Foam fractionation for effective removal of Pseudomonas aeruginosa from water body: Strengthening foam drainage by artificially inducing foam evolution.

Lack of microbial contamination is of great significance to drinking water safety and water reclamation. In this work, foam fractionation was employed to remove Pseudomonas aeruginosa (P. aeruginosa) from aqueous solution and dodecyl dimethyl betaine (BS12) was used as the collector. Since the attachment of strain cells on the bubble surface would impede the reflux of interstitial liquid in the plateau borders (PBs), a novel strategy in strengthening foam drainage was developed through artificially inducing foam evolution. Two gas distributors with different pore diameters had been mounted at the bottom of the column for regulating the radial distribution of bubble size in the foam phase. Experimental results indicated that gas diffuse and bubble coarsening could be significantly promoted by increasing the size difference among the adjacent bubbles. Bubble coalescence contributed to broadening the width of plateau borders, thereby avoiding the borders blockage by strain cells. During bubble coalescence, surfactant molecules would be partially shifted from the surface of small bubble towards that of large bubble due to the molecule density difference. The increase in surface excess of surfactant molecules on gas-liquid interface was conducive to improving foam stability. Under the suitable conditions of air flow rates of gas distributor with 0.125 mm of pore diameter 75 mL/min and gas distributor with 0.425 mm of pore diameter 125 mL/min, BS12 concentration 0.1 g/L, and P. aeruginosa concentration 2.0 × 104 CFU/mL, the removal percentage and enrichment ratio of P. aeruginosa were 99.6% and 10.6, respectively. This work is expected to provide some new light for strengthening foam drainage in the presence of solid particles and to facilitate the industrialization of foam fractionation in water treatment.

Unfolding the Determinants of COVID-19 Vaccine Acceptance in China.

BACKGROUND: China is at the forefront of global efforts to develop COVID-19 vaccines and has five fast-tracked candidates at the final-stage, large-scale human clinical trials testing phase. Vaccine-promoting policymaking for public engagement is a prerequisite for social mobilization. However, making an informed and judicious choice is a dilemma for the Chinese government in the vaccine promotion context. OBJECTIVE: In this study, public opinions in China were analyzed via dialogues on Chinese social media, based on which Chinese netizens' views on COVID-19 vaccines and vaccination were investigated. We also aimed to develop strategies for promoting vaccination programs in China based on an in-depth understanding of the challenges in risk communication and social mobilization. METHODS: We proposed a novel behavioral dynamics model, SRS/I (susceptible-reading-susceptible/immune), to analyze opinion transmission paradigms on Chinese social media. Coupled with a meta-analysis and natural language processing techniques, the emotion polarity of individual opinions was examined in their given context. RESULTS: We collected more than 1.75 million Weibo messages about COVID-19 vaccines from January to October 2020. According to the public opinion reproduction ratio (R0), the dynamic propagation of those messages can be classified into three periods: the ferment period (R01=1.1360), the revolution period (R02=2.8278), and the transmission period (R03=3.0729). Topics on COVID-19 vaccine acceptance in China include price and side effects. From September to October, Weibo users claimed that the vaccine was overpriced, making up 18.3% (n=899) of messages; 38.1% (n=81,909) of relevant topics on Weibo received likes. On the contrary, the number of messages that considered the vaccine to be reasonably priced was twice as high but received fewer likes, accounting for 25.0% (n=53,693). In addition, we obtained 441 (47.7%) positive and 295 (31.9%) negative Weibo messages about side effects. Interestingly, inactivated vaccines instigated more heated discussions than any other vaccine type. The discussions, forwards, comments, and likes associated with topics related to inactivated vaccines accounted for 53% (n=588), 42% (n=3072), 56% (n=3671), and 49% (n=17,940), respectively, of the total activity associated with the five types of vaccines in China. CONCLUSIONS: Most Chinese netizens believe that the vaccine is less expensive than previously thought, while some claim they cannot afford it for their entire family. The findings demonstrate that Chinese individuals are inclined to be positive about side effects over time and are proud of China's involvement with vaccine development. Nevertheless, they have a collective misunderstanding about inactivated vaccines, insisting that inactivated vaccines are safer than other vaccines. Reflecting on netizens' collective responses, the unfolding determinants of COVID-19 vaccine acceptance provide illuminating benchmarks for vaccine-promoting policies.

