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1.
Clin Genet ; 103(3): 358-363, 2023 03.
Artículo en Inglés | MEDLINE | ID: mdl-36411955

RESUMEN

Aminoacyl-tRNA synthetases are enzymes that ensure accurate protein synthesis. Variants of the dual-functional cytoplasmic human glutamyl-prolyl-tRNA synthetase, EPRS1, have been associated with leukodystrophy, diabetes and bone disease. Here, we report compound heterozygous variants in EPRS1 in a 4-year-old female patient presenting with psychomotor developmental delay, seizures and deafness. Functional studies of these two missense mutations support major defects in enzymatic function in vitro and contributed to confirmation of the diagnosis.


Asunto(s)
Aminoacil-ARNt Sintetasas , Sordera , Epilepsia , Femenino , Humanos , Preescolar , Aminoacilación , Aminoacil-ARNt Sintetasas/genética , Mutación , Epilepsia/diagnóstico , Epilepsia/genética , Convulsiones/genética , Sordera/genética
2.
J Biol Chem ; 297(4): 101203, 2021 10.
Artículo en Inglés | MEDLINE | ID: mdl-34537243

RESUMEN

Aminoacyl-tRNA synthetases (ARSs) catalyze the charging of specific amino acids onto cognate tRNAs, an essential process for protein synthesis. Mutations in ARSs are frequently associated with a variety of human diseases. The human EPRS1 gene encodes a bifunctional glutamyl-prolyl-tRNA synthetase (EPRS) with two catalytic cores and appended domains that contribute to nontranslational functions. In this study, we report compound heterozygous mutations in EPRS1, which lead to amino acid substitutions P14R and E205G in two patients with diabetes and bone diseases. While neither mutation affects tRNA binding or association of EPRS with the multisynthetase complex, E205G in the glutamyl-tRNA synthetase (ERS) region of EPRS is defective in amino acid activation and tRNAGlu charging. The P14R mutation induces a conformational change and altered tRNA charging kinetics in vitro. We propose that the altered catalytic activity and conformational changes in the EPRS variants sensitize patient cells to stress, triggering an increased integrated stress response (ISR) that diminishes cell viability. Indeed, patient-derived cells expressing the compound heterozygous EPRS show heightened induction of the ISR, suggestive of disruptions in protein homeostasis. These results have important implications for understanding ARS-associated human disease mechanisms and development of new therapeutics.


Asunto(s)
Enfermedades Óseas , Diabetes Mellitus , Enfermedades Genéticas Congénitas , Glutamato-ARNt Ligasa , Mutación Missense , Estrés Fisiológico/genética , Sustitución de Aminoácidos , Enfermedades Óseas/enzimología , Enfermedades Óseas/genética , Diabetes Mellitus/enzimología , Diabetes Mellitus/genética , Enfermedades Genéticas Congénitas/enzimología , Enfermedades Genéticas Congénitas/genética , Glutamato-ARNt Ligasa/química , Glutamato-ARNt Ligasa/genética , Glutamato-ARNt Ligasa/metabolismo , Células HEK293 , Humanos , Masculino
3.
J Biol Chem ; 289(21): 15023-34, 2014 May 23.
Artículo en Inglés | MEDLINE | ID: mdl-24719324

RESUMEN

In response to amino acid starvation, GCN2 phosphorylation of eIF2 leads to repression of general translation and initiation of gene reprogramming that facilitates adaptation to nutrient stress. GCN2 is a multidomain protein with key regulatory domains that directly monitor uncharged tRNAs which accumulate during nutrient limitation, leading to activation of this eIF2 kinase and translational control. A critical feature of regulation of this stress response kinase is its C-terminal domain (CTD). Here, we present high resolution crystal structures of murine and yeast CTDs, which guide a functional analysis of the mammalian GCN2. Despite low sequence identity, both yeast and mammalian CTDs share a core subunit structure and an unusual interdigitated dimeric form, albeit with significant differences. Disruption of the dimeric form of murine CTD led to loss of translational control by GCN2, suggesting that dimerization is critical for function as is true for yeast GCN2. However, although both CTDs bind single- and double-stranded RNA, murine GCN2 does not appear to stably associate with the ribosome, whereas yeast GCN2 does. This finding suggests that there are key regulatory differences between yeast and mammalian CTDs, which is consistent with structural differences.


