Behavioral impulse and time pressure jointly influence intentional inhibition: evidence from the Free Two-Choice Oddball task.

Intentional inhibition is a crucial component of self-regulation, yet it is under-researched, because it is difficult to study without external stimuli or overt behaviors. Although Free-Choice tasks have been developed, it remains unclear how two key design features (i.e., behavioral impulse and time pressure) affect their sensitivity to intentional inhibition. To investigate this, the present study developed a Free Two-Choice Oddball task, which generated both an inhibition rate index and a response time (RT) index. Two experiments were conducted to systematically manipulate the ratio of the reactive standard to oddball trials and reaction time limit, inducing diverse behavioral impulses and different time pressures. The following findings were obtained from the critical Free-Choice trials. In the equal ratio condition, participants demonstrated comparable RTs for both the standard and oddball responses. In the moderate-ratio condition, participants exhibited longer RTs for the oddball than standard responses under low- but not high-time pressure. In the high-ratio condition, while RTs for the oddball responses were longer than those for the standard responses under both the high- and low-time pressures, participants displayed a decreased inhibition rate under the high-time pressure compared to the low-time pressure. Finally, participants exhibited a reduced inhibition rate in the high-ratio condition compared to the moderate-ratio condition. Together, these findings suggest that Free-Choice tasks can reflect intentional inhibition under specific conditions, and intentional inhibition is susceptible to both behavioral impulse and time pressure, while also establishing the theoretical and methodological foundations for subsequent research.

Childhood trauma, intraindividual reaction time variability, baseline respiratory sinus arrhythmia, and perceived relapse tendency among males with substance use disorders.

Background: People with substance use disorders (SUDs) who have experienced serious childhood trauma may have executive function impairments contributing to relapse. Baseline respiratory sinus arrhythmia (RSA) reflects physiological regulation capacity, which has been found to buffer the negative effects of childhood trauma. Baseline RSA has also been found to be related to intraindividual reaction time variability (IIRTV), which is an index of executive function.Objectives: The present study examined the relationship between childhood trauma and perceived relapse tendency, the mediation role of IIRTV, and the moderation role of baseline RSA.Methods: The study is cross-sectional, a total of 110 males with SUDs participated (Mage = 46.45 years, SD = 11.24). The Childhood Trauma Questionnaire and Intention to Rehabilitate Questionnaire were used to assess childhood trauma and perceived relapse tendency, the two-choice oddball task was used to measure IIRTV, and electrocardiogram (ECG) data were collected to obtain baseline RSA.Results: IIRTV mediated the relationship between childhood trauma and perceived relapse tendency (Coeff = 0.049, Boot CI [0.004, 0.121]); interaction of childhood trauma and Baseline RSA negatively influences IIRTV (ß = -0.208, t = -2.022, p = .046).Conclusion: The results suggest that males with SUDs who have experienced serious childhood trauma may have executive function impairments that contribute to relapse, and baseline RSA may buffer the negative effect of childhood trauma on IIRTV. These findings suggest that the prevention of relapse through cognitive enhancement can be complemented by the enhancement of physiological regulation.

Scarcity mindset impairs distractor suppression but not target enhancement in selective attention: Evidence from ERPs.

A scarcity mindset, induced by a perception of material scarcity, profoundly influences how individuals allocate their attention. Selective attention, a crucial cognitive control process enabling individuals to focus on relevant information while ignoring distractions, holds significant importance in both professional and personal domains. However, the specific impact of a scarcity mindset on selective attention and its underlying mechanisms remains uncertain. The objective of this study is to examine the specific impact of a scarcity mindset on selective attention using an experimental approach. Participants were subjected to either scarcity or neutral conditions, and their brain activity was measured using Electroencephalography (EEG) while performing a visual search task. The task involved identifying the direction of a target while disregarding a distractor, which was presented either laterally or in conjunction with the target along the vertical midline. The findings of our study indicate that individuals with a scarcity mindset displayed compromised selective attention, as evidenced by longer response times and weaker N2pc amplitudes in EEG recordings. Notably, distractor suppression was compromised, as evidenced by diminished Pd amplitudes, whereas the enhancement of targets remained relatively unaltered, as indicated by similar Nt amplitudes. These findings highlight a reduced ability to filter out irrelevant information in individuals with a scarcity mindset, thereby holding significant implications for social policies and practices.

