Endothelium-specific deletion of p62 causes organ fibrosis and cardiac dysfunction.

BACKGROUND: The autophagy adapter SQSTM1/p62 is crucial for maintaining homeostasis in various organs and cells due to its protein-protein interaction domains and involvement in diverse physiological and pathological processes. Vascular endothelium cells play a unique role in vascular biology and contribute to vascular health. METHODS: Using the Cre-loxP system, we generated mice with endothelium cell-specific knockout of p62 mediated by Tek (Tek receptor tyrosine kinase)-cre to investigate the essential role of p62 in the endothelium. In vitro, we employed protein mass spectrometry and IPA to identify differentially expressed proteins upon knockdown of p62. Immunoprecipitation assays were conducted to demonstrate the interaction between p62 and FN1 or LAMC2 in human umbilical vein endothelium cells (HUVECs). Additionally, we identified the degradation pathway of FN1 and LAMC2 using the autophagy inhibitor 3-methyladenine (3-MA) or proteasome inhibitor MG132. Finally, the results of immunoprecipitation demonstrated that the interaction between p62 and LAMC2 was abolished in the PB1 truncation group of p62, while the interaction between p62 and FN1 was abolished in the UBA truncation group of p62. RESULTS: Our findings revealed that p62 Endo mice exhibited heart, lung, and kidney fibrosis compared to littermate controls, accompanied by severe cardiac dysfunction. Immunoprecipitation assays provided evidence of p62 acting as an autophagy adapter in the autophagy-lysosome pathway for FN1 and LAMC2 degradation respectively through PB1 and UBA domain with these proteins rather than proteasome system. CONCLUSIONS: Our study demonstrates that defects in p62 within endothelium cells induce multi-organ fibrosis and cardiac dysfunction in mice. Our findings indicate that FN1 and LAMC2, as markers of (EndoMT), have detrimental effects on HUVECs and elucidate the autophagy-lysosome degradation mechanism of FN1 and LAMC2.

[Detection of anaerobes and drug sensitivity from the periodontal pockets of patients with combined periodontal-endodontic lesions].

PURPOSE: To investigate the correlation between the composition of bacterial flora isolated from deep periodontal pockets with combined periodontal-endodontic lesions, and to test the antibiotic susceptibility of anaerobes. METHODS: Microbial samples were taken from 82 deep periodontal pockets with combined periodontal-endodontic lesions using adequate techniques, and divided into two groups: endo-perio group and perio-endo group. Antibiotic susceptibility of cultivable anaerobes were tested with agar dilution method. RESULTS: Eighty-six cultivable anaerobes were isolated, including Streptococcus spp, Prevotella spp, Actinomycetes spp, Veillonella spp, Fusobacterium spp and etc. 53.4% were gram-positive anaerobes, while 46.51% were gram-negative anaerobes. The detection rate of anaerobes in endo-perio group and that in perio-endo group had significant difference. Most isolated anaerobes were sensitive to six drugs. The resistance rates of penicillin, clindamycin, metronidazole were 6.8%, 13.9% and 2.2%. CONCLUSIONS: There is a close relationship between anaerobes and combined periodontal-endodontic lesions. Drug sensitive susceptibility should be detected prior to clinical treatment of combined periodontal-endodontic lesions.

[Clinical effect of pit and fissure sealant used in combination with different etching adhesive and 3M-Z350 flowable resin on young permanent teeth].

PURPOSE: To compare the effect of different etching adhesive and flowable resin in deep pits and fissures of young permanent molars sealant technique. METHODS: Children aged 6-8 years old were included in the study. The split mouth design was used. The first permanent molars were randomly assigned into 2 experimental groups:A and B.The teeth in group A were sealed with flowable resin and self-etching adhesive,while the teeth in group B were sealed with flowable resin and phosphate acid system. The children were followed up every year, and the sealants were observed and evaluated. SPSS13.0 software package was used for statistical analysis. RESULTS: After 1 year, 2 and 3 years,the retention rate of group A was significantly lower than that of group B(P<0.05),while group A had more caries than group B(P<0.05) except at 1-year. CONCLUSION: Self-etching adhesive and flowable resin doesn't have excellent clinical effect on caries prevention.

[A preliminary analysis of the ratio between the upper and lower anterior teeth].

PURPOSE: The widths of anterior teeth are measured and compared to see if there was any specific ratio between the anterior teeth, and to provide some referential data for clinical application in esthetic dentistry. METHODS: Gypsum casts of the maxillary and mandibular arches were made by traditional ways. With the help of dividers and vernier calipers, the widths of anterior teeth were measured. The mean values were gained by doing the measurement several times. SAS6.12 software package was used for grouped t test, paired t test and REG regression. RESULTS: Statistically significant difference(P<0.01) was observed, when comparing the widths of anterior teeth with their ideal golden section(0.618 or 1.618). There were statistically significant differences in the correlation coefficient of anterior teeth(P=0.0001). CONCLUSIONS: The golden section is not found in the anterior teeth. There is some proportional relationships between the upper and lower anterior teeth.

