Efficient nitrate and Cr(VI) removal by denitrifier: The mechanism of S. oneidensis MR-1 promoting electron production, transportation and consumption.

When Cr(VI) and nitrate coexist, the efficiency of both bio-denitrification and Cr(VI) bio-reduction is poor because chromate hinders bacterial normal functions (i.e., electron production, transportation and consumption). Moreover, under anaerobic condition, the method about efficient nitrate and Cr(VI) removal remained unclear. In this paper, the addition of Shewanella oneidensis MR-1 to promote the electron production, transportation and consumption of denitrifier and cause an increase in the removal of nitrate and Cr(VI). The efficiency of nitrate and Cr(VI) removal accomplished by P. denitrificans as a used model denitrifier increased respectively from 51.3% to 96.1% and 34.3% to 99.8% after S. oneidensis MR-1 addition. The mechanism investigations revealed that P. denitrificans provided S. oneidensis MR-1 with lactate, which was utilized to secreted riboflavin and phenazine by S. oneidensis MR-1. The riboflavin served as coenzymes of cellular reductants (i.e., thioredoxin and glutathione) in P. denitrificans, which created favorable intracellular microenvironment conditions for electron generation. Meanwhile, phenazine promoted biofilm formation, which increased the adsorption of Cr(VI) on the cell surface and accelerated the Cr(VI) reduction by membrane bound chromate reductases thereby reducing damage to other enzymes respectively. Overall, this strategy reduced the negative effect of chromate, thus improved the generation, transportation, and consumption of electrons. SYNOPSIS: The presence of S. oneidensis MR-1 facilitated nitrate and Cr(VI) removal by P. denitrificans through decreasing the negative effect of chromate due to the metabolites' secretion.

Metabolite Cross-Feeding Promoting NADH Production and Electron Transfer during Efficient SMX Biodegradation by a Denitrifier and S. oneidensis MR-1 in the Presence of Nitrate.

Antibiotics often coexist with other pollutants (e.g., nitrate) in an aquatic environment, and their simultaneous biological removal has attracted widespread interest. We have found that sulfamethoxazole (SMX) and nitrate can be efficiently removed by the coculture of a model denitrifier (Paracoccus denitrificans, Pd) and Shewanella oneidensis MR-1 (So), and SMX degradation is affected by NADH production and electron transfer. In this paper, the mechanism of a coculture promoting NADH production and electron transfer was investigated by proteomic analysis and intermediate experiments. The results showed that glutamine and lactate produced by Pd were captured by So to synthesize thiamine and heme, and the released thiamine was taken up by Pd as a cofactor of pyruvate and ketoglutarate dehydrogenase, which were related to NADH generation. Additionally, Pd acquired heme, which facilitated electron transfer as heme, was the important composition of complex III and cytochrome c and the iron source of iron sulfur clusters, the key component of complex I in the electron transfer chain. Further investigation revealed that lactate and glutamine generated by Pd prompted So chemotactic moving toward Pd, which helped the two bacteria effectively obtain their required substances. Obviously, metabolite cross-feeding promoted NADH production and electron transfer, resulting in efficient SMX biodegradation by Pd and So in the presence of nitrate. Its feasibility was finally verified by the coculture of an activated sludge denitrifier and So.

The potential risks posed by micro-nanoplastics to the safety of disinfected drinking water.

