BRD9 status is a major contributor for cysteine metabolic remodeling through MST and EAAT3 modulation in malignant melanoma.

Cutaneous melanoma (CM) is the most aggressive skin cancer, showing globally increasing incidence. Hereditary CM accounts for a significant percentage (5-15 %) of all CM cases. However, most familial cases remain without a known genetic cause. Even though, BRD9 has been associated to CM as a susceptibility gene. The molecular events following BRD9 mutagenesis are still not completely understood. In this study, we disclosed BRD9 as a key regulator in cysteine metabolism and associated altered BRD9 to increased cell proliferation, migration and invasiveness, as well as to altered melanin levels, inducing higher susceptibility to melanomagenesis. It is evident that BRD9 WT and mutated BRD9 (c.183G>C) have a different impact on cysteine metabolism, respectively by inhibiting and activating MPST expression in the metastatic A375 cell line. The effect of the mutated BRD9 variant was more evident in A375 cells than in the less invasive WM115 line. Our data point out novel molecular and metabolic mechanisms dependent on BRD9 status that potentially account for the increased risk of developing CM and enhancing CM aggressiveness. Moreover, our findings emphasize the role of cysteine metabolism remodeling in melanoma progression and open new queues to follow to explore the role of BRD9 as a melanoma susceptibility or cancer-related gene.

Comparative analysis of cancellous graft and cortico-cancellous graft in osteosynthesis of scaphoid pseudoarthrosis with plate.

INTRODUCTION: Scaphoid is the most fractured carpal bone, with a 5-10% nonunion rate. Treatment challenges include choosing the implant and graft that best corrects humpback deformity and carpal malalignment with higher chances of bony healing. OBJECTIVE: Compare cortico-cancellous and cancellous grafts between two groups of patients treating scaphoid nonunion with locking plates using autologous bone graft and evaluate bone healing rates and radiographic, tomographic, and functional parameters before and after surgery. METHODS: Non-randomized prospective study including 20 cases of scaphoid nonunion. Groups were divided into Group A (ten patients treated with cortico-cancellous iliac graft) and Group B (ten patients treated with cancellous iliac graft). Patients underwent pre- and postoperative radiographs, computed tomography, and functional evaluation. RESULTS: In postoperative analysis, Group A showed a statistically significant difference in intrascaphoid angle (p = 0.002) and scapholunate angle (p = 0.011) correction when comparing the pre- and postoperative periods. Group B showed a statistically significant difference in intrascaphoid angle (p = 0.002) and scapholunate angle correction (p = 0.0018), grip strength (p = 0.002), and tip pinch strength (p = 0.001) when comparing the pre- and postoperative periods. By comparing both groups, Group B showed a statistically significant difference in intrascaphoid angle correction (p = 0.002), grip strength (p = 0.002), tip pinch strength (p = 0.002), and radial deviation (p = 0.0003). There was no statistical difference when comparing bony healing between groups. CONCLUSION: Scaphoid nonunion treatment with a locking plate was effective, showing a high bony healing rate and improved carpal alignment in imaging tests for both graft types. However, results for intrascaphoid angle correction, grip strength, tip pinch strength, and radial deviation were better in Group B. LEVEL OF EVIDENCE: IV, case series.

Comparative analysis of scaphoid nonunion treatment with screw fixation and angular stable plate.

INTRODUCTION: Scaphoid fractures represent 5-10% of nonunion rate and the treatment options consist of an open reduction with correction of deformity, restoration of the scaphoid length with autologous wedge grafting and fixation. However, there is still no consensus in the literature on the best fixation method. Therefore, the purpose of the study is to compare plate fixation and screw fixation in treating scaphoid nonunion with humpback deformity and carpal instability. METHODS: Prospective, non-randomised study comparing the treatment of two groups of patients with scaphoid nonunion. A total of 19 patients were included in the study, the first ten patients were included in group 1 (plate fixation), subsequently nine patients were included in group 2 (screw fixation). The nonunion duration was longer than 6 months and patients did not present type III Scaphoid Nonunion Advanced Collapse (SNAC). Clinical evaluations included pain intensity, range of motion, grip strength, pinch test and functional scales Disabilities of the Arm, Shoulder and Hand (DASH) and Mayo Wrist Score. Radiographic evaluations consisted of radiographs of both wrists in AP, AP with ulnar deviation, lateral and oblique views. Patients further underwent a tomography of the affected wrist for bone deformity, carpal collapse and later consolidation evaluation. RESULTS: According to post-operative measurements, group 1 showed a significant improvement in the scapholunate angle (p = 0.011) and in the intrascaphoid angle (p = 0.002). Group 2 only showed an improvement in the scapholunate angle (p = 0.011). All patients in group 1 achieved consolidation in 8 weeks, while group 2 patients had a slower consolidation, with a mean of 14 weeks and standard deviation (SD) of 4.2, with statistical significance (p = 0.006). CONCLUSION: Our prospective study, despite the limitations, contributes to the literature for demonstrating a better fixation using plate, with a better correction of humpback deformity and Dorsal Intercalated Segment Instability (DISI) and a faster consolidation. LEVEL OF EVIDENCE: II, therapeutics studies; prospective comparative study.