[Clinical evaluation of fluor protector and glass-ionomer cement used as pit and fissure sealant for preventing pit and fissure caries in children].

PURPOSE: To study the clinical effect of the fluor protector and glass-ionomer cement used as pit and fissure sealant for preventing pit and fissure caries in children. METHODS: 622 health permanent teeth in 6-8 years old children were divided into three groups. Children in the experimental group A (n=207,335 teeth) underwent fluor protector every six months, experimental group B(n=205, 327 teeth) with glass-ionomer cement used as pit and fissure sealant and children in the control group(n=210, 354 teeth) underwent no treatment. The incidence of caries were compared among the three groups using SPSS10.0 software package after 3 years. RESULTS: After 3 years, the incidence of caries in A and B experimental groups were lower than in the control group, the difference was significant (P<0.01), but there were no significant difference between group A and group B (P>0.05). CONCLUSION: Fluor protector and glass-ionomer cement used as pit and fissure sealant also have good clinical effect in preventing caries.

[Clinical evaluation of two light cured pit and fissure sealants for preventing pit and fissure caries in children].

PURPOSE: To evaluate the clinical effect of two light cured pit and fissure sealants for preventing pit and fissure caries. METHODS: 416 health permanent teeth in 162 children aged 6 to 8 years old children were divided into two groups, enameloplasty sealant technique (EST) and cup-shaped brush sealant technique (CST) were used in two groups, respeitively. 90 and 112 contralateral healthy teeth were used as self controls respectively. The rate of the sealant reservation and the incidence of caries were compared in the two groups, and the clinical effect of the two groups was compared using SPSS10.0 software package for Chi-square test. RESULTS: After 3 years, the rate of the sealant reservation and the incidence of caries of EST were 85.4% and 0.9% respectively, the rate of the sealant reservation and the incidence of caries of CST were 63.8% and 6.7% respectively. Statistical analysis revealed a significant difference (P<0.05) between the two groups. CONCLUSION: EST and CST both have good clinical effects, While EST is superior to CST in preventing pit and fissure caries.

[A three-year clinical evaluation of ceramic optimized polymer posterior inlays].

PURPOSE: To evaluate the clinical results of ceramic optimized polymer (Targis) posterior inlays. METHODS: 345 type I cavities were divided into two groups: 170 in Targis inlays group (42 premolars and 128 molars) and 175 in control group (composite resin inlays, 45 premolars and 130 molars). The clinical effects of Targis inlays and composite resin inlays in posterior teeth were compared in marginal discoloration, marginal adaption, secondary caries, anatomic shape integrity, fractures of tooth and color match 3 years later. The data were analyzed using Chi-square test. RESULTS: 3 years after treatment, 166 Targis inlays were followed up, 2 had marginal discoloration, 158 had good marginal adaption, 162 had anatomic shape integrity, 1 had secondary caries, 161 had color match, and 5 had fractures of teeth. 169 composite resin inlays were followed up, 27 had marginal discoloration, 134 had good marginal adaption, 150 had anatomic shape integrity, 20 had secondary caries, 164 had color match,5 had fractures of teeth. The marginal discoloration, marginal adaption, secondary caries, anatomic shape integrity of Targis inlays were better than that of composite resin inlays (P < 0.05), but fractures of teeth was not different between inlays in the two group (P > 0.05). CONCLUSION: Targis posterior inlays is a good newly developed prosthesis.

[The effect of teeth brushing education on oral health of school children].

PURPOSE: To investigate the state of oral health of children after education of teeth-brushing. METHODS: The experimental group included 145 pupils in school after education of teeth-brushing for two years. The control group included the 147 pupils in school without education of teeth-brushing. The prevalence rate of dental caries, caries average, the gingival index and the oral hygiene index-simplified were examined and statistically analyzed by chi(2) test and student's t test. RESULTS: The indexes of the prevalence rate of dental caries, caries average, GI, OHI-s in the experimental group after teeth brushing education were better than in the control group (P<0.01). CONCLUSION: The education of teeth-brushing improves the oral health of children significantly.

[Observation of the change of blood pressure in 835 patients during extraction of teeth].

PURPOSE: To observe the change of blood pressure during extraction of teeth. METHODS: Patients without hypertension who needed extraction were randomly selected, tooth extraction was carried out. The blood pressure and the patient's response to operation were observed and recorded during the procedure. RESULTS: The blood pressure in 825 patients increased significantly. The average increased blood pressure was 45/26 mmHg, 3 patients had arrhythmia, 5 patients had adverse reaction. CONCLUSION: The patient's blood pressure must be fewer than 140/90 mmHg, before a difficult extraction of teeth.