Dissolved organic carbon spurs bacterial-algal competition and phosphorus-paucity adaptation: Boosting Microcystis' phosphorus uptake capacity.

Dissolved organic carbon (DOC) can alter the availability of background nutrients by affecting the proliferation of heterotrophic bacteria, which exerts a notable influence on algal growth and metabolism. However, the mechanism of how allochthonous DOC (aDOC) precipitates shifts in bacterial-algal interactions and modulates the occurrence of cyanobacteria blooms remains inadequately elucidated. Therefore, this study investigated the relationship between bacteria and algae under aDOC stimulation. We found that excess aDOC triggered the breakdown and reestablishment of the equilibrium between Microcystis and heterotrophic bacteria. The rapid proliferation of heterotrophic bacteria led to a dramatic decrease in soluble phosphorus and thereby resulted in the inhibition of the Microcystis growth. When the available DOC was depleted, the rapid death of heterotrophic bacteria released large amounts of dissolved phosphorus, which provided sufficient nutrients for the recovery of Microcystis. Notably, Microcystis rejuvenated and showed higher cell density compared to the carbon-absent group. This phenomenon can be ascribed that Microcystis regulated the compositions of extracellular polymeric substances (EPS) and the expression of relevant proteins to adapt to a nutrient-limited environment. Using time of flight secondary ion mass spectrometry (TOF-SIM) and proteomic analysis, we observed an enhancement of the signal of organic matter and metal ions associated with P complexation in EPS. Moreover, Microcystis upregulated proteins related to organic phosphorus transformation to increase the availability of phosphorus in various forms. In summary, this study emphasized the role of DOC in algal blooms, revealing the underestimated enhancement of Microcystis nutrient utilization through DOC-induced heterotrophic competition and providing valuable insights into eutrophication management and control.

Advanced design and applications of digital microfluidics in biomedical fields: An update of recent progress.

Significant breakthroughs have been made in digital microfluidic (DMF)-based technologies over the past decades. DMF technology has attracted great interest in bioassays depending on automatic microscale liquid manipulations and complicated multi-step processing. In this review, the recent advances of DMF platforms in the biomedical field were summarized, focusing on the integrated design and applications of the DMF system. Firstly, the electrowetting-on-dielectric principle, fabrication of DMF chips, and commercialization of the DMF system were elaborated. Then, the updated droplets and magnetic beads manipulation strategies with DMF were explored. DMF-based biomedical applications were comprehensively discussed, including automated sample preparation strategies, immunoassays, molecular diagnosis, blood processing/testing, and microbe analysis. Emerging applications such as enzyme activity assessment and DNA storage were also explored. The performance of each bioassay was compared and discussed, providing insight into the novel design and applications of the DMF technology. Finally, the advantages, challenges, and future trends of DMF systems were systematically summarized, demonstrating new perspectives on the extensive applications of DMF in basic research and commercialization.

Simultaneous desalination and molecular resource recovery from wastewater using an electrical separation system integrated with a supporting liquid membrane.

Separating molecular substances from wastewater has always been a challenge in wastewater treatment. In this study, we propose a new strategy for simultaneous desalination and selective recovery of molecular resources, by introducing a supported liquid membrane (SLM) with molecular selectivity into an asymmetric flow-electrode capacitive deionization. Salts and molecular substances in wastewater are removed after passing through the ion separation chamber and the molecular separation chamber, respectively. Faradaic reactions, i.e., the electrolysis of water with OH-, occurred in the electrochemical cathode electrode provides a sufficient and continuous chemical potential gradient for the cross-SLM transport of phenol (a model molecule substance). By optimizing the formulation of the liquid membrane and the pore size of the support membrane, we obtained the SLM with the best performance for separating phenol. In continuous experiment tests, the electrochemical membrane system showed stable separation performance and long-term stability for simultaneous salts removal and phenol (sodium phenol) recovery from wastewater. Finally, we demonstrate the potential application of this technology for the recovery of different carbon resources. Overall, the electrochemical system based on SLM is suitable for various wastewater treatment needs and provides a new approach for the recovery of molecular resources in wastewater.

Electro-enhanced metal-free peroxymonosulfate activator coupled with membrane-assisted process for simultaneous Ni-EDTA decomplexation and Ni ions recovery.

