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1.
Environ Sci Pollut Res Int ; 30(10): 26292-26307, 2023 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-36357759

RESUMEN

This study explores the relationship between Entrepreneurship Education (EE) and Entrepreneurial Intentions (EI) using the Theory of Planned Behavior (TPB). From January through May of 2022, students from 10 Chinese institutions were surveyed using an online questionnaire. According to the research, students' EI scores rose significantly after participating in EE. In addition, students in China had a more significant impact on EI regarding factors like perceived feasibility and desirability. This study extends the body of knowledge about the connection between prior exposure and early intervention (EI) by demonstrating the beneficial effects of PE on EI. In addition, the results suggest that girls have lower EI than males, which is good news for gender equality. Lastly, the behavioral entrepreneur attitude has a favorable correlation with EI. Policymakers and university administrators might use the findings to understand better how and when extracurricular activities (EE) improve students' emotional intelligence (EI). A pioneering empirical study in a developing South-Asian setting shows the relevance of EE on EI among students at private universities. According to the study, EE generates EI, and entrepreneurial enthusiasm is crucial.


Asunto(s)
Emprendimiento , Intención , Estudiantes , Femenino , Humanos , Masculino , Pueblo Asiatico , China , Escolaridad , Teoría del Comportamiento Planificado
2.
Front Psychol ; 13: 800936, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-35795425

RESUMEN

Green Human Resource Management (HRM) supports promoting and incorporating sustainable development with regard to their resources. Managers and customers actively utilize the limited resources efficiently and effectively to accomplish environment-friendly goals and objectives. The study focuses on investigating the moderating role of diffidence between Green HRM, among eco-friendly behavior and Employee Performance of frontline employees of the hospitality sector. Two hundred ten individuals particapted in the research from hospitality sector with regard to examine green HRM policies of employees along with their influence on consumer buying behvaiour. Frontline employees incorporated the data on HRM performance, eco-friendly behavior, and diffidence. Besides, consumers gave their opinion on consumer buying behavior. The study's findings revealed that Green HRM, aka Green HRM, directly impacts consumer behavior. In addition to this diffidence moderates the relationship between Green HRM and employee performance and employee eco-friendly behavior. Besides, future studies can explore the clothing and banking sector as the current study was conducted in fast food sector.

3.
Acta Histochem ; 122(1): 151466, 2020 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-31787253

RESUMEN

BACKGROUND: Anorectal malformations (ARMs) are common congenital malformations of the terminal digestive tract, but little is known regarding their pathogenesis. Aberrant cell proliferation/apoptosis are believed to be involved in ARMs. However, there are no studies on proliferation/apoptosis-related genes. PURPOSE: We aimed to investigate the spatiotemporal expression patterns of two proliferation/apoptosis-related genes (MYC proto-oncogene and tumor protein p53) and explore their potential functions in the hindguts of ethylene thiourea-induced ARMs rat fetuses. METHODS: MYC and p53 expression was evaluated using immunohistochemical staining, western blotting, and quantitative real-time polymerase chain reaction (RT-qPCR). Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) and p53 costaining were performed to assay the colocalization of apoptotic and p53-expressing cells. RESULTS: Rat fetuses with ARMs displayed fusion failure of the urogenital septum and cloacal membrane. In the control group, MYC was persistently expressed from gestational day (GD)14 to GD16 and distributed throughout the hindgut, while p53 was weakly detected in the terminal segment of the urethra and hindgut; in the ARMs group, MYC expression was obviously reduced, while p53 was widely and highly expressed in the urethra and hindgut. Western blotting and RT-qPCR confirmed the decrease in MYC and increase in p53 expression in ARMs. TUNEL and p53 co-staining revealed considerable overlap between apoptotic and p53-expressing cells. CONCLUSION: The expression patterns of c-myc and p53 were disrupted in ARMs rat embryos, and the downregulation of c-myc and upregulation of p53 might be related to the development of ARMs at the key time points of ARMs morphogenesis.