Modified multi-walled carbon nanotubes assisted foam fractionation for effective removal of acid orange 7 from the dyestuff wastewater.

In this study, multi-walled carbon nanotubes (MWCNTs) had been used to strengthen the removal of acid orange 7 (AO7) from the dyestuff wastewater by using foam fractionation. First, the surface modification of MWCNTs was performed by introducing hypochlorite groups (-OCl). The modified MWCNTs were characterized by using SEM, XRD, FTIR and Raman spectroscopy. Subsequently, the potential of modified MWCNTs as a novel collector for AO7 adsorption was examined. The adsorption conditions of modified MWCNTs towards AO7 were optimized by using response surface methodology (RSM) with a central composite design (CCD). The adsorption capacity of modified MWCNTs towards AO7 could reach 47.72 ± 0.79 mg·g-1 under the optimum conditions. The kinetics and the equilibrium adsorption data were analyzed by using different kinetic and isotherm models. According to the regression results, adsorption kinetics data were well described by pseudo-second order model, whereas adsorption isotherm data were best represented by Langmuir isotherm model. Finally, foam fractionation was performed with a batch mode. Under the suitable conditions of loading liquid volume 300 mL, modified MWCNTs dosage 180 mg, cetyltrimethylammonium bromide (CTAB) concentration 50 mg·L-1, AO7 concentration 30 mg·L-1, pore diameter of gas distributor 0.125 mm and air flow rate 100 mL·min-1, the removal percentage and enrichment ratio of AO7 were 91.23% and 6.17, respectively. The decolourization ratio of solution after foam fractionation was found to be 98.66%.

Xanthan gum assisted foam fractionation for the recovery of casein from the dairy wastewater.

Casein was the major protein in the milk of mammals and it was massively lost in the discharged wastewater during dairy product manufacture. This work was aimed at recovering casein from the dairy wastewater by using foam fractionation. In order to improve the foam stability, xanthan gum was used as the foam stabilizer based on the association between protein and polysaccharide. The results of the scanning electron microscope (SEM) and fluorescence emission spectrum suggested that casein could be associated with xanthan gum primarily through electrostatic attraction, which was significantly affected by pH. Moreover, the introduction of xanthan gum had a marked impact on the surface tension, foam ability, foam stability, turbidity, zeta potential and average particle size of casein dispersion. Foam fractionation was performed under the suitable operation condition of pH 6.0, loading liquid volume 400 mL, amount ratio of casein and xanthan gum 1:2, volumetric airflow rate 100 mL/min and pore diameter of gas distributor 0.180 mm. The enrichment ratio and the recovery percentage of casein reached as high as 16.81 and 86.51%, respectively. This work is expected to provide a cost-effective method to recover the trace desired material through improving foam stability based on intermolecular forces.Research highlightsFoam fractionation has been proposed to recover casein from dairy wastewater.Xanthan gum has been used to improve the foam stability of casein.The main interaction between casein and xanthan gum was an electrostatic attraction.Xanthan gum contributed to weakening the flowability of interstitial liquid.

Bioleaching assisted foam fractionation for recovery of gold from the printed circuit boards of discarded cellphone.

Present work was focused on recovering gold (Au) from the printed circuit boards (PCBs) of discarded cellphone by bioleaching assisted continuous foam fractionation. First, the cyanide-producing strains of Pseudomonas putida and Bacillus megaterium were co-cultured in order to supply a high cyanide concentration in the nutrient solution for mobilizing Au from waste PCBs (WPCBs). Bioleaching conditions were optimized by using response surface methodology. Under the suitable bioleaching conditions of pH of 10.0, pulp density of 5 g/L and leaching time of 34 h, the Au mobilization percentage was 83.59%. The leaching liquor with an Au concentration of 1.34 mg/L could be used as the feeding solution of continuous foam fractionation after removing solid particles and cell biomass. In order to strengthen foam drainage, a novel internal component of foam fractionation column was developed. Under the suitable operation conditions of CTAB concentration of 0.2 g/L, volumetric air flow rate of 100 mL/min and feed flow rate of 10 mL/min, the enrichment ratio and recovery percentage of Au were 43.62 and 87.46%, respectively. This study is expected to provide an effective strategy to recover Au from WPCBs, and to supplement the depleting natural resources.