Electro-enhanced metal-free boron/peroxymonosulfate (B/PMS) system has demonstrated potential for efficient metal-organic complexes degradation in an eco-friendly way. However, the efficiency and durability of the boron activator are limited by associated passivation effect. Additionally, the lack of suitable methods utilizing in-situ recovery of metal ions liberated from decomplexation causes huge resource waste. In this study, B/PMS coupled with a customized flow electrolysis membrane (FEM) system is proposed to address above challenges with Ni-EDTA used as the model contaminant. Electrolysis is confirmed to remarkably promote the activation performance of boron towards PMS to efficiently generate •OH which dominated Ni-EDTA decomplexation in the anode chamber. It is revealed that the acidification near the anode electrode improves the stability of boron by inhibiting passivation layer growth. Under optimal parameters (10 mM PMS, 0.5 g/L boron, initial pH = 2.3, current density = 68.87 A/m2), 91.8% of Ni-EDTA could be degraded in 40 min, with a kobs of 6.25 × 10-2 min-1. As the decomplexation proceeds, nickel ions are recovered in the cathode chamber with little interference from the concentration of co-existing cations. These findings provide a promising and sustainable strategy for simultaneous metal-organic complexes removal and metal resources recovery.

From black water to flushing water: potential applications of chlorine-mediated indirect electrooxidation for ammonia removal.

Removing ammonia from black water is one of the most urgent issues before it can be recycled as flushing water. In this study, an electrochemical oxidation (EO) process with commercial Ti/IrO2-RuO2 anodes to treat black water could remove 100% of different concentrations of ammonia by adjusting the dosage of chloride. Through the relationship between ammonia, chloride, and corresponding the pseudo-first-order degradation rate constant (Kobs), we could determine the chloride dosage and predict the kinetics of ammonia oxidation based on initial ammonia concentration in black water. The optimal N/Cl molar ratio was 1:1.8. The difference between black water and the model solution in terms of ammonia removal efficiency and oxidation products was explored. A higher chloride dosage was beneficial for removing ammonia and shortening the treatment cycle, but it also led to the generation of toxic by-products. Especially HClO and ClO3- generated in black water were 1.2 and 1.5 times more than the synthesized model solution under 40 mA cm-2. Through SEM characterization of electrodes and repeated experiments, the electrodes always maintained a high treatment efficiency. These results demonstrated the potential of the electrochemical process as a treatment method for black water.

Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana.

BACKGROUND: MicroRNAs (miRNAs) and other epigenetic modifications play fundamental roles in all eukaryotic biological processes. DNA damage repair is a key process for maintaining the genomic integrity of different organisms exposed to diverse stresses. However, the reaction of miRNAs in the DNA damage repair process is unclear. RESULTS: In this study, we found that the simultaneous mutation of zinc finger DNA 3'-phosphoesterase (ZDP) and AP endonuclease 2 (APE2), two genes that play overlapping roles in active DNA demethylation and base excision repair (BER), led to genome-wide alteration of miRNAs. The transcripts of newly transcribed miRNA-encoding genes (MIRs) decreased significantly in zdp/ape2, indicating that the mutation of ZDP and APE2 affected the accumulation of miRNAs at the transcriptional level. In addition, the introduction of base damage with the DNA-alkylating reagent methyl methanesulfonate (MMS) accelerated the reduction of miRNAs in zdp/ape2. Further mutation of FORMAMIDOPYRIMIDINE DNA GLYCOSYLASE (FPG), a bifunctional DNA glycosylase/lyase, rescued the accumulation of miRNAs in zdp/ape2, suggesting that the accumulation of DNA damage repair intermediates induced the transcriptional repression of miRNAs. CONCLUSIONS: Our investigation indicates that the accumulation of DNA damage repair intermediates inhibit miRNAs accumulation by inhibiting MIR transcriptions.

Electric field-enhanced coupled with metal-free peroxymonosulfate activactor: The selective oxidation of nonradical species-dominated system.

