Laparoscopic radical resection for rectal cancer in a patient with uncorrected truncus arteriosus type IV: A case report.

Persistent truncus arteriosus is a rare congenital heart malformation which if not corrected, results in the death of about 50% of the patients, while fewer than 20% of the patients survive the first year of life. Here, we report the successful anesthetic management of an adult patient with uncorrected truncus arteriosus who presented for the laparoscopic radical resection of rectal cancer.

Resección radical laparoscópica para el cáncer de recto en un paciente con tronco arterioso no corregido tipoIV: informe de un caso / Laparoscopic radical resection for rectal cancer in a patient with uncorrected truncus arteriosus typeIV: A case report

El tronco arterioso persistente es una malformación cardíaca congénita poco frecuente. Si no se corrige, aproximadamente el 50% de los que lo padecen mueren; menos del 20% de estos pacientes sobreviven al primer año de vida. Presentamos el manejo anestésico exitoso de un paciente adulto con tronco arterioso no corregido que se sometió a resección radical laparoscópica de cáncer de recto.(AU)

Persistent truncus arteriosus is a rare congenital heart malformation which if not corrected, results in the death of about 50% of the patients, while fewer than 20% of the patients survive the first year of life. Here, we report the successful anesthetic management of an adult patient with uncorrected truncus arteriosus who presented for the laparoscopic radical resection of rectal cancer.(AU)

Spin Excitation Spectra of Anisotropic Spin-1/2 Triangular Lattice Heisenberg Antiferromagnets.

Investigation of dynamical excitations is difficult but crucial to the understanding of many exotic quantum phenomena discovered in quantum materials. This is particularly true for highly frustrated quantum antiferromagnets whose dynamical properties deviate strongly from theoretical predictions made based on the spin-wave or other approximations. Here, we present a large-scale numerical calculation on the dynamical correlation functions of spin-1/2 triangular Heisenberg model using a state-of-the-art tensor network renormalization group method. The calculated results allow us to gain for the first time a comprehensive picture on the nature of spin excitation spectra in this highly frustrated quantum system. It provides a quantitative account for all the key features of the dynamical spectra disclosed by inelastic neutron scattering measurements for Ba_{3}CoSb_{2}O_{9}, revealing the importance of the interplay between low- and high-energy excitations and its renormalization effect to the low-energy magnon bands and high-energy continuums. We identify the longitudinal Higgs modes in the intermediate-energy scale and predict the energy and momentum dependence of spectral functions along the three principal axes that can be verified by polarized neutron scattering experiments. Furthermore, we find that the spin excitation spectra weakly depend on the anisotropic ratio of the antiferromagnetic interaction.

Characterization of genetic fundamentals for piglet mortality at birth in Yorkshire, Landrace, and Duroc sows.

Piglet mortality is an economically important complex trait that impacts sow prolificacy. Genetic analyses for piglet mortality at weaning have been reported in dozens of studies, but not for piglet mortality at birth. In this study, we used multi-breed data sets from Yorkshire, Landrace, and Duroc sows to characterize the genetic fundamentals of piglet mortality at birth. The heritabilities from parity I to III were estimated to be 0.0630, 0.1031, and 0.1140 respectively. By using a combined strategy, a total of 21 SNPs were detected in three parities, of which six were observed in parity I, five in parity II and 10 in parity III. Genome annotation revealed that these SNPs were harbored within or close to 19 candidate genes. The candidate genes were found to associate with the reproductive system and embryonic development in the tissue expression database, which are reasonably related to piglet mortality. These findings are expected to provide much information for understanding the genetic and genomic fundamentals of farrowing mortality.

[An investigation and analysis on a poisoning tetramine accident].

From August 21 to December 13, 2018, a tetramine poisoning incident in Wenzhou, Zhejiang Province was investigated, and the clinical diagnosis and treatment of tetramine poisoning was analyzed. There were 6 cases of poisoning caused by artificial tetramine poisoning. The diagnosis was delayed, coma and convulsions were severe manifestations continuous renal replacement therapy (CRRT) was effective in the treatment of severe cases, and all 6 cases were cured. The possibility of poisoning should be considered for unexplained coma and/or convulsions. Although tetramine is banned, it still needs to be highly vigilant and avoids the recurrence of delayed diagnosis and treatment.