PROFILE OF PATIENTS WITH OSTEOPOROTIC FRACTURES AND FACTORS THAT DECREASE PREVENTION.

OBJECTIVE: To evaluate the epidemiological profile of patients with osteoporotic fractures compared to patients with osteoarthritis (OA) and identify factors that diminish adherence to secondary prevention. METHODS: A total of 108 patients with osteoporotic fractures (OF) were compared to 86 patients with OA. RESULTS: Patients in the OF group were older (p < 0.001); had a lower body mass index (p < 0.001); were less literate (p = 0.012); were more frequently Caucasian (p = 0.003); were less frequently married (p < 0.001); experienced more falls, cognitive deficiency, previous fractures, old fracture, falls in the last year, and fall fractures; needed more help and took more medicine for osteoporosis (p < 0.05); and showed less pathology in the feet, muscle weakness, less vitamin D intake, and lower Katz & Lawton scores (p < 0.001). Factors that increased the chance of nonadherence included older age (p = 0.020), falls (p = 0.035), cognitive deficiency (p = 0.044), and presence of depression/apathy/confusion (p < 0.001). CONCLUSION: Patient age, ethnicity, marital status, previous falls, foot pathologies, muscle weakness, previous fractures, use of vitamin D, use of osteoporosis drugs, and lower Katz & Lawton scale score defined the OF group. Factors that increased the chance of nonadherence included older age, sedative use, cognitive disorders, and symptoms of depression/apathy/confusion. Level of Evidence III, Case-control.

OBJETIVO: Avaliar o perfil epidemiológico de pacientes com fraturas osteoporóticas, comparando com pacientes com osteoartrite (OA) e identificar fatores que diminuam aderência à prevenção secundária. MÉTODOS: 108 pacientes com FO foram comparados a 86 pacientes com OA. RESULTADOS: Grupo FO era mais velho (p< 0,001), com menor IMC (p<0,001), menos alfabetizado (p = 0,012), com maior frequência de brancos (p = 0,003), menor frequência de casados (p< 0,001). Apresentaram mais quedas, deficiência cognitiva, fraturas prévias, fratura antiga, queda no último ano, fraturas por queda. Necessitam de mais auxílio e tomam mais medicamento para osteoporose (p< 0,05); apresentaram menos patologia nos pés, fraqueza muscular. Tomam menos vitamina D e menor Katz & Lawton (p<0,001). Tem aumento da chance de não aderência: maior idade (p = 0,020), sedativo (p = 0,020), quedas (p = 0,035), deficiência cognitiva (p = 0,044) e presença de depressão/apatia/confusão (p< 0,001). CONCLUSÃO: Idade do paciente, etnia, estado civil, quedas prévias, patologias nos pés, fraqueza muscular, fraturas prévias, uso de vitamina D, uso de medicamentos para osteoporose e a escala Katz & Lawton definem o grupo FO. Aumentam a chance de não aderência: maior idade, sedativos, distúrbios cognitivos e sintomas de depressão/apatia/confusão. Nível de Evidência III, Estudo de caso controle.

