Asunto(s)
Células Madre Embrionarias/citología , Células Madre Embrionarias/trasplante , Terapia por Láser/métodos , Ratones Transgénicos/genética , Ratones Transgénicos/cirugía , Microinyecciones/métodos , Microcirugia/métodos , Trasplante de Células Madre/métodos , Animales , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C57BL , Ratones Mutantes , Ratones Transgénicos/anatomía & histologíaRESUMEN
Massively parallel signature sequencing (MPSS) is one of the newest tools available for conducting in-depth expression profiling. MPSS is an open-ended platform that analyses the level of expression of virtually all genes in a sample by counting the number of individual mRNA molecules produced from each gene. There is no requirement that genes be identified and characterised prior to conducting an experiment. MPSS has a routine sensitivity at a level of a few molecules of mRNA per cell, and the datasets are in a digital format that simplifies the management and analysis of the data. Therefore, of the various microarray and non-microarray technologies currently available, MPSS provides many advantages for generating the type of complete datasets that will help to facilitate hypothesis-driven experiments in the era of digital biology.