A sheep pangenome reveals the spectrum of structural variations and their effects on tail phenotypes.

Structural variations (SVs) are a major contributor to genetic diversity and phenotypic variations, but their prevalence and functions in domestic animals are largely unexplored. Here we generated high-quality genome assemblies for 15 individuals from genetically diverse sheep breeds using Pacific Biosciences (PacBio) high-fidelity sequencing, discovering 130.3 Mb nonreference sequences, from which 588 genes were annotated. A total of 149,158 biallelic insertions/deletions, 6531 divergent alleles, and 14,707 multiallelic variations with precise breakpoints were discovered. The SV spectrum is characterized by an excess of derived insertions compared to deletions (94,422 vs. 33,571), suggesting recent active LINE expansions in sheep. Nearly half of the SVs display low to moderate linkage disequilibrium with surrounding single-nucleotide polymorphisms (SNPs) and most SVs cannot be tagged by SNP probes from the widely used ovine 50K SNP chip. We identified 865 population-stratified SVs including 122 SVs possibly derived in the domestication process among 690 individuals from sheep breeds worldwide. A novel 168-bp insertion in the 5' untranslated region (5' UTR) of HOXB13 is found at high frequency in long-tailed sheep. Further genome-wide association study and gene expression analyses suggest that this mutation is causative for the long-tail trait. In summary, we have developed a panel of high-quality de novo assemblies and present a catalog of structural variations in sheep. Our data capture abundant candidate functional variations that were previously unexplored and provide a fundamental resource for understanding trait biology in sheep.

Personal relative deprivation impairs the ability to inhibit impulsive responses: an exploratory ERP study.

Evidence indicates that personal relative deprivation (PRD) can lead to various impulsive behaviors. Given that impulsive behaviors are usually caused by a failure to exert cognitive control, the purpose of this study was to explore whether PRD affects the ability to exert cognitive control on behavior. Forty-six healthy participants were randomly assigned to PRD or non-PRD group. Participants of the PRD group were told their income would lie below the Chinese average. While their electrophysiological responses were recorded, they underwent a Go/No-Go task simultaneously assessing the ability to detect response conflict and inhibit the predominant response. We found that the individuals with induced PRD show diminished ability to inhibit predominant response. We suggest this is because PRD-related concerns consume cognitive resources, leaving less for other tasks. However, we also found that individuals with induced PRD show enhanced ability to detect conflict. This might be because that individuals with induced PRD were sensitive to potentially threatening information (high-conflict No-Go trials) and they can detect conflict with less cognitive resources. These findings may facilitate future attempts to design interventions for relatively deprived individuals to manage their impulsive behavior.

Complete mitochondrial genomes reveal the phylogenetic relationships of Duoma with eight representative sheep breeds and two ovis species.

The Ovis aries Duoma breed is one of the most important alpine grassland-type sheep breeds in China. In this current study, we first reported the complete mitochondrial genome of Duoma sheep. Mitochondrial genome is 16,618 bp in length and exhibits the same typical structure as the other published sheep breeds. The genome contains 37 genes (22 tRNA, two rRNA, and 13 protein-coding genes (PCGs)) and a control region (D-loop region). Phylogenetic analysis shows that the Duoma sheep breed is closer to Ganjia sheep. Our findings will help the further evolution and conservation studies of Duoma sheep.

Trust violations affect the emotional working memory updating: An event-related brain potential study.

