Construction of a dual "off-on" near-infrared fluorescent probe for bioimaging of HClO in rheumatoid arthritis.

Hypochlorous acid (HClO) serves as a critical biomarker in inflammatory diseases such as rheumatoid arthritis (RA), and its real-time imaging is essential for understanding its biological functions. In this study, we designed and synthesized a novel probe, RHMB, which ingeniously integrates rhodamine B and methylene blue fluorophores with HClO-specific responsive moieties into a single molecular framework. Upon exposure to HClO, RHMB exhibited significant dual-channel fluorescence enhancement characterized by high sensitivity (LODs of 2.55 nM and 14.08 nM), excellent selectivity, and rapid response time (within 5 s). Notably, RHMB enabled reliable imaging of both exogenous and endogenous HClO in living cells and in zebrafish, employing a unique duplex-imaging turn-on approach that highlighted its adaptability across various biological contexts. Furthermore, RHMB effectively monitored HClO fluctuations in an RA mouse model and assessed the therapeutic efficacy of diclofenac (Dic) in alleviating RA symptoms. These findings underscore the potential of RHMB as an invaluable tool for elucidating the biological roles of HClO in various diseases.

Anisotropic Microstructure and Performance Characterization of Wild Silkworm Cocoons for Designing Biomimetic Protective Materials.

As a unique and important biopolymer composite, silkworm cocoons have evolved a wide range of different structures and combinations of physical and chemical properties to resist environmental damage and attacks from natural predators. A combination of characterization techniques including scanning electron microscopy, mechanical tests, and Fourier transform infrared spectroscopy were applied to investigate the morphologies, mechanical properties, and nanoscale organizations of Antheraea pernyi cocoons from two different source regions. Mechanical tests were carried out by using rectangular specimens cut from four directions 0° (width of the cocoons), ±45°, and 90° (the length of the cocoon), separately. The mechanical properties such as tensile strength, initial modulus, and maximum load of cocoon in four directions were measured. The structural analysis of silkworm cocoon shows that there is a slightly different combination of morphology and properties that have adapted to coping with diverse local environments. The results of the mechanical properties of silkworm cocoons show that the A. pernyi cocoon from north of China behaved stronger and tougher. Besides, there were slight differences among the results of mechanical properties for 0°, ±45°, and 90° directions of these cocoons. Our studies will help formulate bio-inspired design principles for new materials.

SRPX2 boosts pancreatic cancer chemoresistance by activating PI3K/AKT axis.

BACKGROUND AND AIM: This investigation was aimed at disclosing whether SRPX2 affected pancreatic cancer (PC) chemoresistance by regulating PI3K/Akt/mTOR signaling. METHODS: Totally 243 PC patients were recruited, and they were incorporated into partial remission (PR) group, stable disease (SD) group and progressive disease (PD) group in accordance with their chemotherapeutic response. PC cell lines (i.e. AsPC1, Capan2, VFPAC-1, HPAC, PANC-1, BxPC-3 and SW1990) and human pancreatic ductal epithelial cell lines (hTERT-HPNE) were also collected. RESULTS: PC patients of SD + PD group were associated with higher post-chemotherapeutic SRPX2 level than PR group, and their post-chemotherapeutic SRPX2 level was above the pretherapeutic SRPX2 level (P < 0.05). PR population showed lower SRPX2 level after chemotherapy than before chemotherapy (P < 0.05). Besides high serum SRPX2 level and SRPX2 level change before and after chemotherapy were independent predictors of poor PC prognosis. Additionally, si-SRPX2 enhanced chemosensitivity of PC cell lines, and expressions of p-PI3K, p-AKT and p-mTOR were suppressed by si-SRPX2 (P < 0.05). IGF-1 treatment could changeover the impact of si-SRPX2 on proliferation, migration, invasion and chemoresistance of PC cells (P < 0.05). CONCLUSION: The SRPX2-PI3K/AKT/mTOR axis could play a role in modifying progression and chemoresistance of PC cells, which might help to improve PC prognosis.