for analysis and the average values of the corresponding indicators for the different competition results were used in the analysis to identify the key index that determines the outcome of the competition. Apart from scoring more goals and having more assists, winning teams had significantly more attempts on target than drawing and losing teams (p < 0.05); Winning teams had significantly more attempts inside the penalty area, completed defensive line breaks and receptions behind the defensive line than losing teams (p < 0.05). There is no difference in possession between matches with different results (p > 0.05). Goals were significantly correlated with completed defensive line breaks and receptions behind the defensive line (r = 0.27-0.30, p < 0.01). Attempts on target was significantly positively correlated with receptions, final third entries and line breaks (r = 0.31-0.67, p < 0.01) and negatively correlated with defensive pressures applied (r = -0.35, p < 0.01). The efficiency of the offense is more important. Teams need to have more receptions, final third entries and line breaks to get more shots on target rather than possession. This study may help coaches to interpret the game from a multi-dimensional perspective and coaches can use EFI to help their teams improve their match performance.

Goal scoring patterns in the FIFA World Cup 2022 group stage: more goals by substitute players, but no effect of more stoppage time.

BACKGROUND: The 2022 World Cup introduced new substitution rules, tournament periods and stoppage time calculation methods. Thus, the aim of this study was to analyze the goal time characteristics during the FIFA World Cup 2022 group stage in comparison with those of the 2018 event, exploring the potential influence on the goal times after the new substitution rules, stoppage time calculation method, and tournament periods. METHODS: A total of 242 goals were used for analysis. The timing, zones and ways of goals scored were used for the analyses by χ2 Test with a significance level set at P<0.05. RESULTS: More goals were scored in the second half than in the first half (64.2% vs. 35.8%; χ2=9.633, P<0.05) and a significant difference in the goals scored in 15 minutes intervals in 2022 World Cup group stage (χ2=16.000, P<0.05). No difference were observed in the goal scoring time in two World Cups in terms of the different intervals, results, areas, and ways (P>0.05), but more open play goals were scored in the 2022 event (χ2=10.200, P<0.05) and substitutes scored more goals in the 2022 World Cup group stage as compared to those in the 2018 event (χ2=5.161, P<0.05). Despite more stoppage time in the 2022 event than in the 2018 event (11.6 vs. 7.3 min), no differences were observed in the number of goals scored in stoppage time (χ2=0.953, P>0.05). CONCLUSIONS: This study highlights the importance of the insight of goal scoring pattern. Practitioners need to allocate physical fitness to tactical arrangements in a reasonable manner according to the time of the game, improve open play scoring ability and increase substitution awareness.

Development of youth tennis players: A study based on the ranking history of top ATP/WTA players worldwide and China.

BACKGROUND: The top 100 ATP/WTA ranking points are a crucial indicator of entry into the high-level world of tennis players, and the number of players from a nation in this ranking reflects the overall level of their tennis. However, the growth time series characteristics of elite tennis athletes are unclear. OBJECTIVE: This study aims to examine the historical career ranking changes of elite players and provide valuable insights for aspiring young players looking to achieve success in the sport. At the same time, it is of great significance for the efficient and sustainable cultivation of Chinese tennis players. METHODS: Data on the rankings of 202 players were analyzed, Spearman and Pearson correlations were employed to investigate the association between ranking and time-use patterns. The variance test was utilized to compare disparities in time characteristics of the ranking, with a statistical significance level of p<0.05. RESULTS: There was a significant correlation between the time of entering the professional tournament ranking system and the ranking, top 100 time, top 100 age, and age of starting tennis. Top 50 ATP players are earlier than those ranked 51-100. There was a significant difference between the age of starting tennis and the time to top 10 among the ATP and WTA players. Chinese female players showed no significant differences compared to their global Top 10 counterparts in terms of time-to-success characteristics. CONCLUSION: The elite tennis players who achieve success typically start playing and competing in the sport at a young age, with professional competition often commencing around 18 years of age. Notably, these players frequently attain high rankings before reaching the age of 20. Furthermore, top 10 ATP male players tend to start tennis at an earlier age and require a shorter time to achieve a top 10 ranking compared with WTA female players.

