Influence of psychological intervention on patients undergoing spinal anesthesia: a randomized trial.

OBJECTIVE: The aim of this study is to evaluate the effects of positive intervention on the anxiety and the physiological and psychological aspects among preoperative and post-surgical patients with spinal anesthesia. PATIENTS AND METHODS: A randomized trial was conducted with an intervention group (n=58) and a control group (n=59). In the intervention group, the patients were well-informed of the details during spinal anesthesia. Multiple methods were performed to control anxiety before surgery, and nurses were not allowed to discuss the condition during surgery. Anesthesiologists were invited to visit patients to avoid excessive anxiety. RESULTS: The intervention group showed lower scores of State-Trait Anxiety Inventory (STAI) (p<0.05) than the control group 24 hours post-operation. Physiological indices such as systolic blood pressure, low frequency (LF) power, high frequency (HF) power and ration of LF/HF showed better surgery recovery (p<0.05) than the control group. The length of post-anesthesia care unit stay was also significantly shortened in the intervention group (p=0.001) compared with the control group. Positive intervention may alleviate the anxiety in surgical patients receiving spinal anesthesia and improve the physiological and psychological outcomes clinically. CONCLUSIONS: Our results provide evidence indicating that proper intervention can be promoted clinically to improve the satisfaction and quality of life of patients undergoing spinal anesthesia.

Acoustic manipulation dynamics of levitated particle with screw-shaped reflecting surface.

Existing single-axis acoustic levitation devices with an axisymmetric reflector can manipulate particles in a variety of ways. However, the mechanism by which particles are suspended in a single-axis acoustic levitator with a non-axisymmetric reflector remains poorly understood. This work addresses this issue by proposing a novel single-axis ultrasonic levitator design that includes a flat plane emitter and a screw-plane reflector. The node positions of the standing wave formed in this levitator were predicted by calculating the Gor'kov potential according to a numerical model. The analysis results demonstrate that the nodes were distributed off-axis and their positions varied in a spiral manner when changing the distance between the emitter and reflector. Corresponding experiments based on the proposed design were also conducted, and the results indicated that the distance changes between the emitter and reflector could induce some spiral trajectories of a polyethylene-foam particle placed in the ultrasonic field. Moreover, the trajectory of the suspended particle was found to distribute along a conical surface centered on the central axis of this device. This work provides a new approach for ultrasonic particle manipulation by changing the geometry of the reflector.

Incidence and familial clustering of infantile haemangiomas: a multicentre study.

BACKGROUND: The exact incidence of infantile haemangiomas (IH) in the Chinese population is still unknown. A positive family history of IH was considered as a risk factor for the development of IH. OBJECTIVES: This study aims to investigate the incidence of IH in the Chinese population and the mechanism of family history increases the risk for IH development. METHODS: A total of 2489 women and their newborns were enrolled in the prospective study. All newborns were followed up for 12 months to determine whether they developed IH. In addition, 213 IH probands and their 174 siblings were enrolled in the study. The incidence of IH in siblings of the IH probands was investigated. Information regarding risk factors for IH and demographic data were collected on all children. RESULTS: Of the 2572 newborns, 58 IH were identified in 56 (2.2%) newborns. The majority of IH were located on the trunk (46.6%). Siblings of the IH probands were at increased risk for the development of IH (P = 0.024, relative risk 2.451), and the occurrence of prenatal risk factors for IH(P = 0.003) compared with the general population. CONCLUSIONS: Our study showed that the incidence of IH is 2.2% in the Chinese population. Siblings of the individuals with IH were at increased risk for the development of IH may be related to the family clustering of prenatal risk factors for IH. Further exploration of the mechanisms and common features of these prenatal risk factors may help to disclose the origin and pathogenesis of IH.

Transcriptome characterization of HPG axis from Chinese sea perch Lateolabrax maculatus.