In-situ activated nanoparticle as an efficient and recyclable foam stabilizer for enhancing foam separation of LAS.

Linear alkylbenzene sulfonate (LAS) is a particular member of the emerging contaminants, because of its increasingly ubiquitous use and tremendous harm to the environment and wastewater treatment plant. Herein, we develop a novel two-stage foam separation to recover LAS (18.7-91.0 mg/L) from laundry wastewater. We first reported the fabrication of activated silica nanoparticle (SNP) via a facile and scalable in-situ approach. To obtain a desirable surface property, the key design element was the utilization of amphoteric surfactant, viz. cocamidopropyl betaine, as the modifier. In the first-stage, activated SNP could serve as an efficient foam stabilizer to improve the foam stability and the interfacial adsorption of LAS. Remarkably, LAS concentration in effluent decreased to very low levels of 1.9-2.9 mg/L with a proper enrichment ratio, and met the emission standard. In the second-stage, we originally adopted the intensification of liquid drainage to selectively recycle activated SNP from LAS. An inclined foam channel (IFC) covered by hydrophobic coating was constructed and the maximum recovery percentage of SNP reached 91.5%, indicating that the activated SNP was recyclable. This work definitely proves that the integration of nanotechnology in foam separation can make wastewater treatment more efficient and less expensive.

Pyruvate is an effective substitute for glutamate in regulating porcine nitrogen excretion.

This study was performed to determine if pyruvate, which acts as a critical intermediate in energy metabolism, can substitute the role of glutamate as a metabolic fuel and effectively reduce nitrogen excretion in pigs. First, the experiment in vitro was carried out to investigate the effects of culturing porcine small intestinal epithelial cell line with pyruvate on the oxidation. Then, barrows weighing 40 kg were used in the experiment investigating the changes of nitrogen balance in response to addition of pyruvate to low-protein diets. Last, barrows (40 kg), which were surgically fitted with permanent catheters in the mesenteric vein, portal vein, hepatic vein, and carotid artery, were used to investigate the effects of supplementing low-protein diets with calcium pyruvate on the net portal fluxes of amino acids (AAs) and the consumption of AAs in the liver. The results showed that culturing cells with sodium pyruvate significantly reduced the number of glutamate oxidation (P < 0.05). Addition of calcium pyruvate to low-protein diets significantly reduced urinary nitrogen excretion from 13.2 g/d (18.0% crude protein, CP) to 10.3 g/d (15.0% CP) or 7.80 g/d (13.5% CP) and total nitrogen excretion from 22.5 g/d (18.0% CP) to 17.8 g/d (15.0% CP) or 14.2 g/d (13.5% CP) (P < 0.05), without obviously negative effects on the nitrogen retention (P > 0.05). Addition of calcium pyruvate to low-protein diets significantly decreased essential AA consumption rate in the liver (P < 0.05). This diet modification reduced the net portal fluxes of NH3, glycine, and alanine, as well as urea production rate in the liver (P < 0.05). The results indicated that pyruvate is an effective substitute for glutamate as a supplement in low-protein diets, reducing porcine nitrogen excretion and nitrogen consumption.

Low-Protein Diets Decrease Porcine Nitrogen Excretion but with Restrictive Effects on Amino Acid Utilization.

Reducing dietary crude protein (CP) intake effectively decreases nitrogen excretion in growing-finishing pigs but at the expense of poor growth when dietary CP content is reduced by ≥3%. In this study, we investigated the main disadvantages of low-protein diets supplemented with lysine, methionine, threonine, and tryptophan in pigs. First, changes in the nitrogen balance in response to differences in dietary CP content (18%, 15%, and 13.5%) were investigated in barrows (40 kg). Then, barrows (40 kg) surgically fitted with catheters in the mesenteric vein, portal vein, hepatic vein, and carotid artery were used to investigate changes in amino acid (AA) metabolism in the portal-drained viscera and liver in response to differences in dietary CP content. The results showed that low-protein diets reduced fecal and urinary nitrogen excretion ( P < 0.05) meanwhile resulted in significant decreases in nitrogen retention ( P < 0.05). Moreover, a reduction in the dietary CP content from 18% to 13.5% resulted in decreases in the net portal fluxes of NH3, glycine, and alanine as well as in the urea production in the liver ( P < 0.05), whereas their values as a percentage of nitrogen intake did not decline ( P > 0.05). The net portal fluxes of nonessential AA (NEAA) were reduced in the low-protein diet groups ( P < 0.05), while essential AA consumption in the liver increased ( P < 0.05). Thus, low-protein diets result in reductions in both nitrogen excretion and retention, and NEAA deficiency may be a major disadvantage of low-protein diets.

