Spatial utilization of historical topographic map and its application in land reconstruction of ancient Chinese urban land use.

The historical topographic map preserves rich geographic information and can provide direct assistance for the reconstruction of various geographic elements. Based on the historical data of cities throughout the Qing Dynasty, the land use scale data of cities across the country was obtained using GIS and urban perimeter conversion models. This study combines city information and city circumference records from the historical maps and archives of the late Qing Dynasty to quantitatively reconstruct the use patterns of ancient China's urban land at a spatial resolution of 1° × 1°. Uncertainty analysis of the reconstruction results was conducted using modern remote sensing image data as the validation data set. The results showed the following. (1) During the late Qing Dynasty, the total area of urban land in the various provinces and regions was 1456.015 km2. The maximum value was 208.691 km2 in Beijing-Tianjin-Hebei, the minimum value was 1.713 km2 in Qinghai, and the average value was 56.001 km2. (2) The results of grid reconstruction show that among the 398 grids with urban land distribution, the maximum value is 64.099 km2/grid, the minimum value is 0.013 km2/grid, and the average value is 3.658 km2/grid. (3) Of all the grids with urban land, the urban land grid to the west of the Hu Line accounts for 12.5% and the east to 87.5%. (4) During the late Qing Dynasty, urban land use in China was primarily concentrated in agriculturally developed areas such as the North China Plain, the Central Plains, Jiangnan, and the Sichuan-Chongqing region. (6) The results of a kernel density estimation showed that there were obviously three core areas of urban land agglomeration in China during the late Qing Dynasty: the North China Plain-Central Plains, the Jiangsu-Shanghai-Zhejiang-Anhui area, and the Sichuan-Chongqing urban core area. This study provides basic data for urban land use during historical periods and provides a basis for the quantitative reconstruction of relevant urban land data for historical archives.

Light-Induced TaHY5-7A and TaBBX-3B Physically Interact to Promote PURPLE PERICARP-MYB 1 Expression in Purple-Grained Wheat.

Purple-grained wheat (Triticum aestivum L.) is an important germplasm source in crop breeding. Anthocyanin biosynthesis in the pericarps of purple-grained wheat is largely light-dependent; however, the regulatory mechanisms underlying light-induced anthocyanin accumulation in the wheat pericarp remain unknown. Here we determined that anthocyanins rapidly accumulate in the pericarps of the purple-grained wheat cultivar Heixiaomai 76 (H76) at 16 days after pollination under light treatment. Using transcriptome sequencing, differential gene expression analysis, and phylogenetic analysis, we identified two key genes involved in light signaling in wheat: ELONGATED HYPOCOTYL 5-7A (TaHY5-7A) and B-BOX-3B (TaBBX-3B). TaHY5-7A and TaBBX-3B were highly expressed in purple-grained wheat pericarps. The heterologous expression of TaHY5-7A partially restored the phenotype of the Arabidopsis (Arabidopsis thaliana) hy5 mutant, resulting in increased anthocyanin accumulation and a shortened hypocotyl. The heterologous expression of TaBBX-3B in wild-type Arabidopsis had similar effects. TaHY5-7A and TaBBX-3B were nucleus-localized, consistent with a function in transcription regulation. However, TaHY5-7A, which lacks a transactivation domain, was not sufficient to activate the expression of PURPLE PERICARP-MYB 1 (TaPpm1), the key anthocyanin biosynthesis regulator in purple pericarps of wheat. TaHY5-7A physically interacted with TaBBX-3B in yeast two-hybrid and bimolecular fluorescence complementation assays. Additionally, TaHY5-7A, together with TaBBX-3B, greatly enhanced the promoter activity of TaPpm1 in a dual luciferase assay. Overall, our results suggest that TaHY5-7A and TaBBX-3B collaboratively activate TaPpm1 expression to promote light-induced anthocyanin biosynthesis in purple-pericarp wheat.

OsFAR1 is involved in primary fatty alcohol biosynthesis and promotes drought tolerance in rice.

