RESUMEN
Objective To establish an HPLC method for the simultaneous content determination of quercetin and kaempferol in Penthorum Chinense Pursh. Methods The HPLC analysis was carried out on Kromasil C18 (250 mm× 4.0 mm, 5.0 μm) with a mixture of methanol-0.1% phosphate (50:50) as the mobile phase. The determination wavelength was set at 360 nm with the flow rate of 1.0 mL/min. The column temperature was set at room temperature. Results The quercetin and kaempferol showed good linearity in the range of 0.105 6–2.112 0 μg and 0.023 6–0.472 μg respectively. The average recovery rates of quercetin and kaempferol were 98.46% (RSD=2.29%) and 98.17%(RSD=1.99%) respectively. Conclusion The method is accurate and sensitive, which can be used for the content determination of quercetin and kaempferol in Penthorum Chinense Pursh.
RESUMEN
A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L(-1) sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes.
Asunto(s)
Venenos de Anfibios/química , Bufanólidos/análisis , Electrocromatografía Capilar/métodos , Medicina Tradicional China , Preparaciones Farmacéuticas/química , Animales , Anuros , Electrocromatografía Capilar/instrumentación , Emulsiones/química , Concentración de Iones de Hidrógeno , Conformación Molecular , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Dodecil Sulfato de Sodio/química , Estereoisomerismo , Temperatura , Factores de TiempoRESUMEN
The present paper describes the development of a microemulsion electrokinetic chromatographic (MEEKC) method for simultaneous determination of andrographolide and dehydroandrographolide in traditional Chinese medicines and Chinese medicinal preparations. The MEEKC method involved the use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent and butan-1-ol as co-solvent. The effect of temperature and pH of running buffers on separation were examined. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w) and 10mM sodium tetraborate buffer, pH 9.2) allowed a useful and good reproducible separation of the studied analytes to be achieved.
Asunto(s)
Andrographis/metabolismo , Química Farmacéutica/métodos , Cromatografía Capilar Electrocinética Micelar/métodos , Diterpenos/análisis , Medicamentos Herbarios Chinos/análisis , Antiinflamatorios no Esteroideos/análisis , Butanoles/química , Calibración , Técnicas de Química Analítica/métodos , Emulsiones , Concentración de Iones de Hidrógeno , Modelos Químicos , Preparaciones Farmacéuticas/análisis , Dodecil Sulfato de Sodio/química , Solventes , Temperatura , Factores de TiempoRESUMEN
Broad spectrum anthelmintic agent-albendazole sulfoxide (ABZSO) have been separated and semiprepared on amylose tris (3,5-dimethylphenylcarbamate) chiral stationary phases by HPLC using mobile phases contained with n-hexane and different alcohols. For analytical separation the influence of the nature and content of alcoholic modifiers on separation were systemically studied. Then, the analytical methods were scaled up to semipreparative loading to obtain small quantities (about 1 g) of both ABZSO enantiomers. Especially, different loading amounts were investigated for their effect on various parameters of semipreparative HPLC. In addition, optical rotation and circular dichroism (CD) of both ABZSO enantiomers collected were determined and single enantiomers were found stable in configuration for 1 year.
Asunto(s)
Albendazol/análogos & derivados , Albendazol/química , Albendazol/aislamiento & purificación , Amilosa/análogos & derivados , Amilosa/química , Cromatografía Líquida de Alta Presión/métodos , Fenilcarbamatos/química , Isomerismo , Transición de FaseRESUMEN
The procedure for Rhizoma Pinelliae(Processed with Licorice and Lime) was combinatively evaluated by L_9(3)~4 orthogonal design with three factors:decocting times for Radix Glycyrrhizae,concentrated volume of decoction and macerating time. The optimum condition is as follows: each 100g of Rhizoma Pinelliae,accompanied with 15g of Radix Glycyrrhizae (decocted with water for two times,then the two decoctions were combined,and concentrated to 150ml.)and 10g of calx,was macerated in concentrated decoction of Radix Glyeyrrhizae for six days
RESUMEN
Objective: To determine the contents of cinobufagin and resibufogenin in Qianglijiuxin Dripping Pills. Methods: The HPLC method was used at Kromasil C 18 column of 250mm?4.6mm, 5?m, 0.5% Dipotassium Hydrogen Phosphate Acetonitrile (50∶50) as the mobile phase, 1.0mL/min as the flow rate at detection wavelength of 296nm. Results: For cinobufagin, the linear range was 0.67?g～4.7?g and the average recovery rate was 97.78% with RSD=1.2%. For resibufogenin, the linear range was 0.33?g～2.3?g and the average recovery was rate 99.61% with RSD=2.0%.Conclusion: The method can be applied to content determination of cinobafagin and Resibufogenin in Qianglijiuxin Dripping pills.