Asunto(s)
Malformaciones Anorrectales , Etilenotiourea/toxicidad , Feto , Regulación del Desarrollo de la Expresión Génica/efectos de los fármacos , Intestinos , Proteínas Proto-Oncogénicas c-myc/biosíntesis , Proteína p53 Supresora de Tumor/biosíntesis , Animales , Malformaciones Anorrectales/inducido químicamente , Malformaciones Anorrectales/embriología , Malformaciones Anorrectales/patología , Apoptosis/efectos de los fármacos , Proliferación Celular/efectos de los fármacos , Femenino , Feto/embriología , Feto/patología , Intestinos/embriología , Intestinos/patología , Embarazo , Ratas , Ratas Wistar
4.
PeerJ ; 7: e6829, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31065464

RESUMEN

BACKGROUND: Quantitative real-time polymerase chain reaction (RT-qPCR) is a sensitive method for quantifying mRNA abundance. With relative expression analysis, however, reliable data output is dependent on stably expressed reference genes across the samples being studied. In anorectal malformations (ARMs), there is limited data on the selection of appropriate reference genes. PURPOSE: This study was aimed to investigate the optimal reference genes for PCR in ARM rat models. METHODS: We selected 15 commonly used reference genes (Rps18, Actb, B2m, Gapdh, Ppia, Hprt1, Pgk1, Ywhaz, Tbp, Ubc, Rps16, Rpl13a, Rplp1, Sdha, and Hmbs) as candidate reference genes and detected their mRNA expression in ARM samples by RT-qPCR. The expression stability and variability of these transcripts were subsequently evaluated using four methods (geNorm, NormFinder, comparative ΔCt, and BestKeeper). RESULTS: The abundance of the candidate reference genes was qualified by RT-qPCR and the cycle threshold (Ct) values ranged between 14.07 (Rplp1) and 21.89 (Sdha). In the overall candidate genes, different variations existed across the different algorithms. A comprehensive analysis revealed that Rpl13a ranked first among the relatively stable genes, followed by Ywhaz, Rps18, Sdha, and Hmbs. CONCLUSIONS: The most stable reference genes for RT-qPCR were Rpl13a, Ywhaz, and Rps18 in ETU-induced ARMs in rat fetus. This study provided a foundation for reference gene selection for future gene expression analyses.

5.
Langmuir ; 25(6): 3646-51, 2009 Apr 09.
Artículo en Inglés | MEDLINE | ID: mdl-19708147

RESUMEN

A novel azobenzene-derived amphiphile with a melamine head, 2Azo-2C12H2-melamine, has been synthesized. pi-A isotherm measurements displayed that this amphiphile is able to form a stable Langmuir monolayer on both pure water and barbituric acid (BA)- or thymine (T)-containing subphases. The collapse surface pressure and limiting molecular area of its Langmuir monolayer on pure water are 40 mN/m and 0.56 nm2, respectively. However, when barbituric acid or thymine was introduced into the subphase, the corresponding pi-A isotherms of the monolayers exhibited a lower collapse surface pressure (22 mN/m for BA, 21 mN/m for T) and smaller limiting molecular area (0.54 nm2 for BA and 0.52 nm2 for T). UV-vis and FT-IR studies of the LB films formed by 2Azo-2C12H25-melamine have also been carried out. The results indicated that the LB films of 2Azo-2C12H25-melamine deposited from pure water undergo distinct collapse of the H-aggregate upon UV irradiation, while the LB films deposited from a BA- or T-containing subphase retain the H-aggregate. The host-guest-interaction-induced blockage of azobenzene photoisomerization should be responsible for the stabilized H-aggregate. A 1:1 host/guest binding mode to form a linear supramolecular polymeric chain has been proposed in the Langmuir monolayers formed on a BA- or T-containing subphase. The current results suggest that the host-guest interaction should be an effective means to manipulate the interfacial assembly of azobenzene-derived amphiphiles.

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