Association of Bone Turnover Markers with Type 2 Diabetes Mellitus and Microvascular Complications: A Matched Case-Control Study.

Purpose: The aim of this study was to evaluate the association of bone turnover markers (BTMs) with type 2 diabetes mellitus (T2DM) and microvascular complications. Methods: A total of 166 T2DM patients and 166 non-diabetic controls matched by gender and age were enrolled. T2DM patients were sub-classified into groups based on whether they had diabetic peripheral neuropathy (DPN), diabetic retinopathy (DR), and diabetic kidney disease (DKD). Clinical data including demographic characteristics and blood test results [serum levels of osteocalcin (OC), N-terminal propeptide of type 1 procollagen (P1NP), and ß-crosslaps (ß-CTX)] were collected. Logistic regression and restrictive cubic spline curves were performed to examine the association of BTMs with the risk of T2DM and microvascular complications. Results: After adjusting for family history of diabetes, sex and age, an inverse association was observed between elevated serum OC levels [O, p < 0.001] and increased serum P1NP levels , p < 0.001] with the risk of T2DM. Moreover, there was an inverse linear association of serum OC and P1NP levels with the risk of T2DM. However, ß-CTX was not associated with T2DM. Further analysis showed a nonlinear association between OC and the risk of DR, while P1NP and ß-CTX were not correlated with DR. Serum concentrations of BTMs were not associated with the risks of DPN and DKD. Conclusion: Serum OC and P1NP levels were negatively correlated with T2DM risk. Particularly, serum OC levels were associated with DR risk. Given that BTMs are widely used as markers of bone remodeling, the present finding provides a new perspective for estimating the risk of diabetic microvascular complications.

A dose-response meta-analysis between serum concentration of 25-hydroxy vitamin D and risk of type 1 diabetes mellitus.

It remains debatable whether vitamin D plays any role as a risk factor for type 1 diabetes mellitus (T1DM). We have summarized the effect of circulating 25-hydroxy vitamin D [25(OH)D] concentration on the risk of developing T1DM via a dose-response meta-analysis. We undertook a database search on PubMed, Embase, and Cochrane Library from inception to January 2020. A meta-analysis based on random-effects model was applied. Subgroup analysis and meta-regression were performed to inspect the source of heterogeneity. Dose-response data were examined using the generalized least squares trend estimation method. This study was registered with the PROSPERO (ID: CRD42020166174). In total, 16 studies including 10,605 participants (3913 case patients) were included. The pooled odds ratios (OR) and 95% confidence intervals (95% CI) for the highest versus the lowest 25(OH)D concentration was 0.39 (0.27, 0.57), with a high heterogeneity (I2 = 76.7%, P < 0.001). Meta-regression analysis identified latitude (P = 0.02), adjustment for gender (P = 0.001), and 25(OH)D stratification (P < 0.001) as sources of heterogeneity. Furthermore, the nonlinear dose-response analysis determined the OR (95% CI) of T1DM to be 0.91 (0.90, 0.93) per 10 nmol/L increase in the 25(OH)D concentration. A 'U'-shaped association was found between serum 25(OH)D concentration and risk of T1DM. The present study highlights the significant inverse association between the circulating 25(OH)D concentration and the risk of T1DM.

Cationic Polymers with Tailored Structures for Rendering Polysaccharide-Based Materials Antimicrobial: An Overview.

Antimicrobial polymers have attracted substantial interest due to high demands on improving the health of human beings via reducing the infection caused by various bacteria. The review presented herein focuses on rendering polysaccharides, mainly cellulosic-based materials and starch to some extent, antimicrobial via incorporating cationic polymers, guanidine-based types in particular. Extensive review on synthetic antimicrobial materials or plastic/textile has been given in the past. However, few review reports have been presented on antimicrobial polysaccharide, cellulosic-based materials, or paper packaging, especially. The current review fills the gap between synthetic materials and natural polysaccharides (cellulose, starch, and cyclodextrin) as substrates or functional additives for different applications. Among various antimicrobial polymers, particular attention in this review is paid to guanidine-based polymers and their derivatives, including copolymers, star polymer, and nanoparticles with core-shell structures. The review has also been extended to gemini surfactants and polymers. Cationic polymers with tailored structures can be incorporated into various products via surface grafting, wet-end addition, blending, or reactive extrusion, effectively addressing the dilemma of improving substrate properties and bacterial growth. Moreover, the pre-commercial trial conducted successfully for making antimicrobial paper packaging has also been addressed.

