Synthesis of Spiro Polycyclic N-Heterocycles and Indolecarbazoles via Merging Oxidative Coupling and Cascade Palladium-Catalyzed Intramolecular Oxidative Cyclization.

We report a step-economic strategy for the direct synthesis of spiro polycyclic N-heterocycles and indolecarbazole-fused naphthoquinones by merging oxidative coupling and cascade palladium-catalyzed intramolecular oxidative cyclization. In the protocol, bi-indolylnaphthoquinones were first synthesized by oxidative coupling of indoles and naphthoquinones. Subsequent cascade palladium-catalyzed intramolecular oxidative cyclization of bi-indolylnaphthoquinones gave spiro polycyclic N-heterocycles and indolecarbazoles. The intramolecular oxidative cyclization approach could also be realized by the presence or absence of iron catalysts under standard conditions. This protocol is featured with moderate to excellent yields, a wide substrate scope, and divergent structures of products.

Prevalence of depressive tendencies among college students and the influence of attributional styles on depressive tendencies in the post-pandemic era.

Introduction: Depression symptoms are prevalent globally, including China, with a notable impact on college students. This study aims to not only estimate the prevalence of depressive tendencies and attributional styles among college students in the post-pandemic era but also explore the relationship between the two factors. The findings of this study can provide new insights into early intervention and support services for individuals exhibiting tendencies toward depression. Methods: The survey was administered to college students from various academic backgrounds at a specific university in southern Jiangxi Province by employing two scales to investigate depressive tendencies and attributional styles. Depressive tendency was evaluated using the Patient Health Questionnaire-9 (PHQ-9), while the attributional styles were assessed using the Multidimensional-Multiattributional Causality Scale (MMCS). Univariate analysis and multiple logistic regressions were conducted to identify the related factors of depressive tendency. Results: A relatively high (48.9%) prevalence of depression among college students was found in this study. College students with higher grades (OR = 1.574, 95%CI: 1.369-1.810), profession of medicine and allied health sciences (OR = 1.779, 95%CI: 1.203-2.629), experiencing higher study stress (OR = 2.006, 95%CI: 1.601-2.514), and having poor physical condition (OR = 1.527, 95%CI: 1.247-1.869) were identified as risk factors for depressive tendency. The correlation between higher grades and increased learning pressure, coupled with poorer physical condition, heightens the vulnerability of college students to depression. Moreover, the more they attribute these experiences to achievement effort (OR = 0.897, 95%CI: 0.828-0.972), achievement ability (OR = 0.903, 95%CI: 0.838-0.972), and affiliation context (OR = 0.919, 95%CI: 0.860-0.982), the less likely they are to develop depression. Conclusion: In the group of college students, especially those in higher grades, profession of medicine and allied health sciences or experiencing high learning pressure and poor physical condition, emphasizing the significance of their mental well-being becomes crucial. Offering suitable support and assistance is essential. Additionally, fostering the cultivation of positive attributional and coping strategies by attributing difficulties to controllable factors and instilling a belief in their ability to overcome challenges can help reduce the risk of depression.

Factors Associated with Intention to Use Telerehabilitation for Children with Special Needs: A Cross-Sectional Study.

Background: Children with special health care needs (CSHCN) require long-term and ongoing rehabilitation interventions supporting their development. Telerehabilitation can provide continuous rehabilitation services for CSHCN. However, few studies have explored the intention of CSHCN and their caregivers to use telerehabilitation and its impact on them. Objective: The objective of this study was to identify factors that influence the intention to use telerehabilitation among CSHCN and their caregivers. Methods: This study was a cross-sectional study. Based on the unified theory of acceptance and use of technology, extended with additional predictors (trust and perceived risk [PR]), this study developed a research model and proposed 10 hypotheses. A structured questionnaire was distributed to 176 caregivers. Data were analyzed and research hypotheses were tested using partial least squares structural equation modeling to better understand the factors influencing the use of telerehabilitation. Results: A total of 164 valid questionnaires were collected. CSHCN and their caregivers were overall satisfied with this telerehabilitation medical service. The results of the structural model analysis indicated that social influence (SI), facilitating conditions (FC), and trust had significant effects on behavioral intention (BI) to use telerehabilitation, while the paths between performance expectancy (PE), effort expectancy (EE), and PR and BI were not significant. PE, EE, and SI had a significant effect on trust. Moreover, EE and SI had indirect effects on BI, with trust as the mediator. Conclusions: The results indicated that SI, FC, and trust are significant factors influencing CSHCN and their caregivers' use of telerehabilitation. Trust is also an important mediator for the intention and highly influenced by PE, EE, and SI.

