Transient use of hemolymph for hydraulic wing expansion in cicadas.

Insect wings must be flexible, light, and strong to allow dynamic behaviors such as flying, mating, and feeding. When winged insects eclose into adults, their wings unfold, actuated hydraulically by hemolymph. Flowing hemolymph in the wing is necessary for functioning and healthy wings, both as the wing forms and as an adult. Because this process recruits the circulatory system, we asked, how much hemolymph is pumped into wings, and what happens to the hemolymph afterwards? Using Brood X cicadas (Magicicada septendecim), we collected 200 cicada nymphs, observing wing transformation over 2 h. Using dissection, weighing, and imaging of wings at set time intervals, we found that within 40 min after emergence, wing pads morphed into adult wings and total wing mass increased to ~ 16% of body mass. Thus, a significant amount of hemolymph is diverted from body to wings to effectuate expansion. After full expansion, in the ~ 80 min after, the mass of the wings decreased precipitously. In fact, the final adult wing is lighter than the initial folded wing pad, a surprising result. These results demonstrate that cicadas not only pump hemolymph into the wings, they then pump it out, producing a strong yet lightweight wing.

Mallard landing behavior on water follows a -constant braking strategy.

Many flying animals use optic flow to control their flight. During landing maneuvers, pigeons, hummingbirds, bats, Draco lizards and bees use the -constant braking strategy. This strategy regulates the approach by keeping the ratio of distance to an object and the rate of change of that distance constant. In keeping this ratio, , constant, a variety of deceleration profiles can lead to different collision avoidance behaviors. The landing behaviors listed above all qualify as controlled collisions, where the animal is decelerating into the object. We examined whether the same regulatory strategy is employed by mallards when landing on water. Video of mallard landing behavior was recorded at a local pond and digitized. Kinematic and τ parameters were calculated for each landing (N=177). The Pearson correlation coefficient for τ with respect to time to land was 0.99±0.02, indicating mallards employ a controlled-collision strategy. This result implies regulation by the birds to fix as constant while landing (on average, 0.90±0.13). In comparison with other active flyers, mallards use a higher value of when landing (0.775±0.109, 0.710±0.132 and 0.702±0.052 for pigeons, hummingbirds and bats, respectively). This higher may reflect physical differences in substrate from solid to liquid. The higher compliance of water in comparison to a solid substrate may reduce impact forces that could be injurious on a solid substrate, thereby enabling mallards to approach faster and expend less energy for costly, slow flight.