Activation of Pyruvate Dehydrogenase by Sodium Dichloroacetate Shifts Metabolic Consumption from Amino Acids to Glucose in IPEC-J2 Cells and Intestinal Bacteria in Pigs.

The extensive metabolism of amino acids (AA) as fuel is an important reason for the low use efficiency of protein in pigs. In this study, we investigated whether regulation of the pyruvate dehydrogenase kinase (PDK)/pyruvate dehydrogenase alpha 1 (PDHA1) pathway affected AA consumption by porcine intestinal epithelial (IPEC-J2) cells and intestinal bacteria in pigs. The effects of knockdown of PDHA1 and PDK1 with small interfering RNA (siRNA) on nutrient consumption by IPEC-J2 cells were evaluated. IPEC-J2 cells were then cultured with sodium dichloroacetate (DCA) to quantify AA and glucose consumption and nutrient oxidative metabolism. The results showed that knockdown of PDHA1 using siRNA decreased glucose consumption but increased total AA (TAA) and glutamate (Glu) consumption by IPEC-J2 cells ( P < 0.05). Opposite effects were observed using siRNA targeting PDK1 ( P < 0.05). Additionally, culturing IPEC-J2 cells in the presence of 5 mM DCA markedly increased the phosphorylation of PDHA1 and PDH phosphatase 1, but inhibited PDK1 phosphorylation ( P < 0.05). DCA treatment also reduced TAA and Glu consumption and increased glucose depletion ( P < 0.05). These results indicated that PDH was the regulatory target for shifting from AA metabolism to glucose metabolism and that culturing cells with DCA decreased the consumption of AAs by increasing the depletion of glucose through PDH activation.

BS12-assisted flotation for the intensification of SNPs separation from CMP wastewater using a novel flotation column.

In view of the extremely small size, high stable dispersion and intricate colloidal nature of silica nanoparticles (SNPs) in chemical mechanical polishing (CMP) wastewater, they might not only have hazards for environment and human health, but also cause low separation efficiency by classical water-treatment processes. Thus, it would be an important challenge to develop an efficient flotation technology for the separation SNPs. For this propose, this paper firstly presented the interaction between SNPs and dodecyl dimethyl betaine (ambient-friendly surfactant). Secondly, a novel flotation column was developed for strengthening interfacial adsorption by micro-bubbles and enhancing foam drainage by internal of regular-decagonal hollow frustum (RHF). One vital finding was that the mixture of micro-bubbles and macro-bubbles was conducive to improving the flotation performance. Under the suitable operating conditions, the enrichment ratio (E) and recovery percentage (R) of SNPs could reach 30.4±1.5 and 90.8±4.5%, respectively. The great E and R were obtained simultaneously, revealing a good participation of RHF in the flotation. Without a doubt, owing to the low chemical reagent addition and the high flotation performance, it was clear that our flotation has huge implications for the separation of nanoparticles from their wastewaters.

Role of foam drainage in producing protein aggregates in foam fractionation.

It is essential to obtain a clear understanding of the foam-induced protein aggregation to reduce the loss of protein functionality in foam fractionation. The major effort of this work is to explore the roles of foam drainage in protein aggregation in the entire process of foam fractionation with bovine serum albumin (BSA) as a model protein. The results show that enhancing foam drainage increased the desorption of BSA molecules from the gas-liquid interface and the local concentration of desorbed molecules in foam. Therefore, it intensified the aggregation of BSA in foam fractionation. Simultaneously, it also accelerated the flow of BSA aggregates from rising foam into the residual solution along with the drained liquid. Because enhancing foam drainage increased the relative content of BSA molecules adsorbed at the gas-liquid interface, it also intensified the aggregation of BSA during both the defoaming process and the storage of the foamate. Furthermore, enhancing foam drainage more readily resulted in the formation of insoluble BSA aggregates. The results are highly important for a better understanding of foam-induced protein aggregation in foam fractionation.