DZ2002 alleviates corneal angiogenesis and inflammation in rodent models of dry eye disease via regulating STAT3-PI3K-Akt-NF-κB pathway.

Dry eye disease (DED) is a prevalent ocular disorder with a multifactorial etiology. The pre-angiogenic and pre-inflammatory milieu of the ocular surface plays a critical role in its pathogenesis. DZ2002 is a reversible type III S-adenosyl-L-homocysteine hydrolase (SAHH) inhibitor, which has shown excellent anti-inflammatory and immunosuppressive activities in vivo and in vitro. In this study, we evaluated the therapeutic potential of DZ2002 in rodent models of DED. SCOP-induced dry eye models were established in female rats and mice, while BAC-induced dry eye model was established in female rats. DZ2002 was administered as eye drops (0.25%, 1%) four times daily (20 µL per eye) for 7 or 14 consecutive days. We showed that topical application of DZ2002 concentration-dependently reduced corneal neovascularization and corneal opacity, as well as alleviated conjunctival irritation in both DED models. Furthermore, we observed that DZ2002 treatment decreased the expression of genes associated with angiogenesis and the levels of inflammation in the cornea and conjunctiva. Moreover, DZ2002 treatment in the BAC-induced DED model abolished the activation of the STAT3-PI3K-Akt-NF-κB pathways in corneal tissues. We also found that DZ2002 significantly inhibited the proliferation, migration, and tube formation of human umbilical endothelial cells (HUVECs) while downregulating the activation of the STAT3-PI3K-Akt-NF-κB pathway. These results suggest that DZ2002 exerts a therapeutic effect on corneal angiogenesis in DED, potentially by preventing the upregulation of the STAT3-PI3K-Akt-NF-κB pathways. Collectively, DZ2002 is a promising candidate for ophthalmic therapy, particularly in treating DED.

Understanding the use intention and influencing factors of telerehabilitation in people with rehabilitation needs: a cross-sectional survey.

Objective: This study aimed to investigate the use intention and influencing factors of telerehabilitation in people with rehabilitation needs. Methods: This cross-sectional survey recruited a total of 183 participants with rehabilitation needs from May 2022 to December 2022. Sociodemographic and medical data were collected by a structured questionnaire. The factors influencing the use intention of telerehabilitation were measured by the extended Unified Theory of Acceptance and Use of Technology (UTAUT) model. Multiple hierarchical regression analyses were performed. Results: A total of 150 valid questionnaires were included for analysis. The results indicated that the use intention of telerehabilitation was overall high in people with rehabilitation needs. Health condition (ß = -0.21, p = 0.03), performance expectancy (ß = 0.21, p = 0.01), facilitating conditions (ß = 0.25, p = 0.03), perceived trust (ß = 0.25, p < 0.01), and self-efficacy (ß = 0.19, p = 0.04) were significant factors influencing the use intention of telerehabilitation. Conclusion: Overall, the use intention of telerehabilitation is high in individuals with rehabilitation needs. Health conditions, performance expectancy, facilitating conditions, perceived trust, and self-efficacy are important factors influencing the use intention of telerehabilitation in individuals with rehabilitation needs.

Determination of the surface properties and adsorption states of nanoporous materials using the zeta adsorption isotherm.