MAIN CONCLUSION: OsFAR1 encodes a fatty acyl-CoA reductase involved in biosynthesis of primary alcohols and plays an important role in drought stress response in rice. Cuticular waxes cover the outermost surface of terrestrial plants and contribute to inhibiting nonstomatal water loss and improving plant drought resistance. Primary alcohols are the most abundant components in the leaf cuticular waxes of rice (Oryza sativa), but the biosynthesis and regulation of primary alcohol remain largely unknown in rice. Here, we identified and characterized an OsFAR1 gene belonging to the fatty acyl-CoA reductases (FARs) via a homology-based approach in rice. OsFAR1 was activated by abiotic stresses and abscisic acid, resulting in increased production of primary alcohol in rice. Heterologous expression of OsFAR1 enhanced the amounts of C22:0 and C24:0 primary alcohols in yeast (Saccharomyces cerevisiae) and C24:0 to C32:0 primary alcohols in Arabidopsis. Similarly, OsFAR1 overexpression significantly increased the content of C24:0 to C30:0 primary alcohols on rice leaves. Finally, OsFAR1 overexpression lines exhibited reduced cuticle permeability and enhanced drought tolerance in rice and Arabidopsis. Taken together, our results demonstrate that OsFAR1 is involved in rice primary alcohol biosynthesis and plays an important role in responding to drought and other environmental stresses.

Food-water-land-ecosystem nexus in typical Chinese dryland under different future scenarios.

Healthy coupling of the food-water-land-ecosystem (FWLE) nexus is the basis for achieving sustainable development (SD), and FWLE in drylands is frontier scientific issues in the study of coupled human land systems. To comprehensively safeguard the future food, water, and ecological security of drylands, this study examined the implications for FWLE linkages in a typical Chinese dryland from the perspective of future land-use change. First, four different land-use scenarios were proposed using a land-use simulation model with a gray multi-objective algorithm, including an SD scenario. Then, the variation of three ecosystem services was explored: water yield, food production, and habitat quality. Finally, redundancy analysis was used to derive the future drivers of FWLE and explore their causes. The following results were obtained. In the future in Xinjiang, under the business as usual scenario, urbanization will continue, forest area will decrease, and water production will decline by 371 million m3. In contrast, in the SD scenario, this negative impact will be substantially offset, water scarcity will be alleviated, and food production will increase by 1.05 million tons. In terms of drivers, the anthropogenic drivers will moderate the future urbanization of Xinjiang to some extent, with natural drivers dominating the sustainable development scenario by 2030 and a potential 22 % increase in the drivers of precipitation. This study shows how spatial optimization can help protect the sustainability of the FWLE nexus in drylands and simultaneously provides clear policy recommendations for regional development.

Significant loss of soil inorganic carbon at the continental scale.

Widespread soil acidification due to atmospheric acid deposition and agricultural fertilization may greatly accelerate soil carbonate dissolution and CO2 release. However, to date, few studies have addressed these processes. Here, we use meta-analysis and nationwide-survey datasets to investigate changes in soil inorganic carbon (SIC) stocks in China. We observe an overall decrease in SIC stocks in topsoil (0-30 cm) (11.33 g C m-2 yr-1) from the 1980s to the 2010s. Total SIC stocks have decreased by ∼8.99 ± 2.24% (1.37 ± 0.37 Pg C). The average SIC losses across China (0.046 Pg C yr-1) and in cropland (0.016 Pg C yr-1) account for ∼17.6%-24.0% of the terrestrial C sink and 57.1% of the soil organic carbon sink in cropland, respectively. Nitrogen deposition and climate change have profound influences on SIC cycling. We estimate that ∼19.12%-19.47% of SIC stocks will be further lost by 2100. The consumption of SIC may offset a large portion of global efforts aimed at ecosystem carbon sequestration, which emphasizes the importance of achieving a better understanding of the indirect coupling mechanisms of nitrogen and carbon cycling and of effective countermeasures to minimize SIC loss.

Tomato SlCER1-1 catalyzes the synthesis of wax alkanes which increases the drought tolerance and fruit storability.

