Topography of the lingual foramen using micro-computed tomography for improving safety during implant placement of anterior mandibular region.

The aim of this study was to determine the location and size of the lingual foramina and the course of their canals using micro-computed tomography. Twenty Korean mandibles were scanned using a micro-computed tomography system and reconstructed three-dimensionally to enable observation of the lingual foramina and their canals. Four mandibles (20%) had a single foramen at the lingual side of the mandibular midline, 8 mandibles (40%) had 2 foramina, and 5 mandibles (25%) had 3 foramina. Three mandibles (15%) had 4 small foramina with short canals. The foramina were classified as either superior lingual foramina or inferior lingual foramina according to their vertical location relative to the mental spine. The diameters of superior lingual foramina and inferior lingual foramina were 0.75 ± 0.36 and 0.73 ± 0.38 mm (mean ± SD), respectively. The distances from the inferior border of the mandible to superior lingual foramina and inferior lingual foramina were 12.58 ± 2.49 and 6.43 ± 3.08 mm, respectively. Ten canals (21%) traveled upward to the labial side, 24 canals (51%) downward, and 13 canals (28%) parallel to it. Ten mandibles (50%) had lateral foramina.With regard to implant surgery of the anterior mandibular region, the most hazardous zones for lingual foramina are 0 to 2 mm from the midline, the upper 3 to 17 mm from the inferior border of the mandible, and the anterior 0 to 7 mm from the lingual side. Careful preoperative planning taking into account the anatomical location of these foramina might help to avoid complications due to damage to the foramina, their canals, and their contents.

Beneficial immunostimulatory effect of short-term Chlorella supplementation: enhancement of natural killer cell activity and early inflammatory response (randomized, double-blinded, placebo-controlled trial).

BACKGROUND: In vitro and animal studies have demonstrated that Chlorella is a potent biological response modifier on immunity. However, there were no direct evidences for the effect of Chlorella supplementation on immune/inflammation response in healthy humans. METHODS: This study was designed for an 8-week randomized, double-blinded, placebo-controlled trial: 5g of Chlorella (n=23) or Placebo (n=28) as form of tablets. Mainly, cytotoxic activities of Natural killer (NK) cells and serum concentrations of interferon-γ, interleukin-1ß and interleukin-12 were measured. RESULTS: After the 8-week, serum concentrations of interferon-γ (p<0.05) and interleukin-1ß (p<0.001) significantly increased and that of interleukin-12 (p<0.1) tended to increase in the Chlorella group. The increments of these cytokines after the intervention were significantly bigger in the Chlorella group than those in the placebo group. In addition, NK cell activities (%) were significantly increased in Chlorella group, but not in Placebo group. The increments of NK cell activities (%) were also significantly bigger in the Chlorella group than the placebo group. Additionally, changed levels of NK cell activity were positively correlated with those of serum interleukin-1ß (r=0.280, p=0.047) and interferon-γ (r=0.271, p<0.005). Signficantly positive correlations were also observed among the changed levels of serum cytokines; between interferon-γ and interleukin-1ß (r=0.448, p<0.001), between interleukin-12 and interleukin-1ß (r=0.416, p=0.003) and between interleukin-12 and interferon-γ (r=0.570, p<001). CONCLUSION: These results may suggest a beneficial immunostimulatory effect of short-term Chlorella supplementation which enhances the NK cell activity and produces interferon-γ and interleukin-12 as well as interleukin-1ß, the Th-1 cell-induced cytokines in healthy people.

Course and distribution of the lingual nerve in the ventral tongue region: anatomical considerations for frenectomy.

The aim of this study was to elucidate the sublingual and intralingual courses of the lingual nerve (LN) in the ventral tongue region, providing a clinical guide for safe surgical procedures such as frenectomy. We evaluated 16 specimens (32 sides) by gross observation after detailed dissections, and a further 6 specimens were examined after Sihler staining. All specimens were harvested from embalmed Korean cadavers. We classified the innervation patterns of the LN into 5 types and confirmed the distribution of the LN in the tip of the tongue. The classification of the LN was made with reference to a line formed by the interlacing of the styloglossus and genioglossus muscles. Based on the course of LN and the presence of a tiny twig (twigs directly innervating the ventral mucosa of the tongue, TM) directly innervating the sublingual mucosa, the course of the LN was classified as being straight, curved, or vertical and with or without the TM. Straight, curved, and vertical courses without the TM were seen in 9.4%, 46.9%, and 18.8% of the cases, respectively. Straight and curved courses with the TM were observed in 6.3% and 18.8% of the cases, respectively. Sihler staining revealed that the tongue tip is innervated by the LN. These findings indicate that surgical manipulations at the ventral tongue region might damage the LN and result in numbness of the tongue tip, and provide a useful anatomic reference for various surgical procedures involving the ventral tongue region.