Profile of patients with osteoporotic fractures and factors that decrease prevention / Perfil de pacientes com fraturas osteoporóticas e fatoresque diminuem aderência à prevenção

ABSTRACT Objective: To evaluate the epidemiological profile of patients with osteoporotic fractures compared to patients with osteoarthritis (OA) and identify factors that diminish adherence to secondary prevention. Methods: A total of 108 patients with osteoporotic fractures (OF) were compared to 86 patients with OA. Results: Patients in the OF group were older (p < 0.001); had a lower body mass index (p < 0.001); were less literate (p = 0.012); were more frequently Caucasian (p = 0.003); were less frequently married (p < 0.001); experienced more falls, cognitive deficiency, previous fractures, old fracture, falls in the last year, and fall fractures; needed more help and took more medicine for osteoporosis (p < 0.05); and showed less pathology in the feet, muscle weakness, less vitamin D intake, and lower Katz & Lawton scores (p < 0.001). Factors that increased the chance of nonadherence included older age (p = 0.020), falls (p = 0.035), cognitive deficiency (p = 0.044), and presence of depression/apathy/confusion (p < 0.001). Conclusion: Patient age, ethnicity, marital status, previous falls, foot pathologies, muscle weakness, previous fractures, use of vitamin D, use of osteoporosis drugs, and lower Katz & Lawton scale score defined the OF group. Factors that increased the chance of nonadherence included older age, sedative use, cognitive disorders, and symptoms of depression/apathy/confusion. Level of Evidence III, Case-control.

RESUMO Objetivo: Avaliar o perfil epidemiológico de pacientes com fraturas osteoporóticas, comparando com pacientes com osteoartrite (OA) e identificar fatores que diminuam aderência à prevenção secundária. Métodos: 108 pacientes com FO foram comparados a 86 pacientes com OA. Resultados: Grupo FO era mais velho (p< 0,001), com menor IMC (p<0,001), menos alfabetizado (p = 0,012), com maior frequência de brancos (p = 0,003), menor frequência de casados (p< 0,001). Apresentaram mais quedas, deficiência cognitiva, fraturas prévias, fratura antiga, queda no último ano, fraturas por queda. Necessitam de mais auxílio e tomam mais medicamento para osteoporose (p< 0,05); apresentaram menos patologia nos pés, fraqueza muscular. Tomam menos vitamina D e menor Katz & Lawton (p<0,001). Tem aumento da chance de não aderência: maior idade (p = 0,020), sedativo (p = 0,020), quedas (p = 0,035), deficiência cognitiva (p = 0,044) e presença de depressão/apatia/confusão (p< 0,001). Conclusão: Idade do paciente, etnia, estado civil, quedas prévias, patologias nos pés, fraqueza muscular, fraturas prévias, uso de vitamina D, uso de medicamentos para osteoporose e a escala Katz & Lawton definem o grupo FO. Aumentam a chance de não aderência: maior idade, sedativos, distúrbios cognitivos e sintomas de depressão/apatia/confusão. Nível de Evidência III, Estudo de caso controle.

Ortopedia e traumatologia: princípios e prática / Orthopedics and traumatology: principles and practice

Esta nova edição de Ortopedia e traumatologia: princípios e prática reúne mais de 170 autores, que apresentam o que há de mais atual em termos de técnicas de diagnóstico e tratamento, mantendo a abordagem ampla e acessível, característica das edições anteriores. Expandido e com capítulos totalmente remodelados e atualizados, este livro é enriquecido com mais de 2.500 ilustrações que auxiliam no entendimento dos tópicos abordados. Dentre os novos temas, encontram-se as deformidades paralíticas e não-paralíticas da coluna toracolombar; a reabilitação funcional da coluna vertebral: a reabilitação das lesões não-traumáticas do membro superior; a reabilitação funcional do quadril da criança; a artroscopia de punho, do quadril e do tornozelo; a revisão de artroplastia total do joelho; os tumores ósseos benignos e as lesões pseudotumorais;. Os tumores ósseos malignos e as lesões metastáticas; bem como as lesões dos nervos periféricos. Outra novidade desta edição é o CD-ROM que acompanha o livro, incluindo vídeos de procedimentos artroscópicos (que complementam os capítulos específicos sobre a técnica), acompanhamentos de casos (que ilustram os capítulos sobre marcha normal e patológica e defeitos de fechamento do tubo neural) e os arquivos pdf dos três capítulos da seção sobre vias de acesso da edição anterior (esses capítulos foram reproduzidos no CD-ROM, conforme publicados na terceira edição do livro, para aqueles leitores que se interessarem pelo assunto). Além disso, os vídeos sobre exame físico, que na terceira edição acompanhavam o livro em CD-rom, estão agora disponíveis para acesso via Internet, no link deste livro no site da editora (www.artmed.com.br). Estes materiais, bem como outros, sejam atualizações ou complementos, também poderão ser acessados diretamente no hot site do livro (www.artmed.com.br/ortopediaetraumatologia)