Here the transcriptome and differential gene expression in the adult brain and gonads of the Chinese sea perch Lateolabrax maculatus were reported. A total of 78 256 909 clean reads were generated from the adult brain, ovary and testis by using the Illumina HiSeq2000 platform and assembled into 274 909 contigs. A total of 31 683 unigenes were annotated based on sequence similarity and 20 702 unigenes were found to exhibit 8237 gene ontology terms and 3888 signal pathways. Transcripts of 26 623 unigenes were present in all of the tissues, whereas pairwise comparisons revealed that 671/367, 496/315 and 1668/580 unigenes were up-down regulated by at least two-fold between the brain and ovary, ovary and testis and brain and testis, respectively. Homology search led to the identification of reproduction-associated genes of the brain-gonad axis, including those involved in sex differentiation and maintenance. The data provided an integrated and comprehensive transcriptome resource for L. maculatus, which could be used for further research on hypothalamus-pituitary-gonad axis gene function, reproduction regulation and sex-biased gene expression.

A numerical simulation of acoustic field within liquids subject to three orthogonal ultrasounds.

When one beam of ultrasound propagates along a single direction in liquids, the cavitation effect is always confined to a limited volume close to the ultrasonic source. This greatly limits the application of power ultrasound in liquid processing and materials fabrication. In this study, a methodology for applying three orthogonal ultrasounds within liquids has been proposed. By solving the Helmholtz equation, the sound field distribution characteristics are investigated in 1D (one dimensional), 2D (two dimensional) and 3D (three dimensional) ultrasounds at their resonant frequencies, which show that the coherent interaction of three beams of ultrasounds is able to strikingly promote the sound pressure level and reinforce the mean acoustic energy density as compared with that in 1D case. Hence, the potential cavitation volume is enlarged remarkably. This opens new possibilities for the design and optimization of ultrasonic technology in fabricating materials.

Acoustic levitation with self-adaptive flexible reflectors.

Two kinds of flexible reflectors are proposed and examined in this paper to improve the stability of single-axis acoustic levitator, especially in the case of levitating high-density and high-temperature samples. One kind is those with a deformable reflecting surface, and the other kind is those with an elastic support, both of which are self-adaptive to the change of acoustic radiation pressure. High-density materials such as iridium (density 22.6 gcm(-3)) are stably levitated at room temperature with a soft reflector made of colloid as well as a rigid reflector supported by a spring. In addition, the containerless melting and solidification of binary In-Bi eutectic alloy (melting point 345.8 K) and ternary Ag-Cu-Ge eutectic alloy (melting point 812 K) are successfully achieved by applying the elastically supported reflector with the assistance of a laser beam.

Reproductive biology of two Himalayan alpine gingers (Roscoea spp., Zingiberaceae) in China: pollination syndrome and compensatory floral mechanisms.

According to the concept of pollination syndromes, floral traits reflect specialisation to a particular pollinator or set of pollinators. However, the reproductive biology of endemic, and often specialised, plants may require increased attention as climate change accelerates worldwide. Species of Roscoea endemic to the Himalayan region have striking orchid-like flowers with long corolla tubes, suggesting pollination by long-tongued insects. Until now, the reproductive biology of species of Roscoea has been poorly documented. We investigated the floral biology, breeding system and pollination ecology of R. cautleoides and R. humeana, from Hengduan Mountains, a global biodiversity hotspot in southwest China. We also tested whether floral longevity increases pollination success. Pollination experiments showed that the two species were self-compatible and depended on insects for fruit production. Over several flowering seasons we did not observe any potential pollinators with long tongues that matched the corolla tube visiting flowers in centres of distribution. The principal pollinators observed were pollen-collecting generalist bees, with low visitation frequencies. In general, members of the ginger family are characterised by short-lived (usually 1 day) flowers, but flowers of R. cautleoides and R. humeana last 8 and 6 days, respectively. Removing stigmas decreased fruit set in both study populations. Our results suggest that the original pollinators may have been long-tongued insects that are now absent from the Chinese Himalayas because habitats have responded to climate change. However, long-lived and self-compatible flowers, coupled with the presence of generalist pollinators, are traits that have allowed these gingers to reproduce and continue to persist in the alpine habitats.

Parametrically excited sectorial oscillation of liquid drops floating in ultrasound.

We report experiments in which the nonaxisymmetric sectorial oscillations of water drops have been excited using acoustic levitation and an active modulation method. The observed stable sectorial oscillations are up to the seventh mode. These oscillations are excited by parametric resonance. The oblate initial shape of the water drops is essential to this kind of excitations. The oscillation frequency increases with mode number but decreases with equatorial radius for each mode number. The data can be well described by a modified Rayleigh equation, without the use of additional parameters.

