Improved algorithm for the transmittance estimation of spectra obtained with SOIR/Venus Express.

The Solar Occultation in the InfraRed (SOIR) instrument onboard the ESA Venus Express spacecraft, an infrared spectrometer sensitive from 2.2 to 4.3 µm, probed the atmosphere of Venus from June 2006 until December 2014. During this time, it performed more than 750 solar occultations of the Venus mesosphere and lower thermosphere. A new procedure has been developed for the estimation of the transmittance in order to decrease the number of rejected spectra, to check that the treated spectra are well calibrated, and to improve the quality of the calibrated spectra by reducing the noise and accurately normalizing it to the solar spectrum.

Improved calibration of SOIR/Venus Express spectra.

The SOIR instrument on board the ESA Venus Express mission has been operational since the insertion of the satellite around Venus in April 2006. Since then, it has delivered high quality IR solar occultation spectra of the atmosphere of Venus. The different steps from raw spectra to archived data are described and explained in detail here. These consist of corrections for the dark current and for the non-linearity of the detector; removing bad pixels, as well as deriving noise. The spectral calibration procedure is described, along with all ancillary data necessary for the understanding and interpretation of the SOIR data. These include the full characterization of the AOTF filter, one of the major elements of the instrument. All these data can be found in the ESA PSA archive.

In-flight performance and calibration of SPICAV SOIR onboard Venus Express.

Solar occultation in the infrared, part of the Spectoscopy for Investigation of Characteristics of the Atmosphere of Venus (SPICAV) instrument onboard Venus Express, combines an echelle grating spectrometer with an acousto-optic tunable filter (AOTF). It performs solar occultation measurements in the IR region at high spectral resolution. The wavelength range probed allows a detailed chemical inventory of Venus's atmosphere above the cloud layer, highlighting the vertical distribution of gases. A general description of the instrument and its in-flight performance is given. Different calibrations and data corrections are investigated, in particular the dark current and thermal background, the nonlinearity and pixel-to-pixel variability of the detector, the sensitivity of the instrument, the AOTF properties, and the spectral calibration and resolution.

Amyloid-beta precursor protein processing in neurodegeneration.

The amyloid-beta precursor protein is proteolytically cleaved by secretases, resulting in a series of fragments, including the amyloid-beta peptide of Alzheimer's disease. The amyloid precursor protein, when membrane anchored, could operate as a receptor. After cleavage, the soluble ectodomain exerts a trophic function in the subventricular zone. The amyloid-beta peptide itself has a depressant role in synaptic transmission, with both physiological and pathological implications. During the past two years, much time has been invested in determining the molecular pathways that regulate the processing and the signal transduction of the amyloid precursor protein. However, the absence of consistent and informative phenotypes in different loss of function animal models make elucidating the molecular actions of the amyloid-beta precursor protein an ongoing challenge.

Chromosomal anomalies in individuals with autism: a strategy towards the identification of genes involved in autism.

We review the different strategies currently used to try to identify susceptibility genes for idiopathic autism. Although identification of genes is usually straightforward in Mendelian disorders, it has proved to be much more difficult to establish in polygenic disorders like autism. Neither genome screens of affected siblings nor the large number of association studies using candidate genes have resulted in finding autism susceptibility genes. We focus on the alternative approach of 'positional cloning' through chromosomal aberrations in individuals with autism. In particular, balanced aberrations such as reciprocal translocations or inversions offer a unique opportunity, since only the genes in the breakpoint regions are candidate genes. This approach, in combination with others, is likely to produce results in the coming years.

Dihydropteridine reductase as an alternative to dihydrofolate reductase for synthesis of tetrahydrofolate in Thermus thermophilus.

A strategy devised to isolate a gene coding for a dihydrofolate reductase from Thermus thermophilus DNA delivered only clones harboring instead a gene (the T. thermophilus dehydrogenase [DH(Tt)] gene) coding for a dihydropteridine reductase which displays considerable dihydrofolate reductase activity (about 20% of the activity detected with 6,7-dimethyl-7,8-dihydropterine in the quinonoid form as a substrate). DH(Tt) appears to account for the synthesis of tetrahydrofolate in this bacterium, since a classical dihydrofolate reductase gene could not be found in the recently determined genome nucleotide sequence (A. Henne, personal communication). The derived amino acid sequence displays most of the highly conserved cofactor and active-site residues present in enzymes of the short-chain dehydrogenase/reductase family. The enzyme has no pteridine-independent oxidoreductase activity, in contrast to Escherichia coli dihydropteridine reductase, and thus appears more similar to mammalian dihydropteridine reductases, which do not contain a flavin prosthetic group. We suggest that bifunctional dihydropteridine reductases may be responsible for the synthesis of tetrahydrofolate in other bacteria, as well as archaea, that have been reported to lack a classical dihydrofolate reductase but for which possible substitutes have not yet been identified.