Effects of step lengths on biomechanical characteristics of lower extremity during split squat movement.

Objective: To quantify the effects of increasing the step length of the split squat on changes in kinematics, kinetics, and muscle activation of the lower extremity. Methods: Twenty male college students participated in the test (age: 23.9 ± 3.7, height: 175.1 ± 4.9). Data on kinematics, kinetics, and EMG were collected during split squat exercise at four different step lengths in a non-systematic manner. One-way repeated measurements ANOVA were used to compare characteristic variables of peak angle, moment, and RMS among the four step length conditions. Results: The step length significantly changes the peak angles of the hip (p = 0.011), knee (p = 0.001), ankle (p < 0.001) joint, and the peak extension moment of the hip (p < 0.001), knee (p = 0.002) joint, but does not affect the ankle peak extension moment (p = 0.357) during a split squat. Moreover, a significant difference was observed in the EMG of gluteus maximus (p < 0.001), vastus medialis (p = 0.013), vastus lateralis (p = 0.020), biceps femoris (p = 0.003), Semitendinosus (p < 0.001), medialis gastrocnemius (p = 0.035) and lateralis gastrocnemius (p = 0.005) during four step lengths, but no difference in rectus femoris (p = 0.16). Conclusion: Increases in step length of split squat had a greater activation on the hip extensor muscles while having a limited impact on the knee extensor muscles. The ROM, joint moment, and muscle activation of the lead limb in the split squat all should be considered in cases of individual preventative or rehabilitative prescription of the exercise. Moreover, the optimal step length for strength training in healthy adults appears to be more suitable when it is equal to the length of the individual lower extremity.

Association between Serum Cys C and PTB Cavitation.

Background: Cystatin C (Cys C) not only regulates the body's immune defenses but also contributes to tissue degradation and destruction by causing an imbalance between protease and antiprotease in infectious diseases. Is Cys C involved in pulmonary tuberculosis (PTB) infection and cavitation? We therefore conducted a retrospective study on this question to provide a basis for further studies. Methods: Cavitary PTB patients, noncavitary PTB patients, and healthy controls were recruited in our study. Serum Cys C, CRP, BUN, UA, and CR were measured in all subjects, and the Kruskal-Wallis test was used to compare medians of these clinical parameters in different groups. The Spearman rank correlation test was used to determine correlations between variables. In addition, a multivariate analysis using binary logistic regression was used to identify factors associated with PTB cavitation. Results: In our study, elevated serum Cys C levels were found in cavitary PTB patients compared to healthy controls and noncavitary patients (p = 0.022). Serum Cys C levels were statistically correlated with serum BUN and CR concentrations (r = 0.278, p = 0.005; r = 0.281, p = 0.004) in PTB patients. The binary logistic regression analysis showed that elevated serum Cys C levels were correlated with pulmonary cavitation in PTB patients (OR = 1.426, 95% CI: 1.071-1.898). Conclusion: Elevated serum levels of Cys C are associated with pulmonary cavitation in PTB patients.

Detection of prostate cancer using diffusion-relaxation correlation spectrum imaging with support vector machine model - a feasibility study.

