RESUMEN
The genomes of Plasmodium parasites encode for five perforin-like proteins, PPLP1-5, and four of them have previously been demonstrated to be involved in disruption of host cell barriers. We now show that the fifth perforin, PPLP4, is crucial for infection of the mosquito vector by Plasmodium falciparum parasites. PPLP4 is expressed in the blood and mosquito midgut stages in granular structures. In gametocytes, PPLP4 expression is specific to the female gender, while ookinetes show a PPLP4 localization at the apical pole. Gene disruption of pplp4 results in no phenotypical change during blood stage replication, gametocyte development or gametogenesis, while mosquitoes fed with PPLP4-deficient gametocytes display a severe reduction in oocyst numbers, and an accumulation of ookinetes in the mosquito midguts was observed. In conclusion, we propose an essential role for PPLP4 in infection of the mosquito midgut, presumably by mediating ookinete traversal through the midgut epithelium.
Asunto(s)
Culicidae/parasitología , Plasmodium falciparum/crecimiento & desarrollo , Proteínas Citotóxicas Formadoras de Poros/metabolismo , Proteínas Protozoarias/metabolismo , Animales , Tracto Gastrointestinal/parasitología , Proteínas Citotóxicas Formadoras de Poros/genética , Proteínas Protozoarias/genéticaRESUMEN
Cyclin-dependent kinase-like kinases (CLKs) are dual specificity protein kinases that phosphorylate Serine/Arginine-rich (SR) proteins involved in pre-mRNA processing. Four CLKs, termed PfCLK-1-4, can be identified in the human malaria parasite Plasmodium falciparum, which show homology with the yeast SR protein kinase Sky1p. The four PfCLKs are present in the nucleus and cytoplasm of the asexual blood stages and of gametocytes, sexual precursor cells crucial for malaria parasite transmission from humans to mosquitoes. We identified three plasmodial SR proteins, PfSRSF12, PfSFRS4 and PfSF-1, which are predominantly present in the nucleus of blood stage trophozoites, PfSRSF12 and PfSF-1 are further detectable in the nucleus of gametocytes. We found that recombinantly expressed SR proteins comprising the Arginine/Serine (RS)-rich domains were phosphorylated by the four PfCLKs in in vitro kinase assays, while a recombinant PfSF-1 peptide lacking the RS-rich domain was not phosphorylated. Since it was hitherto not possible to knock-out the pfclk genes by conventional gene disruption, we aimed at chemical knock-outs for phenotype analysis. We identified five human CLK inhibitors, belonging to the oxo-ß-carbolines and aminopyrimidines, as well as the antiseptic chlorhexidine as PfCLK-targeting compounds. The six inhibitors block P. falciparum blood stage replication in the low micromolar to nanomolar range by preventing the trophozoite-to-schizont transformation. In addition, the inhibitors impair gametocyte maturation and gametogenesis in in vitro assays. The combined data show that the four PfCLKs are involved in phosphorylation of SR proteins with essential functions for the blood and sexual stages of the malaria parasite, thus pointing to the kinases as promising targets for antimalarial and transmission blocking drugs.
Asunto(s)
Antimaláricos/farmacología , Malaria Falciparum/prevención & control , Plasmodium falciparum/efectos de los fármacos , Inhibidores de Proteínas Quinasas/farmacología , Proteínas Serina-Treonina Quinasas/antagonistas & inhibidores , Proteínas Tirosina Quinasas/antagonistas & inhibidores , Proteínas Protozoarias/antagonistas & inhibidores , Animales , Regulación del Desarrollo de la Expresión Génica , Genes Protozoarios , Humanos , Malaria Falciparum/parasitología , Malaria Falciparum/transmisión , Fosforilación , Plasmodium falciparum/genética , Plasmodium falciparum/fisiología , Proteínas Serina-Treonina Quinasas/química , Proteínas Serina-Treonina Quinasas/genética , Proteínas Tirosina Quinasas/química , Proteínas Tirosina Quinasas/genética , Proteínas Protozoarias/química , Proteínas Protozoarias/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/genética , Homología Estructural de ProteínaRESUMEN
Egress of malaria parasites from the host cell requires the concerted rupture of its enveloping membranes. Hence, we investigated the role of the plasmodial perforin-like protein PPLP2 in the egress of Plasmodium falciparum from erythrocytes. PPLP2 is expressed in blood stage schizonts and mature gametocytes. The protein localizes in vesicular structures, which in activated gametocytes discharge PPLP2 in a calcium-dependent manner. PPLP2 comprises a MACPF domain and recombinant PPLP2 has haemolytic activities towards erythrocytes. PPLP2-deficient [PPLP2(-)] merozoites show normal egress dynamics during the erythrocytic replication cycle, but activated PPLP2(-) gametocytes were unable to leave erythrocytes and stayed trapped within these cells. While the parasitophorous vacuole membrane ruptured normally, the activated PPLP2(-) gametocytes were unable to permeabilize the erythrocyte membrane and to release the erythrocyte cytoplasm. In consequence, transmission of PPLP2(-) parasites to the Anopheles vector was reduced. Pore-forming equinatoxin II rescued both PPLP2(-) gametocyte exflagellation and parasite transmission. The pore sealant Tetronic 90R4, on the other hand, caused trapping of activated wild-type gametocytes within the enveloping erythrocytes, thus mimicking the PPLP2(-) loss-of-function phenotype. We propose that the haemolytic activity of PPLP2 is essential for gametocyte egress due to permeabilization of the erythrocyte membrane and depletion of the erythrocyte cytoplasm.
