1.
J Virol Methods
; 325: 114888, 2024 Apr.
Artículo
en Inglés
| MEDLINE
| ID: mdl-38246565
RESUMEN
We present an amplicon-based assay for MinION Nanopore sequencing of mpox virus (MPXV) genomes from clinical specimens, obtaining high-quality results with an average genome coverage of 99% for Ct values of up to 25, and a genome coverage of 97.1% for Ct values from 25 to 30 which are challenging to sequence. This assay is easy to implement in PCR-based workflows and provides accurate genomic data within a short time.