Immobilizing Laccase on Different Species Wood Biochar to Remove the Chlorinated Biphenyl in Wastewater.

Biochars produced from two different wood species over a microwave assisted pyrolysis process were used as novel and green-based supports for immobilizing enzyme, laccase in particular. The results obtained from FT-IR, SEM and BET measurements indicated that Maple biochar with honeycomb structure has higher surface area and pore volume than Spruce biochar; and there exist O-H, C-H, C=O and C=C groups in biochars for potential chemical modification. The best laccase immobilization conditions identified from an orthogonal experiment were pH = 3, laccase concentration 16 g/L and contact time 8 h. Under such conditions, the high immobilization yield (64.2%) and amount (11.14 mg/g) of laccase on Maple biochar were achieved, leading to the significantly improved thermal stability of laccase. Moreover, the immobilized laccase is reusable and enhanced the enzymatic degradation of 4-hydroxy-3,5-dichlorobiphenyl (71.4% yield), thus creating a promising and novel type of adsorbent in the removal of polychlorinated biphenyls from wastewater.

Microrheology, advances in methods and insights.

Microrheology is an emerging technique that probes mechanical response of soft material at micro-scale. Generally, microrheology technique can be divided into active and passive versions. During last two decades, extensive efforts have been paid to improve both the experiment techniques and data analysis methods, especially about how to link consequential particle positions into trajectories. We review the recent advances in microrheology, including improvements in labeling, imaging, data acquiring, data processing and data interpretation. Some of the recent insights in soft matter and living systems gained by using this technique are given. Before these, we also give a very brief description of the basic principles of both active and passive microrheology techniques, and some details about optical particle tracking and DWS.

Hydrogen bonding energy determined by molecular dynamics simulation and correlation to properties of thermoplastic starch films.

The molecular dynamics (MD) simulation method was used to investigate the hydrogen bonding energy of starch/glycerol system under different temperatures (range from 90°C to 120°C) and different glycerol contents (range from 20% to 40%, based on dry starch weight). These effects on the hydrogen bonding energy (including the total hydrogen bonding energy, hydrogen bonding energy of starch/starch, glycerol/glycerol, and starch/glycerol) were analyzed in detail. Meanwhile, glycerol plasticized starch films were prepared using casting method. The relationship between the hydrogen bonding energy and the performances of thermoplastic starch film (TPSF), such as crystallinity, mechanical properties and water uptake determined experimentally, were revealed and discussed. The results indicated that glycerol/starch film contained strong hydrogen bonding interaction which could be increased by decreasing the temperature or increasing the glycerol content. The hydrogen bonding interaction is the key factor for the preparation of the plasticized starch material, and the plasticized mechanism can be interpreted according to the analytical results of the simulation.

Comment on "An alternative theory to explain the effects of coalescing oil drops on mouthfeel" by B. Le Reverend and J. Engmann, Soft Matter, 2015, 11, 7077.

In a recent paper by B. Le Reverend and J. Engmann, they used a model to explain the change in the perceived viscosity by phase separation. We improved this model by adding the drop in viscosity in the aqueous phase to it and we show how this will significantly change the conclusion in the original paper. The increase in viscosity due to phase separation is highly unlikely to happen because the drop in viscosity due to loss of oil is faster at a high oil concentration.

Probing Conformational Change of Bovine Serum Albumin-Dextran Conjugates under Controlled Dry Heating.

The time-dependent conformational change of bovine serum album (BSA) during Maillard reaction with dextran under controlled dry heating has been studied by small-angle X-ray scattering, fluorescence spectroscopy, dynamic light scattering, and circular dichroism analysis. Through the research on the radii of gyration (Rg), intrinsic fluorescence, and secondary structure, conjugates with dextran coating were found to inhibit BSA aggregation and preserve the secondary structure of native BSA against long-time heat treatment during Maillard reaction. The results suggested that the hydrophilic dextran was conjugated to the compact protein surface and enclosed it and more dextran chains were attached to BSA with the increase of the heating time. The study presented here will be beneficial to the understanding of the conformational evolution of BSA molecules during the dry-heating Maillard reaction and to the control of the protein-polysaccharide conjugate structure.