Previous studies found that trust violations impaired the ability of working memory (WM) updating, however, these studies did not consider the emotional context in the WM updating. Individuals who experience trust violations have the characteristics of negative bias and enhanced negative emotion. Thus it is necessary to explore how emotional contexts moderate the relationship between trust violations and WM updating. In this study, the trust game was used to manipulate trust violations. Fifty-three participants performed the emotional two-back task while event-related potentials were recorded. Results showed that compared to the control group, the violation group had smaller P2 and P3 amplitudes both in emotional and nonemotional contexts and larger N2 amplitudes in the emotional contexts. There were no significant differences between the two groups on the behavioral data. These results suggest that trust violations result in the inefficient allocation of attention in the early attention (P2) and updating maintenance stages (P3) regardless of the emotional type of the material. Trust violations also improve the abilities of response inhibition, conflict monitoring, or sequential match (N2) when processing emotional material, which may play a compensatory role to maintain a level of behavioral performance comparable to the control group. Together, trust violations affect the sub-processes underlying emotional WM updating differently, and these influences are not valence specific.

Social exclusion modulates neural dynamics of monetary and social reward processing in young adult females.

Evidence suggests that social exclusion increases one's sensitivity to monetary and social rewards. However, whether and how social exclusion modulates the neural dynamics of reward processing remains unknown. The current study aimed to address this gap by systematically investigating the differential influences of social exclusion on various stages of monetary and social reward processing. Forty-five female participants were recruited, and the Cyberball game was used to manipulate social exclusion. To disentangle the anticipatory and consummatory stages of monetary and social reward processing, we recorded event-related potentials during two incentive delay tasks, one with a monetary reward and one with a social reward. The results showed that during the anticipatory stage, a larger contingent negative variation was observed for the exclusion group than for the inclusion group, regardless of reward type. During the consummatory stage, although the reward-related positivity was larger in the exclusion group than in the inclusion group, this difference was only observed for the social, and not monetary, reward feedback. These findings advance our understanding of the relationship between social exclusion and reward processing and suggest that while social exclusion might exert comparable enhancement effect for monetary and social reward processing during the anticipatory stage, it exerts a specific enhancement effect for social reward processing during the consummatory stage.

Deep Sequencing of the Rat MCAO Cortexes Reveals Crucial circRNAs Involved in Early Stroke Events and Their Regulatory Networks.

Circular RNAs (circRNAs) are highly enriched in the central nervous system and significantly involved in a range of brain-related physiological and pathological processes. Ischemic stroke is a complex disorder caused by multiple factors; however, whether brain-derived circRNAs participate in the complex regulatory networks involved in stroke pathogenesis remains unknown. Here, we successfully constructed a cerebral ischemia-injury model of middle cerebral artery occlusion (MCAO) in male Sprague-Dawley rats. Preliminary qualitative and quantitative analyses of poststroke cortical circRNAs were performed through deep sequencing, and RT-PCR and qRT-PCR were used for validation. Of the 24,858 circRNAs expressed in the rat cerebral cortex, 294 circRNAs were differentially expressed in the ipsilateral cerebral cortex between the MCAO and sham rat groups. Cluster, GO, and KEGG analyses showed enrichments of these circRNAs and their host genes in numerous biological processes and pathways closely related to stroke. We selected 106 of the 294 circRNAs and constructed a circRNA-miRNA-mRNA interaction network comprising 577 sponge miRNAs and 696 target mRNAs. In total, 15 key potential circRNAs were predicted to be involved in the posttranscriptional regulation of a series of downstream target genes, which are widely implicated in poststroke processes, such as oxidative stress, apoptosis, inflammatory response, and nerve regeneration, through the competing endogenous RNA mechanism. Thus, circRNAs appear to be involved in multilevel actions that regulate the vast network of multiple mechanisms and events that occur after a stroke. These results provide novel insights into the complex pathophysiological mechanisms of stroke.

Comparative Transcriptome Analysis of Key Genes and Pathways Activated in Response to Fat Deposition in Two Sheep Breeds With Distinct Tail Phenotype.