Examination of the MSX1 gene in patients with Parkinson's disease.

BACKGROUND: Several genetic variants in transcription factor genes have been reported to be associated with Parkinson's disease (PD). The muscle segment homeobox drosophila homolog of 1 gene (MSX1) is a major upstream regulator of the dopaminergic neuronal subtype specification. AIMS OF THE STUDY: To determine whether genetic variation in the coding region of the MSX1 gene plays a role in the etiology of PD. METHODS: We searched for genetic variations in the coding region of the MSX1 gene in 202 patients with PD and 200 normal controls by PCR-single-strand conformation polymorphism (PCR-SSCP) and sequencing. RESULTS: No mutation in the MSX1 gene was identified in our cohort. CONCLUSIONS: Mutations in the coding region of the MSX1 gene play little or no role in the development of PD.

Polyglycolic Acid-islet grafts improve blood glucose and insulin concentrations in rats with induced diabetes.

Pancreatic islet transplantation is a promising therapeutic treatment for type 1 diabetes mellitus. In the present study, we cocultured islets with or without a polyglycolic acid (PGA) fibrous scaffold for 5 days and transplanted the PGA-islet grafts into the leg muscles of Wistar rats with streptozotocin-induced diabetes; controls were injected with saline. The results showed that the blood glucose concentrations of the group given islets embedded with the PGA scaffold were lower than those without the scaffold or controls. On the other hand, the insulin content of the PGA-islet group was higher at all 5 time points compared with the insulin contents of the other 2 groups. After transplantation, many islets in the PGA-islet grafts showed normal morphology (as seen under the scanning electron microscope) and were surrounded by red blood cells. A fibrous extracellular matrix was visible around the PGA-islet grafts. These results demonstrated that PGA-islet grafts improved blood glucose and insulin concentrations in rats with induced diabetes.

SO2 removal with ferric sulfate solution.

The determination of the influence of the concentration of ferric sulfate solution on SO2 absorption was performed in this study. It was found that the SO2 absorption efficiency increased with increasing ferric concentration, and decreased with the acidity of the spraying solution. As the hydrolysis of ferric ions occurs in solution, the SO2 removal efficiency increased slowly with increasing Fe(III) concentration. Taking into account the hydrodynamic and mass transfer characteristics of the packed column, the enhancement factor (E) was found to depend on the concentration of the ferric ions and pH, which indicated that it could be used for the simulation or design of SO2 scrubbers.

Adhesive growth of pancreatic islet cells on a polyglycolic acid fibrous scaffold.

BACKGROUND: The use of cultured pancreatic islet cells for diabetes treatment offers several advantages. In theory, cultured cells show greater purity and lower immunogenicity. However, cultured islet cells display a low survival rate in vitro. In the present study we grew islet cells on a polyglycolic acid (PGA) fibrous scaffold to promote cell adhesion, growth, and viability during prolonged culture. METHODS: Islets isolated from Wistar rat pancreata were digested with collagenase and purified by the Ficoll method. Cells were grown in culture with or without PGA scaffolds. Islet cell purity was determined using a dithizone stain; viability and survival rates were determined using an AO-PI stain. The insulin-secretion index was detected using radioimmunodetection and the growth on an adhesive scaffold analyzed using an inverted microscope and scanning electron microscope (SEM). RESULTS: In contrast to the scaffold-free control group, cells cultured on PGA scaffolds exhibited improved morphology, less cell death, and prolonged survival times. Cell viability and survival rates were significantly increased in scaffolded cells when compared to control cells (P < .05). Increased insulin secretion was observed in the culture solution of scaffolded cells following stimulation with low glucose (5.6 mmol/L) versus high glucose (16.7 mmol/L). The secretion indices of the two groups were significantly different (P < .05). Islet cell growth, as observed under SEM, was tightly circumvolute, adhesive, and three-dimensional. CONCLUSIONS: The present results demonstrated that islet cells can successfully grow and survive in culture on a PGA scaffold. These cells exhibited enhanced viability, survival, and insulin secretion.

Examination of the SLITRK1 gene in Caucasian patients with Tourette syndrome.