Critical properties of the two-dimensional q-state clock model.

We perform the state-of-the-art tensor network simulations directly in the thermodynamic limit to clarify the critical properties of the q-state clock model on the square lattice. We determine accurately the two phase transition temperatures through the singularity of the classical analog of the entanglement entropy, and provide extensive numerical evidences to show that both transitions are of the Berezinskii-Kosterlitz-Thouless (BKT) type for q≥5 and that the low-energy physics of this model is well described by the Z_{q}-deformed sine-Gordon theory. We also determine the characteristic conformal parameters, especially the compactification radius, that govern the critical properties of the intermediate BKT phase.

Reduced long non-coding RNA PTENP1 contributed to proliferation and invasion via miR-19b/MTUS1 axis in patients with cervical cancer.

OBJECTIVE: Many studies showed that long non-coding RNAs (lncRNAs) may serve as prospective markers for patients with malignant cancers, including cervical cancer (CC). In this study, we mainly investigate the functions of lncRNA PTENP1 in the progression of human CC. MATERIALS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect expression levels of PTENP1, miR-19b and MTUS1 in CC tissues, the adjacent tissues and CC cell lines. The correlations between PTENP1 with miR-19b, miR-19b with MTUS1 and PTENP1 with MTUS1 were analyzed. Overall survival (OS) of patients was analyzed using Kaplan-Meier method. Proliferation capacity was measured by CCK-8 assay and the invasion ability in CC cell line was detected by transwell assay. Western blot (WB) assay was performed to measure protein levels of tissues and CC cell lines. Finally, Dual-Luciferase reporter assay was performed to prove the potential binding sites between PTENP1 and miR-19b, miR-19b and MTUS1. RESULTS: We found that PTENP1 was reduced in CC tissues and CC cell lines, which predicted the poor diagnosis of CC patients. MiR-19b was increased in CC tissues, which was negatively correlated with PTENP1 in CC tissues. MTUS1 was reduced in CC tissues, which was negatively correlated with miR-19b and positively correlated within PTENP1 CC tissues. Furthermore, PTENP1 overexpression inhibited cell proliferation ability and invasion capacity in HeLa cells, as well as repressed expressions of Cyclin D1, N-cadherin, and Vimentin. Moreover, Luciferase gene reporter assays verified that miR-19b was a direct target miRNA of PTENP1, and MTUS1 was identified as a direct target of miR-19b. In addition, the inhibited cell proliferation and invasion abilities in HeLa cells with p-PTENP1 were eliminated following with miR-19b mimic transfection. CONCLUSIONS: According to the results, this study showed that PTENP1 was reduced in CC patients and it was a prognostic factor for CC patients. Furthermore, we firstly uncovered that PTENP1 could inhibit cell proliferation and invasion via miR-19b/MTUS1 in CC patients, which uncovered the tumor-suppressive role of PTENP1 in CC and suggested that it might be a potential target for treating human CC.

[Advances in the treatment of tinnitus].

Tinnitus is the perception of sound without an external source. Its impact is wide-ranging and heterogeneous across individuals. Since tinnitus is a subjective sensation, the treatment of tinnitus is still in the research and exploration stage at present. This study summarized the common treatments of tinnitus and their research progress to provide clinicians with references for selecting the appropriate treatments for the tinnitus sufferers.

Technical note: Genomic evaluation for crossbred performance in a single-step approach with metafounders.