BACKGROUND: To evaluate the performance of diffusion-relaxation correlation spectrum imaging (DR-CSI) with support vector machine (SVM) in detecting prostate cancer (PCa). METHODS: In total, 114 patients (mean age, 66 years, range, 48-87 years) who received a prostate MRI and underwent biopsy were enrolled in three stages. Thirty-nine were assigned for the exploration stage to establish the model, 18 for the validation stage to choose the appropriate scale for mapping and 57 for the test stage to compare the diagnostic performance of the DR-CSI and PI-RADS. RESULTS: In the exploration stage, the DR-CSI model was established and performed better than the ADC and T2 values (both P < 0.001). The validation result shows that at least 2 pixels were required for both the long-axis and short-axis in the mapping procedure. In the test stage, DR-CSI had higher accuracy than PI-RADS ≥ 3 as a positive finding based on patient (84.2% vs. 63.2%, P = 0.004) and lesion (78.8% vs. 57.6%, P = 0.001) as well as PI-RADS ≥ 4 on lesion (76.5% vs. 64.7%, P = 0.029), while there was no significant difference between DR-CSI and PI-RADS ≥ 4 based on patient (P = 0.508). For clinically significant PCa, DR-CSI had higher accuracy than PI-RADS ≥ 3 based on patients (84.2% vs. 63.2%, P = 0.004) and lesions (62.4% vs. 48.2%, P = 0.036). There was no significant difference between DR-CSI and PI-RADS ≥ 4 (P = 1.000 and 0.845 for the patient and lesion levels, respectively). CONCLUSIONS: DR-CSI combined with the SVM model may improve the diagnostic accuracy of PCa. TRIAL REGISTRATION: This study was approved by the Ethics Committee of our institute (Approval No. KY2018-213). Written informed consent was obtained from all participants.

Analysis of the association between MICA gene polymorphisms and schizophrenia.

BACKGROUND: The major histocompatibility complex (MHC) has been implicated in schizophrenia. This study aimed to explore the correlation between the major histocompatibility complex class I polypeptide-related sequence A (MICA) polymorphisms and schizophrenia. METHODS: A total of 220 Han schizophrenia patients, 47 Han healthy controls, 155 Li schizophrenia patients, and 48 Li controls were selected from Hainan Province, China. The diagnosis was made according to the Diagnostic and Statistical Manual of Mental Disorders, 4th edition, criteria. Sequencing-based-typing (PCR-SBT) technology was used for MICA allele typing, and the correlation analyses of MICA gene polymorphism and schizophrenia were performed. RESULTS: In the Han group, the three allele frequencies of MICA*002:01, MICA*A4, and MICA*A9 in the schizophrenia group were significantly higher than those in the healthy control group, and the differences were statistically significant (pc < 0.05; pc values were 0.024, 0.030, and 0.031, respectively). Yet, there was no difference in the MICA gene between the schizophrenia group and the healthy controls group in the Li population. CONCLUSION: We found MICA*002:01, MICA*A4, and MICA*A9 may be susceptibility alleles for schizophrenia in the Han population, while the MICA allele polymorphism in the Li population is not associated with schizophrenia in Chinese.

Bacterial Community and Fermentation Quality of Ensiling Alfalfa With Commercial Lactic Acid Bacterial Additives.

The aim of this study was to determine the effects of six common commercial lactic acid bacteria (LAB) additives [A1, Lactobacillus plantarum, L. buchneri, and Enterococcus faecalis; A2, L. plantarum and L. casei; A3, L. plantarum and L. buchneri; A4, L. plantarum, L. buchneri, L. casei, and Pediococcus acidilactici; A5, L. plantarum (producing feruloyl esterase); and A6, L. buchneri, P. acidilactici, ß-glucanase, and xylanase] on the bacterial community and fermentation quality of alfalfa silage. Alfalfa was harvested at the squaring stage, wilted in the field for 24 h, and ensiled without any additives (Control) or with A1, A2, A3, A4, A5, or A6. Microbial counts, bacterial community, fermentation parameters, and nutritional composition were determined after ensiling for 90 days. The total abundance of LAB genera on alfalfa pre-ensiling was 0.38% in bacterial community. The abundances of Lactobacillus, Enterococcus, and Pediococcus in the Control silage were 42.18, 40.18, and 8.09% of abundance, respectively. The abundances of Lactobacillus in A1-, A2-, A3-, A4-, and A5-treatments were 89.32, 92.93, 92.87, 81.12, and 80.44%, respectively. The abundances of Pediococcus and Lactobacillus in A6-treatment were 70.14 and 24.86%, respectively. Compared with Control silage, LAB-treated silage had lower pH and less ammonia nitrogen and water-soluble carbohydrates concentrations (p < 0.05). Further, the A5- and A6-treatments contained lower neutral detergent fiber, acid detergent fiber, and hemicellulose than other treatments (p < 0.05). Overall, LAB genera were presented as minor taxa in alfalfa pre-ensiling and as dominant taxa in alfalfa silage. Adding LAB additives improved the fermentation quality and altered the bacterial community of alfalfa silage. The main bacterial genera in Control silage were Lactobacillus, Enterococcus, and Pediococcus. Lactobacillus dominated the bacterial communities of A1-, A2-, A3-, A4-, and A5-treatments, while Pediococcus and Lactobacillus were dominant bacterial genera in A6-treatment. Inoculating A5 and A6 degraded the fiber in alfalfa silage. It is necessary to ensile alfalfa with LAB inoculants.