Fat tail in sheep presents a valuable energy reserve that has historically facilitated adaptation to harsh environments. However, in modern intensive and semi-intensive sheep industry systems, breeds with leaner tails are more desirable. In the present study, RNA sequencing (RNA-Seq) was applied to determine the transcriptome profiles of tail fat tissues in two Chinese sheep breeds, fat-rumped Altay sheep and thin-tailed Xinjiang fine wool (XFW) sheep, with extreme fat tail phenotype difference. Then the differentially expressed genes (DEGs) and their sequence variations were further analyzed. In total, 21,527 genes were detected, among which 3,965 displayed significant expression variations in tail fat tissues of the two sheep breeds (P < 0.05), including 707 upregulated and 3,258 downregulated genes. Gene Ontology (GO) analysis disclosed that 198 DEGs were related to fat metabolism. In Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, the majority of DEGs were significantly enriched in "adipocytokine signaling," "PPAR signaling," and "metabolic pathways" (P < 0.05); moreover, some genes were involved in multiple pathways. Among the 198 DEGs, 22 genes were markedly up- or downregulated in tail fat tissue of Altay sheep, indicating that these genes might be closely related to the fat tail trait of this breed. A total of 41,724 and 42,193 SNPs were detected in the transcriptomic data of tail fat tissues obtained from Altay and XFW sheep, respectively. The distribution of seven SNPs in the coding regions of the 22 candidate genes was further investigated in populations of three sheep breeds with distinct tail phenotypes. In particular, the g.18167532T/C (Oar_v3.1) mutation of the ATP-binding cassette transporter A1 (ABCA1) gene and g.57036072G/T (Oar_v3.1) mutation of the solute carrier family 27 member 2 (SLC27A2) gene showed significantly different distributions and were closely associated with tail phenotype (P < 0.05). The present study provides transcriptomic evidence explaining the differences in fat- and thin-tailed sheep breeds and reveals numerous DEGs and SNPs associated with tail phenotype. Our data provide a valuable theoretical basis for selection of lean-tailed sheep breeds.

Identification of differentially expressed miRNAs in serum extracellular vesicles (EVs) of Kazakh sheep at early pregnancy.

MiRNAs-containing extracellular vesicles (EVs) possess the unique function of mediating intercellular communication and participating in many biological processes such as post-transcriptional gene regulation of embryo implantation and placental development. In the present study, Illumina small-RNA sequencing was used to identify differentially expressed (DE) miRNAs in serum EVs of pregnant (P) and non-pregnant (NP) Kazakh sheep at Day 17 from mating. The specifically and differentially expressed miRNAs at early pregnancy in sheep were verified by using RT-PCR. The target genes of DE miRNAs were predicted by bioinformatics software, and the functional and pathway enrichment analysis was performed on Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) terms. A total of 562 miRNAs (210 novel miRNAs) were identified by sequencing, of which 57 miRNAs were differentially expressed, 49 were up-regulated, 8 were down-regulated and 22 novel miRNAs were specifically expressed in the pregnant sheep. Eight highly expressed known miRNA (miR-378-3p, miR-320-3p, miR-22-3p, let-7b, miR-423-3p, miR-221, miR-296-3p, miR-147-3p) in pregnant group were down-regulated in the control group. miRNAs-containing pregnancy-related terms and regulatory pathways regulation were enriched using both GO and KEGG analyses. Moreover, we also envisioned a miRNA-mRNA interaction network to understand the function of miRNAs involved in the early pregnancy serum regulatory network. The results of RT-PCR verification confirmed the reliability of small-RNA sequencing. Among them, miR-22-3p and miR-378-3p were significantly differentially expressed (DE) between pregnant sheep and non-pregnant group (p <  0.01). The site at which oar-miR-22-3p binds MAPK3 was determined with a dual-luciferase system. This is the first integrated analysis of the expression profiles of EV-miRNAs and their targets during early pregnancy in ewes. These data identify key miRNAs that influence the implantation of sheep in the early stage of pregnancy, and provide theoretical basis for further molecular regulatory mechanisms research.