A single-step genomic BLUP method (ssGBLUP) has been successfully developed and applied for purebred and crossbred performance in pigs. However, it requires phasing the genotypes and inferring the breed origin of alleles in crossbred animals, which is somewhat inconvenient. Recently, a new concept of metafounders that considers the relationship within and across base populations was developed. With this concept of metafounders, regular methods to build and invert the pedigree relationships matrix can be used with only minor modifications and, moreover, genomic relationships and pedigree-based relationships are automatically compatible in the ssGBLUP. In this study, data for the total number of piglets born in Danish Landrace, Yorkshire, and 2-way crossbred pigs and models for purebred and crossbred performance were revisited by use of ssGBLUP with 2 metafounders. Genetic variances and genetic correlations between purebred and crossbred performances were first reestimated. Then, model-based reliabilities of purebred boars for their crossbred performance and predictive abilities for crossbred animals were compared in different scenarios. Results in this study were compared to those in a previous study with identical data but with models that required known breed origin of crossbred genotypes. Results show that relationships for base individuals within Landrace and within Yorkshire are similar and that the ancestor populations for Landrace and Yorkshire are related. In terms of model-based reliabilities and predictive abilities, ssGBLUP with metafounders performs at least as well as the single-step method requiring phasing at a lower complexity.

Gapless Spin-Liquid Ground State in the S=1/2 Kagome Antiferromagnet.

The defining problem in frustrated quantum magnetism, the ground state of the nearest-neighbor S=1/2 antiferromagnetic Heisenberg model on the kagome lattice, has defied all theoretical and numerical methods employed to date. We apply the formalism of tensor-network states, specifically the method of projected entangled simplex states, which combines infinite system size with a correct accounting for multipartite entanglement. By studying the ground-state energy, the finite magnetic order appearing at finite tensor bond dimensions, and the effects of a next-nearest-neighbor coupling, we demonstrate that the ground state is a gapless spin liquid. We discuss the comparison with other numerical studies and the physical interpretation of this result.

[Icariin reduces S-nitrosogultathione induced endothelial cell apoptosis through modulating AKT/P53 pathway].

OBJECTIVE: To observe the effects of icariin on S-nitrosogultathione(GSNO) induced endothelial cell apoptosis, and to explore the relative mechanisms. METHODS: EA.hy926 cell line was provided by Zhejiang University and cells were divided into blank control group, GSNO group (1 mmol/L GSNO), icariin (ICA) intervention group (GSNO+ different concentrations (high, medium and low: 10, 1 and 0.1 µmol/L ICA) and 1 µmol/L LY294002 pretreatment groups (AKT protease pathway inhibitor on top of ICA groups) by cluster random sample method. After 48 hours. EA.hy 926 cell survival was detected by thiazolyl blue tetrazolium bromicle (MTT) method. Lactate dehydrogenase (LDH) activity, malonaldehyde (MDA) content, reactive oxygen species (ROS) level, mitochondrial membrane potential were also measured. The protein expression of protein kinase B(AKT)/phosphorylation protein kinase B(p-AKT), people tumor-suppressor protein (protein 53, P53), cytochrome C (CYC), endothelial nitric oxide synthetase (eNOS)/phosphorylation endothelial nitric oxide synthetase (p-eNOS), procaspase-3/caspase-3 was detected by Western blot. RESULTS: (1) The cell survival rate was significantly lower in GSNO group than in the blank control group (P< 0.01), which was significantly higher in the high, medium and low concentration ICA groups than in the GSNO group (all P< 0.01). (2) The LDH activity was significantly higher in the GSNO group than in the blank control group ((142.65±5.56) U/L vs. (50.01±3.42) U/L, P< 0.05), which was significantly reduced by high, medium and low concentration ICA ((98.02±3.52), (105.29±6.89) and (117.16±4.27) U/L vs. (142.65±5.56) U/L, all P< 0.05) in a dose-dependent manner. (3) The MDA content was significantly higher in GSNO group than in the blank control group ((11.14±0.37) nmol/mg vs. (5.21±0.18) nmol/mg, P< 0.05), which could be reduced by pretreatment with high, medium and low concentration ICA ((6.60±0.41), (6.83±0.21) and (8.29±0.07) nmol/mg vs. (11.14 ±0.37) nmol/mg, all P< 0.05). (4) The ROS content was significantly higher in GSNO group than in the blank control group ((173.15±11.12)% (relative ratio to the blank control group), P< 0.05), which could be significantly reduced by pretreatment with high, medium and low concentration ICA ((122.56±8.09)%, (134.52±9.09)%, and(149.89±9.16)% (the ratio of the above are compared with the blank control group), P< 0.05). (5) The mitochondrial membrane potential was significantly higher in the GSNO group (0.84 ± 0.04) than in the blank control group (0.12 ±0.12), which could be significantly reduced by pretreatment with high, medium and low concentration ICA ((0.57±0.08), (0.63±0.02), (0.66±0.04) vs. (0.84±0.04), all P<0.05). (6) The expression of AKT/p-AKT was significantly lower in GSNO group than in the blank control group (P< 0.05), which could be significantly upregulated by pretreatment with high, medium and low concentration ICA in a concentration-dependent manner, above effects could be blocked by LY294002 (all P<0.05). (7) The expression of P53 was significantly higher in GSNO group than in the blank control group (P< 0.05), which could be significantly down regulated by pretreatment with high, medium and low concentration ICA in a concentration-dependent manner, above effects could be blocked by LY294002(all P<0.05). (8) The expression of CYC and caspase-3 was significantly reduced in the mitochondria and increased in cytoplasm post GSNO treatment compared to blank control group, which could be reversed by pretreatment with high, medium and low concentration ICA(all P<0.05). (9) The expression of eNOS/p-eNOS was similar between GSNO and the blank control group, while it was significantly upregulated by pretreatment with high, medium and low concentration ICA and this effect could be blocked by LY294002(all P<0.05). CONCLUSION: Icariin could reduce GSNO induced endothelial cell apoptosis through activating AKT pathway and downregulating P53 activity.