ß-Cyclodextrin preventing protein aggregation in foam fractionation of bovine serum albumin.

In this work, ß-cyclodextrin was developed to prevent protein aggregation in foam fractionation using bovine serum albumin (BSA) as a model protein. The role of ß-cyclodextrin in preventing the aggregation of BSA induced by the gas-liquid interface was studied at the molecular level. The results indicate that by holding the exposed phenylalanine residues in its hydrophobic cavity, ß-cyclodextrin effectively prevented the aggregation of BSA induced by the gas-liquid interface in foam fractionation. Furthermore, ß-cyclodextrin could be effectively separated from BSA in the foamate due to their weak association.

A novel technology coupling extraction and foam fractionation for separating the total saponins from Achyranthes bidentata.

A novel technology coupling extraction and foam fractionation was developed for separating the total saponins from Achyranthes bidentata. In the developed technology, the powder of A. bidentata was loaded in a nylon filter cloth pocket with bore diameter of 180 µm. The pocket was fixed in the bulk liquid phase for continuously releasing saponins. Under the optimal conditions, the concentration and the extraction rate of the total saponins in the foamate by the developed technology were 73.5% and 416.2% higher than those by the traditional technology, respectively. The foamates obtained by the traditional technology and the developed technology were analyzed by ultraperformance liquid chromatography-mass spectrometry to determine their ingredients, and the results appeared that the developed technology exhibited a better performance for separating saponins than the traditional technology. The study is expected to develop a novel technology for cost effectively separating plant-derived materials with surface activity.

Separation of soybean saponins from soybean meal by a technology of foam fractionation and resin adsorption.

Foam fractionation and resin adsorption were used to recover soybean saponins from the industrial residue of soybean meal. First, a two-stage foam fractionation technology was studied for concentrating soybean saponins from the leaching liquor. Subsequently, resin adsorption was used to purify soybean saponins from the foamate in foam fractionation. The results showed that the enrichment ratio, the recovery percentage, and the purity of soybean saponins by using the two-stage foam fractionation technology could reach 4.45, 74%, and 67%, respectively. After resin adsorption and desorption, the purity of soybean saponins in the freeze-dried powder from the desorption solution was 88.4%.

Role of pH-induced structural change in protein aggregation in foam fractionation of bovine serum albumin.

For reducing protein aggregation in foam fractionation, the role of pH-induced structural change in the interface-induced protein aggregation was analyzed using bovine serum albumin (BSA) as a model protein. The results show that the decrease in pH from 7.0 to 3.0 gradually unfolded the BSA structure to increase the molecular size and the relative content of ß-sheet and thus reduced the stability of BSA in the aqueous solution. At the isoelectric point (pH 4.7), BSA suffered the lowest level in protein aggregation induced by the gas-liquid interface. In the pH range from 7.0 to 4.7, most BSA aggregates were formed in the defoaming process while in the pH range from 4.7 to 3.0, the BSA aggregates were formed at the gas-liquid interface due to the unfolded BSA structure and they further aggregated to form insoluble ones in the desorption process.

Separation of bovine serum albumin by foam fractionation with wire gauze structured packing column.

In order to enhance foam drainage, a novel foam fractionation column with the wire gauze structured packing was developed. The performances of the packing were evaluated by investigating its effects on bubble size, liquid holdup, enrichment ratio, and recovery percentage efficiency under different conditions using bovine serum albumin (BSA) aqueous solution as a model. The results showed that the packing could accelerate the bubble collapse, reduce the liquid holdup, enhance the foam drainage, and improve the BSA enrichment ratio. Specifically, the best BSA enrichment ratio was 21.78 at the liquid loading volume of 490 mL, air flow rate of 300 mL min(-1), feeding BSA concentration of 0.10 g L(-1), packing bed height of 300 mm, and feeding pH 6.2. It was 2.44 times of the BSA enrichment ratio obtained with the control column.