Personal relative deprivation impairs ability to filter out threat-related distractors from visual working memory.

The literature has indicated that personal relative deprivation (PRD) results in anxiety disorders. Given that some cognitive models propose that attention bias toward a threat causes and maintains anxiety, relatively deprived individuals may have difficulty gating threat from working memory. To test this hypothesis, this study investigated the influence of PRD on the filtering ability of happy, angry, and neutral facial distractors from visual working memory using electroencephalography (EEG). Participants were randomly assigned to a PRD (n = 24) or a non-PRD group (n = 24). Filtering ability was reflected by comparing the contralateral delay activity (CDA) amplitude for one-target, one-target-one-distractor, and two-targets conditions. The CDA was measured as the difference in mean amplitudes between activity in the hemispheres contralateral and ipsilateral to the to-be-remembered information. Results indicated that individuals in the PRD group showed a reduced ability to filter out neutral and angry facial distractors, as reflected by similar CDA amplitudes for one-target-one-distractor and two-targets conditions for both angry and neutral distractors in the PRD group. However, PRD did not impair the ability to filter out happy facial distractors, as reflected by similar CDA amplitudes for one-target-one-distractor and one-target conditions for happy distractors in the PRD group. As neutral faces might then be taken as potentially threatening information by relatively deprived individuals, these results support the hypothesis that relatively deprived individuals might have difficulty filtering out threat-related information.

Bottom-up perceptual salience and top-down retro-cues concurrently determine state in visual working memory.

Recent studies have demonstrated that in visual working memory (VWM), only items in an active state can guide attention. Further evidence has revealed that items with higher perceptual salience or items prioritised by a valid retro-cue in VWM tend to be in an active state. However, it is unclear which factor (perceptual salience or retro-cues) is more important for influencing the item state in VWM or whether the factors can act concurrently. Experiment 1 examined the role of perceptual salience by asking participants to hold two features with relatively different perceptual salience (colour vs. shape) in VWM while completing a visual search task. Guidance effects were found when either colour or both colour and shape in VWM matched one of the search distractors but not when shape matched. This demonstrated that the more salient feature in VWM can actively guide attention, while the less salient feature cannot. However, when shape in VWM was cued to be more relevant (prioritised) in Experiment 2, we found guidance effects in both colour-match and shape-match conditions. That is, both more salient but non-cued colour and less salient but cued shape could be active in VWM, such that attentional selection was affected by the matching colour or shape in the visual search task. This suggests that bottom-up perceptual salience and top-down retro-cues can jointly determine the active state in VWM.

Neuritin-overexpressing transgenic mice demonstrate enhanced neuroregeneration capacity and improved spatial learning and memory recovery after ischemia-reperfusion injury.

Acute ischemia-reperfusion (IR)-induced brain injury is further exacerbated by a series of slower secondary pathogenic events, including delayed apoptosis due to neurotrophic factor deficiency. Neuritin, a neurotrophic factor regulating nervous system development and plasticity, is a potential therapeutic target for treatment of IR injury. In this study, Neuritin-overexpressing transgenic (Tg) mice were produced by pronuclear injection and offspring with high overexpression used to generate a line with stable inheritance for testing the neuroprotective capacity of Neuritin against transient global ischemia (TGI). Compared to wild-type mice, transgenic mice demonstrated reduced degradation of the DNA repair factor poly [ADP-ribose] polymerase 1 (PARP 1) in the hippocampus, indicating decreased hippocampal apoptosis rate, and a greater number of surviving hippocampal neurons during the first week post-TGI. In addition, Tg mice showed increased expression of the regeneration markers NF-200, synaptophysin, and GAP-43, and improved recovery of spatial learning and memory. Our findings exhibited that the window of opportunity of neural recovery in Neuritin transgenic mice group had a tendency to move ahead after TGI, which indicated that Neuritin can be used as a potential new therapeutic strategy for improving the outcome of cerebral ischemia injury.