Majorana Positivity and the Fermion Sign Problem of Quantum Monte Carlo Simulations.

The sign problem is a major obstacle in quantum Monte Carlo simulations for many-body fermion systems. We examine this problem with a new perspective based on the Majorana reflection positivity and Majorana Kramers positivity. Two sufficient conditions are proven for the absence of the fermion sign problem. Our proof provides a unified description for all the interacting lattice fermion models previously known to be free of the sign problem based on the auxiliary field quantum Monte Carlo method. It also allows us to identify a number of new sign-problem-free interacting fermion models including, but not limited to, lattice fermion models with repulsive interactions but without particle-hole symmetry, and interacting topological insulators with spin-flip terms.

[Role and clinical significance of Th17/Treg balance in patients with severe exacerbation of hepatitis B].

OBJECTIVE: To investigate the role of Th17/Treg balance in immune mechanism in severe exacerbation of hepatitis B. METHODS: The clinical data of 41 patients with chronic hepatitis B were collected, and according to the conditions during hospitalization, these patients were divided into exacerbation group (19 patients) and improvement group (22 patients). On admission, at weeks 1 and 2 of treatment, and at the end of treatment, flow cytometry was used to measure the frequencies of Th17 and Treg cells in peripheral blood, and enzyme-linked immunosorbent assay was used to determine the serum levels of interleukin-17 (IL-17), interleukin-10 (IL-10), and transforming growth factor-ß (TGF-ß). The dynamic changes in the frequencies of Th17 and Treg cells were compared between the two groups, and the correlation between clinical indices for hepatitis and cytokines was analyzed. The t-test was used for comparison between groups, a one-way analysis of variance was used for comparison within one group across different time points, and Pearson correlation analysis was performed. RESULTS: With disease progression, the exacerbation group showed an increase in the frequency of Th17 cells and a relatively low frequency of Treg cells; compared with the improvement group, the exacerbation group had a higher frequency of Th17 cells and a lower frequency of Treg cells. Th17/Treg ratio gradually increased with exacerbation and decreased with improvement in conditions; in the exacerbation group and the improvement group, Th17/Treg ratio was positively correlated with total bilirubin and negatively correlated with prothrombin activity. In the exacerbation group and the improvement group, Th17 cells were positively correlated with IL-17, and Treg cells were positively correlated with IL-10 and TGF-ß. CONCLUSION: Th17 and Treg cells play important roles in severe exacerbation of hepatitis B, and Th17/Treg ratio may be used as an immunobiological marker for the judgment of severity during severe exacerbation of hepatitis B.