Asunto(s)
Multimerización de Proteína , Proteínas Serina-Treonina Quinasas/química , Estructura Terciaria de Proteína , Proteínas de Saccharomyces cerevisiae/química , Animales , Células Cultivadas , Cristalización , Cristalografía por Rayos X , Embrión de Mamíferos/citología , Fibroblastos/citología , Fibroblastos/metabolismo , Immunoblotting , Ratones , Ratones Noqueados , Modelos Moleculares , Mutación , Unión Proteica , Biosíntesis de Proteínas/genética , Proteínas Serina-Treonina Quinasas/genética , Proteínas Serina-Treonina Quinasas/metabolismo , ARN/química , ARN/genética , ARN/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo
4.
Mol Biol Cell ; 22(22): 4390-405, 2011 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-21917591

RESUMEN

Disruptions of the endoplasmic reticulum (ER) that perturb protein folding cause ER stress and elicit an unfolded protein response (UPR) that involves translational and transcriptional changes in gene expression aimed at expanding the ER processing capacity and alleviating cellular injury. Three ER stress sensors (PERK, ATF6, and IRE1) implement the UPR. PERK phosphorylation of the α subunit of eIF2 during ER stress represses protein synthesis, which prevents further influx of ER client proteins. Phosphorylation of eIF2α (eIF2α~P) also induces preferential translation of ATF4, a transcription activator of the integrated stress response. In this study we show that the PERK/eIF2α~P/ATF4 pathway is required not only for translational control, but also for activation of ATF6 and its target genes. The PERK pathway facilitates both the synthesis of ATF6 and trafficking of ATF6 from the ER to the Golgi for intramembrane proteolysis and activation of ATF6. As a consequence, liver-specific depletion of PERK significantly reduces both the translational and transcriptional phases of the UPR, leading to reduced protein chaperone expression, disruptions of lipid metabolism, and enhanced apoptosis. These findings show that the regulatory networks of the UPR are fully integrated and help explain the diverse biological defects associated with loss of PERK.


Asunto(s)
Factor de Transcripción Activador 6/metabolismo , Estrés del Retículo Endoplásmico , Retículo Endoplásmico/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Respuesta de Proteína Desplegada , eIF-2 Quinasa/metabolismo , Factor de Transcripción Activador 4/metabolismo , Animales , Apoptosis , Proteínas Portadoras/metabolismo , Células Cultivadas , Factor 2 Eucariótico de Iniciación/metabolismo , Aparato de Golgi/metabolismo , Metabolismo de los Lípidos , Proteínas de la Membrana/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Chaperonas Moleculares/metabolismo , Fosforilación , Pliegue de Proteína , Procesamiento Proteico-Postraduccional , Transporte de Proteínas , Transcripción Genética
5.
J Biol Chem ; 284(37): 25254-67, 2009 Sep 11.
Artículo en Inglés | MEDLINE | ID: mdl-19546227

RESUMEN

When cells are subjected to nutritional stress, uncharged tRNAs accumulate and activate Gcn2p phosphorylation of eukaryotic initiation factor-2 (eIF2) and the general amino acid control pathway. The Gcn2p regulatory domain homologous to histidyl-tRNA synthetases is proposed to bind to uncharged tRNA, directly contributing to activation of Gcn2p. Here we apply a microarray technology to analyze genome-wide changes in tRNA charging in yeast upon activation of Gcn2p in response to amino acid starvation and high salinity, a stress not directly linked to nutritional deficiency. This microarray technology is applicable for all eukaryotic cells. Strains were starved for histidine, leucine, or tryptophan and shown to rapidly induce Gcn2p phosphorylation of eIF2. The relative charging level of all tRNAs was measured before and after starvation, and Gcn2p activation and the intracellular levels of the starved amino acid correlate with the observed decrease in tRNA charging. Interestingly, in some cases, tRNAs not charged with the starved amino acid became deacylated more rapidly than tRNAs charged with the starved amino acid. This increase in uncharged tRNA levels occurred although the intracellular levels for these non-starved amino acids remained unchanged. Additionally, treatment of a wild-type strain with high salinity stress showed transient changes in the charging of several different tRNAs. These results suggest that Gcn2p can be activated by many different tRNA species in the cell. These results also depict a complex cellular relationship between tRNA charging, amino acid availability, and non-nutrient stress. These relationships are best revealed by simultaneous monitoring of the charging level of all tRNAs.