The adsorption process of porous materials has always been a popular field of research in interfacial physics, and the surface physical parameters of materials can be obtained from their adsorption characteristics, which has a great influence on the performance of materials. Based on the zeta adsorption isotherm, we propose a method based on the zeta adsorption isotherm to predict the entire adsorption process of porous materials and determine material surface properties from the measured isotherm data in the heterogeneity-free range. We applied the zeta constants of the silica adsorption system to the corresponding adsorption isotherm of the porous material. The results showed that the predicted adsorption isotherms are in good agreement with the experimental measurements before pore filling and can effectively identify the pressure ratios at the beginning and end of pore filling. In the region of high-pressure ratios, the Kelvin equation was utilized to calculate the pressure ratio at a contact angle of 0°. The surface parameters of the materials were determined by geometrically calculating the variation of the adsorption amount and the desorption isotherms in the high-pressure ratio range were calculated from these surface parameters. The predicted desorption isotherms can well reflect the adsorption process of silica porous materials in the region of a high-pressure ratio. In addition, for the surface parameters of the materials, the specific surface area calculated from the adsorption and desorption isotherms, respectively, differed by less than 7.9%, and the reliability of the method was verified by comparing the results with those of the argon adsorption systems.

GmJAZ3 interacts with GmRR18a and GmMYC2a to regulate seed traits in soybean.

Seed weight is usually associated with seed size and is one of the important agronomic traits that determine yield. Understanding of seed weight control is limited, especially in soybean plants. Here we show that Glycine max JASMONATE-ZIM DOMAIN 3 (GmJAZ3), a gene identified through gene co-expression network analysis, regulates seed-related traits in soybean. Overexpression of GmJAZ3 promotes seed size/weight and other organ sizes in stable transgenic soybean plants likely by increasing cell proliferation. GmJAZ3 interacted with both G. max RESPONSE REGULATOR 18a (GmRR18a) and GmMYC2a to inhibit their transcriptional activation of cytokinin oxidase gene G. max CYTOKININ OXIDASE 3-4 (GmCKX3-4), which usually affects seed traits. Meanwhile, the GmRR18a binds to the promoter of GmMYC2a and activates GmMYC2a gene expression. In GmJAZ3-overexpressing soybean seeds, the protein contents were increased while the fatty acid contents were reduced compared to those in the control seeds, indicating that the GmJAZ3 affects seed size/weight and compositions. Natural variation in JAZ3 promoter region was further analyzed and Hap3 promoter correlates with higher promoter activity, higher gene expression and higher seed weight. The Hap3 promoter may be selected and fixed during soybean domestication. JAZ3 orthologs from other plants/crops may also control seed size and weight. Taken together, our study reveals a novel molecular module GmJAZ3-GmRR18a/GmMYC2a-GmCKXs for seed size and weight control, providing promising targets during soybean molecular breeding for better seed traits.

Atomistic investigation on the kinetic behavior of vapour adsorption and cluster evolution using a statistical rate theory approach.

The kinetic behavior of vapor adsorption on a solid surface in an isobaric-isothermal system is investigated by means of molecular dynamics simulations combined with theoretical studies through a statistical rate theory approach. The molecular insights into the formation and evolution of clusters in the adsorbate are presented. Results show that the argon vapor is adsorbed on the silicon surface as different types of clusters. In the initial stage of adsorption, the empty adsorption sites on the surface decrease, and the adsorbed single-molecule-cluster grows rapidly and dominates the interface. The increasing rate of the adsorbed cluster and the declining rate of the empty adsorption site are dependent on the pressure ratio. For a large pressure ratio, the single-molecule-clusters are aggregated to incubate large clusters, and the fraction of a single-molecule-cluster is decreased with time. When the adsorption isotherm is determined, the chemical potential of the adsorbed cluster is expressed from the zeta isotherm model. Then the adsorption kinetics are analyzed through the statistical rate theory. The molecular exchange rate and the instantaneous driving force are calculated. The higher pressure ratio induces the larger chemical potential difference and accelerates the net adsorption rate. The adsorption kinetics derived from MD simulations are in close agreement with the theoretical analysis of the statistical rate theory.

Study on Evaporation Characteristics of Water in Annular Liquid Pool at Low Pressures.

In order to investigate the energy transfer mechanism and the nonequilibrium effect during water evaporation in its own pure vapor at low pressures, a series of precise measurements are conducted to obtain the temperature profile near the liquid-vapor interface and the evaporation rates in an annular pool in a closed chamber. The results show that the interface temperature of the vapor side is higher than that of the liquid side when water evaporates in its own pure vapor at low pressures (ranging from 394 to 1467 Pa), the temperature discontinuity across the interface exists in all experimental conditions. The magnitude of the temperature discontinuity is strongly affected by the vapor pressure. A uniform temperature layer with a thickness of about 2 mm is found below the evaporating interface because of the coupling effect of evaporation cooling and thermocapillary convection. The energy required for evaporation is mainly transferred by thermocapillary convection in the uniform temperature layer. Furthermore, the numerical simulation results confirm that the evaporation flux near the cylinders is much larger than that at the middle region, which implies that most of the latent heat required for evaporation is transferred to the interface near the cylinders.