OBJECTIVE: To determine whether variants in the Slit and Trk-like 1 gene (SLITRK1) are present in American Caucasian population of patients with Tourette syndrome (TS). METHODS: We sequenced the 3'-untranslated region for var321 and the whole coding region in the SLITRK1 gene in 82 Caucasian patients with TS from North America. RESULTS: None of the 82 samples from patients with TS showed the non-coding sequence variant (var321). Only one patient with familial TS was heterozygous for a novel 708C > T (Ile236Ile) nucleotide variant. CONCLUSIONS: The var321 and mutation(s) in the coding region of the SLITRK1 gene probably are a rare cause of TS in a Caucasian population; therefore, genetic heterogeneity of TS should be considered. Tests designed to detect variant(s) in the SLITRK1 gene probably will not have a diagnostic utility in clinical practice.

Projections of diencephalic dopamine neurons into the spinal cord in mice.

The aim of this study is to investigate the pathway of diencephalic dopaminergic (DA) neuronal innervating into the spinal cord in mice, the pathway is postulated relevant to clinical restless legs syndrome (RLS). Tyrosine hydroxylase (TH) immunohistochemistry was used to identify the DA neuron. The fluorescent tracer Fluoro-Gold (FG) was stereotaxically injected into the T10-L5 spinal cord of CBL57 mice (n=20) seven days before the animals were sacrificed. The diencephalic sections were stained with TH antibody and the FG tracer present in the diencephalic DA neurons were examined under fluoresce microscope. The average number of total DA neurons per side in A11, A12, A13 and A14 was 66+/-8, 221+/-12, 350+/-17 and 254+/-21 respectively. After being injected into the spinal cord, FG reached the DA neurons within the A10 and A11 groups, but didn't target to any other DA neuron groups including the A8 and A9 groups in substantia nigra (SN). The diencephalic A11 DA neurons possessed long axons extending over several segments and possibly traversing the entire length of the spinal cord. It is the first time to report A10 and A11 DA neuron projections into the spinal cord in mice.

Dynamics of acoustically levitated disk samples.

The acoustic levitation force on disk samples and the dynamics of large water drops in a planar standing wave are studied by solving the acoustic scattering problem through incorporating the boundary element method. The dependence of levitation force amplitude on the equivalent radius R of disks deviates seriously from the R3 law predicted by King's theory, and a larger force can be obtained for thin disks. When the disk aspect ratio gamma is larger than a critical value gamma(*) ( approximately 1.9 ) and the disk radius a is smaller than the critical value a(*) (gamma) , the levitation force per unit volume of the sample will increase with the enlargement of the disk. The acoustic levitation force on thin-disk samples ( gamma

Dependence of acoustic levitation capabilities on geometric parameters.

A two-cylinder model incorporating boundary element method simulations is developed, which builds up the relationship between the levitation capabilities and the geometric parameters of a single-axis acoustic levitator with reference to wavelength. This model proves to be successful in predicting resonant modes of the acoustic field and explaining axial symmetry deviation of the levitated samples near the reflector and emitter. Concave reflecting surfaces of a spherical cap, a paraboloid, and a hyperboloid of revolution are investigated systematically with regard to the dependence of the levitation force on the section radius R(b) and curvature radius R (or depth D) of the reflector. It is found that the levitation force can be remarkably enhanced by choosing an optimum value of R or D, and the possible degree of this enhancement for spherically curved reflectors is the largest. The degree of levitation force enhancement by this means can also be facilitated by enlarging R(b) and employing a lower resonant mode. The deviation of the sample near the reflector is found likely to occur in case of smaller R(b), larger D, and a higher resonant mode. The calculated dependence of levitation force on R, R(b), and the resonant mode is also verified by experiment and finally demonstrated to be in good agreement with experimental results, in which considerably a strong levitation force is achieved to levitate an iridium sphere which has the largest density of 22.6 g/cm(3).

Levitation of iridium and liquid mercury by ultrasound.

Single-axis acoustic levitation of the heaviest solid (iridium, rho=22.6 g cm(-3)) and liquid (mercury, rho=13.6 g cm(-3) on the Earth is achieved by greatly enhancing both the levitation force and stability through optimizing the geometric parameters of the levitator. The acoustically levitated Pb-Sn eutectic alloy melt (rho=8.5 g cm(-3)) is highly undercooled by up to 38 K, which results in a microstructural transition of "lamellae-broken lamellae-dendrites." The drastic enhancement of levitation capability indicates a broader application range of single-axis acoustic levitation.