Asunto(s)
Permeabilidad de la Membrana Celular , Membrana Celular/fisiología , Eritrocitos/fisiología , Eritrocitos/parasitología , Perforina/metabolismo , Plasmodium falciparum/fisiología , Proteínas Protozoarias/metabolismo , Técnicas de Inactivación de Genes , Plasmodium falciparum/genética , Proteínas Protozoarias/genéticaRESUMEN
Successful gametogenesis of the malaria parasite depends on egress of the gametocytes from the erythrocytes within which they developed. Egress entails rupture of both the parasitophorous vacuole membrane and the erythrocyte plasma membrane, and precedes the formation of the motile flagellated male gametes in a process called exflagellation. We show here that egress of the male gametocyte depends on the function of a perforin-like protein, PPLP2. A mutant of Plasmodium berghei lacking PPLP2 displayed abnormal exflagellation; instead of each male gametocyte forming eight flagellated gametes, it produced gametocytes with only one, shared thicker flagellum. Using immunofluorescence and transmission electron microscopy analysis, and phenotype rescue with saponin or a pore-forming toxin, we conclude that rupture of the erythrocyte membrane is blocked in the mutant. The parasitophorous vacuole membrane, on the other hand, is ruptured normally. Some mutant parasites are still able to develop in the mosquito, possibly because the vigorous motility of the flagellated gametes eventually leads to escape from the persisting erythrocyte membrane. This is the first example of a perforin-like protein in Plasmodium parasites having a role in egress from the host cell and the first parasite protein shown to be specifically required for erythrocyte membrane disruption during egress.
Asunto(s)
Membrana Eritrocítica/parasitología , Células Germinativas/metabolismo , Perforina/metabolismo , Plasmodium berghei/metabolismo , Proteínas Protozoarias/metabolismo , Animales , Eritrocitos/parasitología , Masculino , Ratones , Ratones Endogámicos , Modelos Animales , Fenotipo , Plasmodium berghei/efectos de los fármacos , Saponinas/farmacología , Motilidad Espermática/fisiología , Cola del Espermatozoide/efectos de los fármacos , Cola del Espermatozoide/fisiología , Cola del Espermatozoide/ultraestructuraRESUMEN
Egress is a crucial step for malaria parasites to progress from one host cell to another. The rapid transition between host cells is mediated by the invasive merozoite stages. Merozoite egress from the enveloping cell includes the rupture of two membranes, the membrane of the parasitophorous vacuole and the host cell membrane. Egress from the host cell is also of importance for the intraerythrocytic gametocytes in order to undergo gametogenesis following their transmission to the mosquito during the blood meal. An increasing number of studies have aimed to identify the molecules involved in host cell egress by malaria parasites and decipher the sequence of membrane rupture. Recent work has acknowledged the crucial roles of plasmodial and host-derived proteases in membrane rupture and has indicated the involvement of secretory vesicles in priming the enveloping membranes for egress. This review highlights recent insight into the mechanisms of host cell egress by Plasmodium parasites. We will discuss the mode of egress of intrahepatic and intraerythrocytic parasites and their measures to evade the host immune system during this process.