Very-long-chain (VLC) alkanes are the main wax compounds of tomato fruit and leaf. ECERIFERUM1 (CER1) and ECERIFERUM3 (CER3) are the two key genes involved in VLC alkane biosynthesis in Arabidopsis thaliana. However, the CER1 and CER3 homologous genes in tomato have not been investigated and their exact biological function remains unknown. We analyzed the wax profiles in tomato leaves and fruits at different growth stages, and characterized the CER1 and CER3 homologous genes. VLC alkanes were the predominant wax compounds both in the leaf and fruit at all developmental stages. We identified five CER1 homologs and two CER3 homologs in tomato, which were designated as SlCER1-1 to SlCER1-5 and SlCER3-1 and SlCER3-2 respectively. The genes exhibited tissue- and organ-dependent expression patterns and were induced by abiotic stresses. SlCER1-1 was localized to the endoplasmic reticulum (ER), which is also the main site of wax biosynthesis. Silencing the SlCER1-1 gene in tomato significantly reduced the amounts of n-Alkanes and branched alkanes, whereas its overexpression in Arabidopsis had the opposite effect. Under drought stress, both n-Alkanes and branched alkanes increased significantly in wild-type but not the SlCER1-1 RNAi tomato plants. Furthermore, SlCER1-1 silencing also increased the cuticular permeabilities of the leaves and fruits. In conclusion, SlCER1-1 is involved in wax alkane biosynthesis in tomato and plays an important role in the drought tolerance and fruit storability.

Characterization of an alkylresorcinol synthase that forms phenolics accumulating in the cuticular wax on various organs of rye (Secale cereale).

Alkylresorcinols are bioactive compounds produced in diverse plant species, with chemical structures combining an aliphatic hydrocarbon chain and an aromatic ring with characteristic hydroxyl substituents. Here, we aimed to isolate and characterize the enzyme that forms the alkylresorcinols accumulating in the cuticular wax on the surface of all above-ground organs of rye. Based on sequence homology with other type-III polyketide synthases, a candidate alkylresorcinol synthase was cloned. Yeast heterologous expression showed that the enzyme, ScARS, is highly specific for the formation of the aromatic resorcinol ring structure, through aldol condensation analogous to stilbene synthases. The enzyme accepts long-chain and very-long-chain acyl-CoA starter substrates, preferring saturated over unsaturated chains. It typically carries out three rounds of condensation with malonyl-CoA prior to cyclization, with only very minor activity for a fourth round of malonyl-CoA condensation and cyclization to 5-(2'-oxo)-alkylresorcinols or 5-(2'-hydroxy)-alkylresorcinols. Like other enzymes involved in cuticle formation, ScARS is localized to the endoplasmic reticulum. ScARS expression patterns were found correlated with alkylresorcinol accumulation during leaf development and across different rye organs. Overall, our results thus suggest that ScARS synthesizes the cuticular alkylresorcinols found on diverse rye organ surfaces.

Expression Analysis and Functional Characterization of CER1 Family Genes Involved in Very-Long-Chain Alkanes Biosynthesis in Brachypodium distachyon.

Cuticular wax accumulation and composition affects drought resistance in plants. Brachypodium distachyon plants subjected to water deficit and polyethylene glycol treatments resulted in a significant increase in total wax load, in which very-long-chain (VLC) alkanes were more sensitive to these treatments than other wax compounds, implying that VLC alkanes biosynthesis plays a more important role in drought resistance in B. distachyon. ECERIFERUM1 (CER1) has been reported to encode a core enzyme involved in VLC alkanes biosynthesis in Arabidopsis (Arabidopsis thaliana), but few corresponding genes are investigated in B. distachyon. Here, we identified eight CER1 homologous genes in B. distachyon, namely BdCER1-1 to BdCER1-8, and then analyzed their sequences feature, expression patterns, stress induction, and biochemical activities. These genes had similar protein structure to other reported CER1 and CER1-like genes, but displayed closer phylogenetic relationship to the rice OsGL1 genes. They were further found to exhibit various tissue expression patterns after being induced by abiotic stresses. Among them, BdCER1-8 gene showed extremely high expression in leaves. Heterologous introduction of BdCER1-8 into the Arabidopsis cer1 mutant rescued VLC alkanes biosynthesis. These results indicate that BdCER1 genes are likely to be involved in VLC alkanes biosynthesis of B. distachyon. Taken together, BdCER1-8 seems to play an explicit and predominant role in VLC alkanes biosynthesis in leaf. Our work provides important clues for further characterizing function of CER1 homologous genes in B. distachyon and also an option to improve drought resistance of cereal crops.

TaCER1-1A is involved in cuticular wax alkane biosynthesis in hexaploid wheat and responds to plant abiotic stresses.