Molecular insight into the formation of adsorption clusters based on the zeta isotherm.

This work presents a series of molecular dynamics simulations of argon adsorption on a silicon substrate with different lattice orientations. From the simulation results, the density profiles are discussed and the amount of adsorbed particles is obtained at different pressures. It is found that the solid surface orientation has a great influence on the density distributions and atomic arrangements near the surface. With the collected data, the thermal constants derived from the expression of zeta adsorption isotherms are determined. The calculated isotherms agree well with the simulation results. Also, from a microscopic point of view, the molecular insights show that the structures of the adsorbates are present as clusters with different numbers of particles. The size of the clusters changes with pressure. At a relatively small pressure ratio, most of the clusters consist of a single molecule. As the pressure ratio increases, larger sized clusters appear, forming various cluster-types. The molecular cluster distributions are closely consistent with the basic approximation of the zeta adsorption isotherm. Furthermore, the surface adsorption sites determined from molecular dynamics simulation show good agreement with that predicted by the zeta isotherm model, which reaffirms the effectiveness of the theoretical model. When the isotherm is extended to a pressure ratio greater than unity, a finite amount of adsorption is predicted and the wetting conditions are obtained. Affected by the solid surface orientations, the pressure ratio at wetting for the silicon substrate with the (111) surface plane is larger than those of the (100) and (110) surfaces, indicating that a higher subcooling is required for the wetting transition.

Molecular Dynamics Simulation of Heat Transport through Solid-Liquid Interface during Argon Droplet Evaporation on Heated Substrates.

This paper presents a series of molecular dynamics simulations of the evaporating process of an argon droplet on heated substrates and the energy transport mechanism through the solid-liquid interface. Results indicate that the mass density through the liquid-vapor interface decreases sharply when the evaporation is in the steady state. Meanwhile, there is an adsorption layer in the form of clusters at the solid-liquid interface, which has a higher mass density than the droplet inside. Furthermore, the wetting property of the solid substrate is related to the system's initial temperature and the solid-liquid potential energy parameter. The contact angle decreases with the increase of initial temperature and solid-liquid potential energy parameter. During the accelerated evaporation process, small part of energy transports into the liquid in the perpendicular direction to the solid-liquid interface and most of the energy transports along the parallel direction to the solid-liquid interface in the adsorption layer to the three-phase contact line. The heat-transfer process from the solid substrate to the droplet inside is hindered by the Kapitza resistance at the solid-liquid interface, no matter the solid substrate is hydrophilic or hydrophobic. Meanwhile, the Kapitza resistance gradually increases with the increase of the initial temperature and decreases with the increase of the solid-liquid energy parameter.

Helicobacter pylori with East Asian-type cagPAI genes is more virulent than strains with Western-type in some cagPAI genes

Abstract The severity of Helicobacter pylori-related disease is correlated with the presence and integrity of a cag pathogenicity island (cagPAI). cagPAI genotype may have a modifying effect on the pathogenic potential of the infecting strain. After analyzing the sequences of cagPAI genes, some strains with the East Asian-type cagPAI genes were selected for further analysis to examine the association between the diversity of the cagPAI genes and the virulence of H. pylori. The results showed that gastric mucosal inflammatory cell infiltration was significantly higher in patients with East Asian-type cagPAI genes H. pylori strain compared with mosaicism cagPAI genes H. pylori strain (p < 0.05). H. pylori strains with the East Asian-type cagPAI genes were closely associated with IL-8 secretion in vitro and in vivo compared with H. pylori strains with the mosaicism cagPAI genes (p < 0.01). H. pylori strains with East Asian-type cagPAI genes are able to strongly translocate CagA to host cells. These results suggest that H. pylori strains with East Asian-type cagPAI genes are more virulent than the strains of cagPAI gene/genes that are Western type.

