Effects of comorbidities on quality of life in Filipino people with tuberculosis.

BACKGROUND: We investigated health-related quality of life (HrQoL) in Filipino people undergoing TB treatment, and whether HrQoL was negatively impacted by comorbidity with undernutrition, diabetes (DM) and anaemia.METHODS: Adult participants were enrolled in public facilities in Metro Manila (three sites) and Negros Occidental (two sites). Multivariate linear regression was used to model the four correlated domain scores from a WHOQOL-BREF questionnaire (physical, psychological, social, environmental). A forward-stepwise approach was used to select a final multivariable model with inclusion based on global tests of significance at P < 0.1.RESULTS: In 446 people on drug-susceptible TB treatment, DM and moderate/severe anaemia were not associated with HrQoL. After adjustment for age, sex, education, food insecurity, treatment adherence, inflammation, Category I or II TB treatment, treatment phase, current side effects and inhibited ability to work, moderate/severe undernutrition (body mass index < 17 kg/m²) was associated with lower HrQoL (P = 0.003) with reduced psychological (coefficient: -1.02, 95% CI -1.54 to -0.51), physical (-0.62, 95% CI -1.14 to -0.09) and environmental domain scores (-0.45, 95% CI -0.88 to -0.01). In 225 patients with known HIV status in Metro Manila, HIV was associated with modestly reduced HrQoL (P = 0.014).CONCLUSION: Nutritional status and food insecurity represent modifiable risk factors for poor HrQoL that may be alleviated through interventions.

Analysis of menisci formed on cones for single field of view parasite egg microscopy.

Parasite ova caused to accumulate in a single microscopic field simplifies monitoring soil-transmitted helminthiasis by optical microscopy. Here we demonstrate new egg-accumulating geometries based on annular menisci formed on the surface of a wetted cone. Fluidic features extracted from profile images of the system provided mathematical representations of the meniscus gradient that were compared quantitatively to numerical solutions of an axisymmetric Young-Laplace equation. Our results show that the governing dynamics of these systems is dominated by the surface tension of the fluid. These image analysis and mathematical tools provide simple quantitative methods for system analysis and optimization.

Quantitative particle microscopy in self-metered fluids.

We describe a simple device that volumetrically samples poured liquids and draws buoyant materials into a single field of view for quantitative particle microscopy. Our approach relies on the formation of axisymmetric menisci and computational models of the static fluid developed using surface evolver showed close agreement with experiment. The apparatus was evaluated by counting pollen and demonstrated utility for the field analysis of microparticles.

The use of antimicrobial drugs in Nagpur, India. A window on medical care in a developing country.

The objective of the study was to determine the patterns of use of antimicrobial drugs in the general population of the large, industrial city of Nagpur, India. Interviews of pharmacists and clients were carried out in a stratified, random sample of 34 pharmacies to determine beliefs and practices in prescribing and self-prescribing of antibiotics by complaint, choice of drug, dose, duration, cost, age and sex of the consumers. The study showed that drugs were dispensed without prescription despite prohibition by the Indian Pharmaceutical Act. Sales of antimicrobial drugs accounted for 17.5% of 511 purchases and 23.3% of expenditures for drugs. Proprietary brands of penicillins, co-trimoxazole and tetracyclines were dispensed most often (64.8%). The most common indications were upper respiratory, gastrointestinal and nonspecific complaints. The median number of units obtained was 5.0 (95% range 1-20), at a median cost of $0.50 per purchase, usually taken for less than five days. Repeat purchases were made without consulting a physician. Almost two thirds of purchases (63.9%) were for males, mainly under the age of ten years. Clients had poor knowledge of the indications, side effects, adverse reactions and appropriate duration of therapy. The dispenser viewed himself as a businessman rather than a professional and rarely offered unsolicited advice. Co-prescribing of 'tonics' added to costs and decreased the purchasing power for antimicrobial drugs. Most purchases of antimicrobial drugs in community pharmacies in Nagpur were for minor indications and were limited by the purchasing power of the consumers. It is doubtful that the choice of drug and the short duration of therapy would be effective for serious infections.(ABSTRACT TRUNCATED AT 250 WORDS)

A reassessment of the importance of "low-count" bacteriuria in young women with acute urinary symptoms.