Structure, morphology, and assembly behavior of kafirin.

Prolamins from grains have attracted intensive attention in recent years due to their potential in satisfying the demand for environmentally friendly (biodegradable), abundantly available (sustainable), and cost-effective biomaterials. However, for kafirin, the prolamin from sorghum, its composition, structure, morphology, and self-assembly behaviors have not been fully characterized. In this paper, kafirin was extracted from the whole sorghum grain and found to contain 68, 14, 6, and 12% of α-, ß-, and γ-fractions and cross-linked kafirin, respectively. Freeze-dried kafirin contained ∼49% α-helix in the solid state. When dissolved in 65% (v/v) isopropanol, 60% (v/v) tert-butanol, and 85% (v/v) ethanol aqueous solvents, the relative α-helix content in kafirin increased with the decrease of solvent polarity. Structural analysis using small-angle X-ray scattering (SAXS) indicated that kafirin (2 mg/mL) took stretched and extended conformations with dimensions of 118 × 15 × 15 and 100 × 11 × 11 Å in 60% tert-butanol and 65% isopropanol, respectively. More elongated conformation of individual kafirin with high-order assembly was observed in 85% ethanol. Protein aggregation occurred as protein concentration increased in its good solvent. The morphology of kafirin assemblies captured by atomic force microscopy (AFM) revealed that kafirin protein took uniform particle morphology at low concentration, and disk-like or rod-like structures resulting from solvent evaporation induced particle interactions emerged at high concentrations. These results suggest that both protein concentration and solvent polarity can effectively regulate kafirin assemblies from thick rod-like to slim rod-like structures, a convenient way to tune the fibrillation of prolamin-based biomaterials.

Understanding the dissolution of α-zein in aqueous ethanol and acetic acid solutions.

Zein is a corn prolamin that has broad industrial applications because of its unique physical properties. Currently, the high cost of extraction and purification, which is directly related to the dispersion of zein in different solvents, is the major bottleneck of the zein industry. Solution behaviors of zein have been studied for a long time. However, the physical nature of zein in different solvents remains unclear. In this study, small-angle X-ray scattering (SAXS), static light scattering (SLS), and rheology were combined to study the structure and protein-solvent interaction of α-zein in both acetic acid and aqueous ethanol solutions. We found that the like-dissolve-like rule, the partial unfolding, and the protonation of zein are all critical to understanding the solution behaviors. Zein holds an elongated conformation (i.e., prolate ellipsoid) in all solutions, as revealed from SAXS data. There is an "aging effect" for zein in aqueous ethanol solutions, as evidenced by the transition of Newtonian rheological profiles for fresh zein solutions to the non-Newtonian shear thinning behavior for zein solutions after storage at room temperature for 24 h. Such shear thinning behavior becomes more pronounced for zein solutions at higher concentrations. The SLS results clearly show that acetic acid is a better solvent to dissolve zein than aqueous ethanol solution, as supported by a more negative second virial coefficient. This is majorly caused by the protonation of the protein, which was further verified by the dissolution of zein in water (a nonsolvent for zein) with the addition of acids.

Scaling behaviors of α-zein in acetic acid solutions.

Whether the concentration scaling behavior of a protein solution is similar to that of neutral polymer solutions, polyelectrolyte solutions, or neither still remains unclear. In this paper, the structure and rheological properties of α-zein in acetic acid solutions have been investigated by small-angle X-ray scattering (SAXS), rheology, and circular dichroism (CD) measurements. Through the investigation of the radii of gyration, the secondary structure, and the solution viscosities of α-zein in acetic acid solutions as a function of α-zein concentration, we observed two distinct scaling regions with an identical threshold. This critical concentration is close to the bulk density of the α-zein in very dilute solution. The scaling relationships are close to the theoretical predictions for polyelectrolyte solutions, but obvious discrepancies still exist. The phenomena presented here may be widely present in other protein solutions, which can stimulate more attentions on the understanding of the scaling behaviors of protein solutions.