Helicobacter pylori with East Asian-type cagPAI genes is more virulent than strains with Western-type in some cagPAI genes.

The severity of Helicobacter pylori-related disease is correlated with the presence and integrity of a cag pathogenicity island (cagPAI). cagPAI genotype may have a modifying effect on the pathogenic potential of the infecting strain. After analyzing the sequences of cagPAI genes, some strains with the East Asian-type cagPAI genes were selected for further analysis to examine the association between the diversity of the cagPAI genes and the virulence of H. pylori. The results showed that gastric mucosal inflammatory cell infiltration was significantly higher in patients with East Asian-type cagPAI genes H. pylori strain compared with mosaicism cagPAI genes H. pylori strain (p<0.05). H. pylori strains with the East Asian-type cagPAI genes were closely associated with IL-8 secretion in vitro and in vivo compared with H. pylori strains with the mosaicism cagPAI genes (p<0.01). H. pylori strains with East Asian-type cagPAI genes are able to strongly translocate CagA to host cells. These results suggest that H. pylori strains with East Asian-type cagPAI genes are more virulent than the strains of cagPAI gene/genes that are Western type.

Helicobacter pylori with the Intact dupA Cluster is more Virulent than the Strains with the Incomplete dupA Cluster.

The duodenal ulcer promoting gene (dupA), located in the plasticity region of Helicobacter pylori (H. pylori), is predicted to form a type IV secretory system (T4SS) with vir genes around dupA. In the study, we investigated the association between the dupA cluster status and the virulence of H. pylori in a littoral region of Northeast China. Two hundred and sixty-two H. pylori strains isolated from the chronic gastritis were examined to evaluate the dupA cluster status, cag PAI genes and vacA genotype using PCR and Western blot. Histopathologic evaluations of biopsy specimens were performed to analysis the association between the dupA cluster and the inflammatory response. IL-8 productions in gastric mucosa and from GES-1 cells co-cultured with H. pylori were measured, respectively, to analysis the association between the dupA cluster status and IL-8 production. We found that gastric mucosal inflammatory cell infiltration was significantly higher in patients with dupA-positive H. pylori, including H. pylori with complete dupA cluster (2.71 ± 0.79) and incomplete dupA cluster (2.09 ± 0.61) than in patients with dupA-negative strain (1.73 ± 0.60, p < 0.01), whereas no significant difference in the gastric mucosal atrophy was found according to the status of dupA cluster. Gastric mucosal IL-8 levels were higher in the complete dupA cluster group than in other groups (p < 0.01), and IL-8 production from GES-1 cells was also significantly higher in strains with a complete dupA cluster (1527.9 ± 180.0 pg/ml) than in those with an incomplete dupA cluster (1229.4 ± 75.3 pg/ml, p < 0.01) or those with dupA negative (1201.9 ± 92.3 pg/ml, p < 0.01). In conclusion, the complete dupA cluster in H. pylori is associated with inflammatory cell infiltration and IL-8 secretion, and H. pylori strain with a complete dupA cluster seems to be more virulent than other strains with the incomplete dupA cluster or dupA negative.

[Analysis of road traffic injuries from Chinese National Injury Surveillance System, 2006 - 2008].

OBJECTIVE: To describe the distribution of road traffic injuries through hospital based National Injury Surveillance System(NISS). METHODS: Data of road traffic injuries was descriptively analyzed from Chinese NISS from 2006 to 2008. RESULTS: In 2006 - 2008, road traffic injury was the second leading cause from NISS among attendants in ERs or clinics of the hospitals, with males (64.63%, 64.07%, 64.38%) more than females (35.37%, 35.93%, 35.62%). People aged 30 - 44 (36.04%, 34.82%, 34.28%), 15 - 29 (30.74%, 31.57%, 30.13%), 45 - 64 (20.28%, 20.70%, 22.80%) years were seen more than other age groups. The majority of road traffic injuries were unintentional (98.34%, 99.07%, 99.07%), and mostly injured in head (35.21%, 33.74%, 35.77%) and lower limbs (24.08%, 24.54%, 23.95%) which mainly as bruise (56.47%, 57.92%, 58.89%) and fractures (17.70%, 15.84%, 15.88%). The severities of injuries were mainly minor ones (63.69%, 67.24%, 65.68%), and mostly went home right after treatments (59.43%, 63.76%, 62.80%). CONCLUSION: The distribution of road traffic injuries from NISS kept stable from 2006 to 2008. Young and middle aged men were the focus population for road traffic injuries intervention. Further improvement of NISS, multi-sectional collaboration-based advocacies and education programs as well as the enforcement of road safety law seemed the good practices for road traffic injury prevention.