Nowadays metal-free persulfate-based advanced oxidation processes (AOPs) have been intensively investigated, however, the catalysts are often too complex to fully consider their application potential. Conventional AOPs usually suffer from severe interference in real water matrix, thus, selective oxidation is practically and scientifically challenging as it could avoid unnecessary inputs of energy and possible secondary pollutants. In this study, a remarkably synergistic effect was achieved when conventional amorphous boron/peroxymonosulfate (Boron/PMS, 0.67 × 10-2 min-1) system was combined with electrolysis (E-Boron/PMS, 1.54 × 10-2 min-1) to degrade sulfamethoxazole (SMX). Evidenced by selectively quenching tests with kinetic evaluation, electron paramagnetic resonance (EPR), solvent-exchange experiment and electrochemical analysis, the dominated reactive oxygen species in E-Boron/PMS system tended to be 1O2, instead of the •OH and SO4•-. Mechanistic study unveiled that 1O2 was generated via accelerated PMS self-decomposition, triggered by interface alkalization and hydroxyl radicals transfer at the cathode interface. 1O2 is considered to be selective to the electron-rich organic compounds, thus E-Boron/PMS system was superior to conventional radical-dominated system (Boron/PMS) for SMX removal in the co-presence of common inorganic anions, showing the great merits of selective oxidation in nonradical system. These findings provided new insights into effective and selective oxidation of SMX via E-Boron/PMS system, which shed new light on the development of nonradical system.

Non-Covalent Bond-Regulated Solar Evaporation Modulator: Facilitative Hydration Domains Originated via a Homogeneous Polymeric Network.

The solar-driven evaporation technology provides a green alternative for solving water scarcity. However, it remains challenging to improve the steam conversion efficiency due to the difficulties in simultaneously coordinating light absorbance, water regulation, and thermal management for broadband solar evaporators. Here, an unconventional solar evaporative modulator material─ultra-interfacial adherent dimethyl sulfoxide polyvinyl alcohol (DMSO-PVA) hydrogel (DPH) was presented. The material is based on the regulation of the PVA-PVA intra- and PVA-water interchain hydrogen bonds by DMSO, which established an adaptive high-cross-linking and homogeneous network. The consequent ultra-thin hydrogel exploited an insulating polymer backbone and intracavity hydration domain to simultaneously improve the light absorption and thermal localization and activate the water molecule. As a proof-of-concept, under 1 sun illumination (1 kW m-2), a DPH-based graphene fiber membrane [ultra-thin hydrogel membrane (UHM)] achieved 97% light absorption, 2.33 kg m-2 h-1 water evaporation, and high salt-resistant evaporation (1.48 kg m-2 h-1 under 25 wt % brine). Compared to the pure graphene membrane, UHM increased the vaporization by 64%, decreased the heat diffusion by over 14-folds, and reduced the environmental heat loss by 2.6-folds. DPH possesses scalability and versatility in bridging nanoscale photothermal materials and solar evaporator geometric architecture and will facilitate the possibility of advanced solar thermal applications.

Precise manipulation of the charge percolation networks of flow-electrode capacitive deionization using a pulsed magnetic field.

Magnetic field is a simple and powerful means that enables controlled the transport of electrode particles in flow electrode capacitive deionization (FCDI). However, the magnetic particles are easily stripped from hybrid suspension electrodes and the precise manipulation of the charge percolation network remains challenging. In this study, a programmable magnetic field was introduced into the FCDI system to enhance the desalination performance and operational stability of magnetic FCDI, with core-shell magnetic carbon (MC) used as an alternative electrode additive. The results showed that the pulsed magnetic field (PMF) was more effective in enhancing the average salt removal rate (ASRR) compared to the constant magnetic field (CMF), with 51.6% and 67.7% enhancement, respectively, compared to the magnetic field-free condition. The outstanding advantage of the PMF lies in the enhancement in the trapping and mediating effects in the switching magnetic field, which keeps the concentration of the electrode particles near the current collector at a high level and greatly facilitates electron transport. In long-term operation (20,000 cycles), the pulsed magnetic FCDI achieved a stable desalinating rate of 0.4-0.68 µmol min-1 cm-2 and a charge efficiency of >96%. In brief, our study introduces a new approach for the precise manipulation of charge percolation networks of the suspension electrodes and provides insight into the charging mechanism of the magnetic FCDI.

Enhancing Brackish Water Desalination using Magnetic Flow-electrode Capacitive Deionization.