Flexible adjustment of the effective connectivity between the fronto-parietal and visual regions supports cognitive flexibility.

Evidence indicates the significance of the fronto-parietal regions and inertia sensory processing from previous trials in cognitive flexibility. However, how flexible cognitive performance is achieved by causal interactions between cortical regions, particularly those between the fronto-parietal and stimulus processing regions, remains unknown. In the current study, the effective connectivity between the fronto-parietal and visual regions was examined in the context of a cued task-switching paradigm. We found that the fronto-parietal and visual cortex were differently activated during task transition (task repeat and task switch). Importantly, dynamic causal modeling (DCM) analysis revealed that task transition could modulate the effective connectivity between the fronto-parietal and visual cortex: task repeat decreased, while task switch enhanced, the coupling between the posterior parietal cortex (PPC) and the visual cortex. Furthermore, Granger causality analysis (GCA) showed that the dominant direction of influence was from the fronto-parietal regions to the visual cortex. Finally, individual differences in the top-down influence from the PPC to the visual cortex and the corresponding neural adjustment (task switch‒task repeat) was negatively associated with the behavioral switch cost. Our findings suggest that the interaction between the fronto-parietal and stimulus processing regions, particularly the top-down influence from the PPC to the visual cortex, is of particular importance in flexible cognitive performance.

Social exclusion modulates dual mechanisms of cognitive control: Evidence from ERPs.

Many studies have investigated how social exclusion influences cognitive control but reported inconsistent findings. Based on the dual mechanisms of control framework, this study investigated how social exclusion influences proactive and reactive modes of control (Experiment 1) and the underlying mechanisms (Experiment 2). The Cyberball game was used to manipulate social exclusion. Eighty-six female participants (about 40 for each experiment) performed cognitive control tasks while event-related potentials were recorded. In Experiment 1, an AX Continuous Performance Task (AX-CPT) was adopted to differentiate between proactive and reactive control. Results showed that social exclusion weakened proactive control but enhanced reactive control, as reflected by the weaker proactive control indicators (i.e., P3b and CNV), but strengthened reactive control indicators (accuracy and N2) in excluded individuals. More importantly, in Experiment 2, through varying in whether task cues were available before or after target onset in a cued-flanker task, we further manipulated the possibility of engaging proactive control, and found the weakened proactive control could be attributed to both impaired cognitive ability and lowered motivation to engage proactive control in excluded individuals. Together, these results provide insight on how social exclusion influences cognitive control and suggest promising implications for designing effective interventions to relieve the negative impact of social exclusion.

MHC-DRB1 exon 2 polymorphism and its association with mycoplasma ovipneumonia resistance or susceptibility genotypes in sheep.

Major histocompatibility complex (MHC) polymorphisms are associated with animal and human diseases. However, only a few studies have reported an association between MHC polymorphisms and mycoplasma ovipneumonia (MO). In the present study, three resistance/susceptibility genotypes associated with MO were identified by polymerase chain reaction-restriction fragment length polymorphism genotyping, assessing the clinical and pathological features, and examining the immune factors. The current results showed that MvaI bb and HaeIII ee were dominant genotypes in the susceptible Hu population, while MO-resistant populations, Dorper and D 9 H hybrids, were dominated by the MvaI cc and HaeIII dd genotypes, suggesting that MvaI cc and HaeIII dd genotypes might be associated with the trait of MO resistance. Further, the clinical symptoms and pathological morphology in the susceptibility group infected with MO were more severe than those in the resistant groups infected similarly. The data on the changes in the immune factor responses were utilized to deduce the molecular mechanism underlying the MO resistance/susceptibility. The results showed that the susceptible genotypes promote the inflammatory responses by inducing a high expression of TNFa, IFNc, IL-4, IL-6, and IL-1b, while the resistant genotypes inhibit the inflammatory response by increasing the expression of IL-2 and IL-10 significantly. This finding would provide the theoretical guidance for propagating sheep breeds that are highly resistant to MO.