[Analysis of poisoning cases from Chinese National Injury Surveillance System, 2006 - 2008].

OBJECTIVE: To study the preventive strategies through analyzing the poisoning cases from the National Injury Surveillance System (NISS), from 2006 to 2008. METHODS: Data of poisoning cases was descriptively analyzed from Chinese NISS, from 2006 to 2008. RESULTS: The proportion of poisoning cases to all injuries cases from NISS were 2.57%, 2.48% and 2.52% from 2006 to 2008, which ranked sixth in all the injuries causes. Most people being poisoned had junior middle school education and most of them were agriculture/animal husbandry/fishery/water producers or commercial/service personnel. Most of the poisoning incidents were happened at home, always occurred in leisure time - around 8 PM, every day. The common types of poisoning were alcohol, clinical drugs, pesticide and carbon monoxide. Unintentional injuries were the main causes. Self-harm/suicidal cases in the rural areas were more than in the urban areas, with women more than men. The main type of self-harm/suicide related poisoning cases were through drugs or pesticide. ≥ 65, 15 - 29 and 30 - 44 year-olds were most commonly seen. CONCLUSION: Alcoholism was the primary type of poisoning injuries which is the highest in young adults (15 - 29 years and 30 - 44 years). It's important to promote civilized drinking habits and limit access to alcohol for youth. Self-harm/suicide had close relationship with clinical drugs and pesticide. The key points to prevent pesticide and clinical drugs poisoning were safe storage of pesticides, universal security of pesticide, and the supervision on drug producing and marketing. Children and the elderly were the high risk people for carbon monoxide poisoning. Monitoring and intervention must be strengthened.

[Analysis on the characteristics of injuries from the Chinese National Injury Surveillance System].

OBJECTIVE: To analyze the injury characteristics, using data from the National Injury Surveillance System (NISS), 2006 to 2008, to develop a injury prevention strategy. METHODS: 129 hospitals from 43 surveillance points in NISS were selected according to the DSP sample framework, also considering the capacity of local CDC. RESULTS: Relative information on 1 318 739 cases were analyzed. The number of male cases were more than the females. The top three causes of injuries were as follows: falls (28.02%, 29.36%, 30.12%), traffic-related (23.07%, 21.61%, 21.44%) and blunt injuries (21.46%, 21.17%, 20.59%). The major places where injuries occurred were: road/street (29.80%, 27.73%, 28.37%) and at home (21.51%, 22.80%, 24.02%). Recreation activities (35.63%, 41.93%, 42.13%) and at work (24.43%, 22.60%, 21.44%) were the two major activities when injuries took place. Most injury cases were unintentional (83.81%, 86.32%, 86.71%) and mild (70.42%, 74.79%, 73.90%). Rush hours that caused injuries to happen were between 7 to 10 AM. CONCLUSION: Male injury cases were more than females. Falls, road traffic-related and blunt injuries were the major causes, with most of them were unintentional and minor.

[Distribution of children cases in the Chinese National Injury Surveillance System, 2006 - 2008].

OBJECTIVE: To understand the pattern of children injuries treated in hospitals and to provide evidence for its further prevention and control. METHODS: Data of children cases was descriptively analyzed from the Chinese National Injury Surveillance System (NISS), from 2006 to 2008. RESULTS: The incidence rates of boys were twice (2.22, 2.15, 2.15) higher than girls. About one half (50.74%, 49.75%, 49.75%) of them were 5-14 year of age. The main causes were falls (44.14%, 45.68%, 47.15%), RTIs (15.71%, 14.46%, 13.79%), and blunt force injuries (13.20%, 12.92%, 12.40%). Main locations where the injuries happened were at home (34.96%, 36.86%, 38.84%), school and public places (24.72%, 19.80%, 21.19%), and road/street (21.21%, 19.63%, 19.33%). The major injured body parts were head (34.88%, 35.84%, 37.07%), upper limbs (28.00%, 28.21%, 27.81%) and lower limbs (21.86%, 21.49%, 21.31%). The majority cases were unintentional (93.01%, 92.66%, 90.58%), minor (78.50%, 81.20%, 81.52%) injuries, treated and discharged (82.37%, 85.19%, 84.84%). There were considerable differences in the distributions of causes, locations, injured body parts, and other contexts depending on age. CONCLUSION: The pattern of children's injury would include causes, places and other characteristics treated at hospitals from NISS and was related to the age of the injured child which was quite different from the spectrum of death caused by injury. NISS acts as an important source of information on children's injury in China, and can contribute to the prevention and control program on children's injury.