Technology of streptomycin sulfate separation by two-stage foam separation.

Industrial discharges from manufacturing streptomycin sulfate (SS) are inhibitory to biological wastewater treatment and need to be stripped of residual SS. For effective SS recovery from the wastewater, a two-stage foam separation technology was investigated using a column with a vertical ellipsoid-shaped channel (VEC) and a conventional one, and sodium dodecyl sulfate (SDS) served as the collector. The mechanism of enhancing foam drainage by VEC was theoretically analyzed. In the first stage, the column with VEC was used and under the optimal conditions of the liquid-loading volume 300 mL, volumetric airflow rate 100 mL/min, the initial pH 7.0 and the molar ratio of SDS to SS 8.0, an improved SS enrichment ratio of 16.7 was obtained. In the second stage, a conventional column was used and with a volumetric airflow rate of 450 mL/min, the foamate had a SS concentration of about 0.5 g/L, so it was used as the feed solution of the first stage. By the two-stage technology, the total SS recovery percentage reached as high as 99.7%. Thus, it was significantly effective for the two-stage foam separation technology to recover SS from the simulative wastewater.

Enhancing foam drainage using foam fractionation column with spiral internal for separation of sodium dodecyl sulfate.

A new type of foam fractionation column with spiral internal had been designed for enhancing the foam drainage and thus for the removal of minute hazardous materials. The column without spiral internal was served as the comparison column, and an anionic surfactant sodium dodecyl sulfate (SDS) was used as the foaming solution. Effects of liquid loading volume, initial SDS concentration and superficial gas velocity on enrichment ratio and recovery percentage of SDS were investigated. The experimental results showed that the spiral column successfully enhanced the enrichment ratio of SDS by reducing countercurrent resistance between the rising bubbles and the entrained liquid of reflux. Enrichment ratio of SDS obtained by using the spiral column was 15.7, which was 2.5 times of that obtained by using the comparison column under the suitable operating conditions of liquid loading volume 0.4 L, initial SDS concentration 0.2g/L and superficial gas velocity 1.7 mm/s.

Removal of color from textile dyeing wastewater by foam separation.

The feasibility of foam separation for color removal from direct dyes-containing wastewater was assessed using actual textile wastewater as the research system and cetyl trimethyl ammonium bromide (CTAB) as the collector. The influences of liquid loading volume, air flow rate, surfactant concentration, and initial pH on the removal efficiency and reuse of CTAB in the foamate were studied. The results indicated that using CTAB as a collector for foam separation can provide good foaming quality and effectively remove color from textile wastewater. Under optimum operational conditions (liquid loading volume 450 mL, gas flow rate of 500 mL/min, CTAB concentration 20 mg/L, and an initial pH of 7.0), the removal efficiency reached 88.9%. The residual dye content met the discharge standard for the dyeing and finishing textile industry (GB4287-92) published by the Ministry of Environmental Protection of the People's Republic of China. Using recycled foamate in untreated wastewater, the removal efficiency of 87.5% was obtained with CTAB concentration 10 mg/L of the wastewater.

Effects of pH profiles on nisin fermentation coupling with foam separation.

Online foam separation was proposed to recover nisin during fermentation of Lactococcus lactis subsp. lactis ATCC 11454. Firstly, the optimal pH profile of nisin fermentation was investigated including different realkalization set values and pH drop gradients. Then the selected pH profiles of 5.75+/-0.05 and 6.25-5.75 (+/-0.02) were used to perform nisin fermentation coupling with foam separation. The results showed that pH profile of 5.75+/-0.05 was better than that of 6.25-05.75 (+/-0.02) for online foam separation. With the optimal pH profile, an aeration of 20 ml min(-1) that started at 8 h of incubation and lasted for 2 h resulted in 6.6 times higher specific productivity than that of the fermentation without aeration. Nisin synthesis was therefore prolonged with low sucrose concentration in the culture broth, which indicated that the feedback inhibition of nisin is more influential than the substrate limitation of sucrose in the late phase of nisin fermentation. Total nisin production (4,870+/-180 IU ml(-1)) was increased by 30.3% with online foam separation. This effective online recovery method for nisin production could be easily scaled up due to the facile operation of foaming process.