Asunto(s)
Genoma Fúngico , Proteínas Serina-Treonina Quinasas/fisiología , Proteínas de Saccharomyces cerevisiae/fisiología , eIF-2 Quinasa/metabolismo , Aminoácidos/química , Northern Blotting , Factor 2 Eucariótico de Iniciación/metabolismo , Immunoblotting , Leucina/química , Fosforilación , Proteínas Serina-Treonina Quinasas/genética , ARN de Transferencia/química , Proteínas de Saccharomyces cerevisiae/genética , Cloruro de Sodio/farmacología , Factores de Tiempo , Triptófano/química
6.
J Biol Chem ; 281(42): 31222-33, 2006 Oct 20.
Artículo en Inglés | MEDLINE | ID: mdl-16931516

RESUMEN

L-asparaginase is important in the induction regimen for treating acute lymphoblastic leukemia. Cytotoxic complications are clinically significant problems lacking mechanistic insight. To reveal tissue-specific molecular responses to this drug, mice were administered asparaginase from either Escherichia coli (clinically used) or Wolinella succinogenes (novel, glutaminase-free form). Both enzymes abolished serum asparagine, but only the E. coli form reduced circulating glutamine. E. coli asparaginase reduced protein synthesis in liver and spleen but not pancreas via increased phosphorylation of the translation factor eIF2. In contrast, treatment with Wolinella caused no untoward changes in protein synthesis in any tissue examined. Treating mice deleted for the eIF2 kinase, GCN2, with the E. coli enzyme showed eIF2 phosphorylation to be GCN2-dependent, but only initially. Furthermore, although eIF2 phosphorylation was not increased in the pancreas or by Wolinella asparaginase, expression of the amino acid stress response genes, asparagine synthetase and CHOP/GADD153, increased as a result of both enzymes, even in tissues demonstrating no change in eIF2 phosphorylation. Finally, signaling downstream of the mammalian target of rapamycin kinase was repressed in liver and pancreas by E. coli but not Wolinella asparaginase. These data demonstrate that the nutrient stress response to asparaginase is tissue-specific and exacerbated by glutamine depletion. Importantly, increased expression of asparagine synthetase and CHOP does not require eIF2 phosphorylation, signifying alternate or auxiliary means of inducing gene expression under conditions of amino acid depletion in the whole animal.


Asunto(s)
Asparaginasa/farmacología , Glutamina/metabolismo , Neoplasias Experimentales/terapia , Animales , Asparagina/sangre , Escherichia coli/enzimología , Femenino , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Neoplasias Experimentales/tratamiento farmacológico , Proteínas Serina-Treonina Quinasas/metabolismo , Wolinella/enzimología
7.
Mol Cell Biol ; 24(3): 1365-77, 2004 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-14729979

RESUMEN

In response to environmental stress, cells induce a program of gene expression designed to remedy cellular damage or, alternatively, induce apoptosis. In this report, we explore the role of a family of protein kinases that phosphorylate eukaryotic initiation factor 2 (eIF2) in coordinating stress gene responses. We find that expression of activating transcription factor 3 (ATF3), a member of the ATF/CREB subfamily of basic-region leucine zipper (bZIP) proteins, is induced in response to endoplasmic reticulum (ER) stress or amino acid starvation by a mechanism requiring eIF2 kinases PEK (Perk or EIF2AK3) and GCN2 (EIF2AK4), respectively. Increased expression of ATF3 protein occurs early in response to stress by a mechanism requiring the related bZIP transcriptional regulator ATF4. ATF3 contributes to induction of the CHOP transcriptional factor in response to amino acid starvation, and loss of ATF3 function significantly lowers stress-induced expression of GADD34, an eIF2 protein phosphatase regulatory subunit implicated in feedback control of the eIF2 kinase stress response. Overexpression of ATF3 in mouse embryo fibroblasts partially bypasses the requirement for PEK for induction of GADD34 in response to ER stress, further supporting the idea that ATF3 functions directly or indirectly as a transcriptional activator of genes targeted by the eIF2 kinase stress pathway. These results indicate that ATF3 has an integral role in the coordinate gene expression induced by eIF2 kinases. Given that ATF3 is induced by a very large number of environmental insults, this study supports involvement of eIF2 kinases in the coordination of gene expression in response to a more diverse set of stress conditions than previously proposed.


