Pickering emulsion with high freeze-thaw stability stabilized by xanthan gum/lysozyme nanoparticles and konjac glucomannan.

In this study, freeze-thaw cycle experiments were conducted on food-grade Pickering emulsions co-stabilized with konjac glucomannan (KGM) and xanthan gum/lysozyme nanoparticles (XG/Ly NPs). The rheological properties, particle size, flocculation degree (FD), coalescence degree (CD), centrifugal stability, Differential scanning calorimetry (DSC), X-ray diffraction (XRD) and microstructure of Pickering emulsion stabilized by KGM before and after freeze-thaw were characterized. It was found that as the concentration of KGM increased, the flocculation degree (FD) and coalescence degree (CD) of the emulsion decreased after the freeze-thaw cycle compared to the control sample, and the microscopic images showed that the droplets became smaller and less affected by the freeze-thaw cycles. The rheological and water-holding properties also confirmed that the KGM-added emulsions still had a strong gel network structure and prevented the separation of the continuous and dispersed phases of the droplets after freezing and thawing. Freeze-thaw treatments had a negative effect on the stable emulsion of XG/Ly NPs, while the addition of KGM improved the freeze-thaw stability of the emulsion, which provided a theoretical basis for the development of emulsion products with high freeze-thaw stability.

Rheological and physicochemical properties of heat-induced ovalbumin gels in presence of sodium carboxymethyl cellulose.

In this study, the effect of sodium carboxymethylcellulose (CMC-Na) on the rheological and physicochemical properties of heat-induced ovalbumin (OVA) gels was evaluated. The OVA/CMC-Na composite gels were prepared by heat-induced (85 °C, pH 7.0) a mixture of CMC-Na (0, 0.2, 0.4, 0.8 and 1%) and OVA. The results revealed that the addition of CMC-Na dramatically reduced the springiness and hardness of the composite gels, while slightly enhancing the intermolecular hydrogen bonding interactions, which facilitated the improvement of the softness of the gels. It can be observed by SEM that the added CMC-Na was stacked on the surface of the OVA, resulting in visible "linear bumps". All gel samples exhibited shear-thinning behavior. The apparent viscosity of the composite gels increased with the addition of CMC-Na, and the OVA gel with 1% CMC-Na showed the highest apparent viscosity and the lowest storage modulus (G'). Additionally, low field nuclear magnetic resonance (LF-NMR) measurements indicated that the increasing CMC-Na boosted the water mobility of the composite gel. This study offers a novel approach to the development of ovalbumin-based soft gel foods, especially for certain populations with swallowing difficulties.

Insight into the interaction between tannin acid and bovine serum albumin from a spectroscopic and molecular docking perspective.

In this study, the interaction mechanism of bovine serum albumin (BSA) with tannic acid (TA) was investigated by spectroscopic and computational approaches and further validated using circular dichroism (CD), differential scanning calorimetry (DSC) and molecular docking techniques. The fluorescence spectra showed that TA bound to BSA and underwent static quenching at a single binding site, which was consistent with the molecular docking results. And the fluorescence quenching of BSA by TA was dose-dependent. Thermodynamic analysis indicated that hydrophobic forces dominated the interaction of BSA with TA. The results of circular dichroism showed that the secondary structure of BSA was slightly changed after coupling with TA. Differential scanning calorimetry showed that the interaction between BSA and TA improved the stability of the BSA-TA complex, and the melting temperature increased to 86.67 °C and the enthalpy increased to 264.1 J g-1 when the ratio of TA to BSA was 1.2 : 1. Molecular docking techniques revealed specific amino acid binding sites for the BSA-TA complex with a docking energy of -12.9 kcal mol-1, which means the TA is non-covalently bound to the BSA active site.

Stabilities, Electronic Structures, and Bonding Properties of 20-Electron Transition Metal Complexes (Cp)2TMO and their One-Dimensional Sandwich Molecular Wires (Cp = C5H5, C5(CH3)H4, C5(CH3)5; TM = Cr, Mo, W).

First-principles calculations have been carried out for the 20-electron transition metal complexes (Cp)2TMO and their molecular wires (Cp = C5H5, C5(CH3)H4, C5(CH3)5; TM = Cr, Mo, W). The calculation results at the BP86/def2-TZVPP level reveal that the ground state is singlet and the optimized geometries are in good agreement with the experimental values. The analysis of frontier molecular orbitals shows that two electrons in the highest occupied molecular orbital HOMO-1 are mainly localized on cyclopentadienyl and oxygen ligands. Furthermore, the nature of the TM-O bond was investigated with the energy decomposition analysis-natural orbitals for chemical valence (EDA-NOCV). The attraction term in the intrinsic interaction energies ΔEint is mainly composed of two important parts, including electrostatic interaction (about 52% of the total attractive interactions ΔEelstat + ΔEorb) and orbital interaction, which might be the major determinant of the stability of these (Cp)2TMO complexes. All of the TM-O bonds should be described as electron-sharing σ single bonds [(Cp)2TM]+-[O]- with the contribution of 53-57% of ΔEorb and two π backdonations from the occupied p orbitals of oxygen ligands into vacant π* MOs of the [(Cp)2TM]+ fragments, which are 35-40% of ΔEorb. The results of bond order and interaction energy from EDA-NOCV calculations suggest the influence of the radius of TM and methyl in the interactions between TM and O in (Cp)2TMO. Additionally, the relativistic effects slightly amplify the strength of bonding with increasing ΔEorb for the EDA-NOCV calculations on three metal complexes (C5H5)2TMO. Finally, the geometries, electronic structures, and magnetics of infinitely extended systems, [(C5H5)TMO]∞, have also been explored. The results of the density of states (DOS) and band structure revealed that [(C5H5)CrO]∞ and [(C5H5)WO]∞ are semiconductors with the narrow bands, whereas [(C5H5)MoO]∞ behaves as metal.