Micro-nanoplastics (M-NPs) have become an emerging critical issue in the environment because they migrate easily, can bioaccumulate with toxic effects, and are difficult to degrade. Unfortunately, the current technologies for removing or degrading M-NPs in drinking water are insufficient to eliminate them completely, and residual M-NPs in drinking water may pose a threat to human health by impairing human immunity and metabolism. In addition to their intrinsic toxic effects, M-NPs may be even more harmful after drinking water disinfection than before disinfection. Herein, this paper comprehensively summarizes the negative impacts of several commonly used disinfection processes (ozone, chlorine, and UV) on M-NPs. Moreover, the potential leaching of dissolved organics from M-NPs and the production of disinfection byproducts during the disinfection process are discussed in detail. Moreover, due to the diversity and complexity of M-NPs, their adverse effects may exceed those of conventional organics (e.g., antibiotics, pharmaceuticals, and algae) after the disinfection process. Finally, we propose enhanced conventional drinking water treatment processes (e.g., enhanced coagulation, air flotation, advanced adsorbents, and membrane technologies), detection of residual M-NPs, and biotoxicological assessment as promising and ecofriendly candidates to efficiently remove M-NPs and avoid the release of secondary hazards.

Propionibacterium freudenreichii-Assisted Approach Reduces N2O Emission and Improves Denitrification via Promoting Substrate Uptake and Metabolism.

N2O emission is often encountered during biodenitrification. In this paper, a new approach of using microorganisms to promote substrate uptake and metabolism to reduce denitrification intermediate accumulation was reported. With the introduction of Propionibacterium freudenreichii to a biodenitrification system, N2O and nitrite accumulation was, respectively, decreased by 74 and 60% and the denitrification efficiency was increased by 150% at the time of 24 h with P. freudenreichii/groundwater denitrifier of 1/5 (OD600). Propionate, produced by P. freudenreichii, only accelerated nitrate removal and was not the main reason for the decreased intermediate accumulation. The proteomic and enzyme analyses revealed that P. freudenreichii stimulated biofilm formation by upregulating proteins involved in porin forming, putrescine biosynthesis, spermidine/putrescine transport, and quorum sensing and upregulated transport proteins, which facilitated the uptake of the carbon source, nitrate, and Fe and Mo (the required catalytic sites of denitrification enzymes). Further investigation revealed that P. freudenreichii activated the methylmalonyl-CoA pathway in the denitrifier and promoted it to synthesize heme/heme d1, the groups of denitrification enzymes and electron transfer proteins, which upregulated the expression of denitrifying enzyme proteins and enhanced the ratio of NosZ to NorB, resulting in the increase of generation, transfer, and consumption of electrons in biodenitrification. Therefore, a significant reduction in the denitrification intermediate accumulation and an improvement in the denitrification efficiency were observed.

The effects of biodegradation on the characteristics and disinfection by-products formation of soluble microbial products chemical fractions.

Soluble microbial products (SMPs) discharged into rivers from sewage treatment plants may increase the health risk for downstream drinking water by acting as a precursor of DBPs. Biotransformation or biodegradation could alter the characteristics of SMPs and affect the subsequent formation of DBPs. This study observed the relative contribution of chemical fractions in SMPs and explored the biodegradation of each fraction and their effect on disinfection by-products (DBPs) formation in surface water. The hydrophilic acid (HPIA) and hydrophobic acid (HPOA) constituted the major portion of the SMPs, which were dominated by fulvic acid and humic acids. The transphilic acid (TPIA) and hydrophobic bases (HPOB) were relatively minor but it contained a relative substantial portion of protein-like materials in SMPs. TPIA and HPOB produced insignificant amounts of DBP corresponding to 13% and 14% in the original samples, but they were collectively responsible for 50% of the DBPs yield. Much larger amounts of hydrophobic fractions were utilized than hydrophilic fractions after biodegradation. The increase in SUVA values indicating aromatic structures, except for HPOA fraction, was observed after biodegradation. The protein-like materials in both the HPOA and HPIA fractions and polycarboxylate-type humic acid in the HPIA fraction decreased but the enrichment of HPOA (MW > 100 kDa) and TPIA (MW < 1 kDa) was observed after biodegradation. The production of = C-H in HPIA fraction and the appearance of double peak at 1100 cm-1 in TPIA and HPOB fractions occurred after biodegradation. In overall level, microorganisms effectively utilized DBP precursors from HPIA, HPOA and HPOB fractions but increased the DBPs precursors from the TPIA fraction. TPIA and HPOB fractions had higher DBP yield with chlorine but the DBPs yield of HPIA and HPOA changed little after biodegradation.

