RESUMEN
A convenient, fast and non-invasive portable electrochemical uricometer (PUM) assisted with the uricase-packaged nanoflowers (NFs) was constructed for continually and accurately monitoring of uric acid (UA) in urine samples at random intervals in just 20 s. Only a small amount of urine (50 µL) was needed for each test. Electrochemical deposition was adopted to modify gold nanoparticles (AuNPs) on screen-printed carbon electrodes (SPCE) and uricase-inorganic hybrid NFs (UOx-NFs) induced by calcium ions (Ca2+) were introduced for UA detection with expected specificity. Cyclic voltammetry (CV) (detection limit of 8.87 µM and liner range of 0-4 mM) and amperometry (detection limit of 0.82 µM and liner range of 0-5 mM) protocols were studied for UA detection, respectively. Finally, the uric acid in urine had be successfully continually monitored from volunteers with various dietary choosing, the results of which can be adopted as the effective evidence for uric acid control.
Asunto(s)
Técnicas Biosensibles , Nanopartículas del Metal , Técnicas Electroquímicas , Electrodos , Oro , Humanos , Urato Oxidasa , Ácido ÚricoRESUMEN
Development of personal glucose meters (PGMs) for blood glucose monitoring and management by the diabetic patients has been a long history since its first invention in 1968 and commercial application in 1975. The main reasons for its wide acceptance and popularity can be attributed mainly to the easy operation, test-to-result model, low cost, and small volume of sample required for blood glucose concentration test. During past decades, advances in analytical techniques have repurposed the use of PGMs into a general point-of-care testing platform for a variety of non-glucose targets, especially the food hazards. In this review, we summarized the recent published research using PGMs to detect the food safety hazards of mycotoxins, illegal additives, pathogen bacteria, and pesticide and veterinary drug residues detection with PGMs. The progress on PGM-based detection achieved in food safety have been carefully compared and analyzed. Furthermore, the current bottlenecks and challenges for practical applications of PGM for hazards detection in food safety have also been proposed.
Asunto(s)
Micotoxinas , Plaguicidas , Drogas Veterinarias , Glucemia , Automonitorización de la Glucosa Sanguínea/métodos , Inocuidad de los Alimentos , Glucosa , Humanos , Plaguicidas/análisisRESUMEN
Herein, we proposed a terminal deoxynucleotidyl transferase (TdT), a potential biomarker of lymphoid tumors, responsive fluorescence polarization (FP)- sensing protocol based on framework nucleic acid (FNA)-wrapped protein-inorganic hybrid nanoflowers. To achieve this goal, a pair of poly-A-composed extension primers (EPa and EPb) was designed, and protein-inorganic hybrid nanoflowers were synthesized by a biomineralization reaction. EPa was labeled with carboxyfluorescein (FAM) fluorophore to create the preliminary FP signal. EPb was labeled with biotin to conjugate with hybrid nanoflowers. Upon introduction of TdT into the dTTP pool, both EPa and EPb can be catalyzed by TdT to incorporate numerous T bases, thereby facilitating intermolecular hybridization between 'A' and 'T' bases. The final assembled FNA-wrapped hybrid nanoflowers with greatly enhanced molecular volume and weight restrict the free rotation of attached FAMs, causing a great FP enhancement from a designated three-stage FP amplification. Under optimized conditions, the TdT can be detected with a detection limit of 0.023 U/mL and a linear detection from 0.1 U/mL to 100 U/mL within 20 min. As a proof-of-concept study, the first exploitation of FNA and protein-inorganic nanoflowers to improve the FP signal and the merit of FP without sample separation and washing opens a new avenue for biochemical study and disease diagnosis.