Effect of soybean proteins and peptides on the growth and adhesive ability of Limosilactobacillus Reuteri DSM17938.

Limosilactobacillus reuteri DSM17938 is one of the most pivotal probiotics, whose general beneficial effects on the intestinal microbiota are well recognized. Enhancing their growth and metabolic activity can effectively regulate the equilibrium of intestinal microbiota, leading to improved physical health. A common method to promote the growth of Lactobacillus is the addition of prebiotics. Current research suggests that proteins and their hydrolysates from different sources with potential prebiotic activity can also promote the growth of probiotics. In this study, soybean proteins and peptides were effective in promoting the growth, organic acid secretion, and adhesive properties of Limosilactobacillus reuteri DSM17938 to Caco-2 cells. These results illustrate the feasibility of soybean proteins and peptides as prebiotics, providing theoretical and practical advantages for their application.

A review of common statistical methods for dealing with multiple pollutant mixtures and multiple exposures.

Traditional environmental epidemiology has consistently focused on studying the impact of single exposures on specific health outcomes, considering concurrent exposures as variables to be controlled. However, with the continuous changes in environment, humans are increasingly facing more complex exposures to multi-pollutant mixtures. In this context, accurately assessing the impact of multi-pollutant mixtures on health has become a central concern in current environmental research. Simultaneously, the continuous development and optimization of statistical methods offer robust support for handling large datasets, strengthening the capability to conduct in-depth research on the effects of multiple exposures on health. In order to examine complicated exposure mixtures, we introduce commonly used statistical methods and their developments, such as weighted quantile sum, bayesian kernel machine regression, toxic equivalency analysis, and others. Delineating their applications, advantages, weaknesses, and interpretability of results. It also provides guidance for researchers involved in studying multi-pollutant mixtures, aiding them in selecting appropriate statistical methods and utilizing R software for more accurate and comprehensive assessments of the impact of multi-pollutant mixtures on human health.

Effect modification of diet and vitamins on the association between air pollution particles of different diameters and hypertension: A 12-year longitudinal cohort study in densely populated areas of China.

INTRODUCTION: Particulate matter (PM) is identified as one of the exacerbating and triggering factors for hypertension. Diet intake and the consumption of vitamins may potentially moderate the impact of PM on hypertension. METHODS: A 12-year longitudinal cohort study was conducted on a population in densely populated areas of China. Residual balancing with weighted methods was employed to control for time-varying and no time-varying confounding factors. Stratified Cox proportional hazards models were conducted to examine the moderating effects of diet and vitamins on the risk of hypertension with PM. RESULTS: There was a significant positive association between long-term exposure to different diameter PM and the risk of developing hypertension. The hazard ratios (HRs) for hypertension were 1.0200 (95 % CIs: 1.0147, 1.0253) for PM1, 1.0120 (95 % CIs: 1.0085, 1.0155) for PM2.5, and 1.0074 (95 % CIs, 1.0056, 1.0092) for PM10. The diet and vitamins moderated these associations, the intake of healthy foods and vitamins exhibited a significant positive moderating effect on the relationship between PM exposure and hypertension risk. Among all participants, the high intake of fruit (PM1 (HRs: 1.0102, 95 % CIs: 1.0024, 1.0179), PM2.5 (HRs: 1.0060, 95 % CIs: 1.0011, 1.0109), and PM10 (HRs: 1.0044, 95 % CIs: 1.0018, 1.0070)) and vitamin E (PM1 (HRs: 1.0143, 95 % CIs: 1.0063, 1.0223), PM2.5 (HRs:1.0179, 95 % CIs: 1.0003, 1.0166), and PM10 (HRs: 1.0042, 95 % CIs: 1.0008, 1.0075)) with lower risk of hypertension than the overall level and low intake of related foods and vitamins, exhibited a strong positive moderating effect on the relationship between PM and hypertension. Similar trends were observed for the intake of fish, root food, whole grains, eggs, fungus food, vitamin B2, B3. However, Na, meat, sugary and alcoholic exhibited opposite trends. The moderating effect of vitamin E intake was stronger than vitamin B and C. CONCLUSIONS: Diet and vitamins intake may moderate the association between PM exposure and the risk of hypertension in adults.

Investigating the antibacterial mode of Limosilactobacillus reuteri LR08 regulated by soybean proteins and peptides.

Soybean proteins (pro) and soybean peptides (pep) are beneficial to the growth and metabolism of Limosilactobacillus reuteri (L. reuteri). However, whether they could assist L. reuteri in inhibiting intestinal pathogens and the inhibition mode of them is still unclear. In this study, a co-culture experiment of L. reuteri LR08 with Escherichia coli JCM 1649 (E. coli) was performed. It showed that pro and pep could still favour the growth of L. reuteri over E. coli under their competition. The inhibition zone experiment showed the digested soybean proteins (dpro) could improve its antibacterial activity by increasing the secretion of organic acids from L. reuteri. Furthermore, digested soybean peptides (dpep) could enhance nitrogen utilization capacity of L. reuteri over E. coli. These results explained the patterns of dpro and dpep assisting L. reuteri in inhibiting the growth of E. coli by regulating its organic acid secretion and the ability of nitrogen utilization.

The investigation of soybean protein isolates and soybean peptides assisting Lactobacillus plantarum K25 to inhibit Escherichia coli.