Probing size characteristics of disinfection by-products precursors during the bioavailability study of soluble microbial products using ultrafiltration fractionation.

Soluble microbial products (SMPs) discharged into surface water may increase the formation of disinfection by-products (DBPs) in downstream drinking water treatment plants. In this study, ultrafiltration (UF) fractionation was used to separate SMPs into homogenous components. An aerobic microbial experiment was conducted to evaluate the bioavailability of individual molecular weight (MW) fractions of SMPs in surface water and the impact on their DBP formation, facilitating the interpretation of SMPs characterization and DBPs reactivity. For SMPs, organics with MW < 1 kDa were the primary fraction, containing the most abundant humic substances. The 30 kDa < MW < 100 kDa fraction was the lowest in SMPs but had the highest SUVA values. After biodegradation, the bioavailability of physical fractions increased with the increasing MW size. However, the SUVA value, except for MW < 1 kDa, increased in individual fraction after biodegradation. Low molecular weight SMPs fractions (MW<10 kDa) were major precursors for DBP in which trichloromethane (TCM) was the most abundant. The 10 kDa  100KDa had relative abundant dichloroacetonitrile (DCAN) formation. After biodegradation, TCM precursors with MW < 1 kDa were removed by approximately 20%, whereas the increase of TCM formation was observed in 1 kDa < MW < 100 kDa fraction. CH formation from 1 kDa < MW < 10 kDa increased considerably, but those from 10 kDa < MW < 30 kDa decreased after biodegradation, as a result of the biotransformation of large organic acids to small organic acids. In terms of DBP reactivity, the TCM yield for the MW < 1 kDa fraction had no significant change while the 30 kDa < MW < 100 kDa fraction exhibited the greatest increase (approximately 8 times) in TCM yield.

Biosorption behavior of the Ochrobactrum MT180101 on ionic copper and chelate copper.

To study the biosorption behaviors of bacteria on heavy metal chelators, the biosorption kinetics, biosorption thermodynamics and pH influence tests of the Ochrobactrum MT180101 on ionic and chelate copper were investigated. Furthermore, the biosorption mechanisms of the Ochrobactrum MT180101 on ionic copper and chelate copper were explained by means of an excitation emission matrix as well as infrared and X-ray photoelectron spectroscopy. The results indicated the following. 1) The biosorption on chelate copper was needed to destroy the complexation group first through metabolic and secretory activities. 2) The biosorption mechanism of the Ochrobactrum MT180101 on copper involved surface biosorption, extracellular chelation and bienzyme-mediated biotransformation. The results suggested that Ochrobactrum had a superior biosorption efficiency to ionic and chelate copper.

Adsorption of soluble microbial products by sediments.

As major precursors of disinfection by-products (DBPs), soluble microbial products (SMPs) generated by sewage discharge can adversely affect drinking water quality. It is essential to understand the adsorption behaviours of SMPs onto sediments and the effect of DBPs formation. In this study, the adsorption ability of sediments was evaluated by adsorption isotherms with respect to temperature and salinity. Adsorption behaviours were investigated using X-ray photoelectron spectroscopy, electron microscopy analysis, and excitation emission matrix fluorescence analysis. Chlorination was also employed to explore the influence of sediment adsorption on drinking water quality. The results indicated that the maximum adsorption potential of sediments to SMPs was 1.60 mg/g, which involved exothermic processes. SMPs adsorption declined with increasing temperature and salinity, and fulvic acid and protein in SMPs were more readily adsorbed on sediments than was humic acid. Correlation analysis results indicated that adsorption behaviours of sediments to SMPs could significantly reduce the generation potential of DBPs (r = 0.882-0.938, p < 0.01). In addition, the decrease of C-DBPs was considerably greater than that of N-DBPs. These research findings are of importance to assessments of the fate and transport of SMPs in water-sediment systems, as well as the effect of following DBPs formation in the drinking water supply.