Asunto(s)
Factor 2 Eucariótico de Iniciación/metabolismo , Fosfotransferasas/metabolismo , Factores de Transcripción/metabolismo , eIF-2 Quinasa/metabolismo , Factor de Transcripción Activador 3 , Factor de Transcripción Activador 4 , Animales , Proteínas Potenciadoras de Unión a CCAAT/metabolismo , Ratones , Fosforilación , Proteínas Quinasas/metabolismo , Proteínas Serina-Treonina Quinasas , ARN Mensajero/metabolismo , Factor de Transcripción CHOP
8.
Mol Cell Biol ; 23(16): 5651-63, 2003 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-12897138

RESUMEN

Nuclear factor kappaB (NF-kappaB) serves to coordinate the transcription of genes in response to diverse environmental stresses. In this report we show that phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2) is fundamental to the process by which many stress signals activate NF-kappaB. Phosphorylation of this translation factor is carried out by a family of protein kinases that each respond to distinct stress conditions. During impaired protein folding and assembly in the endoplasmic reticulum (ER), phosphorylation of eIF2alpha by PEK (Perk or EIF2AK3) is essential for induction of NF-kappaB transcriptional activity. The mechanism by which NF-kappaB is activated during ER stress entails the release, but not the degradation, of the inhibitory protein IkappaB. During amino acid deprivation, phosphorylation of eIF2alpha by GCN2 (EIF2AK4) signals the activation of NF-kappaB. Furthermore, inhibition of general translation or transcription by cycloheximide and actinomycin D, respectively, elicits the eIF2alpha phosphorylation required for induction of NF-kappaB. Together, these studies suggest that eIF2alpha kinases monitor and are activated by a range of stress conditions that affect transcription and protein synthesis and assembly, and the resulting eIFalpha phosphorylation is central to activation of the NF-kappaB. The absence of NF-kappaB-mediated transcription and its antiapoptotic function provides an explanation for why eIF2alpha kinase deficiency in diseases such as Wolcott-Rallison syndrome leads to cellular apoptosis and disease.


Asunto(s)
Factor 2 Eucariótico de Iniciación/fisiología , Fosforilación , Animales , Apoptosis , Núcleo Celular/metabolismo , Células Cultivadas , Cicloheximida/farmacología , Dactinomicina/farmacología , Relación Dosis-Respuesta a Droga , Retículo Endoplásmico/metabolismo , Activación Enzimática , Factor 2 Eucariótico de Iniciación/metabolismo , Heterocigoto , Immunoblotting , Luciferasas/metabolismo , Ratones , Microscopía Confocal , Microscopía Fluorescente , FN-kappa B/metabolismo , Biosíntesis de Proteínas , Inhibidores de la Síntesis de la Proteína/farmacología , Transporte de Proteínas , Tapsigargina/farmacología , Transcripción Genética , Activación Transcripcional
9.
Mol Cell Biol ; 22(19): 6681-8, 2002 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-12215525

RESUMEN

The GCN2 eIF2alpha kinase is essential for activation of the general amino acid control pathway in yeast when one or more amino acids become limiting for growth. GCN2's function in mammals is unknown, but must differ, since mammals, unlike yeast, can synthesize only half of the standard 20 amino acids. To investigate the function of mammalian GCN2, we have generated a Gcn2(-/-) knockout strain of mice. Gcn2(-/-) mice are viable, fertile, and exhibit no phenotypic abnormalities under standard growth conditions. However, prenatal and neonatal mortalities are significantly increased in Gcn2(-/-) mice whose mothers were reared on leucine-, tryptophan-, or glycine-deficient diets during gestation. Leucine deprivation produced the most pronounced effect, with a 63% reduction in the expected number of viable neonatal mice. Cultured embryonic stem cells derived from Gcn2(-/-) mice failed to show the normal induction of eIF2alpha phosphorylation in cells deprived of leucine. To assess the biochemical effects of the loss of GCN2 in the whole animal, liver perfusion experiments were conducted. Histidine limitation in the presence of histidinol induced a twofold increase in the phosphorylation of eIF2alpha and a concomitant reduction in eIF2B activity in perfused livers from wild-type mice, but no changes in livers from Gcn2(-/-) mice.


Asunto(s)
Adaptación Fisiológica/fisiología , Factor 2 Eucariótico de Iniciación/metabolismo , Glicina/deficiencia , Leucina/deficiencia , Proteínas Quinasas/deficiencia , Triptófano/deficiencia , Animales , Animales Recién Nacidos , Células Cultivadas , Factor 2B Eucariótico de Iniciación/metabolismo , Femenino , Viabilidad Fetal/genética , Alimentos Formulados , Regulación de la Expresión Génica , Marcación de Gen , Heterocigoto , Homocigoto , Hígado/metabolismo , Ratones , Ratones Noqueados , Fosforilación , Embarazo , Efectos Tardíos de la Exposición Prenatal , Proteínas Quinasas/genética , Proteínas Quinasas/metabolismo , Proteínas Serina-Treonina Quinasas , Subunidades de Proteína , Células Madre/citología , Células Madre/metabolismo
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