Soybean protein isolates and their hydrolysates are considered as one of the most high-quality proteins among plant proteins, and current research has shown that they have potential probiotic functions. The purpose of this study was to investigate the effects of digested soybean protein isolates (dSPI) and digested soybean peptides (dPEP) on L. plantarum K25 alone and the two bacteria when co-cultured with E. coli. It showed that dSPI and dPEP promoted the growth and metabolism of L. plantarum K25, and dSPI had a better effect. Besides, dSPI and dPEP still promoted the growth and organic acid secretion of L. plantarum K25 when co-cultured with E. coli, and the dPEP treatment was more effective than dSPI. Moreover, dSPI and dPEP reduced the survival rate of E. coli when co-cultured with L. plantarum K25. These results to some extent explained the cooperation of dSPI and dPEP with L. plantarum K25 to produce acid thereby weaken the growth of E. coli.

Genetic evidence for the causal relations between metabolic syndrome and psychiatric disorders: a Mendelian randomization study.

Emerging evidence reveals associations between metabolic syndrome (MetS) and psychiatric disorders (PDs), although causality remains uncertain. Consequently, we conducted Mendelian randomization (MR) to systematically evaluate the causality between MetS and PDs. Linkage disequilibrium score regression estimated the heritability of PDs and their genetic correlations with MetS. In primary analyses, the main model employed inverse variance weighting method, with sensitivity analyses using various MR models to ensure robustness. Replication MR analyses, involving cohorts distinct from those in the primary analyses, were performed to validate the generalizability of the findings. Multivariable MR analyses were carried out to account for genetically predicted body mass index (BMI). As a result, genetic correlations of MetS with attention-deficit/hyperactivity disorder(ADHD), anorexia nervosa(ANO), major depressive disorder(MDD), and schizophrenia were identified. Causal effects of MetS on ADHD (OR: 1.59 [95% CI:1.45-1.74]), ANO (OR: 1.42 [95% CI:1.25-1.61]), MDD(OR: 1.23 [95% CI: 1.13-1.33]), and the effects of ADHD (OR: 1.03 [95% CI: 1.02-1.04]) and ANO (OR: 1.01 [95% CI: 1.01-1.02]) on MetS were observed in primary analyses. Results from sensitivity analyses and replication analyses were generally consistent with the primary analyses, confirming the robustness and generalizability of the findings. Associations between MetS and ADHD, as well as ANO persisted after adjusting for BMI, whereas the statistical significance of the association between MetS and MDD was no longer observable. These results contribute to a deeper understanding of the mechanisms underlying PDs, suggesting potential modifiable targets for public prevention and clinical intervention in specific PDs related to metabolic pathways.

Can proteins, protein hydrolysates and peptides cooperate with probiotics to inhibit pathogens?

Studies have shown that probiotics can effectively inhibit pathogens in the presence of proteins, protein hydrolysates and peptides (protein derivates). However, it is still unclear the modes of probiotics to inhibit pathogens regulated by protein derivates. Therefore, we summarized the possible effects of protein derivates from different sources on probiotics and pathogens. There is abundant evidence that proteins and peptides from different sources can significantly promote the proliferation of probiotics and increase their secretion of antibacterial substances. Such proteins and peptides can also stimulate the adhesion of probiotics to intestinal epithelial cells and contribute to regulating intestinal immunity, but they seem to have the negative effects on pathogens. Moreover, a direct effect of proteins on intestinal cells is summarized. Whether or not they can cooperate with probiotics to inhibit pathogens using above possible mechanisms were discussed. Furthermore, there seems to be no consistent conclusions that protein derivates have synergistic effects with probiotics, and there is still limited evidence on the inhibiting patterns. Therefore, the existing problems and shortcomings are noted, and future research direction is proposed.

Global border restrictions in 2020-2021: Adherence and the effectiveness in long-term COVID-19 epidemic control.

BACKGROUND: Restrictions on international travel were widely applied to contain cross-border COVID-19 diffusion, while such applications varied globally, and little was known about their impacts on the long-term epidemic progression. METHODS: We explored the global diversity in maintaining border policies classified to four levels (screening, quarantine, ban on regions and total border closure) using data of 185 countries and regions between 01 January 2020 to 31 December 2021. By using Ordinary least squares (OLS) regression and quantile regression (QR) models, we examined the relationship between total COVID-19 incidence and the cumulative duration of each policy level in 2020-2021, and the heterogeneity of such association across different transmission severity countries. RESULTS: Firstly, "ban on regions" was the most durable policy applied in high-income countries, while in low-income countries, less stringent measures of screening and quarantine arrivals were applied the longest. Secondly, the cumulatively longer maintenance of the border quarantine was significantly associated with lower infections (log) in COVID-19 high-prevalent countries (75th QR, coefficient estimates [ß] = -0.0038, 95% confidence interval: -0.0066 to -0.0010). By contrast, in medium and high transmission severity countries, those with longer duration of imposing bans on regions showed no suppressing effects but significantly higher COVID-19 incidence (OLS regression, ß = 0.0028, 95% CI: 0.0009-0.0047; 75th QR, ß = 0.0039, 95% CI: 0.0014-0.0063). No other significant results were found. CONCLUSION: From the long-term perspective, inbound quarantine was effective in mitigating severe epidemics. However, in countries with medium or high COVID-19 prevalence, our findings of ban on regions highlighted its ineffectiveness in the long-term epidemic progression.