Flow-electrode capacitive deionization (FCDI) is viewed as a potential alternative to the current state-of-the-art electrodriven technology for the desalination of brackish water. However, the key shortcoming of the FCDI is still the discontinuous nature of the electrode conductive network, resulting in low electron transport efficiency and ion adsorption capacity. Here, a novel magnetic field-assisted FCDI system (termed magnetic FCDI) is proposed to enhance brackish water desalination, simply by using magnetic activated carbon (MAC) as flow electrodes. The results show that the assistance from the magnetic field enables a 78.9% - 205% enhancement in the average salt removal rate (ASRR) compared with that in the absence of a magnetic field, which benefits from the artificial manipulation of the flow electrode transport behavior. In long-term tests, the stable desalination performance of magnetic FCDI was also demonstrated with a stable ASRR of 0.70 µmol cm-2 min-1 and energy-normalized removed salt (ENRS) of 8.77 µmol J-1. In addition, magnetic field also enables the regeneration of the electrode particles from the concentrated electrolyte. In summary, the findings indicate that the magnetic FCDI is an energy-efficient and operation convenient technology for brackish water desalination.

Surface-mediated periodate activation by nano zero-valent iron for the enhanced abatement of organic contaminants.

Periodate (PI)-based advanced oxidation processes have recently received increasing attentions. Herein, PI was readily activated by nano zero-valent iron (nZVI) and subsequently led to the enhanced oxidation of organic contaminants, with the removal performance of sulfadiazine (SDZ) in the nZVI/PI process even higher than that in the nZVI/peroxydisulfate process under identical conditions. Kinetic experiments indicated that the decay of SDZ was susceptible to the dosage of nZVI and PI, but was barely affected by pH values (4.0-7.0) under buffered conditions, suggesting the promising performance of the nZVI/PI process in a relatively wide pH range. Selective degradation of contaminants and 18O-isotope labeling assays collectively demonstrated that iodate radical (•IO3), high-valent iron-oxo species (Fe(IV)) and hydroxyl radical (•OH) were responsible for the abatement of organic contaminants. More importantly, due to the relatively weak steric hindrance effect of PI, PI easily adsorbed on the surface of nZVI and no iron leaching was detected throughout the reaction, implying that PI activation induced by nZVI was a surface-mediated process. Besides, PI was not transformed into harmful reactive iodine species. This study proposed an environmental-friendly approach for PI activation and shed new lights on the PI-based processes.

Reduced cathodic scale and enhanced electrochemical precipitation of Ca2+ and Mg2+ by a novel fenced cathode structure: Formation of strong alkaline microenvironment and favorable crystallization.

Electrochemical precipitation is a promising technique for hardness abatement without the addition of external ions. However, the scale layer on cathode deteriorated the removal efficiency and limited the practical application. Herein, a fenced cathode structure was designed to prevent cathodic precipitation. The cathode was fenced by a crystallization-inducing material for separating the OH- production and crystallization processes. Precipitation on the cathode was confirmed to shift to the crystallization-inducing material, and the clean fenced cathode provided efficient long-term OH- production. At a current density of 40 A/m2, the Ca2+ or Mg2+ removal efficiency increased by 12.8% or 46.1%, respectively, compared to those of a traditional cathode. Thermodynamic equilibrium in synthetic water and mine water, mass transfer and the location of precipitation were analyzed to elucidate the electrochemical precipitation process. The enhanced mechanism was ascribed to the crystallization-inducing material, which remarkably promoted the crystallization process, and hindered OH- migration, thereby increased the pH of alkaline microenvironment. Notably, a recovery design was proposed to recover pure calcite and brucite from alkalinity-free wastewater. The design reveals a promising strategy for enhancing the crystallization process and reducing cathodic scale, also initiating a new research direction toward hardness removal.

Highly selective oxidation of organic contaminants in the RuIII-activated peroxymonosulfate process: The dominance of RuVO species.

Ruthenium (RuIII)-activated peroxymonosulfate (the RuIII/PMS process) is one of the most efficient PMS-based advanced oxidation processes for the abatement of organic contaminants. Here we interestingly found that phenyl methyl sulfoxide (PMSO) was significantly oxidized to phenyl methyl sulfone (PMSO2) in the RuIII/PMS process at the pH range of 3.0-6.0, with the conversion ratio of ΔPMSO to ΔPMSO2 was close to 100%, which favored the dominance of high-valent ruthenium-oxo species (RuVO) instead of the widely-recognized radicals (i.e, hydroxyl radical and sulfate radical). Scavenging experiments further indicated that RuVO was unreactive to tert-butyl alcohol, but could be scavenged by methanol and dimethyl sulfoxide. Besides, sulfamethoxazole, acetaminophen, carbamazepine, diclofenac, 2,4,6-trichlorophenol were readily degraded in the RuIII/PMS process, but atrazine, ibuprofen, benzoic acid and 4-nitrobenzoic acid were barely removed, suggesting the high selectivity of RuVO species. This study enriched the understandings on the mechanism of RuIII-mediated PMS activation and the nature of RuVO species.