To protect above-ground plant organs from excessive water loss, their surfaces are coated by waxes. The genes involved in wax formation have been investigated in detail in Arabidopsis but scarcely in crop species. Here, we aimed to isolate and characterize a CER1 enzyme responsible for formation of the very long-chain alkanes present in high concentrations especially during late stages of wheat development. On the basis of comparative wax and transcriptome analyses of various wheat organs, we selected TaCER1-1A as a primary candidate and demonstrated that it was located to the endoplasmic reticulum, the subcellular compartment for wax biosynthesis. A wheat nullisomic-tetrasomic substitution line lacking TaCER1-1A had significantly reduced amounts of C33 alkane, whereas rice plants overexpressing TaCER1-1A showed substantial increases of C25 -C33 alkanes relative to wild type control. Similarly, heterologous expression of TaCER1-1A in Arabidopsis wild type and the cer1 mutant resulted in increased levels of unbranched alkanes, iso-branched alkanes and alkenes. Finally, the expression of TaCER1-1A was found activated by abiotic stresses and abscisic acid treatment, resulting in increased production of alkanes in wheat. Taken together, our results demonstrate that TaCER1-1A plays an important role in wheat wax alkane biosynthesis and involved in responding to drought and other environmental stresses.

Five Fatty Acyl-Coenzyme A Reductases Are Involved in the Biosynthesis of Primary Alcohols in Aegilops tauschii Leaves.

The diploid Aegilops tauschii is the D-genome donor to hexaploid wheat (Triticum aestivum) and represents a potential source for genetic study in common wheat. The ubiquitous wax covering the aerial parts of plants plays an important role in protecting plants against non-stomatal water loss. Cuticular waxes are complex mixtures of very-long-chain fatty acids, alkanes, primary and/or secondary alcohols, aldehydes, ketones, esters, triterpenes, sterols, and flavonoids. In the present work, primary alcohols were identified as the major components of leaf cuticular wax in Ae. tauschii, with C26:0-OH being the dominant primary alcohol. Analysis by scanning electron microscope revealed that dense platelet-shaped wax crystals were deposited on leaf surfaces of Ae. tauschii. Ten putative wax biosynthetic genes encoding fatty acyl-coenzyme A reductase (FAR) were identified in the genome of Ae. tauschii. Five of these genes, Ae.tFAR1, Ae.tFAR2, Ae.tFAR3, Ae.tFAR4, and Ae.tFAR6, were found expressed in the leaf blades. Heterologous expression of the five Ae.tFARs in yeast (Saccharomyces cerevisiae) showed that Ae.tFAR1, Ae.tFAR2, Ae.tFAR3, Ae.tFAR4, and Ae.tFAR6 were predominantly responsible for the accumulation of C16:0, C18:0, C26:0, C24:0, and C28:0 primary alcohols, respectively. In addition, nine Ae.tFAR paralogous genes were located on D chromosome of wheat and the wheat nullisomic-tetrasomic lines with the loss of Ae.tFAR3 and Ae.tFAR4 paralogous genes had significantly reduced levels of primary alcohols in the leaf blades. Collectively, these data suggest that Ae.tFAR1, Ae.tFAR2, Ae.tFAR3, Ae.tFAR4, and Ae.tFAR6 encode alcohol-forming FARs involved in the biosynthesis of primary alcohols in the leaf blades of Ae. tauschii. The information obtained in Ae. tauschii enables us to better understand wax biosynthesis in common wheat.

Three TaFAR genes function in the biosynthesis of primary alcohols and the response to abiotic stresses in Triticum aestivum.

Cuticular waxes play crucial roles in protecting plants against biotic and abiotic stresses. They are complex mixtures of very-long-chain fatty acids and their derivatives, including C20-C32 fatty alcohols. Here, we report the identification of 32 FAR-like genes and the detailed characterization of TaFAR2, TaFAR3 and TaFAR4, wax biosynthetic genes encoding fatty acyl-coenzyme A reductase (FAR) in wheat leaf cuticle. Heterologous expression of the three TaFARs in wild-type yeast and mutated yeast showed that TaFAR2, TaFAR3 and TaFAR4 were predominantly responsible for the accumulation of C18:0, C28:0 and C24:0 primary alcohols, respectively. Transgenic expression of the three TaFARs in tomato fruit and Arabidopsis cer4 mutant led to increased production of C22:0-C30:0 primary alcohols. GFP-fusion protein injection assay showed that the three encoded TaFAR proteins were localized to the endoplasmic reticulum (ER), the site of wax biosynthesis. The transcriptional expression of the three TaFAR genes was induced by cold, salt, drought and ABA. Low air humidity led to increased expression of TaFAR genes and elevated wax accumulation in wheat leaves. Collectively, these data suggest that TaFAR2, TaFAR3 and TaFAR4 encode active alcohol-forming FARs involved in the synthesis of primary alcohol in wheat leaf and the response to environmental stresses.