OBJECTIVE: To determine whether a statistical association exists between "low-count" bacteriuria (> 10(2) to 10(4) colony-forming units/mL) and acute urinary symptoms in young women. DESIGN: Prospective, case-control study. SETTING: Gynecology clinic at a student health center. PATIENTS: Women with or without urinary or vaginal symptoms. MEASUREMENTS: History of urinary infections and sexual activity. Quantitative determination of bacteriuria and pyuria and bacterial species; urine leukocyte esterase test; specific gravity; creatinine levels; vaginal leukocytes; and in-vitro culture of urine. RESULTS: The frequency of recent sexual activity, pregnancies, and contraceptive practices was not statistically different between women with acute urinary symptoms and asymptomatic controls. Escherichia coli and Staphylococcus saprophyticus were the only microorganisms statistically associated with urinary symptoms and pyuria (P < 0.001). Low counts of these organisms were found in 10.2% of asymptomatic women. As the bacterial count increased, the association between these organisms and symptoms increased, and a step-wise increase occurred in the frequency and magnitude of pyuria, but the specific gravity and urine creatinine levels remained unchanged. Escherichia coli, even at low counts, grew well in the patients' own urine. Pyuria (> 20 leukocytes/mm3) was present in 19.6% of asymptomatic women and was associated with vaginal leukorrhea. CONCLUSIONS: "Low-count" bacteriuria was statistically more frequent among young women with urinary symptoms than among asymptomatic controls. The low counts could not be explained by dilution of the urine or failure of the bacteria to grow well in the patients' urine. These findings suggest that the infection was not established in the bladder urine and that "low-count" bacteriuria might be an early phase of urinary tract infection.

Do temperature-sensitive auxotrophs of Escherichia coli have special virulence?

To determine whether temperature (42 degrees C)-sensitive auxotrophs of Escherichia coli have special virulence properties (W. D. Welch, D. Kitts, H. S. Moyed, and L. D. Thrupp, J. Clin. Microbiol. 13:606-608, 1981), we examined 301 strains isolated from patients with bacteremia or acute cystitis and from the stools of healthy subjects. Of these strains, 49.5% grew at 42 degrees C without supplements, 39.2% required a nutritional supplement, and 11.3% failed to grow even with selected nutrients. Nicotinamide restored growth for 35.2% of strains at either 37 or 42 degrees C. Some of strains required methionine, glutamic, aspartic, and amino acid mixtures or NaCl for growth at 42 degrees C. Temperature-sensitive strains were significantly more abundant in isolates from blood and urine than in stool, but temperature-sensitive auxotrophs were isolated at about the same frequency from each site. There were no discernible clonal patterns, by serotype, among of the nicotinamide-requiring temperature-sensitive auxotrophs. Resistance to trimethoprim-sulfamethoxazole was associated with ability to grow at 42 degrees C. This was not observed with any other antimicrobial drug. Temperature-sensitive strains are a heterogenous group. The relationship of temperature-sensitive auxotrophy to virulence is uncertain.

Dispersion, Dissipation, and Efficacy of Methyl Bromide-Chloropicrin Gas vs. Gel Formulations on Nematodes and Weeds in Tifton Sandy Loam.

Dispersion, dissipation, and efficacy of gas and gel formulations of methyl bromidechloropicrin (202, 269, 336, and 403 kg/ha) on nematodes and weeds on tomato were studied in field plots. Concentrations of methyl bromide and chloropicrin 4 hr after soil treatment were greater at a depth of 15 cm than at 30, 45, or 60 cm. The concentrations of both chemicals decreased with lower doses, greater depths, and longer times after application. The gel formulation was more persistent than the gas formulation at both 336 and 403 kg/ha at depths of 30 and 45 cm, especially 24 and 36 hr after chemical application. Plant growth and yield were improved when nematodes and weeds were controlled.