Membrane-Current Collector-Based Flow-Electrode Capacitive Deionization System: A Novel Stack Configuration for Scale-Up Desalination.

The stack configuration in flow-electrode capacitive deionization (FCDI) has been verified to be an attractive and feasible strategy for scaling up the desalination process. However, challenges still exist when attempting to simultaneously improve the desalination scale and the cell configuration. Here, we describe a novel stack FCDI configuration (termed a gradient FCDI system) based on a membrane-current collector assembly, in which the charge neutralization enables the in situ regeneration of the flow electrodes in the single cycle operation, thereby realizing a considerable increase in the desalinating performance. By evaluating standardized metrics such as the salt rejection, productivity (P), average salt removal rate (ASRR), energy-normalized removed salt (ENRS), and TEE, the results indicated that the gradient FCDI system could be a performance-stable and energy-efficient alternative for scale-up desalination. Under optimal operating conditions (carbon content = 10 wt %, feed salinity = 3000 mg L-1, cell voltage = 1.2 V, and productivity = 56.7 L m-2 h-1), the robust desalination performance (ASRR = 1.07 µmol cm-2 min-1) and energy consumption (ENRS = 7.8 µmol J-1) of the FCDI system with a desalination unit number of four were verified at long-term operation. In summary, the stacked gradient FCDI system and its operation mode described here may be an innovative and promising strategy capable of enlarging the scale of desalination while realizing performance improvement and device simplification.

Selective recovery of phosphorus and urea from fresh human urine using a liquid membrane chamber integrated flow-electrode electrochemical system.

Phosphorus (P) extraction from human urine is a potential strategy to address global resource shortage, but few approaches are able to obtain high-quality liquid P products. In this study, we introduced an innovative flow-electrode capacitive deionization (FCDI) system, also called ion-capture electrochemical system (ICES), for selectively extracting P and N (i.e., urea) from fresh human urine simply by integrating a liquid membrane chamber (LMC) using a pair of anion exchange membrane (AEM). In the charging process, negatively charged P ions (i.e., HPO42- and H2PO4-) can be captured by acidic extraction solutions (e.g., solutions of HCl, HNO3 and H2SO4) on their way to the anode chamber, leading to the conversion of P ions to uncharged H3PO4, while other undesired ions such as Cl- and SO42- are expelled. Simultaneously, uncharged urea molecules remain in the urine effluent with the removal of salt. Thus, high-purity phosphoric acid and urea solutions can be obtained in the LMC and spacer chambers, respectively. The purification of P in an acidic environment is ascribed largely to the competitive migration and protonation of ions. The latter contributes ~27% for the selective capture of P. Under the optimal operating conditions (i.e., ratio of the urine volume to the HCl volume = 7:3, initial pH of the extraction solution = 1.43, current density = 20 A/m2 and threshold pH ~ 2.0), satisfactory recovery performance (811 mg/L P with 73.85% purity and 8.3 g/L urea-N with 81.4% extraction efficiency) and desalination efficiency (91.1%) were obtained after 37.5 h of continuous operation. Our results reveal a promising strategy for improving in selective separation and continuous operation via adjustments to the cell configuration, initiating a new research dimension toward selective ion separation and high-quality P recovery.

Deactivation of Caenorhabditis elegans nematodes in drinking water by PMS/UV-C: efficiency and mechanisms.

The occurrence and infestations of chlorine-resistant invertebrates in drinking water distributions have attracted concerns on water quality in China, making effective deactivation imperative. This study presents a novel strategy for nematode (Caenorhabditis elegans) deactivation using peroxymonosulfate (PMS)/UV-C. The results indicated that 100% deactivation efficiency was obtained under optimal conditions. An acidic pH and 0.25 mg/L Fe(II) were beneficial to the PMS/UV-C-triggered deactivation of nematodes. A mechanism study demonstrated that [Formula: see text] was activated by UV-C to produce ·OH and [Formula: see text], which resulted in oxidative stress and stimulated the occurrence of cell apoptosis, leading to nematode deactivation. The results reveal PMS/UV-C as an alternative to chlorination in water treatment plants (WTP) or an emergency application when chlorine-resistant invertebrates breed in a second-supply water tank is a promising strategy for disinfection. This approach possessed the advantages of avoiding the production of chlorine disinfection by-products (DBP) and greater efficacy of nematode deactivation. This work will provide ideas for on-going research efforts into chlorine-resistant invertebrate deactivation and eventually achieve the direct drinking of municipal tap water.