Element Geochemistry of Lake Sediments from L07-10 in the "Great Ear" Area of Lop Nur and Its Significance for Climate Variation Change with ICP-OES.

Spectral analysis techniques were applied to geochemical element analysis to provide additional environmental data about evolution of salt lakes and climate change. The elements composition of lake sediments from L07-10 in the "Great Ear" Area of Lop Nur was analyzedby using Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES )and from 14 C carbon dating by using the EN accelerator mass spectrometry (AMS).This paper estimated the climate change in this region since 16.34 ka BP. Results demonstrate that the geochemical characteristics of major elements at all sediment levels can indicate environmental change sensitivity, and element composition is more sensitive to climate change. Overall, climate succession since 16.34 ka BP had been: warm-wet, warm-dry, cold-wet and warm-dry. From 8.09~6.34 ka BP，the climate obviously heated up and was warmer and drier, which is consistent with global and regional climate change estimates from other studies. By about 2 ka BP, the climate becomes warmer and drier and the water carrying capacity of this lake was reduced.

[Study on relationship between alfalfa canopy spectral reflectance and leaf water content].

In the present study, alfalfa canopy reflectance was researched at alfalfa squaring period under different irrigation amount at the hutubi county grassland ecological station. Determining the spectral diagnostic model of alfalfa leaf moisture content was determined by spectrometry. The results showed that (1) The spectral reflectance of alfalfa canopy gradually decreases with the increase in the leaf water content in the near infrared. (2) The spectral inversion model of alfalfa leaf moisture content established by normalized reflectance spectra is superior to the original reflectance spectra, and the prediction model established in the 1,344-1,660 nm band has the lowest average relative error (7.8%). (3) In this study, the spectral diagnostic model of the leaf moisture content is: Y=0.962 - 7.560X1451 + 5.295X1473. The spectral prediction model of the alfalfa leaf moisture content can provide a basis for decision making for scientific irrigation of alfalfa.

[Measuring soil water content by using near infrared spectral characteristics of soil].

Different textured soils (sandy loam, silty clay loam and clay) from Manas County, Xinjiang were researched with indoor spectral reflectance, continuum removal was used to process soil spectra curve, and correlation analysis was made about normalized spectral reflectance and water content for modeling. The results show that different textured soils have reflectance in a order that clay>silty clay loam>sandy loam; the critical points of field capacity in sandy loam, silty clay loam and clay were 20.01%, 24.10% and 30.43% respectively, and water content was inversely proportional to spectral reflectance below such critical points while proportional above the points. Within 1390-1623 nm band, the negative correlation coefficients of soil water content and normalized spectra reflectance show better negative correlation and reaching significant levels, R2 of the model established for soil water content prediction exceeded mostly 0.8, and the average relative error of the model was 10%. The model could accurately reflect the soil moisture content, Its advantages such as accuracy, non-destruction and rapidness provide a new approach to measuring soil water content.

[Analysis of Cd pollution & spatial variability characteristics of typical agricultural soils in suburbs of Urumqi City, Xinjiang].

Cd content in agricultural soils from 4 townships in north suburb and 2 townships in south suburb of Urumqi City was determined with graphite furnace (GF-990) atomic absorption spectrometry, and the spatial variability of Cd content there was analyzed with geo-statistics method. The result indicates that Cd content from the sampling points in north suburb exceeds the stipulated standard and falls into medium or strong spatial variability with nugget value > 75%, which explains a weak self-correlation among those variable spaces under mainly the impact of random factors of external pollution such as local fertilization level, irrigation and cultivation etc. Cd content from the sampling points in north suburb does not exceed the stipulated standard but approaches to the critical warning value with nugget value at 50.2%, which falls into medium self-correlation among those variable spaces. Spatial variability characteristics of Cd distribution in suburban agricultural soils of Urumqi City, Xinjiang were studied in this paper for providing scientific basic data to source analysis and control of Cd pollution in the far mLands there.