Bioavailability of soluble microbial products as the autochthonous precursors of disinfection by-products in aerobic and anoxic surface water.

Soluble microbial products (SMPs), as a major part of the effluent organic matter discharged into surface water, may affect the formation of disinfection by-products (DBP) in downstream drinking water treatment plants. In this study, excitation emission matrix fluorescence with parallel factor analysis (EEM-PARAFAC), infrared spectroscopy (IR), high performance size-exclusion chromatography (HPSEC) and 16SrRNA high-throughput sequencing were used to investigate the aerobic and anoxic bioavailability of SMPs in surface water and evaluate their influences on DBP formation upon chlorination in a subsequent drinking water plant. In this study, SMPs were utilized by enriched microbial communities such as Bacteroidetes and Proteobacteria, but the accumulation of SUVA was pronounced during the two oxygen conditions. Biodegraded SMPs had higher humic substructures and lower protein-like components. Due to the presence of SMPs, microbial community compositions were influenced during biodegradation. Moreover, DO was the main factor in biodegradation of SMPs, thus affecting a series of processes, such as microbial compositions, properties of SMPs, DBP formation and reactivity. DBP formation potential decreased after anoxic and aerobic incubations. However, SMPs after aerobic degradation had higher DBP reactivity meanwhile the opposite was found for anoxic incubation. Based on the analysis of IR and HPSEC, it was found that some new substrates or intermediates with MW (220 KDa, <1 KDa) during microbial incubation may contribute to the formation of trihalomethane (THMs), chloral hydrate (CH), dichloroacetonitrile (DCAN) and trichloronitromethane (TCNM) in each DBP sampling episode.

Copper-resistant mechanism of Ochrobactrum MT180101 and its application in membrane bioreactor for treating electroplating wastewater.

It is necessary to study the mechanism of resistance to heavy metals in microbiological processes. In this study, Ochrobactrum MT180101 was used as the microbial source of an membrane bioreactor to investigate its degradation efficiency for electroplating wastewater and the copper-resistant mechanism. Meanwhile, excitation emission matrix-parallel factor, scanning electron microscope, atomic force microscope, fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and proteome analyses were applied to explain the comprehensive mechanism of the Ochrobactrum MT180101 resisting heavy metal toxicity. The results indicated that the Ochrobactrum MT180101 resisted heavy metal toxicity with the following pathways: i) binding metal cations on cell wall surfaces, ii) generating microbial products such as protein to chelate and stabilize the metal cations, iii) bio-transporting heavy metals from the intramembrane to the outer membrane by means of intracellular transport, and iv) reducing heavy metals through enzyme-mediated biotransformation. The results ensure that Ochrobactrum MT180101 was a copper-resistant bacterium that can be used in the pretreatment or deep treatment of electroplating wastewater.

Solar photolysis of soluble microbial products as precursors of disinfection by-products in surface water.

In the Pearl River Delta area, the upstream municipal wastewater is commonly discharged into rivers which are a pivotal source of downstream drinking water. Solar irradiation transforms some of the dissolved organic matter discharged from the wastewater, also affecting the formation of disinfection by-products in subsequent drinking water treatment plants. The effect of simulated solar radiation on soluble microbial products extracted from activated sludge was documented in laboratory experiments. Irradiation was found to degrade macromolecules in the effluent, yielding smaller, more reactive intermediate species which reacted with chlorine or chloramine to form higher levels of noxious disinfection by-products. The soluble microbial products were found to be more active in formation of disinfection by-products regard than naturally-occurring organic matter. The results show that solar irradiation induced the formation of more trihalomethane (THMs), chloral hydrate (CH) and trichloronitromethane (TCNM), causing greater health risks for downstream drinking water.