Enhanced Oxidation of Organic Contaminants by Iron(II)-Activated Periodate: The Significance of High-Valent Iron-Oxo Species.

Potassium periodate (PI, KIO4) was readily activated by Fe(II) under acidic conditions, resulting in the enhanced abatement of organic contaminants in 2 min, with the decay ratios of the selected pollutants even outnumbered those in the Fe(II)/peroxymonosulfate and Fe(II)/peroxydisulfate processes under identical conditions. Both 18O isotope labeling techniques using methyl phenyl sulfoxide (PMSO) as the substrate and X-ray absorption near-edge structure spectroscopy provided conclusive evidences for the generation of high-valent iron-oxo species (Fe(IV)) in the Fe(II)/PI process. Density functional theory calculations determined that the reaction of Fe(II) with PI followed the formation of a hydrogen bonding complex between Fe(H2O)62+ and IO4(H2O)-, ligand exchange, and oxygen atom transfer, consequently generating Fe(IV) species. More interestingly, the unexpected detection of 18O-labeled hydroxylated PMSO not only favored the simultaneous generation of ·OH but also demonstrated that ·OH was indirectly produced through the self-decay of Fe(IV) to form H2O2 and the subsequent Fenton reaction. In addition, IO4- was not transformed into the undesired iodine species (i.e., HOI, I2, and I3-) but was converted to nontoxic iodate (IO3-). This study proposed an efficient and environmental friendly process for the rapid removal of emerging contaminants and enriched the understandings on the evolution mechanism of ·OH in Fe(IV)-mediated processes.

Scale-up desalination: Membrane-current collector assembly in flow-electrode capacitive deionization system.

Salt removal from seawater/wastewater using flow-electrode capacitive deionization (FCDI) is of particular interest, but scale-up desalination is limited by low water production, high energy consumption and complex cell configuration. In this study, an innovative FCDI system is described that uses integrated desalination modules equipped with membrane-current collector (MCC) assembly, and thereby named as MCC-FCDI system. A single desalination module design provides an average salt removal rate (ASRR, 0.3 - 0.44 µmol/(cm2·min)) close to that of the classic FCDI system (with a graphite current collector design), but the design requires a much lower infrastructure investment, device size and energy cost. More importantly, our design enables simultaneous operation of multiple modules in the shared flow-electrode tank, easily realizing scale-up desalination. Evidence is provided by the results of the multi-module operation: multi-modules isolated closed-cycle (MICC) and multi-modules short-circuited closed-cycle (MSCC). For instance, the MICC configuration showing nearly twice the desalination performance over ~ 50 h of operation compared to that of the single ICC operation. The results indicated that in addition to making the device suitable for practical application, the Ti-mesh MCC with a woven network enables the flow electrode to achieve substantial ion adsorption capacity due to the efficient update of fresh carbon particles. In short, the results of this study showed that MCC-FCDI is a promising desalination system for scale-up applications, providing a new reference and guidance for device design.

Can flow-electrode capacitive deionization become a new in-situ soil remediation technology for heavy metal removal?

In this study, we present a novel soil electrochemical remediation technology (called S-FCDI), which is based on flow-electrode capacitive deionization (FCDI), for Cd removal from kaolin while under continuous operation mode. The results demonstrated that Cd can be effectively removed from kaolin with reasonable energy consumption and minimal macroelement loss. The carboxylic (OOH) functional groups on the surface of activated carbon (AC) facilitated the transfer of Cd from kaolin onto carbon surface. A stable acidic environment, which is advantageous for continuous Cd desorption, was achieved as a result of the balance between H+ generation and transmembrane migration. Once these net negative charges on the particle were eliminated or reversed, the adsorbed Cd could be released easily and driven in concentrated stream by electrostatic repulsion. Under the optimal operating conditions (i.e., carbon =50 g/L, j = 3.47 A/m2, pHi = 3.2, [NaCl]a =8.6 mmol/L), more than 80 % Cd was removed from (200 g) kaolin after continuous 19 h operation at a relatively low electricity consumption of 22.7 kW h/kg Cd and a limited Al loss of 0.06 wt‰. These results from this work demonstrated that S-FCDI could be an alternative soil electrochemical remediation technology for heavy metal removal with low soil damage.