Impacts of Perioperative Comprehensive Nursing Intervention on Postoperative Urinary Incontinence and Quality of Life of Patients Undergoing Laparoscopic Radical Prostatectomy.

To evaluate the impact of perioperative comprehensive nursing intervention on postoperative urinary incontinence, various aspects of patient well-being were assessed. The comprehensive group, that received the nursing intervention, demonstrated significant improvements in self-care skills, health knowledge level, self-care responsibility, and self-concept compared to the standard group. The findings indicate that perioperative comprehensive nursing intervention has a remarkable effect on patients undergoing laparoscopic radical prostatectomy. This nursing intervention not only effectively improves postoperative urinary incontinence and alleviates negative emotions, such as anxiety and depression. Therefore, the implementation of this nursing intervention model is highly recommended for clinical practice and wider application.

Novel mechanism of hydrogen peroxide for promoting efficient natamycin synthesis in Streptomyces.

The mechanism of regulation of natamycin biosynthesis by Streptomyces in response to oxidative stress is unclear. Here, we first show cholesterol oxidase SgnE, which catalyzes the formation of H2O2 from sterols, triggered a series of redox-dependent interactions to stimulate natamycin production in S. gilvosporeus. In response to reactive oxygen species, residues Cys212 and Cys221 of the H2O2-sensing consensus sequence of OxyR were oxidized, resulting in conformational changes in the protein: OxyR extended its DNA-binding domain to interact with four motifs of promoter p sgnM . This acted as a redox-dependent switch to turn on/off gene transcription of sgnM, which encodes a cluster-situated regulator, by controlling the affinity between OxyR and p sgnM , thus regulating the expression of 12 genes in the natamycin biosynthesis gene cluster. OxyR cooperates with SgnR, another cluster-situated regulator and an upstream regulatory factor of SgnM, synergistically modulated natamycin biosynthesis by masking/unmasking the -35 region of p sgnM depending on the redox state of OxyR in response to the intracellular H2O2 concentration. IMPORTANCE Cholesterol oxidase SgnE is an indispensable factor, with an unclear mechanism, for natamycin biosynthesis in Streptomyces. Oxidative stress has been attributed to the natamycin biosynthesis. Here, we show that SgnE catalyzes the formation of H2O2 from sterols and triggers a series of redox-dependent interactions to stimulate natamycin production in S. gilvosporeus. OxyR, which cooperates with SgnR, acted as a redox-dependent switch to turn on/off gene transcription of sgnM, which encodes a cluster-situated regulator, by masking/unmasking its -35 region, to control the natamycin biosynthesis gene cluster. This work provides a novel perspective on the crosstalk between intracellular ROS homeostasis and natamycin biosynthesis. Application of these findings will improve antibiotic yields via control of the intracellular redox pressure in Streptomyces.

MacRS controls morphological differentiation and natamycin biosynthesis in Streptomyces gilvosporeus F607.

Streptomyces gilvosporeus F607 produces large amounts of natamycin in a process regulated by multiple networks, including two-component systems (TCSs). The macR and macS genes, which are annotated as rs12540 and rs12545, respectively, in S. gilvosporeus F607, affect natamycin biosynthesis and sporulation. The findings of this study indicate that deletion of macRS from S. gilvosporeus F607 prevents the production of natamycin, delays spore formation (according to scanning electron microscopy), and results in aerial hyphae lacking compartments separated by septa (according to transmission electron microscopy). Real-time quantitative polymerase chain reaction (RT-qPCR) analyses revealed that the expression levels of natamycin biosynthesis-related genes and genes essential for septum formation during sporulation were affected in the ΔmacRS mutant strain. Molecular simulations and electrophoretic mobility shift assays (EMSAs) suggested MacR not only interacted with the intergenic region of sgnM and sgnR, but also with the promoter of penicillin-binding protein gene ftsL required for cell division. sgnR promoter was presumed to be the binding target of MacR based on the RT-qPCR results. MacR had different affinity with two binding sites: one was located at ftsL promoter region with a perfect inverted repeats 'TGAGTACGCGTACTCA', the other was located at the presumed sgnR promoter with an imperfect inverted repeats 'TGAAGGTGCTGGACTCA'. We propose a hypothesis of a three-level regulatory pathway based on pleiotropic transcriptional regulator MacR and its target genes sgnR and ftsL; the pathway activates natamycin biosynthesis and influences septum development via direct and indirect effects in S. gilvosporeus F607.