Application of single-step genomic evaluation for crossbred performance in pig.

Crossbreding is predominant and intensively used in commercial meat production systems, especially in poultry and swine. Genomic evaluation has been successfully applied for breeding within purebreds but also offers opportunities of selecting purebreds for crossbred performance by combining information from purebreds with information from crossbreds. However, it generally requires that all relevant animals are genotyped, which is costly and presently does not seem to be feasible in practice. Recently, a novel single-step BLUP method for genomic evaluation of both purebred and crossbred performance has been developed that can incorporate marker genotypes into a traditional animal model. This new method has not been validated in real data sets. In this study, we applied this single-step method to analyze data for the maternal trait of total number of piglets born in Danish Landrace, Yorkshire, and two-way crossbred pigs in different scenarios. The genetic correlation between purebred and crossbred performances was investigated first, and then the impact of (crossbred) genomic information on prediction reliability for crossbred performance was explored. The results confirm the existence of a moderate genetic correlation, and it was seen that the standard errors on the estimates were reduced when including genomic information. Models with marker information, especially crossbred genomic information, improved model-based reliabilities for crossbred performance of purebred boars and also improved the predictive ability for crossbred animals and, to some extent, reduced the bias of prediction. We conclude that the new single-step BLUP method is a good tool in the genetic evaluation for crossbred performance in purebred animals.

Interleukin-17 produced by tumor microenvironment promotes self-renewal of CD133+ cancer stem-like cells in ovarian cancer.

Inflammatory cytokines, components of cancer stem cells (CSCs) niche, could affect the characteristics of CSCs such as self-renewal and metastasis. Interleukin-17 (IL-17) is a new pro-inflammatory cytokine mainly produced by T-helper (Th17) cells and macrophages. The effects of IL-17 on the characteristics of CSCs remain to be explored. Here we first demonstrated a role of IL-17 in promoting the self-renewal of ovarian CD133(+) cancer stem-like cells (CSLCs). We detected IL-17-producing cells (CD4(+) cells and CD68(+) macrophages) in the niche of CD133(+)CSLCs. Meanwhile, there was IL-17 receptor expression on CD133(+)CSLCs derived from A2780 cell line and primary ovarian cancer tissues. By recombinant human IL-17 stimulation and IL-17 transfection, the growth and sphere formation capacities of ovarian CD133(+)CSLCs were significantly enhanced in a dose-dependent manner. Moreover, ovarian CD133(+)CSLCs transfected with IL-17 showed greater tumorigenesis capacity in nude mice. These data suggest that IL-17 promoted the self-renewal of ovarian CD133(+)CSLCs. Further investigation through gene profiling revealed that the stimulation function of IL-17 on self-renewal of ovarian CD133(+)CSLCs might be mediated by the nuclear factor (NF)-κB and p38 mitogen-activated protein kinases (MAPK) signaling pathway. NF-κB and p38 MAPK were activated by IL-17. More importantly, IL-17-promoted self-renewal was inhibited by specific inhibitors of NF-κB and p38 MAPK. Taken together, our data indicate that IL-17 contributed to ovarian cancer malignancy through promoting the self-renewal of CD133(+)CSLCs and that IL-17 and its signaling pathway might serve as therapeutic targets for the treatment of ovarian cancer.

Tensor renormalization group study of classical XY model on the square lattice.

Using the tensor renormalization group method based on the higher-order singular value decomposition, we have studied the thermodynamic properties of the continuous XY model on the square lattice. The temperature dependence of the free energy, the internal energy, and the specific heat agree with the Monte Carlo calculations. From the field dependence of the magnetic susceptibility, we find the Kosterlitz-Thouless transition temperature to be 0.8921(19), consistent with the Monte Carlo as well as the high temperature series expansion results. At the transition temperature, the critical exponent δ is estimated as 14.5, close to the analytic value by Kosterlitz.