[Analysis on injuries among the elderly from Chinese Injury Surveillance System, 2006 - 2008].

OBJECTIVE: To describe the distribution of injuries among the elderly through hospital based National Injury Surveillance System (NISS), and to provide basic information for prevention of injury among the elderly. METHODS: Data on injuries among the elderly aged 60 years and above was descriptively analyzed from the Chinese NISS from 2006 to 2008. Using the data of NISS from 2006 to 2008, information was collected on those cases who were diagnosed as injuries for the first-time in the site hospitals. Information included demographic characteristics, features of the injury events, and clinical characteristics of injuries. SPSS 16.0 software was used for data analysis. RESULTS: The leading causes of injuries among the elderly were: falls, road traffic injuries, and blunt injuries, which were in total accounting for more than 70% of all the injuries among the elderly. Most of the injuries occurred at home (46.25%, 44.44%, 46.23%), on the roads (30.95%, 28.51%, 28.61%) and in the public residences (8.89%, 11.57%, 11.25%). Recreation activates (42.14%, 48.09%, 48.59%), housework/study (21.15%, 18.21%, 18.15%) and driving (13.67%, 13.80%, 13.56%) were the three major activities when injuries took place. Most injuries among the elderly were unintentional (91.34%, 92.84%, 93.09%). The leading causes of suicide of the elderly were poisoning and sharp injury. The most common natures of injuries were bruise (36.07%, 38.18%, 38.50%), fractures (25.65%, 22.47%, 23.77%), and sharp/bite/open injuries (17.19%, 18.21%, 17.46%) while the most injured part of bodies were head (26.32%, 25.59%, 25.90%), lower limbs (25.74%, 25.70%, 25.26%) and upper limbs (21.01%, 21.14%, 21.37%). Most of the injuries were minor ones (60.53%, 65.09%, 63.58%), and patients mostly went home after treatment (64.11%, 68.53%, 67.99%). CONCLUSION: According to NISS, the proportion of injuries among the elderly in all injuries kept increasing from 2006 to 2008, while distribution of injuries among the elderly being stable. Falls was the most common injury type for the elderly which also brought along bad outcome. Falls was the priority for injury prevention among the elderly.

[Single nucleotide polymorphisms of DAT and DRD(2) genes in Han Chinese population and their association with stuttering].

OBJECTIVE: To explore the correlations of dopamine transporter gene (DAT) and dopamine D(2) receptor gene (DRD2) to stuttering. METHODS: To examine the correlations of the 5 single nucleotide polymorphisms (SNPs) in dopaminergic gene (C252T, C1804T, and C1820T in DAT gene, and T1054C and C1072T in DRD(2) gene) to stuttering in Han Chinese individuals, a case-control study involving 112 patients with stuttering and 112 gender-matched controls was carried out. Genotyping was performed by a combined approach using polymerase chain reaction (PCR) and pyrosequencing. RESULTS: C1804T showed no polymorphism in either the patients or the control subjects and was therefore excluded from the following analysis. The C allele frequency at C1072T site was significantly higher, but T allele frequency significantly lower in the stuttering group than in the control group. The patients had significantly higher CC and lower CT genotype frequencies than the control group. There were no significant differences in the allelic frequencies of C252T, C1820T and T1054C between the patients and the controls, suggesting a Hardy-Weinberg equilibrium at these 3 loci. CONCLUSION: The presence of the C allele at C1072T in DRD(2) gene is associated with increased susceptibility to stuttering in Han Chinese, whereas the T allele provides protection against the onset of stuttering.