Multiple representations in visual working memory simultaneously guide attention: The type of memory-matching representation matters.

Whether multiple visual working memory (VWM) representations can simultaneously become active templates to guide attention is controversial. The single-item-template hypothesis argues that only one VWM representation can be active at a time, whereas the multiple-item-template hypothesis argues that multiple VWM templates can simultaneously guide attention. The present study examined the two hypotheses in three (out of four) experiments, using three different types of memory objects: Experiment 1: shapes; Experiment 2: colors; and Experiment 3: colored shapes. Participants were required to hold one (memory-1) or two objects (memory-2) in VWM while performing a tilted line search task. Zero (match-0), one (match-1), or two (match-2) memory stimuli reappeared as distractors in the search array. Guidance effects were found for each type of memory stimuli. More importantly, the guidance effect for memory-2/match-2 trials was significantly larger than that for memory-2/match-1 and memory-1/match-1 trials when holding two colors or two colored shapes in VWM, which is in line with the multiple-item-template hypothesis. However, the pattern of simultaneous guidance effect is not perfectly found for two memory shapes, which may indicate that a reliable simultaneous guidance effect from two representations in VWM can be observed only when the memory-matching stimuli is more effective in guiding attention. Experiment 4 directly compared the guidance effect induced by feature-based matches (partial matching) with the guidance effect induced object-based matches (complete matching) in memory-set-size 2. Reliable guidance effects in match-1 and match-2 trials for object-based matches but not for feature-based matches confirmed the crucial role of the type of memory-matching stimuli in guiding attention.

Electrocortical evidence for the time course processes of attentional bias toward infant faces.

Previous behavioral studies have demonstrated that adults show initial orientation toward neutral infant faces compared to neutral adult faces. However, it is unclear whether the initial orientation toward infant faces is sustained and whether attentional bias toward infant faces exists under different emotional valences. In the present study, event-related potentials were recorded while nulliparous women performed a dot-probe task to identify the time course processes involved in attentional bias toward infant faces. Three face pair types were used in the task; each face pair type contained one infant facial expression (happy, neutral, or sad) and one adult facial expression with the same emotional valence. An early N2pc was observed for each face pair type, which suggests that there was initial orientation toward infant faces irrespective of emotional valence. The absence of sustained posterior contralateral negativity in all face pair types indicated that attentional orientation was not sustained at the later stage of attentional selection. In addition, an inverted late N2pc was observed for the happy face pairs and may reflect a "happy superiority effect" in attentional selection processing.

Comparative Proteome Analysis Reveals Lipid Metabolism-Related Protein Networks in Response to Rump Fat Mobilization.

Altay is a typical fat-tailed sheep breed displaying the unique ability to rapidly mobilize fat, which is vital for maintaining a normal metabolism that facilitates its survival in lengthy winter conditions. However, the physiological, biochemical, and molecular mechanisms underlying fat mobilization remain to be elucidated. In this study, the monitoring of rump fat adipocyte sizes disclosed a positive correlation between cell size and fat deposition ability. In addition, we subjected sheep to persistent starvation to imitate the conditions that trigger rump fat mobilization and screened 112 differentially expressed proteins using the isobaric peptide labeling approach. Notably, increased secretion of leptin and adiponectin activated the key fat mobilization signaling pathways under persistent starvation conditions. Furthermore, the upregulation of resistin (RETN), heat-shock protein 72 (HSP72), and complement factor D (CFD) promoted lipolysis, whereas the downregulation of cell death-inducing DFFA-like effector C (CIDEC) inhibited lipid droplet fusion, and the increase in HSP72 and apolipoprotein AI (Apo-AI) levels activated the body's stress mechanisms. The synergistic actions of the above hormones, genes, and signaling pathways form a molecular network that functions in improving the adaptability of Altay sheep to extreme environments. Our findings provide a reference for elucidating the complex molecular mechanisms underlying rump fat mobilization.