A novel HNRNPH1::ERG rearrangement in aggressive acute myeloid leukemia.

FUS::ERG rearrangement is a recurrent abnormality seen in a subgroup of acute myeloid leukemia (AML) with a poor prognosis. We described here a novel HNRNPH1::ERG rearrangement in a de novo AML. The patient was unresponsive to routine chemotherapy and succumbed to the disease just 3 months after diagnosis. Two additional cases of AML with HNRNPH1::ERG rearrangement were discovered by searching a publicly available sequencing database. The three patients share several clinical phenotypes with the FUS::ERG rearranged AML, including high blast count at diagnosis, pediatric or young adult-onset, and poor overall survival. In addition, hnRNPH1 and FUS are both hnRNP family members, a group of RNA-binding proteins functioning in RNA metabolism and transport. Therefore, we suggest that patients with HNRNPH1::ERG or FUS::ERG rearrangement belong to the same distinct clinicopathologic subtype of AML, that is, AML with ERG rearrangement. Based on a previous study showing that FUS::ERG binds to the retinoic acid-responsive elements and that all-trans retinoic acid-induced cell differentiation of AML cells, we support the clinical evaluation of an APL-like therapeutic regimen for AML with ERG rearrangement.

Structural basis of microRNA processing by Dicer-like 1.

MicroRNAs (miRNAs) are short non-coding RNAs that inhibit the expression of target genes by directly binding to their mRNAs. In animals, pri-miRNAs are cleaved by Drosha to generate pre-miRNAs, which are subsequently cleaved by Dicer to generate mature miRNAs. Instead of being cleaved by two different enzymes, both cleavages in plants are performed by Dicer-like 1 (DCL1). With a similar domain architecture as human Dicer, it is mysterious how DCL1 recognizes pri-miRNAs and performs two cleavages sequentially. Here, we report the single-particle cryo-electron microscopy structures of Arabidopsis DCL1 complexed with a pri-miRNA and a pre-miRNA, respectively, in cleavage-competent states. These structures uncover the plasticity of the PAZ domain, which is critical for the recognition of both pri-miRNA and pre-miRNA. These structures suggest that the helicase module serves as an engine that transfers the substrate between two sequential cleavage events. This study lays a foundation for dissecting the regulation mechanism of miRNA biogenesis in plants and provides insights into the dicing state of human Dicer.

Estimation of hydraulic conductivity of saturated frozen soil from the soil freezing characteristic curve.

Knowledge of hydraulic conductivity is crucial for determining water movement in frozen soil, and the objective of this study is to introduce the soil freezing characteristic curve to estimate the hydraulic conductivity of saturated frozen soil. Based on the non-uniform tortuous capillary bundle model and the assumption that the ice was first formed in the pores with largest size, a physical infiltration model of saturated frozen soil was developed. On the basis of this physical infiltration model, a new approach for estimating the hydraulic conductivity of saturated frozen soil was developed by using the Hagen-Poiseuille equation and Darcy's law. To verify the validity of this approach, five soil data sets, including 29 data points with hydraulic conductivity between 10-6 cm/s and 10-11 cm/s, were used to compare the predicted results and experimental data. The results show that the new approach fits the experimental data well. This approach is more convenient than the soil water characteristic curve in numerical modeling, and it can be used to describe the relationship between hydraulic conductivity and minus temperature. Moreover, the new approach and the results in this study maybe also can provide a reference for the research on water flow and the related numerical modeling in cold regions environmental engineering.

Correlation between SEPS1 gene polymorphism and type 2 diabetes mellitus: A preliminary study.

BACKGROUND: The protein encoded by the selenoprotein S gene is considered to be an anti-inflammatory and antioxidant protein and is involved in a variety of diseases. Therefore, we want to study the distribution characteristics of this gene in Chinese diabetic population. METHODS: A total of 170 patients with DM (including 100 patients with T2DM and 70 patients with diabetic nephropathy [DN]) and 100 healthy controls (HC) were selected from Haikou People's Hospital (China) between January 2017 and July 2017. The polymorphisms of three SEPS1 genes (SNP ID: rs4965814, rs28665122, and rs34713741) were measured by massARRAY method, while the polymorphisms of SEPS1 genes (SNP ID: rs4965373) were detected by Sanger sequencing. RESULTS: Comparing three groups, the results were the following: (a) There was a significant difference in the genotype and allele distribution of rs34713741 between DN group and HC group and between T2DM group and DN group; For this gene locus, the risk of diabetic nephropathy in healthy individuals with T allele was 0.6 times higher than that in individuals with GG genotype (OR = 0.60, 95% CI: 0.46 ~ 0.77). (b) There was a significant difference in the distribution of rs4975814 genotype between DN group and HC group; for this gene locus, the risk of diabetic nephropathy in healthy individuals with T allele was 2.71 times higher than that in individuals with GG genotype (OR = 2.71, 95% CI: 1.66 ~ 4.45). CONCLUSION: We conclude that rs34713741 (GT + TT) may be a protective gene for DN and the rs4975814 (GT + TT) may be a susceptibility gene for DN.

P5CR1 protein expression and the effect of gene-silencing on lung adenocarcinoma.

The present study aimed to investigate the expression of pyrroline-5-carboxylate reductase 1 (P5CR1) protein in lung adenocarcinoma and paracancerous tissues and to explore the effect of silencing the encoding gene PYCR1 on the proliferation, migration, invasion, and cisplatin sensitivity in lung adenocarcinoma cells, thereby providing a novel therapeutic target for the treatment of the disease. Immunohistochemistry staining was used to detect the P5CR1 protein expression in lung adenocarcinoma and paracancerous tissues, and statistical analysis evaluated the correlation between P5CR1 protein expression and gender, age, tissue part, or pathological grade. The CCK8 assay was performed to detect the proliferation and cisplatin sensitivity, while the effect of PYCR1 on the migration and invasion of lung adenocarcinoma cells was detected by scratch test and transwell chamber assay. The findings demonstrated that the P5CR1 protein expression was significantly elevated in lung adenocarcinoma tissues and correlated with the pathological grade, whereas no significant correlation was established between the protein expression and gender, age, or tissue part. Furthermore, after PYCR1 gene silencing, the proliferation and invasion were significantly suppressed, while the sensitivity to cisplatin was significantly enhanced. Therefore, it can be speculated that the PYCR1 gene affects the biological behavior of lung adenocarcinoma and cisplatin resistance, serving as a potential therapeutic target for lung adenocarcinoma.

Influence of Water Content on Mechanical Strength and Microstructure of Alkali-Activated Fly Ash/GGBFS Mortars Cured at Cold and Polar Regions.

Negative temperature curing is a very harmful factor for geopolymer mortar or concrete, which will decrease the strength and durability. The water in the geopolymer mixture may be frozen into ice, and the water content is a crucial factor. The purpose of this paper is to explore the influence of water content on the properties of alkali-activated binders mortar cured at -5 °C. Fly ash (FA) and ground granulated blast furnace slag (GGBFS) were used as binders. Three groups of experiments with different water content were carried out. The prepared samples were investigated through uniaxial compression strength test, Scanning electron microscopy (SEM), and X-ray diffraction (XRD) for the determination of their compressive strength, microstructural features, phase, and composition. The results indicated that, the compressive strength of samples basically maintained 25.78 MPa-27.10 MPa at an age of 28 days; for 90 days, the values reached 33.4 MPa-34.04 MPa. The results showed that lower water content is beneficial to improving the early strength of mortar at -5 °C curing condition, while it has little impact on long-term strength. These results may provide references for the design and construction of geopolymer concrete in cold regions.

Structural analysis reveals a "molecular calipers" mechanism for a LATERAL ORGAN BOUNDARIES DOMAIN transcription factor protein from wheat.

LATERAL ORGAN BOUNDARIES DOMAIN (LBD) proteins, a family of plant-specific transcription factors harboring a conserved Lateral Organ Boundaries (LOB) domain, are regulators of plant organ development. Recent studies have unraveled additional pivotal roles of the LBD protein family beyond defining lateral organ boundaries, such as pollen development and nitrogen metabolism. The structural basis for the molecular network of LBD-dependent processes remains to be deciphered. Here, we solved the first structure of the homodimeric LOB domain of Ramosa2 from wheat (TtRa2LD) to 1.9 Å resolution. Our crystal structure reveals structural features shared with other zinc-finger transcriptional factors, as well as some features unique to LBD proteins. Formation of the TtRa2LD homodimer relied on hydrophobic interactions of its coiled-coil motifs. Several specific motifs/domains of the LBD protein were also involved in maintaining its overall conformation. The intricate assembly within and between the monomers determined the precise spatial configuration of the two zinc fingers that recognize palindromic DNA sequences. Biochemical, molecular modeling, and small-angle X-ray scattering experiments indicated that dimerization is important for cooperative DNA binding and discrimination of palindromic DNA through a molecular calipers mechanism. Along with previously published data, this study enables us to establish an atomic-scale mechanistic model for LBD proteins as transcriptional regulators in plants.

The Potential Roles and Advantages of Single Cell Sequencing in the Diagnosis and Treatment of Hematological Malignancies.

Hematological malignancies (HM) are a heterogeneous group of life-threatening hematological diseases. The heterogeneity and clonal evolution of HM subpopulations are the main obstacles for precise diagnoses, risk stratification, and even targeted therapies. Standard bulk-sample genomic examinations average total mutations from multiple subpopulations and conceal the clonal diversity that may play a significant role in HM progression. Therefore, the development of novel methods that detect intra-tumor heterogeneity is critical for the discovery of novel potential therapeutic targets. The recently developed single cell sequencing (SCS) technologies can analyse genetic polymorphisms at a single cell level. SCS requires the precise isolation of single cells and amplification of their genetic material. It allows the analysis of genomic, transcriptomic, and epigenomic information in single cancer cells. SCS may also be able to monitor minimal residual disease (MRD) of HM by sequencing circulating tumor cells (CTCs) from peripheral blood. Functional heterogeneity and clonal evolution exist in acute leukemia, multiple myeloma (MM) and chronic myeloid leukemia (CML) subpopulations and have prognostic value. In this thesis, we provide an overview of SCS technologies in HM and discuss the heterogeneous genetic variation and clonal structure among subpopulations of HM. Furthermore, we aimed to shed light on the clinical applications of SCS technologies, including the development of new targeted therapies for drug-resistant or recurrent HM.